MSSPPPPPLPDSQKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMP
IICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTV
SVSLRNSNNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEE
KSDEVSEHSVGPAGGFWALPGRPDYGQVWSFAAGSDMVVPTPVNMSQQNR
YMQVQGEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREEDHH*
| Relationships |
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| The polypeptide, DCAR_002594, derives from mRNA, DCAR_002594. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A2J6K9I8 (TCP domain-containing protein {ECO:0000259|PROSITE:PS51369})
HSP 1 Score: 318.546 bits (815), Expect = 6.654e-108
Identity = 172/244 (70.49%), Postives = 195/244 (79.92%), Query Frame = 0
Query: 11 DSQKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNSNN-SLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVGPA---GGFWALPGRPDYGQVWSFAAG-SDMVVPTPVNM-SQQNRYMQVQ---GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
D+Q+Q P NG +AVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSV++RNSNN SLSA L+QK + Q +QHL +PTPFILGKR R E+ G K D +S +V A GGFWALP RPD+GQVWSFAA +MVV +P + SQQ R++ Q GEASAARVGNYLP+TQGHLNLLASLSG QS GR+++D
Sbjct: 18 DTQRQ---QPPGNGVLAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVAVRNSNNSSLSAALDQKSSSQHHQHLISPTPFILGKRLRSEEDGLDGGGKEDNMSSATVVSAAAGGGFWALPARPDFGQVWSFAAAPQEMVVSSPTALASQQGRFIHNQQPMGEASAARVGNYLPMTQGHLNLLASLSGPPPQS-SGRKDDD 257
Match: A0A6S7ML55 (TCP domain-containing protein {ECO:0000259|PROSITE:PS51369})
HSP 1 Score: 318.546 bits (815), Expect = 6.654e-108
Identity = 172/244 (70.49%), Postives = 195/244 (79.92%), Query Frame = 0
Query: 11 DSQKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNSNN-SLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVGPA---GGFWALPGRPDYGQVWSFAAG-SDMVVPTPVNM-SQQNRYMQVQ---GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
D+Q+Q P NG +AVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSV++RNSNN SLSA L+QK + Q +QHL +PTPFILGKR R E+ G K D +S +V A GGFWALP RPD+GQVWSFAA +MVV +P + SQQ R++ Q GEASAARVGNYLP+TQGHLNLLASLSG QS GR+++D
Sbjct: 18 DTQRQ---QPPGNGVLAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVAVRNSNNSSLSAALDQKSSSQHHQHLISPTPFILGKRLRSEEDGLDGGGKEDNMSSATVVSAAAGGGFWALPARPDFGQVWSFAAAPQEMVVSSPTALASQQGRFIHNQQPMGEASAARVGNYLPMTQGHLNLLASLSGPPPQS-SGRKDDD 257
Match: A0A103XS39 ((Transcription factor, TCP {ECO:0000313|EMBL:KVH95851.1}))
HSP 1 Score: 313.923 bits (803), Expect = 3.994e-106
Identity = 168/236 (71.19%), Postives = 187/236 (79.24%), Query Frame = 0
Query: 21 PTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNS--NNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVGPA---GGFWALPGRPDYGQVWSFAAG-SDMVVPTPVNM-SQQNRYM-----QVQGEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREE 244
P NG ++VKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSV++RNS N+SLSA L+QK Q +QHL +PTPFILGKR R E+ G K D +S +V A GGFWALP R D+GQVWSFAA +MVV +P M SQQ RY+ Q GEASAARVGNYLPITQGHLNLLASLSG QS GG+ ++
Sbjct: 25 PGNGVLSVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVAVRNSNANSSLSAALDQKSTSQHHQHLISPTPFILGKRLRSEEDGLDGGGKEDNMSSATVVSAAAGGGFWALPARTDFGQVWSFAAAPPEMVVSSPSAMASQQGRYIHNHNQQPMGEASAARVGNYLPITQGHLNLLASLSGPPPQSSGGKDDD 260
Match: A0A2R6PGI9 ((Transcription factor like {ECO:0000313|EMBL:PSR90998.1}))
HSP 1 Score: 298.901 bits (764), Expect = 2.000e-100
Identity = 164/233 (70.39%), Postives = 181/233 (77.68%), Query Frame = 0
Query: 21 PTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNS---NNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVGPAGGFWALPGRPDYGQVWSFAAG-SDMVVPTPVNMSQ---QNRYMQVQ--GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREE 244
P NG + VKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSVSLRN+ ++S+SA L+ K + TPTPFILGKR R +D G K D+VS VGPAGGFWALP RPD+GQVWSFAA DMV+P+P SQ R++Q Q GEASAARVGNYLPI QGHLNLLASLSG+ S R +E
Sbjct: 21 PPNGALVVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVSLRNNSSSHSSISAALDHKP-------MITPTPFILGKRFRPDDDTVDGAAK-DDVSAAVVGPAGGFWALPARPDFGQVWSFAAAPPDMVMPSPAMTSQPPFTGRFIQQQPMGEASAARVGNYLPIAQGHLNLLASLSGAPAASSSRREDE 245
Match: A0A251SKI8 ((Putative TCP family transcription factor {ECO:0000313|EMBL:OTF99233.1}))
HSP 1 Score: 297.36 bits (760), Expect = 1.092e-99
Identity = 165/244 (67.62%), Postives = 186/244 (76.23%), Query Frame = 0
Query: 11 DSQKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNS--NNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVG--PAGGFWALPGRPDYGQVWSFAAG---SDMVVPTPVNMSQQNRYMQVQ--GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
++Q+QL NG ++VKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSV++RNS N+SLS L+QK Q +QHL +P PFILGKR R ED G K D SV GGFWALPGRPDYGQVWSFAA +V P+ V M+ Q R++Q Q GEASAARVGNYLPIT GHLNLLASLSG QS + +++
Sbjct: 18 NNQQQLPQG---NGVLSVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVAVRNSNANSSLSVSLDQKSPSQHHQHLISPAPFILGKRLRPEDDGLDGGGKEDNSHNISVSGGGGGGFWALPGRPDYGQVWSFAAAPMPETVVSPSNV-MTSQGRFIQQQPMGEASAARVGNYLPITHGHLNLLASLSGPPPQSSNNKDDDN 257
Match: A0A2J6K5H6 (TCP domain-containing protein {ECO:0000259|PROSITE:PS51369})
HSP 1 Score: 291.967 bits (746), Expect = 8.386e-98
Identity = 162/231 (70.13%), Postives = 182/231 (78.79%), Query Frame = 0
Query: 23 NGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNS--NNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVGPA--GGFWALPGRPDYGQVWSFAAGSDMVVPTPVNM-SQQNRYM---QVQGEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
NG + VKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGT PA+FS+VSV+LRNS N SLSA L+QK +QHLF+PTPFILGKR R + G ++ +S +V A GGFWALP R D+GQVWSFAA +MVVP+P+ M SQQ R++ Q GEASAARVGNYLP+TQGHLNLLASLS GRRE+D
Sbjct: 12 NGALVVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTNPASFSSVSVALRNSNANCSLSAALDQKPP---SQHLFSPTPFILGKRLRANEDGLDGHKEDHNMSSGTVTTATPGGFWALPARSDFGQVWSFAA-PEMVVPSPLAMSSQQGRFIHQEQTMGEASAARVGNYLPMTQGHLNLLASLS-GPPPPSSGRREDD 237
Match: A0A251RML5 ((Putative TCP family transcription factor {ECO:0000313|EMBL:OTF85576.1}))
HSP 1 Score: 290.812 bits (743), Expect = 1.687e-96
Identity = 157/228 (68.86%), Postives = 173/228 (75.88%), Query Frame = 0
Query: 25 TVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNS--NNSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSDEVSEHSVG--PAGGFWALPGRPDYGQVWSFAAG---SDMVVPTPVNMSQQNRYMQVQGEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
++VKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPA+FSTVSV++RNS N+SLS L+QK Q +QHL +P PFILGKR R ED G K D SV GGFWALPGRPD+GQVWSFAA +V P+ V SQ Q GEASAARVGNYLPIT GHLNLLASLSG QS + +++
Sbjct: 72 VLSVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSTVSVAVRNSHANSSLSVSLDQKSPSQHHQHLISPAPFILGKRLRPEDDGLDGGGKEDNSHNISVSGGGGGGFWALPGRPDFGQVWSFAAAPMPETVVSPSNVMTSQGRFIQQPMGEASAARVGNYLPITHGHLNLLASLSGPPPQSSNNKDDDN 299
Match: A0A0V0HYT0 ((Putative transcription factor TCP7-like {ECO:0000313|EMBL:JAP25396.1}))
HSP 1 Score: 289.656 bits (740), Expect = 2.026e-96
Identity = 159/244 (65.16%), Postives = 181/244 (74.18%), Query Frame = 0
Query: 22 TNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNSN----NSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSD----EVSEHSVGPAGGFWALPGRPDYGQVWSFAAG--SDMVVPTPVNM---------SQQNRYMQVQ-GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
TNG + VKKPP KDRHSKVDGRGRRIRMPI+CAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGT PA+FSTVSVS+RNS +SLSAPL+QK + H+ +P PFILGKR R +D K D + +VGP GFWA+P RPD+GQ+WSFAAG +M+VPT SQ +R+ Q Q GEASAARVGNYLP+TQGHLNLLASLSG Q S GRR++D
Sbjct: 28 TNGALTVKKPPVKDRHSKVDGRGRRIRMPIVCAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTIPASFSTVSVSVRNSTASLVSSLSAPLDQKSS-----HMISPAPFILGKRLRSDDENLENGNKDDVAVAAGAATAVGPTAGFWAVPARPDFGQIWSFAAGPPPEMMVPTSAAAAAAAAAALSSQSSRFFQQQMGEASAARVGNYLPMTQGHLNLLASLSGPPQPS-SGRRDDD 265
Match: G3BGV4 ((TCP transcription factor 14 {ECO:0000313|EMBL:ADM87263.1}))
HSP 1 Score: 288.5 bits (737), Expect = 5.592e-96
Identity = 160/253 (63.24%), Postives = 182/253 (71.94%), Query Frame = 0
Query: 13 QKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNSN----NSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSD----EVSEHSVGPAGGFWALPGRPDYGQVWSFAAG--SDMVVPTPVNM---------SQQNRYMQVQ-GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
Q+Q T TNG + VKKPP KDRHSKVDGRGRRIRMPI+CAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGT PA+FSTVSVS+RNS +SLSAPL+QK + + +P PFILGKR R +D K D +VGP GFWA+P RPD+GQ+WSFAA +M+VPT SQ +R+ Q Q GEASAARVGNYLP+TQGHLNLLASLSG Q S GRR++D
Sbjct: 18 QQQAPTGVGTNGALTVKKPPVKDRHSKVDGRGRRIRMPIVCAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTIPASFSTVSVSVRNSTASLVSSLSAPLDQKSS-----QMISPAPFILGKRLRSDDENIENGNKDDVAVAAGGATAVGPTAGFWAVPARPDFGQIWSFAAAPPPEMMVPTSAAAAAAAAAALSSQSSRFFQQQMGEASAARVGNYLPMTQGHLNLLASLSGPPQPS-SGRRDDD 264
Match: A0A3Q7FZY8 (TCP domain-containing protein {ECO:0000259|PROSITE:PS51369})
HSP 1 Score: 287.73 bits (735), Expect = 9.441e-96
Identity = 160/253 (63.24%), Postives = 183/253 (72.33%), Query Frame = 0
Query: 13 QKQLATATPTNGTVAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPANFSTVSVSLRNSN----NSLSAPLEQKQAPQLNQHLFTPTPFILGKRAREEDSIRAGEEKSD----EVSEHSVGPAGGFWALPGRPDYGQVWSFAAG--SDMVVPTPVNM---------SQQNRYMQVQ-GEASAARVGNYLPITQGHLNLLASLSGSHQQSGGGRREED 245
Q+Q T TNG + VKKPP KDRHSKVDGRGRRIRMPI+CAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGT PA+FSTVSVS+RNS +SLSAPL+QK + + +P PFILGKR R +D K D + +VGP GFWA+P RPD+GQ+WSFAA +M+VPT SQ +R+ Q Q GEASAARVGNYLP+TQGHLNLLASLSG Q S GRR++D
Sbjct: 17 QQQAPTGVGTNGALTVKKPPVKDRHSKVDGRGRRIRMPIVCAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTIPASFSTVSVSVRNSTASLVSSLSAPLDQKSS-----QMISPAPFILGKRLRSDDENIENGNKDDVAVAAGAATAVGPTAGFWAVPARPDFGQIWSFAAAPPPEMMVPTSAAAAAAAAAALSSQSSRFFQQQMGEASAARVGNYLPMTQGHLNLLASLSGPPQPS-SGRRDDD 263
| Match Name | Stats | Description |
|---|---|---|
| A0A2J6K9I8 | E-Value: 6.654e-108, PID: 70.49 | TCP domain-containing protein {ECO:0000259|PROSITE... [more] |
| A0A6S7ML55 | E-Value: 6.654e-108, PID: 70.49 | TCP domain-containing protein {ECO:0000259|PROSITE... [more] |
| A0A103XS39 | E-Value: 3.994e-106, PID: 71.19 | (Transcription factor, TCP {ECO:0000313|EMBL:KVH95... [more] |
| A0A2R6PGI9 | E-Value: 2.000e-100, PID: 70.39 | (Transcription factor like {ECO:0000313|EMBL:PSR90... [more] |
| A0A251SKI8 | E-Value: 1.092e-99, PID: 67.62 | (Putative TCP family transcription factor {ECO:000... [more] |
| A0A2J6K5H6 | E-Value: 8.386e-98, PID: 70.13 | TCP domain-containing protein {ECO:0000259|PROSITE... [more] |
| A0A251RML5 | E-Value: 1.687e-96, PID: 68.86 | (Putative TCP family transcription factor {ECO:000... [more] |
| A0A0V0HYT0 | E-Value: 2.026e-96, PID: 65.16 | (Putative transcription factor TCP7-like {ECO:0000... [more] |
| G3BGV4 | E-Value: 5.592e-96, PID: 63.24 | (TCP transcription factor 14 {ECO:0000313|EMBL:ADM... [more] |
| A0A3Q7FZY8 | E-Value: 9.441e-96, PID: 63.24 | TCP domain-containing protein {ECO:0000259|PROSITE... [more] |
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back to top| Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |