DCAR_023329 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_023329

Identifier:

DCAR_023329.gene

Locus Tag:

DCAR_023329

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 4 relationships.
Relationships
The mRNA, DCAR_023329, is a part of gene, DCAR_023329.
The genetic_marker, MK008856 85036.37770, is located in gene, DCAR_023329.
The genetic_marker, MK011324 7_527151, is located in gene, DCAR_023329.
The genetic_marker, MK008857 85036.16321, is located in gene, DCAR_023329.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_023329	DCAR_023329.mRNA	mRNA	DCARv2_Chr7:526440..533896

Sequence:

ATGTCGTTGAGACCCAACGCGAGGACCGAGGTTCGTCGGAACCGCTACAA
AGTCGCCGTCGATGCCGATGAAGGTCGGCGCCGGCGAGAGGACAATATGA
TCGAGATCCGGAAGAATAAGCGGGAAGAGAGCTTGCTTAAGAAGCGTCGA
GAAGGCCTTCAGGCAAATCAGTTCCCTCAAGCTCCTGTCGTTGAGAAGAA
GGTACTTCTCAAGCTTCGCTTCGATGTTTTTGTTGGATTATATTGATTTA
TTTGAATTTGTGGTTGATTAAAAAACGTGATGATACTAGTTTTGGTGTAA
TTGTGTTGTCTAGTGCTTTTGAGTTTAGTTTCGAAGATTTTTCTGGTTAC
ATGTGGAATTTGTGGTTGATTTTTAATATGAGATTAAGATTTAGGTTTTA
ATTGTGTGGATGTATGCATAGGAGTGTGTGAAATTTGTGTATGACTGGTT
TGGAAGTTTGTATCAACATTTCTGGTTGATTTAGGATTGATATGTATCGA
TATTTCTGGTTGATATAGGATTGATAAGCTAAAGATTTTGGCAAAGATTG
TTTGTATATGTAGGATTATGTGGAATTTATGTTTAATCGAGTTTCAAAGT
TTATATTGATTATGTGGTGTTTGTGGTTGATTTAGACTGACAAGTTTAAG
ACTTAGGTTAAAATCAGGCTTACATAGTGCTTTGGGTTCAGTGTTGAAGT
TTTTATTGGTTATATGCAGTTTGTGGATGATTTAACACTTAAGATGTAGG
TTCAAATGTGTGTTGCATATAGGATTTTGTTTTTATCTGGTTTATTTTAA
TGAACAGATTCGACCAGCACTGAGTTTTCTATATGTTCGTAGTCAAAATA
CTGTTTTTAGTGTTAGAGAATGTTATGTATGTGAACTACATACAAACTGT
GTTTTTGTATTCAGATTATGTGGTGGTTTAATATCTATAAATAGTATTCA
GTTTAGTGAAATTTGGAGCCCTCTCTCTGTTCTCTTTTATATATTCATGT
GTGTGTACACCTGGATGTAAGTATCTAATCTATTGCTAAGATGGTTTCTA
GTCGAATATGACTGTAAATGTATATTCAACGAATAAAGGAAAATTTTAAT
ATACATCATTCTGACTGTGCTGATAAATTGATAATACTTGACAAATATAT
TAAATTTGTCTGATGGTCAATTGTTTATGCAGTTGCAATTGGAGAGTATT
CCTGGTATGGTTGCCGGGGTGTGGTCCGATGATAGTAATATGCAGCTGGA
GGCAACCACTCACTTCCGAAAATTACTTTCAATAGGTATTAAAAGAAGCA
TGTAACTTTGTCGATTATTTCTGGAATTATGATCGATGCAATTATGTAAG
ACAACCTTGAGTATTTAACAAGTATTAATTGACAGAGCGGAGCCCTCCAA
TCGAGGAAGTCATTCAATCTGGAGTTGTTCCACGTTTTGTGGAGTTTCTC
ATGAGGGAAGACTACCCACAACTACAGGTTTGTGATATCTTGTTTTCGTG
CTATATACTAGATGTATTTTAGTTCTTTCTACTTCATACCAATTTAAATG
AGCTTTTTGCAAAAAAATTTATATTCCAAATTAGCTGATCCTTTTCTTCT
TTCAATGTATAATTACCAACAATCTTCCAAAGTTAGCCCTTGTTATATAT
CATTTCCATTGTAGCCTCTTTGAAGTTAATATAATAAGTATGGGTAGATG
TAGAAAAATTTACAAGTATTTAAAAGTGGTGATTGACCACGGGTCATTCC
TATCTTTCTTATAGTTTGAGGCTGCATGGGCTCTCACAAATATTGCTTCT
GGAACCTCTGAAAATACCAAAGTGGTGATTGACCACGGTGCTATTCCTAT
CTTTGTGAAGCTTCTTGGTTCTCCAAGTGATGATGTTCGGGAGCAGGTGC
CATTTAAGAATTAGTGATCCATATATGTATATATATTAATGAATATTATA
TCCTTGTGAATGTCTTGGATAATCCTTTTTTAACTTGTAGGCTGTTTGGG
CATTGGGAAATGTGGCTGGTGACTCTCCTCGATGTCGTGATCTTGTCCTT
GCTAGTGGTGCTTTGCCGCCTTTGCTGGCTCAGTTGAATGAGCACGCAAA
ACTTTCTATGCTAAGAAATGCTACCTGGACGTTATCGAATTTTTGCCGTG
GCAAGCCACAACCTCCTTTTGAGCAGGTTGTTTGTGGGTCTGTCTTATAG
GTGTTTGATTACCTTTTTTTTTCCCTTTCTGATTGTGGATTATTGAATAG
TTTATCTGGGGTTATCTTGCAGACTAGGCCAGCACTTCCTGCTCTCCAGC
GCCTTGTTCATTCTAATGACGAAGAGGTGCTGACAGATGCATGCTGGGCT
CTATCATATCTCTCTGATGGTACTAATGATAAAATTCAAGCTGTCATCGA
TGCAGGTGTCTGTCAACGTCTTGTTGAGCTTCTTCTGTAAGTGTTATATA
ATAAAAAATCCTGATTGAACACTTCTGTTTGTGTGCATGTTTGAGTTTGA
TACTTTGATCTCATTGTGTTATATGGTGTTTCAGTCATCCTTCACCATCG
GTTCTTATTCCTGCACTTCGAACTGTGGGGAACATTGTGACAGGGGATGA
TGTCCAGACTCAGGTATGGTCTGAACTAAGATACACCAGTTAGAACGAAC
ATGAAAACACCAACTAGCTTATAAAGTTGATATGAAACTGGCTCTTTAAT
GTGTTTTTCTGGAATTCAGATGCTTAAGTGATTTATGTGGTTTTGCTCAA
CATTTTGTCCGTCTTACTTTACAACAAGACTTTGATGCTTAATAATAGCC
CCTTTTATAGGGGGTAGTTGCATTAGTATAACCTAATATATACCGTAAGC
TGTCATTTTATTAAATTCACAATTATGCTTGGTTTATATCTGGTTTGAAC
TATATAATTTGTGTGAACTGTTTAAGTTCATTTTTTGTTACGTCTTTGTA
ATTGCAAAAATAATATCTACAACATGAACATATAATATTTCATTTTTGTA
CTTCATTTTCTTATCTATTAACTGGTAATTAGTTAAGGTGTCTCCTGGCC
AGACAAGGTTGTTGGTGTGTTTCAGTTATTGTTTTGTGAATGTGGTTTTC
AATTTGTTACCAGTACTAGTACTGCGAAGCACACACTAAGAACTATGAAA
GGTATTAAAATATAAGAATAAGTATGTAATGAATAACTATAGTAAGTTTT
TCATTTGAAATGCCGACAACCATGCAGAAATATTCCATAAAAAATCGCTA
TAATGCTAAAATGTAGATCGCAGAGGAAAACGATATCTAAAAGAATTGTG
TGCAAGTTGTCCCAAAGATTTGCACTTCTGTTAATTATTCGGTAATCAGT
ATTGGACTTTGTGCTCGTGTACGAATAGGAATATTTGGGTGAAACCAAAG
TTTTTTCACAGGCTCTAATTCAAGAAATAGGCATGATTTCTGCATTAATT
GATTTCCTTGTGGAAGACGTTTGATCTGCAATTCTGCATGTCTGGTTATT
AGCGACTAACTTGTGTAGATCCAGCCAAGAGAATTTAGTCTTAATGGCAA
ATCGATTTAGTCTTGATGACAAATGTTAAGCCTTTGGAAGGCCACTTGTT
AGAATATTGCTAATCAGATAATAAAAATATTACAAGGGTCTTTATGGTGT
GTGCCCAAAGGCACACACTAACAACTACTTTTTACTTGGGTGGCAGATCT
TTGTTGGTGGAGATTTTTGTGCATGCAGGGATTCATTATGGGATGAGAGT
TAATAAAAATACTACACCTAATCAAAAAGGCTTTGTTAGTTGTGTCTATG
GGCACACAAAACATCGATATTTATGTATGGATGTAATTTATGATGTAAAA
TATATAACTAATTTGAATTATTTTCTGCTTCGCTTTACCTTTACTTTTAG
TTGGTGGTTGCATGCAAATAACATGGTTTAGCATATTTTTTTTGGTTGTT
TTTTAAGAAGTGAATAATGCTGTGGTGTTTTCTTGTTCCAGTGCATTATT
AACTTTGGTGCTCTGCCTTGCCTGCTGAGCTTGTTGACCCATAATCATAA
AAAAAGCATTAAGAAGGAAGCTTGCTGGACGATTTCAAACATTACTGCTG
GGAACAAAGAACAGATACAGGTTTGTGTCTATAGTGTTGCTGGTAAACAG
GAGCAGAGGCTTTACTACATATTTTTTATTTATGCAAAATAGATTGCTTT
GTTGCTTCCTCTTTTTGTTGAATTATTGTTTTAGAGTTGTTTTTTTGTTA
TATGGCCGCCAAATATATGTTAGATGTCTAGGCATAAAAAGGGGAAGAGG
GAAAGAAGAACCTGAGGTTACAGTCCTTTAAAAATATTATATTTTGTATG
TTGCGGAGAAGTTGTACTTTTTTGCATACTAGGCTAGGCACACACACCAG
GTATCGGCTGGCCCCAAACAGTTTTTCCCCGTAAAAGGACTAGTATGTAG
GCGAAGGAGCAATGATCTATAAGGGCATCTCCAATAGGCTCCTAATTCAT
TCTTTTAAAATAAATATAATATGCGACTCATAGCAAGTTAATATACTGAA
TAACGTGATAACTCCGACACTACTCTTTATACCGGCTCTCCATTTATATT
TTTATTATTATTTGAGTGAAAAGTTGGAGATAGAATTGGAGAGAAATATA
TGCAATTGGAAGGAACTAAATATTATATTTGATGATAATAAAGTTAAGAG
CGCCTAGTTGCTCTTTAAGAGAGAGGAAATAAGTTAAGAGCACCTAGTTA
CACTTTAAAAGAGAGGAGAGAGAGGAGGCTCTTAAATTTTTAAAGAGTCG
CTAGGAGCCTATTGGAGCATCAATTTTTCACATATTTGAATGTTAAGTTA
AGAGTTTGTTTGAAAAGCCTATTGGAGACTCTCTAGGGTAATTAGTCATT
TAAATCCTAGTCAATGTGACATAGGCAGTATATAATAGTTGTGAAGTTCG
ATGCACGGATCTTAAAGACCTTGGGCCTTGTAAAACTACCATGTGAGCAC
CTGATGTTTTGTTTTTAATAAATGATTTATCTCGATGCGGTATATAAGCA
GCTCTAGTTCTCCCCGTTTCATTATAAGCAGAACCTGAAATATATATAAT
TGCCAAAAATATTTGATAACTAATGTGTGGGTCGAATAATATATGTTATC
GGAAAATCTGATGTATACTTAATTTTGATAAGTGCATTCCACTTGCATAA
TGATTAGGTGCAAATTTGAGGGACACATTTATGCATGTATATTTAGAGGC
AAATATGTTGAAATTGTGTATATTCAGTACTTAAATTTGTTTTTGAAGCT
TATAGCCATTTTGTGGAATTTGTAGTTAATTTAACACTGATTAGGTGCAA
ATTTGTGGAACACATTTATACATGTATATTTAGAGGCAAATATGTTGAAA
TTGTGTATATTTAGTACTTGGAATTTTGTTTTTGAAGTCTATAATCATTT
TGTGAAATTTTGTGGTTAATTTAACACGGATAAGCTTAAGATTTAGGTTA
TAATTGTGTGTATCTATATACAGGAGTCTAATTTTATGAGAATCATTTTG
GGGGACATATTCAACACACATAAGATTTTGTATATTATGTACATTGGGAA
TACTGTTTTCTTGTTTAGTGTATATTATATATGTGAACTACGTTTTTGTA
TATGAATTATGTAGTAGTGTATTTATAATATATACAGTGAATTGTGTGAG
TAGGGGAGGTTTGTAGAATTGCCAACATCTTTCTAGCATGGTTATTACGG
CCCCAAGTTGAGTCTCGACGCCCGACCTTTAAAAAGACCAATAGACTAGG
AGACATGTTGCCTGATTAGTCCTAAATAGTGGATAAGACTTTCATAATAC
ATATAATTACTAGATTTCATATAAATATAAATTAACGCATCAATTTTTAA
AGTTTAAGCAGAAGTAATAGAATAATGAGTTCAAATTAAGCATACCTACT
ACACAATAACATCCAAATACCAAATATCAACCATGTCCAACCTAATATAA
ATTATAAAACCATATGTGATGATGAAAAACAAGCAAATATCATATCATGT
TCATTTTTATCTCCTTGTGTATTGTCATTGATTGGACCGGGTGATGGTAC
GGTACACCATAATCATGGTTATCCTGGCGCAAAGTCAAACTTTGAGGAAC
TCAGAGCCGGGAGTGCCTTTGGATCGACTAGTCGTCGACTAGTTGTTTTT
GCGATAACCATGCTCTCTAAACATATATCTTGGTAACTTATGTTTGGTCT
GATAGATATATGTAAAATCTGGGAAAAAACTGAGATGCTATCAATCATGG
TATCAACCTTGATGGCATTCCACTGGCACCATATTTAGATGCAAATATGT
TTTCACACACATTCATATATGGAATACTGTGAACATATGCTTTTTACAGC
GGTTGCTTTGTTAGCACTTTGAGATAATTTATGATGTTGCACATTACAAG
AAGTCATCCCTTGCGGAGCTAAAATGGACTTCTCTCTCTCTACACACAAT
TACATATTTTTATATGTATTTATGGTACTTGTGTGTTTGTTTTTTCTTGC
TTTTGGACATTATTGATATAACTTGACTGTAATAACAGGCTGTAATTCTG
GCGGGGTTATTTGGGCCTCTGGTTAACCTTCTTCAAAATGCTGAATTTGA
CATTAAGAAAGAGGCAGCATGGGCAATTTCAAATGCCACTTCCGGTGGTA
GCCATGATCAAATCAAGTATGCTTGCCTAAGCCTATGTATTTTACATTCT
GCATTCCTCTTAGATTCAGCTGCAACTTAATTGCTGTTTCTATTTGTTAG
GTTTCTGGTGAACCAGAGCTGTATCAAGCCTTTGTGTGATCTACTAGTCT
GCCCTGATCCTAGGATTGTTACTGTCTGTTTGGAAGGACTGGAAAACATC
TTAAAGGTAGGAGAAGCTGAGAAGAATTTGGGAAATAGTGGAGAAGTCAA
CTATTATGCTCAACTAATTGATGAAGCTGAGGGGCTTGAAAAAATAGAGA
ACCTGCAGAGTCATGACAATAACGAAATATATGAGAAGGCTGTTAAGATT
CTGGAGACATACTGGCTGGAAGAGGAAGATGAGACTCTTCCAACTGGTGA
TGGCAATCAAGCTGGATTCAACTTAGGAGGGGCTGATGGAAACCTTCCAT
CGGGAGGTTTTAATTTTGGTTAAGGTGAGCACACCTGAATTTTTTATCCT
GTATCGATCTGTTAAAAACTTCCGCAACCCCTGTTTATTAACTGATTGTT
TGCGGACTTCTGCAGGGGCTTACTTTCTGTGTGGGGAAGGTTGTCGAAGT
AAGCAAGGTCTAGTATGGCGTCGCGTCAGGTCAGGACCAGTCCGGTTGGG
TCAGGTCGGGTCAAAGCACCACATTGTGTCTTGTTGTCATCTAACACAGC
TGTTGTTGTGGCTAAAGATGAGCCGAGGTCGGTCAAGGTTTAATAGAACG
TGGTGA

Sequence Length:

7456

Sequence Checksum:

dacc326826e60d26290d02f4ea846f7a

View location in JBrowse:

DCARv2_Chr7:526441..533896-

Cds:

>DCAR_023329cds ATGTCGTTGAGACCCAACGCGAGGACCGAGGTTCGTCGGAACCGCTACAA AGTCGCCGTCGATGCCGATGAAGGTCGGCGCCGGCGAGAGGACAATATGA TCGAGATCCGGAAGAATAAGCGGGAAGAGAGCTTGCTTAAGAAGCGTCGA GAAGGCCTTCAGGCAAATCAGTTCCCTCAAGCTCCTGTCGTTGAGAAGAA GTTGCAATTGGAGAGTATTCCTGGTATGGTTGCCGGGGTGTGGTCCGATG ATAGTAATATGCAGCTGGAGGCAACCACTCACTTCCGAAAATTACTTTCA ATAGAGCGGAGCCCTCCAATCGAGGAAGTCATTCAATCTGGAGTTGTTCC ACGTTTTGTGGAGTTTCTCATGAGGGAAGACTACCCACAACTACAGGCTG TTTGGGCATTGGGAAATGTGGCTGGTGACTCTCCTCGATGTCGTGATCTT GTCCTTGCTAGTGGTGCTTTGCCGCCTTTGCTGGCTCAGTTGAATGAGCA CGCAAAACTTTCTATGCTAAGAAATGCTACCTGGACGTTATCGAATTTTT GCCGTGGCAAGCCACAACCTCCTTTTGAGCAGACTAGGCCAGCACTTCCT GCTCTCCAGCGCCTTGTTCATTCTAATGACGAAGAGGTGCTGACAGATGC ATGCTGGGCTCTATCATATCTCTCTGATGGTACTAATGATAAAATTCAAG CTGTCATCGATGCAGGTGTCTGTCAACGTCTTGTTGAGCTTCTTCTTCAT CCTTCACCATCGGTTCTTATTCCTGCACTTCGAACTGTGGGGAACATTGT GACAGGGGATGATGTCCAGACTCAGGCTGTAATTCTGGCGGGGTTATTTG GGCCTCTGGTTAACCTTCTTCAAAATGCTGAATTTGACATTAAGAAAGAG GCAGCATGGGCAATTTCAAATGCCACTTCCGGTGGTAGCCATGATCAAAT CAAGTTTCTGGTGAACCAGAGCTGTATCAAGCCTTTGTGTGATCTACTAG TCTGCCCTGATCCTAGGATTGTTACTGTCTGTTTGGAAGGACTGGAAAAC ATCTTAAAGGTAGGAGAAGCTGAGAAGAATTTGGGAAATAGTGGAGAAGT CAACTATTATGCTCAACTAATTGATGAAGCTGAGGGGCTTGAAAAAATAG AGAACCTGCAGAGTCATGACAATAACGAAATATATGAGAAGGCTGTTAAG ATTCTGGAGACATACTGGCTGGAAGAGGAAGATGAGACTCTTCCAACTGG TGATGGCAATCAAGCTGGATTCAACTTAGGAGGGGCTGATGGAAACCTTC CATCGGGAGGGGCTTACTTTCTGTGTGGGGAAGGTTGTCGAAGTAAGCAA GGTCTAGTATGGCGTCGCGTCAGGTCAGGACCAGTCCGGTTGGGTCAGGT CGGGTCAAAGCACCACATTGTGTCTTGTTGTCATCTAACACAGCTGTTGT TGTGGCTAAAGATGAGCCGAGGTCGGTCAAGGTTTAATAGAACGTGGTGA

Pep:

>DCAR_023329pep MSLRPNARTEVRRNRYKVAVDADEGRRRREDNMIEIRKNKREESLLKKRR EGLQANQFPQAPVVEKKLQLESIPGMVAGVWSDDSNMQLEATTHFRKLLS IERSPPIEEVIQSGVVPRFVEFLMREDYPQLQAVWALGNVAGDSPRCRDL VLASGALPPLLAQLNEHAKLSMLRNATWTLSNFCRGKPQPPFEQTRPALP ALQRLVHSNDEEVLTDACWALSYLSDGTNDKIQAVIDAGVCQRLVELLLH PSPSVLIPALRTVGNIVTGDDVQTQAVILAGLFGPLVNLLQNAEFDIKKE AAWAISNATSGGSHDQIKFLVNQSCIKPLCDLLVCPDPRIVTVCLEGLEN ILKVGEAEKNLGNSGEVNYYAQLIDEAEGLEKIENLQSHDNNEIYEKAVK ILETYWLEEEDETLPTGDGNQAGFNLGGADGNLPSGGAYFLCGEGCRSKQ GLVWRRVRSGPVRLGQVGSKHHIVSCCHLTQLLLWLKMSRGRSRFNRTW*