DCAR_001439 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_001439

Identifier:

DCAR_001439.gene

Locus Tag:

DCAR_001439

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 2 relationships.
Relationships
The mRNA, DCAR_001439, is a part of gene, DCAR_001439.
The genetic_marker, MK002861 K0305, is located in gene, DCAR_001439.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_001439	DCAR_001439.mRNA	mRNA	DCARv2_Chr1:15366958..15375425

Sequence:

ATGTCCTCCTCCTCCATATGGTTAGGTCTAGTAGTAGTAGTGGTGGTGTT
GTTGGTGAGCAATGCGGCGTCGTATTCTAAGTCCGAATCTTCTAATTCAG
GGAGTAGAGGACCCCACATAGCTGATGTGAATATATTGTTGCCTCCCAAG
ATGACTCATCCTGTTCACTATAGACTTCAAGCAAGTGATGGCTGCTTCAA
ATGGTATGATACCCCCAATTTTTTCTTTATTTCATCAATTTTTTTTTTTA
AATATTTAATCTCACTTGCTTACTTAACTTACAACAACATTCTACAACTT
CAATCTCAATTCTTATATGTGTTTAGCTATGTTTTACATCGGGACCGTAG
GAGTGCCATGCTTTCCTATTTAATTCACTTCTCTTTCCGCTTTCGAGTGT
AGCTAGAACACCTGCTCAACTATTGCTGCAGACTGTCTGTTTCTGTTGCA
GTGTTTAAGTAATTTTAAACTTTATATATGTATATAATGGATCAGGTCAT
GGGATCATCATGATATCTTGTCTGTTTTACCCGAGTACAATTCGAGTAAT
CACTGCTCCACGAGTGCCCACTTGAAGTCGATAGCACCCTATAGTGGGCG
CAAGGAGACGGCCGTGTATGCTACGGATGTGGATACTGGGAGGGTCATTC
GTTGCAAGGTTTATATTGATAATGTTTCTAGAATTCAAATATTTCATAGT
TCTGTCAAACTTGATTTAGACGGGCTTGCCACTTTACGTGTTCGTGCCTT
TGACAGCGAAGGTATGTCAAGCTAGTTTATGACTTAAACATTGAAACTTT
TTTTTTTCTTTCCATTGTATTGTATTAATTCATGTAATTGCAAACAACAT
TACAAACTCGATTAAGCTATACTATTTTTTTTAAGTGTCCTATGGACTAT
GGTTAGGGTCTCCGTAATGATTGATGCATCCAATTCAAACTTGTTCCTCG
TATCACGCACTTTCAACCATGACATCACTAACTGCTATCATTATACTAAA
CTTGACATGCTGATTGCTGACTTCTTATATTAATATATATAATCATGTAG
CTTTCTTGCTTCTTATGCTTTCTATGTTTTGTGCCTCTGCTGAACGCATG
ACATAATTCATTTGTAATTTGTATCATAATGCAACATTGACATGCTTACT
TGAATTGAAGTGTTAAACTGTGCGGTGTATTATTATTAATTCTTGACATG
TAAAATTGGTAAACCTTAGCTAAACCAGCATTTAACATAACAGGAGGCAG
ATTGCTGTATTTTCTGTTAAATAAAATTGAAAACGAACGGGGTCAATTGA
TATACTGATCCAATCAACCATTTATATGAATTAATCACTAAATCTTCAAT
AAAATAAAACAGTGCATTAGTAAATCTTTGATGGATATGAATATATCAGA
ACAAATCTATATTATTGCAATAATTGTTCTTGTTTCTGTGCTGCTATTTT
TCATTTGCATCATTGTATGTATAGGATGCTTAATGACTTGCATCACACTA
CTGCAGTTGAACTTTTGCTGTCAAGTGTGCAATTAGTTTACTAATTCTCA
TTTCTCTTGGCCTCTTGGGTCTATTTAGTAGAATATCAAATTTCTTATGG
ACATTTTTATCAGTTTTTGTTATTTTTCACATGGATTGAGCTAGAAATTA
AATACTGCAGGGGGCGAATTTTTTTCCTTTTAGCAAGTAATATGGAGTAA
TATTTTATTAATGTTAAAAAAACATGTTAATTATTACAAATGTCATTGTT
TACAAATATGCTTGTAGCCTTGTATTTATATTAACACCATAATACTATCG
CTATATTCATAAAATTTACATATGTTTGAGGGGGCACAAATATATACTAA
AACATTTTAGCATTAATATATATATATATATATATATATATATATATATA
TATATATATATATATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNTATATATAAATAGGCCAACATTCCAGA
GAGAGAATCCTTATGTGGAGTTACATAGTGCGCTACTATAAATTATCAAT
TTTATTATAAATTCTGTTATAGAATACATATAAAACGTGATTCTAATATA
GAATACTATAAAATAAATCTATTTTAAGTAATTTAACACTTAAAATTTGA
TTAAAAAAGTATATTTTCGAAACTGATTCTACAATAAAATAATTCTGGAT
GATTATTATTCTGTTATAGAATCATGTTGAGATATTGCATAATTTTAGAT
GATCTTTGGAATAAAAAAATTGTATAACTTCGTTTTCGGTTTAATAATCA
AAATTTGTAATTATATTTCATAAAAAATATAATAGAATATATATTATGTA
TATATTTATAATGGTGTGCTACGTAACTCCATATAAGAGGGTATGCTATG
GAGTGCTACCCTATATATATATTGCTCCTGAATTTTATACTAATTTTATG
GCTATCGAATTTTGGGCGGCAGCTGGCACCATCAGCCACCCCTTGCCTCC
ATCTTTGATTGTTATTTATATTGTTTAGACTTTAGTTGCATGTGTTCTTG
GGGCTATTATGCTTGCCGTGTCTCTGAACTGATGTATTTTTTATTTAACT
AAGATGTGAAATTATAAAAAAGCTCATATATCTTTGTTTACTGTCTCATT
GTGCAGAAAATGTGTTTTCATCCCTAGTGGGCATACGGTTCATGTGGCAA
CTCGAGCCTGAAACCGATGAATCAGCTCATCACCTTGTTCATGTACCTTT
AAAGCTCTCTCCACTAAGTGATTGTGGTGGCTTGTGCGGTGATCTCGACG
TCCAAATAAAGCTTGAAGATAGTGGAGTTTTTTCAGACCTTTATGTTGTA
AAAGGAACTGAAATCGGTCATGAACGCGTTTCAGTAGATCTGCTTGAGCC
AATGTATGATCAGATGGCTGATAGTATTGTTCTAACTGTTGCAGAAGCTA
TGTCTCTTTATCCACCATCCCCTGTTTTTGTTCTTGTTGGTGCTGTTGTG
CAATATCTACTAACAGTTGTGCGCGGGAATATTCCTCGAGCAGTAACTTT
ACCATCTCCGTATCATCAATGGTCGGTTTTGAATTCTTCAGTTTCTCGGA
TAGACAAAACGATGGGTATAGCTCATGCACTGAACTTAGGTGTCACAACT
GTTAGTGTTGAAGATACAAGAGTTGCTGGTCACATCCAACTGTCATCACT
ACATGTAGTGCTTCCAGATTCTTTATATATATACATATCGCCTTTGTCAC
ATTCTGGTGATATTCTCGAAGGTGCAGCACCTAATCCATCTGTTTCACGT
TGGTATGTTGTTACTGGTAGACAATATCTTCTTGAAATGAAAGTTTTCTC
GCGAGTACCTGAGTCACAAGAGATATATATAACGGAGACTGATGATATAA
AGTTGTATGATGACCATTCTGAGTTTTGGAATATATTCCCAGTTTCAGAT
ACTACTACTTCCAAACTTGTGTGTTTGAATTCTAAAATTCTGAAAGCAAT
GTCATATGGATTGGGTAAACTAACTGCAACTTTAACCTACAGTAATGGGG
TTCATGAGGTGAATCAGGCTCTCAAGGTTGTGCAAGAAATCATGGTTTGT
GAGCAAGTAAAGTTTAGTAAGGACAAAAGAAGTGCATCTGGGAGTATTCT
CCTTCCATGGGCCCCTTCTGTCCACCAGGAGGTGGAGCTAAAGGCAACAG
GAGGTTGTGCCATATCATCCAGTGACTACAAATGGTTTTCTTCAGATATG
TCCATTGTGTGTGTATCAGCACATGGTATTGTTCAGTCAAAAAGACCAGG
GAAAGTTACTATTAAAGTGGTATCTAGTTTTGATTCTTTCAATTACGATG
AGTTGGAAATTGAAGTTTCCATTCCTTCCTCTATGATCATGCTGCAACAA
TTTCCTGTAGAGACTGTTGTGGGATCAAATCTTGCAGCTTCCGTGACACT
TAAAGATTCTAATGGTGATAATTATTTCAGATGTGATGCTTTCAGTTCAG
CCATACAGTGGAATACCGGAAGCGATTCTTTTACGATTGTCTATACAGAG
AGAGAATCATTCTTATTTGATGAGCAAGACGTCCCTGATTTGCTTGCATC
ATTATATAGCCCGCCATGTGCCTGGGCCCGTATATATGCTTCTGCTACTG
GGCGAACAATGCTACATGCTTCACTTAAAAAGGATTATCATCCTCTTGAT
CATTCGCTCACCGGATCTATCGATTTAAAAGCATCAGCTCTCATTTCAGC
ATATCTACCACTCTTCGTGCATCAGGCAGGTGATGGGAACCAGTATGGTG
GTTATTGGTTTAATTTGTCTTATGCTGAGGCTAATAACCATTTAGAGAAT
TTGAAAATCTTGAATCTTGTTCCTGGCACACATATGGATGTCACTCTGTC
CGGGGGACCAGATCAGTGGGGAGGAGGTGTTGAATTCACTGAAACTGTGG
AAATTTTACCTCAGGAACAGACTAAGCAGAAAGGGGTTTTGGTCCATAAT
ATAACTACCATTTATGGTAGCTCATATAGAATATCATGCGAGAATTGGGG
AGAATTTAAAGTTCTTTTCAAACGTGGAAACTTGGCTGGCGATGATCATA
TACTACCTGCTGTGGCGGAGGCTGAACTATTGCTCTCATGTATCTTACCA
TCTTCAATCTCAATAGTAGTTGATAGTTCTGTCAATGCCCTTGATGTGAT
ACACACAGCTATGCAGGCTGACCGGAATTCAGAGCAAGTTCGGGCCCTTC
CAATCACTGTGGCTAATGGGCGAACTATTAGAGTATCTGCTGTTGGTATT
AGTGACTCTGGGAATGCTTTTGGGAACTCTTCTTCTCTTAGTATAAGTTG
GGAAGCGAGAAATTGTGATGGATTGGCCTTCTGGGATGCTGCTGCTACAT
CCATATCAAATTGGGAGAGGTTTTTGGTCTTGCAAAACTCAACAGGACTT
TGCACTGTTCGTGCCACAGTTGGTGGATTCATAGAGACCTTGAGTAATCA
TCGCTTTATTAAATCCTATGGAAGTTCAAAATCTCTTACCGATGCCGTAC
AACTACAACTTGTCTCTACTTTAAAAGTCACTCCAGAGTTCAGCTTGTTA
TTATTCAGTCACTTGGCTATATTAGATCTCTCAATTAGTGGAGGGAGTTG
TGCACTGACTACTGTGGTTAATGATACCCAAGTTGCAGAATTAATTGATC
GTACACCAGATTTGCATTGCTCTCAACTATCACTAGCGCCTAAAAGTTTA
GGGACTGCACTTGTTACGGTTTATGATATGGGACTTTCTCCCCCACTTGC
TGCCACCTCCGTGGTTCAAGTTGCTGATGTAGATTGGCTTAAGATTACTT
CGGGAGAACTAATAAGCCTCGTGGAAGGAAGTTCCCAATCTATAGATCTT
CTGGCTGGGGTAAATAATGGACGCACTTTTGACTTCTCCCAGTACATGTA
TATGAAAATTCGTGTGCATATTGAAGATCATAATATTGTTAGTCTGTTAG
ATGGTATTAGCGATGGATATCTGAAGGCTCCAAAATTTATCCTTCAGGCC
AAAAATCACGGTATTACGACTCTTTATGTAAGTGCTAGGCAACAATCTGG
ACGTGAAATATTGAGCCAATTTATTAAGGTTGAAGTTTATGCCCCACTTA
GTATACGTCCTTCTAATATATATCTTGTACCCGGTGCTTCTTATGTGCTT
ACCGTGGAAGGAGGCCCAACTATTGGTGGTTATGTTGAATATGGAAGTAT
GAATGATGAGACTGCAATAATTCATAAATCTTCTGGGCGGCTGATTGCAA
TTGCTCCAGGGAATACAACTCTCGTGGCTAGAATTTATAGCAAAGGTGAT
GAAGTGATGTGTCATGCATTTGGATATGTAGTAGTTGAGATCCCTTCTTC
AGCAATGTTAAATGTGCAGAGTGAGCAACTTGCCATTGGTAGCGAACTAC
AAATATATCCTTCGTTTACCAACGGTAATTTGTTTTCTTTGTATGAGGTG
TGCAAGAATTATGAATGGAGCGTTGAAGATGAAGACGTATTGACTTTCAA
AGTAGTAGATCTCTTGCATGGTTACAAGCTTGGTGCCTCTTCCATTGTTT
TGGAAGCAAGTGGATCTAATGAGAAGCTTGACCTAAGGTTTATCCGCGAG
TTTTATGGAAAGTCTTCTGGCCAGACTCATGTCAGCGTTTCATTTTCTTG
TAATTTTGTATCCCGTTCATTTTCACAGTCCAGGTTATATACTGCATCCA
TATTGCTCTCTGTGGTGCCTGATCTCCCTCTTGCTCTTGGAAAACCAGTG
ACATGGGTACTTCCTCCTCATTATATAACATCAAATCTCTTACCATCATC
TTCAGGGTCCTACAGTCAGGGAGATGCTCAGAGCAGCAAAGGAACCATTA
CTTACACTTTGTTAAAAGAATATGGGGGTCAGAAAGATATAGAGTTGGTA
AATGAAGCAATATCCATCCAAGGTGTTAAAATACAAACAAGAGAGAGTAA
TAATATTGGGTGCATTCAGGCGAAAGATAGATTGACTGGAAGATTTGAGA
TTGCATCTTGTGTTAGAGTCGCTGAGGTAGCTCAAATCAGAGTGAGCAGG
CATGCGTTTCCATTTTACAGCGTTAACCTTGCTGTTGGAGCTGAACTCAG
TATTCCGCTGATATATTACGATGCTTTTGGATACCCTTTCTACGAGGCTT
ATAACATTGTTTTGTATGATGTTGAGACTAACTATGGCGACATTGTCTCC
ATCAATGACACACATGACGGTAATATATATCTGAAAGCATTACAATTTGG
TAGAGCACTGGTCCGAATATCCTTAAAAAATAACTCAACCAAGTCGGACT
ACTTGTTGATCACAATTGGTGCTCGTTTGTACCCTCATAATCCACTCCTC
CAATTGGGTAATCGTCTTAATTTCAGTATTGAAGGCCTCACTGATCAAGT
ATCTGGCTGGTGGACGAGTGTTAATACAAGTACTGTTTCAATAGACAGGG
TGTCTGGAGAAGCTGAAGCCATTGGGGAAGGAAGTACTCAAGTCATCTAT
GAAAGCTCAGATTTCAAGTTGCTGACGTTTGTTACAGTCTCAAAGGGGCA
TATGATAACTGTTAATGCTCCAAAAGGGATTCTAACAAATGCTCCTTTCT
CAATGAGAGGTTATAAATTCCTTGTGACATTTAGTGATGCGTTTGAACCC
ATCCGTGAACCAGGGGGGGCTGTTAAAACACTCCTGCATGAGTGTGAAGT
GGAGCCACATTTTGTTGGGTATGCAGACCCATGGATGGATTTTGAGAGTG
GAATGTCATATTGTCTCTTCTTTCCATACTCACCAGGACACTTGGTTCGT
TCAGTTGGAAATTTGAAGGATACAAGGCGGGATATAAGTGTTACCATCAA
TGCTTTTGTGCGAGGAACAAACAATATATCTGGATCTGCTTCTGCTTTTT
TTGTTGGTGGATTTGCTATACTGGACATGGACCACAACTCATTACATTTG
AATTTGACTTCCAAGTCTAACAAGTCTGTCATAACTGTAGTCGGTAATAC
AGATGTTGTTGTTGACTCGCAAGGTCGGAATCAGTTGCTAATCACACCTA
TACACAGAGAAAATAATGGAGTAGCAGGGCGGGCAGAATACGAGGTCAAA
GTTCTCAGCTCTTGGAGGTTCAAAGATAAATTGATAATTTCACTCGCAGC
AAATGGCCAGAGAGTCGAAATTGATGTAAACTATGAACCTGAGAAGATAC
CCGCACTGCCTTCATCCACTAAAGTCAGCAACTGGATGGTTATACTTGTG
TGTTCCTCTGTTCTAATATCAACACTAATTCTGTTTCTCTGTTACCTGGA
TAGATCAAATAGATATCAAGCAAATGCGGCACCTACCACCCGAACTGCCA
CAGTTCAATCACCGGCAACCCCTCCTCGGAGCAGTCCATCCGTCGTGAAT
GAACACTCTCCACAAACGCCTCAAGCTTTCATAGACTATGTAAGGAGAAC
AGTTGATGAGACTCCTTACTACAGGAAAGATCCGCGCAGGAGGTTTAATG
CACAAAATACCTTTTAG

Sequence Length:

8467

Sequence Checksum:

1a129afd56a1d1dde0860ef72dbd4381

View location in JBrowse:

DCARv2_Chr1:15366959..15375425+

Cds:

>DCAR_001439cds ATGTCCTCCTCCTCCATATGGTTAGGTCTAGTAGTAGTAGTGGTGGTGTT GTTGGTGAGCAATGCGGCGTCGTATTCTAAGTCCGAATCTTCTAATTCAG GGAGTAGAGGACCCCACATAGCTGATGTGAATATATTGTTGCCTCCCAAG ATGACTCATCCTGTTCACTATAGACTTCAAGCAAGTGATGGCTGCTTCAA ATGGTCATGGGATCATCATGATATCTTGTCTGTTTTACCCGAGTACAATT CGAGTAATCACTGCTCCACGAGTGCCCACTTGAAGTCGATAGCACCCTAT AGTGGGCGCAAGGAGACGGCCGTGTATGCTACGGATGTGGATACTGGGAG GGTCATTCGTTGCAAGGTTTATATTGATAATGTTTCTAGAATTCAAATAT TTCATAGTTCTGTCAAACTTGATTTAGACGGGCTTGCCACTTTACGTGTT CGTGCCTTTGACAGCGAAGAAAATGTGTTTTCATCCCTAGTGGGCATACG GTTCATGTGGCAACTCGAGCCTGAAACCGATGAATCAGCTCATCACCTTG TTCATGTACCTTTAAAGCTCTCTCCACTAAGTGATTGTGGTGGCTTGTGC GGTGATCTCGACGTCCAAATAAAGCTTGAAGATAGTGGAGTTTTTTCAGA CCTTTATGTTGTAAAAGGAACTGAAATCGGTCATGAACGCGTTTCAGTAG ATCTGCTTGAGCCAATGTATGATCAGATGGCTGATAGTATTGTTCTAACT GTTGCAGAAGCTATGTCTCTTTATCCACCATCCCCTGTTTTTGTTCTTGT TGGTGCTGTTGTGCAATATCTACTAACAGTTGTGCGCGGGAATATTCCTC GAGCAGTAACTTTACCATCTCCGTATCATCAATGGTCGGTTTTGAATTCT TCAGTTTCTCGGATAGACAAAACGATGGGTATAGCTCATGCACTGAACTT AGGTGTCACAACTGTTAGTGTTGAAGATACAAGAGTTGCTGGTCACATCC AACTGTCATCACTACATGTAGTGCTTCCAGATTCTTTATATATATACATA TCGCCTTTGTCACATTCTGGTGATATTCTCGAAGGTGCAGCACCTAATCC ATCTGTTTCACGTTGGTATGTTGTTACTGGTAGACAATATCTTCTTGAAA TGAAAGTTTTCTCGCGAGTACCTGAGTCACAAGAGATATATATAACGGAG ACTGATGATATAAAGTTGTATGATGACCATTCTGAGTTTTGGAATATATT CCCAGTTTCAGATACTACTACTTCCAAACTTGTGTGTTTGAATTCTAAAA TTCTGAAAGCAATGTCATATGGATTGGGTAAACTAACTGCAACTTTAACC TACAGTAATGGGGTTCATGAGGTGAATCAGGCTCTCAAGGTTGTGCAAGA AATCATGGTTTGTGAGCAAGTAAAGTTTAGTAAGGACAAAAGAAGTGCAT CTGGGAGTATTCTCCTTCCATGGGCCCCTTCTGTCCACCAGGAGGTGGAG CTAAAGGCAACAGGAGGTTGTGCCATATCATCCAGTGACTACAAATGGTT TTCTTCAGATATGTCCATTGTGTGTGTATCAGCACATGGTATTGTTCAGT CAAAAAGACCAGGGAAAGTTACTATTAAAGTGGTATCTAGTTTTGATTCT TTCAATTACGATGAGTTGGAAATTGAAGTTTCCATTCCTTCCTCTATGAT CATGCTGCAACAATTTCCTGTAGAGACTGTTGTGGGATCAAATCTTGCAG CTTCCGTGACACTTAAAGATTCTAATGGTGATAATTATTTCAGATGTGAT GCTTTCAGTTCAGCCATACAGTGGAATACCGGAAGCGATTCTTTTACGAT TGTCTATACAGAGAGAGAATCATTCTTATTTGATGAGCAAGACGTCCCTG ATTTGCTTGCATCATTATATAGCCCGCCATGTGCCTGGGCCCGTATATAT GCTTCTGCTACTGGGCGAACAATGCTACATGCTTCACTTAAAAAGGATTA TCATCCTCTTGATCATTCGCTCACCGGATCTATCGATTTAAAAGCATCAG CTCTCATTTCAGCATATCTACCACTCTTCGTGCATCAGGCAGGTGATGGG AACCAGTATGGTGGTTATTGGTTTAATTTGTCTTATGCTGAGGCTAATAA CCATTTAGAGAATTTGAAAATCTTGAATCTTGTTCCTGGCACACATATGG ATGTCACTCTGTCCGGGGGACCAGATCAGTGGGGAGGAGGTGTTGAATTC ACTGAAACTGTGGAAATTTTACCTCAGGAACAGACTAAGCAGAAAGGGGT TTTGGTCCATAATATAACTACCATTTATGGTAGCTCATATAGAATATCAT GCGAGAATTGGGGAGAATTTAAAGTTCTTTTCAAACGTGGAAACTTGGCT GGCGATGATCATATACTACCTGCTGTGGCGGAGGCTGAACTATTGCTCTC ATGTATCTTACCATCTTCAATCTCAATAGTAGTTGATAGTTCTGTCAATG CCCTTGATGTGATACACACAGCTATGCAGGCTGACCGGAATTCAGAGCAA GTTCGGGCCCTTCCAATCACTGTGGCTAATGGGCGAACTATTAGAGTATC TGCTGTTGGTATTAGTGACTCTGGGAATGCTTTTGGGAACTCTTCTTCTC TTAGTATAAGTTGGGAAGCGAGAAATTGTGATGGATTGGCCTTCTGGGAT GCTGCTGCTACATCCATATCAAATTGGGAGAGGTTTTTGGTCTTGCAAAA CTCAACAGGACTTTGCACTGTTCGTGCCACAGTTGGTGGATTCATAGAGA CCTTGAGTAATCATCGCTTTATTAAATCCTATGGAAGTTCAAAATCTCTT ACCGATGCCGTACAACTACAACTTGTCTCTACTTTAAAAGTCACTCCAGA GTTCAGCTTGTTATTATTCAGTCACTTGGCTATATTAGATCTCTCAATTA GTGGAGGGAGTTGTGCACTGACTACTGTGGTTAATGATACCCAAGTTGCA GAATTAATTGATCGTACACCAGATTTGCATTGCTCTCAACTATCACTAGC GCCTAAAAGTTTAGGGACTGCACTTGTTACGGTTTATGATATGGGACTTT CTCCCCCACTTGCTGCCACCTCCGTGGTTCAAGTTGCTGATGTAGATTGG CTTAAGATTACTTCGGGAGAACTAATAAGCCTCGTGGAAGGAAGTTCCCA ATCTATAGATCTTCTGGCTGGGGTAAATAATGGACGCACTTTTGACTTCT CCCAGTACATGTATATGAAAATTCGTGTGCATATTGAAGATCATAATATT GTTAGTCTGTTAGATGGTATTAGCGATGGATATCTGAAGGCTCCAAAATT TATCCTTCAGGCCAAAAATCACGGTATTACGACTCTTTATGTAAGTGCTA GGCAACAATCTGGACGTGAAATATTGAGCCAATTTATTAAGGTTGAAGTT TATGCCCCACTTAGTATACGTCCTTCTAATATATATCTTGTACCCGGTGC TTCTTATGTGCTTACCGTGGAAGGAGGCCCAACTATTGGTGGTTATGTTG AATATGGAAGTATGAATGATGAGACTGCAATAATTCATAAATCTTCTGGG CGGCTGATTGCAATTGCTCCAGGGAATACAACTCTCGTGGCTAGAATTTA TAGCAAAGGTGATGAAGTGATGTGTCATGCATTTGGATATGTAGTAGTTG AGATCCCTTCTTCAGCAATGTTAAATGTGCAGAGTGAGCAACTTGCCATT GGTAGCGAACTACAAATATATCCTTCGTTTACCAACGGTAATTTGTTTTC TTTGTATGAGGTGTGCAAGAATTATGAATGGAGCGTTGAAGATGAAGACG TATTGACTTTCAAAGTAGTAGATCTCTTGCATGGTTACAAGCTTGGTGCC TCTTCCATTGTTTTGGAAGCAAGTGGATCTAATGAGAAGCTTGACCTAAG GTTTATCCGCGAGTTTTATGGAAAGTCTTCTGGCCAGACTCATGTCAGCG TTTCATTTTCTTGTAATTTTGTATCCCGTTCATTTTCACAGTCCAGGTTA TATACTGCATCCATATTGCTCTCTGTGGTGCCTGATCTCCCTCTTGCTCT TGGAAAACCAGTGACATGGGTACTTCCTCCTCATTATATAACATCAAATC TCTTACCATCATCTTCAGGGTCCTACAGTCAGGGAGATGCTCAGAGCAGC AAAGGAACCATTACTTACACTTTGTTAAAAGAATATGGGGGTCAGAAAGA TATAGAGTTGGTAAATGAAGCAATATCCATCCAAGGTGTTAAAATACAAA CAAGAGAGAGTAATAATATTGGGTGCATTCAGGCGAAAGATAGATTGACT GGAAGATTTGAGATTGCATCTTGTGTTAGAGTCGCTGAGGTAGCTCAAAT CAGAGTGAGCAGGCATGCGTTTCCATTTTACAGCGTTAACCTTGCTGTTG GAGCTGAACTCAGTATTCCGCTGATATATTACGATGCTTTTGGATACCCT TTCTACGAGGCTTATAACATTGTTTTGTATGATGTTGAGACTAACTATGG CGACATTGTCTCCATCAATGACACACATGACGGTAATATATATCTGAAAG CATTACAATTTGGTAGAGCACTGGTCCGAATATCCTTAAAAAATAACTCA ACCAAGTCGGACTACTTGTTGATCACAATTGGTGCTCGTTTGTACCCTCA TAATCCACTCCTCCAATTGGGTAATCGTCTTAATTTCAGTATTGAAGGCC TCACTGATCAAGTATCTGGCTGGTGGACGAGTGTTAATACAAGTACTGTT TCAATAGACAGGGTGTCTGGAGAAGCTGAAGCCATTGGGGAAGGAAGTAC TCAAGTCATCTATGAAAGCTCAGATTTCAAGTTGCTGACGTTTGTTACAG TCTCAAAGGGGCATATGATAACTGTTAATGCTCCAAAAGGGATTCTAACA AATGCTCCTTTCTCAATGAGAGGTTATAAATTCCTTGTGACATTTAGTGA TGCGTTTGAACCCATCCGTGAACCAGGGGGGGCTGTTAAAACACTCCTGC ATGAGTGTGAAGTGGAGCCACATTTTGTTGGGTATGCAGACCCATGGATG GATTTTGAGAGTGGAATGTCATATTGTCTCTTCTTTCCATACTCACCAGG ACACTTGGTTCGTTCAGTTGGAAATTTGAAGGATACAAGGCGGGATATAA GTGTTACCATCAATGCTTTTGTGCGAGGAACAAACAATATATCTGGATCT GCTTCTGCTTTTTTTGTTGGTGGATTTGCTATACTGGACATGGACCACAA CTCATTACATTTGAATTTGACTTCCAAGTCTAACAAGTCTGTCATAACTG TAGTCGGTAATACAGATGTTGTTGTTGACTCGCAAGGTCGGAATCAGTTG CTAATCACACCTATACACAGAGAAAATAATGGAGTAGCAGGGCGGGCAGA ATACGAGGTCAAAGTTCTCAGCTCTTGGAGGTTCAAAGATAAATTGATAA TTTCACTCGCAGCAAATGGCCAGAGAGTCGAAATTGATGTAAACTATGAA CCTGAGAAGATACCCGCACTGCCTTCATCCACTAAAGTCAGCAACTGGAT GGTTATACTTGTGTGTTCCTCTGTTCTAATATCAACACTAATTCTGTTTC TCTGTTACCTGGATAGATCAAATAGATATCAAGCAAATGCGGCACCTACC ACCCGAACTGCCACAGTTCAATCACCGGCAACCCCTCCTCGGAGCAGTCC ATCCGTCGTGAATGAACACTCTCCACAAACGCCTCAAGCTTTCATAGACT ATGTAAGGAGAACAGTTGATGAGACTCCTTACTACAGGAAAGATCCGCGC AGGAGGTTTAATGCACAAAATACCTTTTAG

Pep:

>DCAR_001439pep MSSSSIWLGLVVVVVVLLVSNAASYSKSESSNSGSRGPHIADVNILLPPK MTHPVHYRLQASDGCFKWSWDHHDILSVLPEYNSSNHCSTSAHLKSIAPY SGRKETAVYATDVDTGRVIRCKVYIDNVSRIQIFHSSVKLDLDGLATLRV RAFDSEENVFSSLVGIRFMWQLEPETDESAHHLVHVPLKLSPLSDCGGLC GDLDVQIKLEDSGVFSDLYVVKGTEIGHERVSVDLLEPMYDQMADSIVLT VAEAMSLYPPSPVFVLVGAVVQYLLTVVRGNIPRAVTLPSPYHQWSVLNS SVSRIDKTMGIAHALNLGVTTVSVEDTRVAGHIQLSSLHVVLPDSLYIYI SPLSHSGDILEGAAPNPSVSRWYVVTGRQYLLEMKVFSRVPESQEIYITE TDDIKLYDDHSEFWNIFPVSDTTTSKLVCLNSKILKAMSYGLGKLTATLT YSNGVHEVNQALKVVQEIMVCEQVKFSKDKRSASGSILLPWAPSVHQEVE LKATGGCAISSSDYKWFSSDMSIVCVSAHGIVQSKRPGKVTIKVVSSFDS FNYDELEIEVSIPSSMIMLQQFPVETVVGSNLAASVTLKDSNGDNYFRCD AFSSAIQWNTGSDSFTIVYTERESFLFDEQDVPDLLASLYSPPCAWARIY ASATGRTMLHASLKKDYHPLDHSLTGSIDLKASALISAYLPLFVHQAGDG NQYGGYWFNLSYAEANNHLENLKILNLVPGTHMDVTLSGGPDQWGGGVEF TETVEILPQEQTKQKGVLVHNITTIYGSSYRISCENWGEFKVLFKRGNLA GDDHILPAVAEAELLLSCILPSSISIVVDSSVNALDVIHTAMQADRNSEQ VRALPITVANGRTIRVSAVGISDSGNAFGNSSSLSISWEARNCDGLAFWD AAATSISNWERFLVLQNSTGLCTVRATVGGFIETLSNHRFIKSYGSSKSL TDAVQLQLVSTLKVTPEFSLLLFSHLAILDLSISGGSCALTTVVNDTQVA ELIDRTPDLHCSQLSLAPKSLGTALVTVYDMGLSPPLAATSVVQVADVDW LKITSGELISLVEGSSQSIDLLAGVNNGRTFDFSQYMYMKIRVHIEDHNI VSLLDGISDGYLKAPKFILQAKNHGITTLYVSARQQSGREILSQFIKVEV YAPLSIRPSNIYLVPGASYVLTVEGGPTIGGYVEYGSMNDETAIIHKSSG RLIAIAPGNTTLVARIYSKGDEVMCHAFGYVVVEIPSSAMLNVQSEQLAI GSELQIYPSFTNGNLFSLYEVCKNYEWSVEDEDVLTFKVVDLLHGYKLGA SSIVLEASGSNEKLDLRFIREFYGKSSGQTHVSVSFSCNFVSRSFSQSRL YTASILLSVVPDLPLALGKPVTWVLPPHYITSNLLPSSSGSYSQGDAQSS KGTITYTLLKEYGGQKDIELVNEAISIQGVKIQTRESNNIGCIQAKDRLT GRFEIASCVRVAEVAQIRVSRHAFPFYSVNLAVGAELSIPLIYYDAFGYP FYEAYNIVLYDVETNYGDIVSINDTHDGNIYLKALQFGRALVRISLKNNS TKSDYLLITIGARLYPHNPLLQLGNRLNFSIEGLTDQVSGWWTSVNTSTV SIDRVSGEAEAIGEGSTQVIYESSDFKLLTFVTVSKGHMITVNAPKGILT NAPFSMRGYKFLVTFSDAFEPIREPGGAVKTLLHECEVEPHFVGYADPWM DFESGMSYCLFFPYSPGHLVRSVGNLKDTRRDISVTINAFVRGTNNISGS ASAFFVGGFAILDMDHNSLHLNLTSKSNKSVITVVGNTDVVVDSQGRNQL LITPIHRENNGVAGRAEYEVKVLSSWRFKDKLIISLAANGQRVEIDVNYE PEKIPALPSSTKVSNWMVILVCSSVLISTLILFLCYLDRSNRYQANAAPT TRTATVQSPATPPRSSPSVVNEHSPQTPQAFIDYVRRTVDETPYYRKDPR RRFNAQNTF*