| Relationships |
|---|
| The mRNA, DCAR_017082, is a part of gene, DCAR_017082. |
| The genetic_marker, MK007720 TP136691, is located in gene, DCAR_017082. |
| The genetic_marker, MK003684 K1128, is located in gene, DCAR_017082. |
| The genetic_marker, MK009606 5_11329307, is located in gene, DCAR_017082. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_017082 | DCAR_017082.mRNA | mRNA | DCARv2_Chr5:11325347..11331183 |
TTTACAATTTTCATTTTTCAGTTGCAAACCAAATGGGAGGGAGTAAGAAA
CACTGGAAAATTCTATCCATGAGGTTTCAGCAATAGATATCACTGTGCCC
ATGTCCATGTCTTCTTCTTCTCCCCCACTTTGGTTATTTTCATGCCCTTC
ATTTTCTTCCACTTGTAATCTCTCATCTCTCTGCATTCTTTATCATAATC
CTCAGACCATTTCCTTAAGAAGTTTTAACATTTCAAGGTTTAGACTTTTT
TGTTCCCATCAAACACTTGAATTGGATACCCAAAATGTTAAGAAAAAGAG
AAAACCCAGACCCAGTTTCTTGGAACAAATTGAAGATAAATGGTCTGTGA
AAACCCCTTGTTTGACCCAAAAATTCCCATGGGAAGAAGATGGTCATAAG
AAAGGAAGTACTCAACAATTGCCTGAATTTGCTGCAACTGAGTCAAGAAT
TGATGATAACTCGGTGGTGAGTGAGTCAGTCAGTGTTGGATTTGTGAAAA
ATAGAGTGAAGTTAGCTCAGTGGAATCAAAGAAGCAAAACCCACAAAACC
CATATGGATTTTGAAGCTGAAGAAAGTAGTGGTGTTAGAGGCAAAAGGGA
TGGTGAATTTGCTGGGACTGAGTCAAGAGTTGATGATAACTCGGTGGTGA
GTGATTCAAGTAGTGTTAGTTATGTGAATAGACAGGCAAGGTTATCTCCT
TGGGATCAAAGAATCAAACCCAAGAAGGCCCAAATTGATTTAAATGGTAC
AAGTGTAGAAAGTAGTAGTGTTAGAGGCCAAACGATTGATGAATTTGCTG
GGATTAAGTCGAGAATCGATAATAATATGTTGGTGAGTGACTCAGTGGAT
GTGGGTAATCAGGTAAAGTTTGCTCCTTGGGATCAAAGAAGCAATTCCAA
CGAGGGCCAAATCGATTATGAAGCTGGAAATCTAGAAACTAGTACTGTTA
GGGAAAAGACGGTTGACGAGTTTCCACAGGGTTATCTTCTACAATCGAAG
AATTTAGGTATTGTTCTCGAGAAAGATGATACCGTTGAAGAAACTGATAA
TGTGGATGATGCAATTTCAAGTGAGTTACTATTGGAGAAAAATGACAGGA
AATTTGATAAGCTTGATGAAAAGTTATCTTACGAAGGGATATTATCTGGT
ATTATCCAGCAAGAAGTTGGAAGGGCTGGGGATTCTATAGATGGATGTGA
TATAATGGGGCCATTGGATGATAAATTAGGAGAAGAAGATAAACTGAAGA
AGATGATGCCTTGGGAGCCTATGGAATCTACGAAGGAGGAGTCTATGAAG
AGCAATACAATGTTGGCTGAAAAACTAATTCCGGAACCTGAACTGAAGAG
GCTAAGAAATGTGTCATTAAGGATGGTAGAGAGGATCAAAGTTGGAGCAG
CTGGCATCACGCAGGCCCTAGTTGACTCTATACATGAGAAGTGGAAAGTT
GACGAGGTTGTTAAATTGAAATTTGAAGGACCTACTGCTGTAAACATGAA
GAGGAGCCATGATATCTTGGAGGTTGGTTTTATAATGCTCCTGAACTTGA
CACGCCTAAATTTTATGGCCACCCATTATTAAATTTCAGCAACATGTTGT
TGATATATATATCACTTGTATTGGTAATTTTATGAATGAACTAAATTGCA
CTATCTGATGATTCTGCTATATGATTAGTATGTTGTGTGATCAGTAAAAT
GTAGCTAGTAGGCAAGAGTTTTTAACAATGTCTTTTCCAGTGTTATGATT
ACACTTGTTTGTGATTTCAAAGATATCTCACTTTAAGAGAGTTTTTAAAT
TCTAAACTTGCTGGCTTTGAGAATGTTCAATATTTTAACATGTTGGTTTC
TTAATCTCAGCGAAGAACTGGAGGTTTGGTGATATGGAGGTCAGGTAGTT
CAATAGTTTTGTTTAGAGGAATGGCCTACAAGCTTCAGTGTGTCAAATTA
TTCACCAATAATCAGCCTAATGTTAAAGTATCACAGCCTCCAAAGTATTT
CGAAGATGATTCTCCAAATGTTACTCCCAGCAGCTCAGGTAGTTCTTCAG
AGGCATCAACACCCAATTCAGCAAGTTACACAAAGGGTCTTTCTGAAGGT
GGTAACATGGATCTAAGTGAGTTGAACTTTCTGTTAGACGAGATTGGTCC
AAGGTATGTAGATTGGGCAGGCCGTGATCCGCTGCCTGTTGATGCTGACT
TACTTCCAAATGTAGTTTCTGGTTATAAGCGTCCATTCAGACTTCTTCCA
TACGGGATACGACATTCTTTGAAAGACAGGGAGATGACTTATATCCGCAG
GGCTGCCAGGAATATGCCTCCACATTTTGCCCTGGGTATGTGGAAAATGA
ATGTTAATTATTCACACCTAAAAATATGTAATCACCTCTGTTGTCATTCT
TACATTCTATAGGACGAAATAGAGAGCTGCAAGGCCTGGCTAAGGCTATG
GTGAAGCTGTGGGAAAAAAGCGCCATTGCTAAGATAGCTATCAAGCGCGG
TGTGCATAATACTCGCAATGAAAGGATGGCTGAAGAACTCAAGGTAAACA
TACCATTTTGTTGTGCATAACTGCATATGCCTGTGGTTCTTTGTTCTGTT
TATCATGCATGCACATCTGGTGTTTGCTTCAGAGTTGTGAATGTTTTGTA
TCATTCTAGATTTAGTTCCTAATTTCTATGGTTAGAAACACTTTATTCAC
AAGCTATATTGCAACATACAAAACTGTGTCAAAGATAAGGTGGACTGCTA
TTATATATTATGGTATACTTCAGAAACATTGACTTTTGTAACTTATCAAT
TTGGTAAAGATCTATTTGGAAAGATATTGTTCTATGCCTCAATGAAACTA
CTAATTATGTATATGTATATGTTTTACACCGTCTATTAATAAGTGAAACG
CTGCTTTAGTGCATCAGAAATATCAAATAAAAAGTTTTATATCTAAACAA
CAATAATTTAAATAAGTATATTAAAAAGATGATAATGTAATCAAATAAAA
AGACATTATATTTCGAAAAAATAATATATTTAAACGAATTTTCTATGGTG
TGCCCAAGGGCACACAATAGTCACCAACTCTCATGAAAATTCTAACTTTT
GATTGGTGGATAGATAATAAATGTAAATGGCCCCCCTGCATTCACATCAA
TTCCACCAATCTAAACAAGCCATTTTCATGAAATTTGGTGCTTATTGTGT
GCCCTTGGGCACACATTAGAAAGACCGATATTTAAAATATTTATTTAAAC
CCGTGCTATGTGATTCTCGGACTCTCTAGAAAACCCATCCGTACCCGTGT
CTAGAGGACACAACACTAACACCGGGATCCGGATCTGGACGTCATCCAAA
AATCAGCCTCTCCATCTTAGACAACCTATCTTTAATTTTAGTGACCTATC
TCTAAGAGGAAGATAGGAATAAGAACATATATGAAGAAACATAGGTGAAA
TGGATGTAAAAGGAGAAAAGAAGCAACTCTTGCAATTGTTTGTTATGCAG
TATAATAGAATGATAGTTATTTATTTATAAGAATAATTTAAAATAATAAT
GCAGTGTCCAAATCCAAGTGTCGTATCCTTATAATCCCCAAGTCTTTAAT
TTTGTAGTTCAAAAGATCTGACAACCTGATATGTGTTTGTTTCGGATCCT
TCTAAAAGAGTCTGAGCATCATAGTCCGTGCTTGGCACGGGTCATAAGCT
AATGTTCAAGTACAACAAAAATACTTGCTTTCTTATAAATATAAACCCGT
GTTACATACATTTAGGATCTTGATGCTTGCATCCGCAATACTGAGATGTG
ATCCAGATTACCCTAATGTTTTAATATATTGTATCCGGTTTTACATTCTT
TCAGCTCATACAAATTTATATCATTTGTGCAGATATTAACTGGGGGAACA
CTGATATCTAGAAACAAGGACTATATTGTCTTTTACAGGGGTAATGACTT
CTTACCCCCTGATGTAACACAGACGCTGGTAAAAGCGCAAAACCTAGCTG
CTATCAACCAGGAAGAGGAAGATCGGGCCCGTCAAAAAGCGTTTGATCTT
ATGGAATCAAATACCAAAGCTTCTAAGGAACACCCGCTGGTTGCAGGAAC
GCTTGCCGAGACCATGGCAGCAACCTCACGCTGGGGAAACCAGCCAACCG
CTGAAGAAAAAGAGAAGATGATGAAAGACTTAGCTTTGGCTAGACATGCT
TCTCTAGTTAGGTTTCTTGAGAAGAAGCTTTCCCTTGTAAGTATCAAATA
CACTAGTACGAGTGTTCCTTTCGTTACCTGTAAATTACATCGAAACATCA
CTCCATATATGACGTTTAATGTGCTATAATAATTCAGGCAAACAGGAAAG
TGAAAAGGGCTGAGAAGGCTTTACGAAAGGTGCTGGATTATCTGGAACCA
GCTTCACTCCCTGATGATCTGGAAACTTTAAGTGACGAGGAAAGATTTCT
ATTTCGTAAGATTGGCCTGAGTATGAAGCCTTTTTTGCATTTAGGTAATC
ACTCCCACTTTCGTAGTATCCTTCTGATATATAATAAGAGCTGAGATTAA
TATTATATGGTTTTTTTAGGGTTAAGAGGAATTTTTGACGGCACCATTGA
AAATATGCACTTGCACTGGAAGTATCGCGAGCTGGTAAAGATAATAGTTG
ATGGTAAAAGATTTGCTCAAGTCAAGCATATTGCTATTGCTCTCGAGGCA
GAAAGTGGTGGAGTATTAGTATCTGTGGACAGAACTACTAAAGGCTATGC
AATTATAGTTTATCGTGGAAAAAATTATAGGCGACCCAGTGCCGTGAGAC
CCAAGAATCTTCTTACCAGAAGGCAGGCTTTAGCTCGATCAATTGAACTT
CAGAGACGTGAGGTAATAGAAATCTGAATCCTATGTCAAAGAAGAGGTTC
TTCCTCTCAAAATATAAAAATTTTGCAATCGGACTGAATATTAAGAGGGA
ACATTTTTACCTGCATTCTTTATATCTCAATTAATTATGTTCTGGGGTGT
TGGTCTATTTCTGATCTATGTCCAAACATTTTCAGGCACTAAAGCATCAT
ATTACAGAGTTACAAGCAAAGATTAAGAAACTCAAGCTTGAGCTGGTTTG
TTTTCTCCTCACAATTAACATGCTAAGACAGACAAATATAAAGAAGCTCT
TAACTTATTATAACATACTTTTTATGGACTTCTGTTATAAAATTTGCAGG
AGGATATGACAGTCGTAAACAGGATTAGTGAGGATACCTTTTACTCAAGG
GTAGATAATGCTTCTGACAGTGATGAAGATGATGACGACAGCATGCAGGA
GGTAAATATAACTGATATTTTGCTTGCTTAAACATATTTTTCCTCAATGA
AAGAACATTATGTTGTGTCTGGTTAGGGTGATTGGAATGAGTGAAAATGA
AAGGAAATAATTGAGGGTAGAAGGGAGGACTTGAAAAAAATGAGTGGATG
CAAATTTTGTGTGATAAGACTTGGGTAGAAAATAGGTATGATAAGGAATG
GAGCTTTGCTCCATAAAGTGGAAGGCTTGAGCTCTCGTTAAGGTTGATAG
GAATGGAATGGTGGAGGGAATGAATTTATTAAACATTTAACTAAATTATA
GTTCAAATTCATTCATTGCCTCCATTTTCATTCTACCCCAACCAAACACA
ACATTAGTTTCTGTTTTAATCCTTAAAATATCAAAGAATGTGGACTCTAT
AATTGGACTCTCAAGCTCTAATTTTTCCTGCAGTAGCTAAGCATCATTCT
AGAGCAGTGATGATCAGTTCTGCTGATTTTTAGACTATTTAGCCCAAAAA
GGTCTGCTAGATCAACCTTGATCGACATGATATTGGTTTTGTTTCTATGG
TGCAGGACGACGATGAAGAAGCGTACCTTGAAGTGTATGACAGCGGAGAT
GAGGATGGTAAATCTGAAATGGCATTTGGACATTGATGAGAGCAAGAAAA
AGATGCATTCTCCTAGATATTCATGATGTACTAGAATCCTTGTGCCCATA
TTATGGTTAGTCATCTGAATATATTTACCAGGCCTCGGCATGCATATTGT
GATAATTCCTTTCTTATGTTAGTGTACGGCCAAATTTAGATCTACCTGAA
ATAATGTTAGTCTCGAGCTTGTAAAGAAAAATAGCAGCAGTATATCTTCT
TTGATTTGAGTTGCGTGTATATTGTGTAATTTACTTATTTGTTCTTTTTG
TGGCAATGCATCA