| Relationships |
|---|
| The mRNA, DCAR_022010, is a part of gene, DCAR_022010. |
| The genetic_marker, MK003296 K0740, is located in gene, DCAR_022010. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_022010 | DCAR_022010.mRNA | mRNA | DCARv2_Chr6:17595214..17602198 |
ATGGAGATCGCACCGGCGGTGATATCATCCCACGGCAGCGTTGATAGCGG
CGATATCATCGACGAATCTCTTAAGCCATCTGATTCAAACGCCTCTTCAT
CATCACAAAATAATATTAACAACGAGAATATGAATAACAATAATACAATT
AGTAATAATATGATGAATAGATTTGATGATGATGATGAAGAAGGAGATGT
GTGTCGGATCTGCCGGAATCCAGGTGACGCCGATAATCCTCTACGGTATC
CGTGTGCGTGTAGTGGAAGTATTAAATTTGTTCATCAGGATTGTCTTCTT
CAGTGGCTTAATCATAGCAACGCTCGCCAGTGCGAGGTATATAATCTCTC
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTATCTCTCTCTCTCACAC
ACACACATATGTATGAATTTGTGTGCGGGTACATGGACTTCTTTGGTTAC
TGTTTGTATGATTGTATTGTTATAATCAGATTAATTAAGTTAGGCTTTTA
TCAGTGATGTTAATTATACCTATGATGAATTATTTGATGGAGTCATGGAG
TGAAAGAGGAAAGTTAGTGGCAGACTGAAGATTGCAAGAGTATATTAGGG
AATGCAATTTTAATATTTTATGGATATCGAAAACATAAATCAGTTTATTC
TAACTGTTATTATAGAATTTGTTACAACACAGCTTTTTCTGAGGTGCTGT
TTGTTCAATTGGTATTCATTGGTCAAGTCACATCCTTAAGTATAATATGG
CACTTGTTTTGGTCCATTGTATTTGCAACGCACCATGTAAGAGGTGGCTT
TTAGGGTGGCAGTTTTTGAGCTTGTGCTAATGTTAGAGTAAACGGGTAAC
GTGTTTATTAAGTTAGAGCCTCCTGGATGTGCATGTCATTAACTTGTATC
TGTACCTTCTTTTTGTCGTGTTATGGAGTTCATTTACTTGGTCTGGAGTC
AATTCATGAAGTTCGAGTGTATAGAGTTAAACATCATAAGATCCTATAAC
TAGAAAGATGTCAAAGTTTAAAGGTTTTGAAAATTAGCTCAGTAAATGTC
TAAATGATATTGAACATCTACAGTCCTTTTATGGAATATCGAAGGATATA
TGTAATGTGTATATGACAATATAAATGAGATCCTTGTTAAGGCTTTAGGG
CCTATATCCTTCTGATGCTGTGTCTGGGCTGTTCCCTCTAGATTTCAGTG
ATTTTCGCTTCATCTGTCTCTAATCATACATATTATGTTGTATTCAGTGT
GTGTTGTTTATTGGTTGTCTACGTTTGTGCTGCAGGTGTGCAAGCATCCT
TTCTCATTTTCCCCTGTTTATGCTGAGAATGCTCCAGCAAGGCTTCCGTT
TCAGGAATTTATAATTGGGATAGGAGTGAAAGCATTCCATGTTCTCCAAT
TTTTTTTGCGCCTCAGTTTTGTTCTCTCTGTCTGGCTTCTAATCATACCA
TTTATTACATTCTGGATATGGAGGTTGGCATTTGTTAGGAGCTTTGGCGA
AGCCCAAAGATTGTTTTTCAGTCATATAACTACGACAGCTGTTCTTACAG
ACTGTCTACATGGCTTCTTACTCTCAGCTAGCATTGTCTTCATATTTCTT
GGAGCCACTTCTCTTAGGGATTACTTTAGGCATTTACGAGAAATTGGAGG
ACATGATGGTGAGAGAGAAGATGAAGGAGAAAGAAATGGTGCTCGTGCTG
CTAGAAGGCCGCCTCATGTACCAGCAAACCGGAATCTTATTGGTGATATG
AATGCCGAAGATGGGGGAGCACAAGGAGTTGCTGGGGCAGGTCAGTTAAT
CAGAAGAAATGCAGAAAATGTTGCTGCTCGATGGGAAATGCAGGCAGCTC
GTCTAGAGGCTCATATGGAACAGATCTTTGATGGGATGGATGATGCTGAT
GGTGCAGAGGATGTACCTTTTGACGAGTTAGTCGGCATGCAGGGGCCTGT
TTTTCATTTGGTTGAGAATGCTTTCACTGTGAGTATATATCTTATTGAAA
TCAAGTTCTGATTAGCTATTCATCGTTTACATATCATGTAGATACTTCTT
TGGCTGGTTCTTTGTTCTGGTTTTAAAACTATAGTTGATGTAGTATGAGA
AACGGGTAGTTCATGCAAAATTAAGTTAGTATAGGCATGTAGCACAAGTG
TTGGAAAATTTATCTTCATTTGAGAGCATAAGTTTTTGAAATATTGTCAC
TGCGGTGGCAATCAACCTTACTCTAACCGGCTTATGAGACTTGTTAAGTT
TCGTCCATCTGATTAGCAAAAATAATGTTCTCTTTGAACTGGTTTCTTAT
GTGGTTTTTATCCTCTTTTGCAGGTTCTTGCGAGCAATATGATATTCCTT
GGTGTTGTTATATTCGTGCCTTTTTCCCTAGGACGGATTATACTCTATTA
CCTATCGTGGCTATTGTCCACGGCAACGACTCCAGTATTGTCAACAGTTG
TACCTCTTACAGAATCGGCTCTTTCTTTGGCCAATGCTACTCTTAAGAAT
GCTTTGAATGGAGTTGTGAATATGACATCTGGTAGCCATGAAAATTATAT
CAACCAGTTTGTAGAAAATGTGAGTCTAAATGTTACAGGGCTGACCAACC
TATCAAATAACGGAACCACTACAGTTTCAGCAGACATTTTAAAAGGTGCA
GGTATGGGGGCCTCACGGCTCTCTGATGTCACAACCCTTGCTGTTGGCTA
TATGTTTATATTCTCGTTGGTCTTTATGTACCTCGGGACTGTTGCTTTAA
TTCGGTACACTAAAGGTGAACCTTTGACTATGGGGAGGTTCTACGGCATT
GCTTCGGTTGCGGAGACCATTCCATCCCTGTTCAGACAGTTCTTGGCTGC
TATGAGACATTTACTTACCATGATTAAAGTTGCTTTCCTTTTGGTCATCG
AACTTGGTGTTTTTCCTTTGATGTGTGGGTGGTGGCTTGATGTTTGTACC
ATAAGAATGTTTGGGAAGTCTATAGCCGAGAGAGTGGATTTTTTTTCGGT
TTCCCCATTAGCGAGCTCGTTGATTCATTGGGTTGTAGGGATTATATACA
TGCTACAAATAAGTATCTTTGTCAGCCTTCTTCGAGGGGTATACAATTTT
TCTTTAGTGATCATTACTGTGTTTGTTTTAGCTTGTCATAGGCTATCTAA
TACTCCGTTCCATGTATAGGTTTTGCGCAATGGGGTTCTTTACTTCCTCC
GTGATCCTGCTGATCCTAATTACAATCCTTTCCGTGATCTGATTGATGAT
CCTGTTCACAAGCATGCTCGCAGGGTTCTGTTGTCTGTTGCTGTTTATGG
AAGTCTTATTGTGATGCTTGTGTTTCTACCGGTCAAACTTGCCATGCGAG
TAGCTCCTTCAATCTTCCCGCTCGACATATCGTAAGATATTCTTTCTCTT
GGTATATAATTTTTTGACTTTTCTCCTTATGTTTGGCTTGATGAACCTTG
TTGAATTTAACTGTTGATCCTTGCAGCGTATCAGACCCATTTACTGAAAT
TCCTGCCGACATGCTTCTCTTTCAAATATGCATTCCGTTCGCACTTGAGC
ATTTTAAGTTACGTAAAAAGATCAAATCCCTTCTTCGCTATTGGTTCACG
GCCGTTGGCTGGGCACTTAATTTGACAGAGTTTTTACTCCCACCTCCTGA
GGATAATAGTGGCCAGGAACATGGAAATGGGCAGCCACTAAGGCAGGATA
GACAGCAAGCACAAATAGGCGGTCAAGATCGAGCTTTTGGAGGGATTGTT
GCTCATGATGATTTGAACAGCAACAGGCATTTACCTGCTAATGCTACTTC
TGCAGAAGAGTTTGATGGTGATGAGCAATCTGACTCGGAGTAAGTTTTAT
TCAAGTTTGCAAATCATTTTATATAGAGAATTTAGTGTAATGTATTTTAC
ACTCAGTTTTTGTTTTCATAATGTATAAAGTAGCCAAAGCTGTGGTATAT
ATATTCTTATTTCTTTTTAAGATTAATTGTATGAATCAATAAATTTAAAG
TCGCTTTTGTTTCCTTGTTTGTCAAGCATAATGTCTATGTTGAGTCATTT
AATTGCAAAACTTGCAAAATACCTTTGTGGTCTATGGGAACCTGGATTTT
ATTTGAAATTCTGAATTTGAACAAATAACCTGTTTGGGAATATGGATTTG
GATTTCATTTGAAATCCAGGACGTTCAAATATTAGTATAAACCTGAGAAT
TTGAAATGACAACCAAAATCCTGTCATTTGAAATGAAATGCATGTTTCCA
AACGCCCTCTTGTATGATTTGATTAAAAATGCCATGGTACAAGATCCCTT
TGCCTTATGTGGAGCATTGTTTTTGGACCCTCACTCATTTTTGTTTTGGA
AAATTCCCTATCCTAAGCTATTATGTTGAGTGAAAGAACAGTGGAAATAT
AACTATTCAAGGAAATTAACATGTTAACATACTAATAGAAGTAACCAGTA
ACATTATCTGAAGGAATTTAATGGGCATACATGTTTTCAACTAGTTGCTC
CATAATAGCTTATTTCCTCTCATGGCAAAAAAAGAAATGTTCTTAACTTC
TTATAATGTCATCTCCTGGAAATTTTCCGGGCAACTTCTCGTGTCCTTTG
TTTCTCACTCTTTGATATTTTGTCTTGGGCATCTTTCAGTTTGCTGTCTG
ACATTTGCTATAGATAAATACATGTTTATTAATGTAAAAATCTTTTTATT
GAGGCACTATGTTTTTACTCTGATCAGTAATCAATCATATGTAATCTTAT
GTTTTCCTCTCCTCTCTTATTTTATTTATAAAAAAAAATTAGAAACATTT
TTTTATATGATCTTACTTCAATTCATATATGGGGTATATCTTAGTTTGGA
CTAATATATTGTAACTCAATTCTTTTAGTAGGTACAGCTTTGTGTTCCGC
ATTGTGCTCTTGTTGGTGGTTGCCTGGATGACTCTTCTGGTTTTCAACTC
TGCTTTAGTAGTCATACCTATTTCACTGGGACGATTGCTTTTCAATGCTG
TGCCTTTTCTCCCGATAACACACGGCATCAAGTGCAACGGTAGGATAACT
GATACAACCCATGTAGGCTTTAATTGGATTTTTTTGTAGACAAAAATCTT
TAACTTTTAATTTTTATATTAGATCTCTATGCTTTCGTCATTGGGAGCTA
TGTCATTTGGACTGTTGTGGCTGGGGCAAGGTATTCCATTGAGTACATAA
AGACTAGGAGGGCTGCAGTTTTGTTGAAGCATATATGGAAGTGGTGTGGC
ATTGTTTTGAAGAGCTCTGCATTATTGTCAATATGGGTAAGGATCTGTTT
TTTGAAAAATAACTACTTTTTTACTTAATCATTCTAACTCTAAAGTCGAT
TTTTCTTCCAGATTTTTGTCATCCCAGTTCTGATTGGACTGCTCTTTGAA
CTTTTGGTAATTGTGCCAATGCGGGTACCCATTGATGAAAGTCCAGTATT
CCTTTTGTATCAGGATTGGGCTCTAGGACTAATTTTTCTGAAGATTTGGA
CCAGACTGGTGAGTTCTTTGTTGCTTCTTATGTCTTTCTTAGAGGGTATG
TAGATCTTAAATGTACAAGAGCAGGAATGCTGGGTGCACTGTTTTATGTG
GCATTTCTGCATCATTAATAAAATACATGGTCCAAGACCTTACAAAAAAT
AAATAAAAATTACATGGTTCAAGATAAAAGTTGGCCACATTCGAATAATT
TTGTTAAGAGCCAAAACTTATTGCCTGACCTAATTCTGTGTTGAATTGAC
ATGCAATGGCATGTTAGATAACATTTTTTTTTCTCAGATGGACCACAAGT
TACAGGTAGCTTCTGCTGTTAATATTTAAAAAGAATAACTAGATGACTAG
CAGCAGTATTCTTTTGTCATATCTGTATGTTCCGTCTTACTTTGAATGCT
TAAACGACTACTTCGTTTTCTTATGCTGTTTTATAATATTCAGATAATAG
ATATAATAATCTCATTTTCTTTATTGGTTGCTGATGATATATTCTGTTAA
TAGCCATAATAATGTACAAATAATTAAACCTGATTTCTGTATGTTGTTCA
GCATTCAGTTGTGTTATGTTCTTATATTGTGTCTTTTGCTATATAAGAAA
GGTCAAAGTTAATTGAATACTTAACCTCTGAAGGAGGTTTGAGCAAGAAG
GGAGTATTTTGGACACTTGACTTTATCGCTAGAGTTGTATGTTTAATAGA
AGAGAAAAGGGTTGCTCAAGAATAGTTGTATAGTATATTTGTCAGTAGTG
ATATAGGAGGCCCAAAAGGAATGTTTATTATTGTGCTTATTCTCTGGATT
TACCACTAGGTAAGCCATATGGTCTTTTCAATTAGAATCAACCATGCAAT
ATGGTAATATTTATTCTGGAGTTCTTACTTTCATGAGATCTATCCTCTAA
AAGTTCAATCAAAGTAGTTGGTTACCACTGGAATTTTTAATTGGTCTCAT
AAGCTTCATTTTATGTTCCCTTAGGTTATGTTGGATCACGTGATGCCTTT
GGTGGATGACAATTGGAGAATAAAATTTGAGAGGGTGCGAGAAAATGGTT
TTTCCAGGCTGCAGGGATTTTGGGTGCTGCGGGAGATTGTATACCCAATA
ATAATGAAGCTGCTAACTGCTCTCTGTGTTCCTTATGTATTGGCTAGAGG
AGTCTTCCCGGTTTTTGGTTATCCATTGGTTGTGAATTCAGCTGTATACA
GATTTGCATGGTTGGGATGTCTAGGTTTTAGTTTGGTGTGCTTTTGTACC
AAGAGATTCCATGTGTGGTTCACCAATCTCCACAATTCGATACGTGATGA
TCGCTATTTGATTGGTCGTAGACTTCACAACTTTGGAGAAGATAAAGAGG
TGAGACGTAATGACCCGGATGTATCTTCAGAAATTCAAGATGCTAACATA
CAGGACCCTACAATGGTCCTACATGAAGGGGATGAAGCTGATGTAGGGAT
GAGGCAGAGACGTGCTATAAGACAAGATACCTAA