MGDPLAQKVFVFGSFSEEEARNLLNPSAENPVDKNGLLAGSLNNVPGLCF
GSFDADLSISLPSYSNGSANFKPEGDEGKATQSVNVKVNGDVENSLHCSI
IGNGSKGTTKEDELSESVLDDQVGSLEKLRLTSQDHNVVSLKHGSLNGTS
HGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLV
KLLLELKSHSISKVNYPTLAAFVDFVSVLEPNGITLKKKDIHVLETGRPF
SPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK
SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLR
SVVKARAT*
| Relationships |
|---|
| The polypeptide, DCAR_031617, derives from mRNA, DCAR_031617. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A5B7C7I4 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 345.895 bits (886), Expect = 7.435e-113
Identity = 207/380 (54.47%), Postives = 258/380 (67.89%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLNPSAEN---PVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKP----EGDEGKATQSVN---------VKVNGDVENSLHCSIIGNGSKGTTKEDELSESVL---DDQVGS----LEKLRLTSQDHNVVSLKHGSLNGT-----SHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK-SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKARA 357
KV VFGSFSE+E R+ L+ S+EN PV+K L GS N V +CFG F+ + S L S S G ++P E ++G++T++ N +K NG ++NS H S + NG+K D + + L ++ GS L+L + D+ S G+LNGT + ++++ K +G +DL PRGLIN GNLCFLNATLQALLSC P V+LL L+ +I KV YPTL+AF +FVS + P+G LKKKD VLETGRPF P+MF+ VL+ FTPDV +SISGRPRQEDAQEFLSF+MDQ+HDELLKLEG+ SS NG K SLVSS+DDDEWETVGPKNKSAVTRTQNFVPS LS IFGGQLRSVVKAR
Sbjct: 5 KVLVFGSFSEDETRSWLSQSSENAIKPVEKKDLQVGSSNFVTEICFGDFNGEQSRQLDS-SKGPTGYQPSSIQEDNKGRSTKTENDLLPGALGSLKENGGIQNSAHYSPLSNGNKEFKMTDGIKLTTLCVSEEDKGSPLNQFSTLKLHALDNG--SSGEGNLNGTVDNLSAVAHVEEETRKESNGSVSAFKDLLPRGLINPGNLCFLNATLQALLSCSPFVQLLEGLRIRNIPKVGYPTLSAFAEFVSDFDMPSGEFLKKKD--VLETGRPFKPAMFDGVLRNFTPDVPNSISGRPRQEDAQEFLSFIMDQLHDELLKLEGQLSSLNGVKSSLVSSADDDEWETVGPKNKSAVTRTQNFVPSELSAIFGGQLRSVVKARG 379
Match: A0A5N5HLG7 ((Ubiquitin carboxyl-terminal hydrolase 24-like {ECO:0000313|EMBL:KAB2627463.1}))
HSP 1 Score: 332.028 bits (850), Expect = 3.508e-107
Identity = 202/380 (53.16%), Postives = 249/380 (65.53%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLL---NP----SAENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKP-----EGDEGKATQSV--NVKV-------NGDVENSLHCSIIGNGSKGTTKED--------ELSESVLDDQVGSLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKS-SGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
K+ VFGSFSE+E R+LL +P AE PV+KN L GS+N V F+ + S S +P + DE KA ++V N+ V NG +N ++CS NG K T ++ E VL +Q SLE L +++ N GS++ S ++ +KA + P + L PRGLINSGNLCFLNATLQALLSC P V+LL EL++ + KV YPTL+AF +FVS + P+G + K KD VLETGRPFSP+MFE VLK FTPDV +SISGRPRQEDAQEFLSF+MDQMHDELLKLEG+S S NG K SL+SS++DDEWETVGPKN SAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 5 KLLVFGSFSEDETRSLLLKQSPVKGLKAEKPVEKNVLQFGSINFVTAKPSVQFNGE-SSGQKSSLKAPTKLQPLISLNKQDEVKAVKAVDGNLPVSSATPAENGCTDNYVNCSAHSNGVKEVTTDNVNLTSLPLSKDEGVLSNQFSSLE---LQNREQN------GSVDDLSVSKIKGELQKASNAPVTVSKSLLPRGLINSGNLCFLNATLQALLSCSPFVQLLQELRTPEVPKVGYPTLSAFAEFVSEFDMPSGSSSKDKDTSVLETGRPFSPAMFEGVLKNFTPDVPTSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQSPSINGRKSSLISSAEDDEWETVGPKNTSAVTRTQSFVPSELSDIFGGQLRSVVKAR 374
Match: A0A498KIB0 (USP domain-containing protein {ECO:0000259|PROSITE:PS50235})
HSP 1 Score: 327.02 bits (837), Expect = 5.822e-107
Identity = 197/377 (52.25%), Postives = 247/377 (65.52%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLL---NP-SAENPVDKNGLLAGSLNNVPGLCFGSFDADLS-----ISLPSYSNGSANFKPEGDEGKATQSVNVKV---------NGDVENSLHCSIIGNGSKGTTKED--------ELSESVLDDQVGSLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKS-SGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
K+ VFGSFSE+E R+LL +P AE PV+KN L GS+N V F+ + S + P+ ++ K DE KA ++V+ + NG +N ++CS NG K T ++ E L +Q SLE L +++ N GS++ S ++ +K + P + L PRGLINSGNLCFLNATLQALLSC P V+LL EL++ + KV YPTL+AF +FVS + P+G + K +D+ VLETGRPFSP+MFE VLK FTPDV SISGRPRQEDAQEFLSF+MDQMHDELLKLEG+S S NG K SL+SS +DDEWETVGPKN SAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 5 KLLVFGSFSEDETRSLLVKQSPVKAEKPVEKNVLQFGSINFVSAKSSVQFNGESSGQKSSLKAPTKLQPLSSLK--QDEVKAVKAVDGNLPASSATPAENGCTDNYVNCSAHSNGVKEVTTDNVNLTSLPLSKEEGGLSNQFSSLE---LQNREQN------GSVDDLSVSKIKGELQKTSNAPVTVSKSLLPRGLINSGNLCFLNATLQALLSCSPFVQLLQELRTLEVPKVGYPTLSAFAEFVSEFDMPSGSSSKDRDVSVLETGRPFSPAMFEGVLKNFTPDVLPSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQSPSINGRKSSLISSPEDDEWETVGPKNTSAVTRTQSFVPSELSDIFGGQLRSVVKAR 370
Match: D7SQ20 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 328.561 bits (841), Expect = 4.439e-106
Identity = 208/379 (54.88%), Postives = 245/379 (64.64%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLN-PS--AENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNG-----SANFKPEGDEGKATQSVNV---------KVNGDVENSLHCSIIGNGSKGTTKEDELSESVLDDQVGSLEKLRLT---SQDHNVVS---LKHGSLNGTSHGN-----LQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKSSG-NGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
K+ +FGSF+E+E R+LL PS AE P K L GS++ V G S +L S S G S+N + DE K+ VN K NG V + H S +GNG D + LD S K L S + +VV K LNGT + L+K KA +GPA V++L PRGLINSGNLCFLNATLQALLSC P ++LL EL+ I KV YPTL AF +FVS + P ++LKKKD++ +ETGRPFSP+MFE VLK FTPDV + ISGRPRQEDAQEFLSFVMDQMHDELLKLEG S NGGK SLVSS++DDEWETVGPKNKSAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 8 KLLLFGSFTEDETRSLLRLPSGNAEKPAAKKELQFGSVDFVTGRALASVGNELCRQADS-SKGPIDFCSSNLLKKDDETKSVDGVNKLSSEAAGTPKENGGVHDFTHSSPLGNGVVNELNPDRFN---LDSLCLSQNKEHLNQFQSLESHVVEEECSKERDLNGTIDDSSTFVPLKKEILKAPNGPAPAVKNLLPRGLINSGNLCFLNATLQALLSCTPFIQLLQELRIRDIPKVGYPTLTAFAEFVSEFDMPTDLSLKKKDLNGVETGRPFSPAMFEGVLKNFTPDVPNKISGRPRQEDAQEFLSFVMDQMHDELLKLEGFFSNLNGGKSSLVSSAEDDEWETVGPKNKSAVTRTQSFVPSELSKIFGGQLRSVVKAR 382
Match: A0A2R6RA35 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 323.553 bits (828), Expect = 3.444e-104
Identity = 197/373 (52.82%), Postives = 245/373 (65.68%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLNPSAEN---PVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKP----EGDEGKATQSVNV---------KVNGDVENSLHCSIIGNGSKGTTKEDEL---SESVLDDQVGSLEKLRLTS-QDHNVVSLKHGSLNGTSHG--NLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK-SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
KV +FGSF+E+E R+ LN S+E PV+K L + N V L FGSF+ + L + S G+ +P + +G ++ N K NG V+ + H + NGS+ ED + S V D ++G + + QD + K +LNGT ++ +KA D P V DL PRGLINSGNLCF+NATLQALLSC P V LL L+S +I KV YPTLAAF +F++ + P +LKKKD+ +LETGRPF P+MFEVVLK FTPDV +SISGRPRQEDAQEFLSFVM QMHDELLK EG+ S+ NG K SLVS ++DDEWETVGPKNKSAVTRTQ+FVPS LS IFGGQLRSVVKA+
Sbjct: 5 KVLLFGSFTEDETRSFLNQSSEKTEKPVEKKELPS---NVVKELSFGSFNGEPHRQL-NISRGAIASQPSDIHKDTKGSGVRTTNAVLPITSESSKENGVVQKTAHYHPLSNGSEELRIEDSINITSGHVSDRKLGPPNQPSYSKVQDLDSGISKEVNLNGTISPIVPFEEVAKKASDAPVIAVRDLLPRGLINSGNLCFINATLQALLSCSPFVHLLHGLRSSNIPKVGYPTLAAFAEFLAEFDCPGDPSLKKKDMAILETGRPFRPTMFEVVLKNFTPDVPNSISGRPRQEDAQEFLSFVMHQMHDELLKFEGQLSNSNGEKSSLVSFAEDDEWETVGPKNKSAVTRTQSFVPSDLSAIFGGQLRSVVKAK 373
Match: A0A251QA48 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 322.783 bits (826), Expect = 5.752e-104
Identity = 196/375 (52.27%), Postives = 246/375 (65.60%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLNPS----AENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYS-NGSANFKP-----EGDEGKATQSVNVKV---------NGDVENSLHCSIIGNGSKGTTKED-ELSESVLDDQVGSLE----KLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK-SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
K+FVFGSFSE+E R+LL AE PV+KN L GS+N V + G + + S P S G + +P + +E +A ++ N + NG +N + S NG K TK++ +++ L + G L L L +++ N G ++ S ++ +K +GP L PRGLINSGNLCFLNATLQALLSC P V+LL EL++ + KV YPTL AF +FVS + P+G + K KD VLETGRPFSP+MFE VLK FTPDV +SISGRPRQEDAQEFLSF+MDQMHDELLKLEG+ SS NG K SLVSS+++DEWETVGPKN SAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 5 KLFVFGSFSEDETRSLLQKQSPGKAEKPVEKNVLQFGSINFVTKISSGECNGE--SSRPQNSVKGLSKVQPLSSLNKQNEVEAVKAANDSLPASLTTPTENGCTDNYKNGSAHSNGVKEATKDNIDVASLPLSNNEGGLSNKFSSLELQNREQN------GWVDDLSASKVKGELQKCLNGPVTVSTVLLPRGLINSGNLCFLNATLQALLSCSPFVQLLQELRTRKVPKVGYPTLGAFAEFVSEFDMPSGSSSKNKDASVLETGRPFSPAMFEGVLKIFTPDVPTSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQPSSINGRKSSLVSSAEEDEWETVGPKNTSAVTRTQSFVPSELSDIFGGQLRSVVKAR 371
Match: A0A067KWA3 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 322.783 bits (826), Expect = 6.554e-104
Identity = 197/381 (51.71%), Postives = 238/381 (62.47%), Query Frame = 0
Query: 5 LAQKVFVFGSFSEEEARNLLN-PSAEN---PVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKPEGDEGKATQSVNV-----------KVNGDVENSLH---CSIIGNGSKGTTKEDELSESVLDDQ----------VGSLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLEPNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKS-SGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
++ KV VFGSF+E+EAR+ L PS+E PV K L GSLN G+ FG+F++ S L S +NG F K T+ N K NG + N H S + NG K E S+ Q + + L + D N++ K S ++QK DGP V +L PRGLINSGNLCFLNA+LQALLSC P ++LL +L+ +I KV +PTL AF +F S + + KKD ++ETGRPFSPSMFE VLK FTPDV +SISGRPRQEDAQEFLSF+MDQMHDELLKLEG+S NG K SLVSSS+DDEWETVGPKNKSAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 1 MSNKVLVFGSFTEDEARSWLQKPSSETDEKPVAKKELQFGSLNFDTGITFGNFNSASSRQLDS-ANGKVGFHTTSSLNKDTKIANKADNLPGLLRSPKENGSINNFSHRISLSNLTNGVKDLKMESFDLTSLHSSQNEDSSLNQSQSSNFDVLDVLDSDGNMMGSKSDSTVYVKREDIQKPT----DGPVAAVTNLLPRGLINSGNLCFLNASLQALLSCSPFIQLLQDLRIRNIPKVGFPTLTAFAEFFSKFDAPSSSKLKKDTTIIETGRPFSPSMFEGVLKNFTPDVPNSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQSICQNGAKSSLVSSSEDDEWETVGPKNKSAVTRTQSFVPSELSDIFGGQLRSVVKAR 376
Match: A0A314UV85 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 321.627 bits (823), Expect = 1.583e-103
Identity = 194/372 (52.15%), Postives = 241/372 (64.78%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLNPS-----AENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYS-NGSANFKPEGDEGKATQSVNVKV--------------NGDVENSLHCSIIGNGSK-GTTKEDELSESVLDDQVGSLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK-SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
K+FVFGSFSE+E R+LL AE P++KN L GS+N V + G + + S P S G + +P K ++ +VK NG +N + S NG K TT +++ L + G L T+Q ++ ++G ++ S ++ +K DGP L PRGLINSGNLCFLNATLQALLSC P V+LL EL++ + KV YPTL AF +FVS + P+G + K KD VLETGRPFSP+MFE VLK FTPDV +SISGRPRQEDAQEFLSF+MDQMHDELLKLEG+ SS N K SLVSS+++DEWETVGPKN SAVTRTQ+FVPS LS+IFGGQLRSVVKAR
Sbjct: 5 KLFVFGSFSEDETRSLLQKQSPPGKAEKPMEKNVLQFGSINFVSKISSGECNGE--SSRPQNSVKGLSKVQPLSSLNKQNEAESVKAPNDSLPASLTTPTENGCTDNYKNGSAHSNGVKEATTDNIDVASLPLSNNEGGL-----TNQFSSLNREQNGRVDDLSASKVKGELQKCLDGPLTVSTVLLPRGLINSGNLCFLNATLQALLSCSPFVQLLQELRTRKVPKVGYPTLGAFAEFVSEFDMPSGSSSKTKDASVLETGRPFSPAMFEGVLKNFTPDVPTSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQPSSINRRKSSLVSSAEEDEWETVGPKNTSAVTRTQSFVPSELSDIFGGQLRSVVKAR 369
Match: A0A6I9TZJ9 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 320.857 bits (821), Expect = 2.812e-103
Identity = 195/371 (52.56%), Postives = 238/371 (64.15%), Query Frame = 0
Query: 1 MGDPLAQKVFVFGSFSEEEARNLLNPS---AENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKPEGDEG--------KATQSV-NVKVNGDVENSLHCSIIGNGSKGTTKEDELSESVLDDQVG--SLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKSSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
MGDP KV +FGSFSE+E R+ LN + A+ P + L S N L FGSF + +S +L + NG N P + AT+ + +V NG + NS H + L S +D + S+ +L DH ++L G N T + K D P ++ PRGLINSGNLCFLNATLQALLSC P V+LL LK+ +I KV YPTLAAF +F+ + +G ++KKKD+ VLE GRPFSP+MFE VL+KFTPDVSSSISGRPRQEDAQEFLSF+MDQMHDELLKLEG+S +G SLVS+S+DDEWETVGPKNKSAVTRTQ F+PS LS IFGGQLRSVVKAR
Sbjct: 1 MGDP--SKVLLFGSFSEDETRSWLNQTPVNAQKPGGRKELQVNSSNLGQVLTFGSFSS-ISDTLVAVPNGPGNMHPSAIQKDNKDLCAKSATELLPSVPENGKLHNSNHSHHGDHEITDGINFRALHVSDIDSETDGKSISEL----PDHESINLNGGINNPTVEAH-HKEARLKTDVPVLDTREILPRGLINSGNLCFLNATLQALLSCSPFVRLLQGLKNRNIPKVGYPTLAAFAEFIGEFDMVSGTSVKKKDVTVLEIGRPFSPTMFEGVLRKFTPDVSSSISGRPRQEDAQEFLSFIMDQMHDELLKLEGQSHFDGRNSSLVSTSEDDEWETVGPKNKSAVTRTQTFLPSELSSIFGGQLRSVVKAR 363
Match: A0A7J0F0M4 (Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025} (3.4.19.12 {ECO:0000256|RuleBase:RU366025}))
HSP 1 Score: 320.857 bits (821), Expect = 4.240e-103
Identity = 196/374 (52.41%), Postives = 249/374 (66.58%), Query Frame = 0
Query: 8 KVFVFGSFSEEEARNLLNPSAEN---PVDKNGLLAGSLNNVPGLCFGSFDAD----LSISLPSYSNGSANFKPE--GDEGKATQSV------NVKVNGDVENSLHCSIIGNGSKGTTKEDELSESVL---DDQVGSLEK---LRLTSQDHNVVSLKHGSLNGTSHG--NLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLE-PNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGK-SSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKAR 356
+V +FGSF+E+E R+ LN S+E PV+K L + N V L FGSF+ + L+IS + ++ ++ + G K T +V + K NG V+ + H + NGS+ ED ++ + L D +VG + ++T D + K +LNGT ++ +KA D P V DL PRGLINSGNLCF+NATLQALLSC P V LL L+S +I KV YPTLAAF +F++ + P +LKK+D+ +LETGRPF P+MFE+VLK FTPDV SISGRPRQEDAQEFLSFVM QMHDELLK EGK S+ NG K SLVS ++DDEWETVGPKNKSAVTRTQ+FVPS LS IFGGQLRSVVKA+
Sbjct: 11 EVLLFGSFTEDETRSFLNQSSEKTEKPVEKKELPS---NVVKELSFGSFNGEPHRQLNISRGAIASQPSDIHKDTTGSGVKTTNAVLPITSESSKENGVVQKTAHYHPLSNGSEELRIEDSINVTSLHVSDRKVGPPNQPSYSKVTDLDSGIS--KEVNLNGTISPIVPFEEVAKKASDAPVIAVRDLLPRGLINSGNLCFINATLQALLSCSPFVHLLHGLRSSNIPKVGYPTLAAFAEFLAEFDCPGDPSLKKRDMAILETGRPFRPTMFELVLKNFTPDVPDSISGRPRQEDAQEFLSFVMHQMHDELLKFEGKLSNSNGEKSSLVSFAEDDEWETVGPKNKSAVTRTQSFVPSDLSAIFGGQLRSVVKAK 379
| Match Name | Stats | Description |
|---|---|---|
| A0A5B7C7I4 | E-Value: 7.435e-113, PID: 54.47 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A5N5HLG7 | E-Value: 3.508e-107, PID: 53.16 | (Ubiquitin carboxyl-terminal hydrolase 24-like {EC... [more] |
| A0A498KIB0 | E-Value: 5.822e-107, PID: 52.25 | USP domain-containing protein {ECO:0000259|PROSITE... [more] |
| D7SQ20 | E-Value: 4.439e-106, PID: 54.88 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A2R6RA35 | E-Value: 3.444e-104, PID: 52.82 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A251QA48 | E-Value: 5.752e-104, PID: 52.27 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A067KWA3 | E-Value: 6.554e-104, PID: 51.71 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A314UV85 | E-Value: 1.583e-103, PID: 52.15 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A6I9TZJ9 | E-Value: 2.812e-103, PID: 52.56 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
| A0A7J0F0M4 | E-Value: 4.240e-103, PID: 52.41 | Ubiquitin carboxyl-terminal hydrolase {ECO:0000256... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |