DCAR_031045 | CarrotOmics

Resource Type:

Polypeptide

Name:

DCAR_031045

Identifier:

DCAR_031045.mRNA-protein

Sequence:

MPILGTSASTKENLPIKKEEATVSPKAEPVLPSPDIHNDENAPRNISPID
DSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAV
ATLQSVRGIIKIQALVRGQRVRRSIGSEIREREPHKEIDDQCLSSFGNIG
SQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQV
LGPHSQFKETIGVTGSSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPE
SERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVA
GSERRKHSIGKSSKSDISEGISNPVEKVIEVSEKAESKPSDVEISQELPA
EGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVD
EEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATES
AKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGSSPRMHKLIQAS
IKGGMRGDRSLMSSRDSSDSRVIQAEWKR*

Sequence Length:

530

Sequence Checksum:

58ab43d8ee198f64bd76ade3f6c119d4

View location in JBrowse:

DCARv2_Chr9:32258293..32262562-

Organism:

Daucus carota subspecies sativus

Relationship:

There is 1 relationship.
Relationships
The polypeptide, DCAR_031045, derives from mRNA, DCAR_031045.

Blast Results:

The following BLAST results are available for this feature:

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6L5BC34 (DUF4005 domain-containing protein {ECO:0000259|Pfam:PF13178})

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 412/562 (73.31%), Postives = 442/562 (78.65%), Query Frame = 0

Query:    1 MPILGTSASTKENLPIKKEEATVSPKAEPVLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRSIGSEIREREPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTGSSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKSDISEGISNPVEKVIEVSEKAESK--------------------------------PSDVEISQELPA-EGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGSSPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            +PILGTSAS KE+ PIKK EAT+SP+AEP+L S DI+N ENAP NIS I++SEK  LEQAAI AQAAIRGYQAR+KL SL+ IT+LQALARGHLVRRQAVATLQSVRGIIKIQAL RGQ+VR+SIG EIR REP  E DDQCLSSFGNIGSQRTEMLLKTAFVGKL+S+ PAAMPLQL YEPEEPNSL NWLNRWTLLQVLGPH QFKETIGV G                   +TSS+N+NGLK +  ESE+ KVKLKKV KDPVKS QE PQNGTKKVLRNPKKVSKPLV+TSVK+VAGSERRKHSIGKSSKSDISE ISN VEKVI+VS KAESK                                PSDVE S ELPA E TDSKL+N S  PLQPSE PVQQKEEV                  TLVDEEI+ +     DENKK+SMRRASLPAKHDY EKD+HSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKF SSPR+HKL+QASIKGGMRGDRSLMSSRDSSDSR IQAEWKR
Sbjct:   33 VPILGTSASKKESPPIKKGEATISPRAEPILSSLDINNSENAPINISSINESEKMSLEQAAIMAQAAIRGYQARKKLQSLQGITKLQALARGHLVRRQAVATLQSVRGIIKIQALFRGQKVRQSIGKEIRVREPRGESDDQCLSSFGNIGSQRTEMLLKTAFVGKLLSTFPAAMPLQLRYEPEEPNSLWNWLNRWTLLQVLGPHLQFKETIGVPG-------------------LTSSENENGLKCNGLESEKFKVKLKKVAKDPVKSTQEQPQNGTKKVLRNPKKVSKPLVDTSVKSVAGSERRKHSIGKSSKSDISEAISNSVEKVIKVSGKAESKHFTGKSSKPDISEVISNPVEEVIKVSGKTEAIPSDVETSLELPAEEATDSKLNNGSNVPLQPSEAPVQQKEEV------------------TLVDEEIRAR-----DENKKSSMRRASLPAKHDYSEKDLHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFSSSPRVHKLVQASIKGGMRGDRSLMSSRDSSDSRAIQAEWKR 552

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YRG2 (DUF4005 domain-containing protein {ECO:0000259|Pfam:PF13178})

HSP 1 Score: 365.925 bits (938), Expect = 6.835e-118
Identity = 241/538 (44.80%), Postives = 315/538 (58.55%), Query Frame = 0

Query:    2 PILGTSASTKENLPIKKEEATVSPKAEPVLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIREREPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFK--ETIGVTGSSSL-SVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSK---SDISE-GISNPVEKVIEVSEKAESKPSDVEIS-QELPAEGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQA-SPNFDQDDAEKYALRRRHSLPSSNSGKFGSSPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            P+    A   EN   +K  A+  P  E +L S     D     N+    DSE+ RLEQAA + QAA RGY A     +L+ I RLQAL RGHLVRRQAVATL  V  I+K+QALVRGQ+VRRS IGS++ +++   ++D +CL S     S + E LL  AFV KL+SS PA +PL L Y+P EPNS  +WL RWT+  V G +SQ K  +    T   S  +V  ++ R + SV R+  ++ +NGL +S  ESE+ K   +KV   P  S+QEHPQN  +KV  N KK S P  ETS++    +E+ K    K+S     D+ E GI +  EK  +  + A  K SDVE S + L A+    +L  HS   LQP +  +  + E  P+                     +  K+  I++E +K S RRASLPAK DY E  +H+TPRVPSYMA T+SAKAK R Q  SP F QDD EK  L RRHSLPSS +GK   SPR+ +L+QAS KGG+R DRSL+S   S+D +VIQAEW+R
Sbjct:   59 PVPCPGAGIGEN--SEKGAASRLPNDEVILYSAQEGGDAQETTNLGSPKDSERIRLEQAATSVQAAFRGYLAHRAFQALKGIIRLQALIRGHLVRRQAVATLYCVNRIVKLQALVRGQKVRRSDIGSKLHKKQSLGKLDPKCLDSCKTNTSTQVEKLLVNAFVRKLLSSSPATVPLHLQYDPGEPNSAWDWLRRWTMSHVWGQYSQPKIIDPKSQTRHGSFQTVEAEQVRPKRSVQRLPPANVENGLNKSALESEKLKRNPRKVPNHPANSVQEHPQNEIEKVKCNLKKTSNPTTETSIRPENDTEKPKRIHRKASNIAAPDVPEQGIDDCAEKAKKDIDLAVPKASDVETSPKSLAADRPVDELHEHSAIDLQPKQ--INGRNEAIPL---------------------LSSKHDEISNEYQKTSQRRASLPAKQDYQENGLHNTPRVPSYMATTKSAKAKFRGQQHSPRFGQDDDEKNGLTRRHSLPSSANGKLILSPRVQRLVQASGKGGIRKDRSLLS---STDEKVIQAEWRR 568

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: D7TND0 (DUF4005 domain-containing protein {ECO:0000259|Pfam:PF13178})

HSP 1 Score: 347.436 bits (890), Expect = 1.294e-110
Identity = 231/527 (43.83%), Postives = 312/527 (59.20%), Query Frame = 0

Query:   19 EEATVS--PKAEPVLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIREREPHKEI-DDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTG----SSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSK--SDISE-GISNPVEKVIEVSEKAESKPSDVEISQELPAEGTDSKLDN---HSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNS-GKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            E+ T S  P    +L S   + D     N+    D E+ R EQAA  AQAA RGY AR    +L+ I RLQAL RG LVRRQA+ATL  V+GI+K QALVRGQ VR S IG+E+ E+   ++  D +C +SFG   S + E L K  FV  L++S P +MPL L Y P EPNS  +WL RWT      P ++ K+ I         +S +V TD++R + SV + TS+  +NG  +S  ES++ K  L+KV+  PV S+QEHP+N T+K     +K  K   + S +    +E+ K S+ KSS   SD  E G  + ++K+ +      SK SD+E S + PAE  +  +DN   H+ A LQ  E              K E  P   K+        +  K+  I+++++K S RRASLP KHDY E  +H+TPR+PSYMAATESAKAKLRA +SP F QD+A+K  + RRHSLPSS++ GK  S SPR  +L+QAS KG  R DRSLMSSRD S+ +++Q EW+R
Sbjct:   74 EKGTASRLPNDGVILSSSKENGDTETIMNLGLSKDPERIRHEQAATKAQAAFRGYLARRAFRTLKGIIRLQALGRGRLVRRQAIATLCCVQGIVKFQALVRGQSVRHSNIGTEVHEKLSARKFPDAKCSNSFGLQTSNQAEKLSKNVFVCTLLASSPTSMPLHLQYGPGEPNSAWDWLERWTKSHFWEPLTKPKKIIDSKSQKKRGTSQTVETDRSRPKRSVRKATSAKFENGSTQSTLESDKPKRNLRKVSSHPVDSVQEHPKNATEKTKSKLRKNLKSTSDASDQLEVKAEKPKQSLRKSSSAASDAPEQGTGDSLKKIKKDMAVTVSKQSDIETSLKPPAE--NELVDNVHDHTLADLQCVEN-----------NGKSENIPEANKD--------MSYKDNDISNDDQKTSQRRASLPGKHDYQENGLHNTPRLPSYMAATESAKAKLRALSSPRFGQDEADKNGITRRHSLPSSSTNGKLSSWSPRAQRLVQASGKGVFRSDRSLMSSRDGSE-KLLQPEWRR 578

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A834ZMY9 ((Uncharacterized protein {ECO:0000313|EMBL:KAF8408068.1}))

HSP 1 Score: 336.65 bits (862), Expect = 1.678e-105
Identity = 225/498 (45.18%), Postives = 291/498 (58.43%), Query Frame = 0

Query:   50 DDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIRER----EPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTG----SSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKS---DISE-GISNPVEKV--IEVSEKAESKPSDVE-ISQELPAEG-TDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            +D E+ R EQAA  AQAA RGY AR    +L+ I RLQAL RGHLVRRQAVATL  ++GI+K+QALVRG+RVR S IG E+R+      P       C    G   S RTE L    F  KL+SS P  MPL+L Y  EEPNS   WL  WTL +   P SQ K+ +         +  +VGT+  R + SV  + +   D+G   S P+SE+ K  L+KV   PV S+QEHPQN  +KV RN +KVS   +E S +A   +E+ K S+  SS+    D SE G S+  EK   + V+   +SK SDV+ I + L A+   D   D+HS A LQP E+                     + E + +++ E+  K    ++ N++ S RRAS PAK +     I STP +PSYMA T+SAKAKLR Q SP F QD+AEK    RRHSLPSS +GK  S  PR HKL+QAS KGG++ DRSL+SSRD  + +VIQA W+R
Sbjct:  182 NDPERIRQEQAATKAQAAFRGYLARRAFRALKGIIRLQALVRGHLVRRQAVATLCCMQGIVKLQALVRGRRVRLSGIGLEVRQNCNLGNPLVAKRSSCS---GVNTSPRTEKLPANVFASKLLSSSPNVMPLRLWYCLEEPNSAGKWLECWTLSRFWKPISQPKKILDTKSKRKRGNFQAVGTEPGRPKHSVRSVPAPTVDSGSLHSTPKSEKPKRNLRKVLSHPVDSVQEHPQNELEKVKRNLRKVSNSTMEASDRADVETEKPKCSLRNSSRFPAPDASEQGTSDSAEKKKDLTVAVSKDSKQSDVDTIPKPLAADDPVDVLQDDHSTAELQPLESG-------------------GSDENIPMINGELSSKEDQTSNGNQETSKRRASFPAKQECTGNGIQSTPTLPSYMAPTKSAKAKLRGQGSPMFGQDEAEKNGFTRRHSLPSSTNGKLSSLLPRTHKLVQASGKGGIKSDRSLLSSRDGKE-KVIQAGWRR 656

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A5BAX7 (DUF4005 domain-containing protein {ECO:0000259|Pfam:PF13178})

HSP 1 Score: 333.954 bits (855), Expect = 3.119e-105
Identity = 225/524 (42.94%), Postives = 301/524 (57.44%), Query Frame = 0

Query:   19 EEATVS--PKAEPVLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGY----------QAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIRER-EPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTG----SSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSK--SDISE-GISNPVEKVIEVSEKAESKPSDVEISQELPAEG-TDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNS-GKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSS 518
            E+ T S  P    +L S   + D     N+    D E+ R EQAA  AQAA RGY          +AR    +L+ I RLQAL RG LVRRQA+ATL  V+GI+K QALVRG+ VR S IG+E+ E+    K +D +C +SFG   S + E L K  FV  L++S P +MPL L Y P EPNS  +WL RWT      P ++ K+ I         +S +V TD++R + SV + TS+  +NG  +S  ES++ K  L+KV+  PV S+QEHP+N T+K     +K  K   + S +    +E+ KHS+ KSS   SD  E G  + ++K+ +      SK SD+E S + PAE      + +H+ A LQ  E     K E  P   K+                 +  K+  I+++++K   RRASLP KHDY E  +H+TPR+PSYMAATESAKAKLRA  SP F QD+A+K  + RRHSLPSS++ GK  S SPR  +L+QAS KG  R DRSLMSSRD S
Sbjct:   87 EKGTASRLPNDGVILSSSKENGDTETIMNLGLSKDPERIRHEQAATKAQAAFRGYLFTDASLISLKARRAFRTLKGIIRLQALGRGRLVRRQAIATLCCVQGIVKFQALVRGRSVRHSNIGTEVHEKLSARKFLDAKCSNSFGLQTSNQAEKLSKNVFVCTLLASSPTSMPLHLQYGPGEPNSAWDWLERWTKSHFWEPLTKPKKIIDSKSQKKRGTSQTVETDRSRPKRSVRKATSAKFENGSTQSTLESDKPKCNLRKVSSHPVDSVQEHPKNATEKTKSKLRKNLKSTSDASDQLEVKAEKPKHSLRKSSSAASDAPEQGTGDSLKKIKKDMAVTVSKQSDIETSLKPPAENELVDDVHDHTLADLQCVEN--NGKSENIPEANKD-----------------MSYKDNDISNDDQKTXQRRASLPGKHDYQENGLHNTPRLPSYMAATESAKAKLRALGSPRFGQDEADKNGITRRHSLPSSSTNGKLSSWSPRAQRLVQASGKGVFRSDRSLMSSRDGS 591

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5AT29 (DUF4005 domain-containing protein {ECO:0000259|Pfam:PF13178})

HSP 1 Score: 331.257 bits (848), Expect = 1.546e-104
Identity = 220/535 (41.12%), Postives = 298/535 (55.70%), Query Frame = 0

Query:    2 PILGTSASTKENLPIKKEEATVS--PKAEPVLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIREREPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGV---TGSSSL-SVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKSDISEGISNPVEKVIEVSEKAESKPSDVEISQELPAEGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGSSPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            P+  + A   EN     E    S  P  E VL S     D     N+    DS++  LE+AA + QAA RGY AR     L+ I RLQAL RGHLVRRQAVATL  V  I+K+QALVRGQ+VRRS IGS++ +++   ++D +CL SF    S + E LL  AFV KL+SS    +PL L Y P EPNS   WL RWT+  V G HS   + I +   T   S  +V  ++ R + SV R+ +++ D+   +S  ESE+ K   +KV+     S++EHPQN T+K + +                       H+  + +K DI  G++ P    +E S K+        ++ ++PA+    +L  HS   LQP +   +                    E++ LVD+ +  K+  I++E +K S RRASLPAK DY E  +H TPRVPSYMA TESAKAK R Q SP F QD+AEK  L RRHSLP+S +GK   SPR+ +L+QAS KGG+R DRSL+S   S+D + IQAEW+R
Sbjct:   91 PVYCSGAGIGEN----SENGVASRLPNDEVVLYSAKQDGDAQKTTNLGSPKDSKRIGLEEAATSVQAAFRGYLARRAFQVLKGIIRLQALIRGHLVRRQAVATLYCVNRIVKLQALVRGQKVRRSDIGSKVHKKQGLGKLDPKCLDSFETNTSNQVEKLLVNAFVCKLLSSSHTTVPLYLQYGPGEPNSAWEWLRRWTMSHVWG-HSSQPKIIDLKPQTKDGSFQTVEAEQGRPKRSVQRLPTANVDSVSDKSALESEKLKRNQRKVSNHTANSVKEHPQNETEKGVDD-----------------------HA--EKAKRDI--GLAVPGRSYVETSPKS--------LAADVPAD----ELHVHSAIDLQPKQINGRN-------------------EDIPLVDKSLSSKHDDISNEYQKTSRRRASLPAKQDYQENGLHKTPRVPSYMATTESAKAKFRGQLSPRFGQDEAEKNGLTRRHSLPTSANGKLSLSPRVQRLVQASGKGGIRSDRSLLS---STDEKAIQAEWRR 559

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1U7YQM9 ((protein IQ-DOMAIN 31 isoform X1 {ECO:0000313|RefSeq:XP_010242390.1}))

HSP 1 Score: 329.717 bits (844), Expect = 8.502e-104
Identity = 215/492 (43.70%), Postives = 284/492 (57.72%), Query Frame = 0

Query:   50 DDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS--IGSEIREREPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKET----IGVTGSSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKSDISE----GISNPVEKVIEVSEKAESKPSDVEIS-QELPAEGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            +D E+ R E+AA  AQAA RGY AR    +L  I RLQAL RGHLVRRQA+ATL  +RG++K+QALVRG+R R S  +    ++    K +D + L SFG      TE   + AFV K+++S   AMPL L Y  +EPNS  NWL+RWT  +   P  Q K+     + +   +  +  T+  R + SV R  S   DNG   S  ESE+ K  L+KVT  PV ++QEHPQN  +KV RN +KVS   VE   +    +E+ K S+ K+  S   E    G+    EK+ + +   ESK  +VE S + L  +G  S L+N                    PV + E +      E + L +EE+  K    ++EN+K S RRAS PAK DY E  + +TP +PSYMA TESAKAKLRAQ SP F  D AEK    RRHSLPSS +GK  S SPR  +L+QAS KGG+R D SL+SSRD ++ ++IQAEW+R
Sbjct:  105 NDPERIRQEKAATKAQAAFRGYLARRAFRALRGIIRLQALVRGHLVRRQALATLHCMRGVVKLQALVRGRRARLSDTVLKVHKKYRLVKALDSKQLESFG-----WTEKPSENAFVCKILASSSVAMPLHLQYGLDEPNSAWNWLDRWTSFRFWRPLPQPKKVADSKLQIKQVNVQNAETEPGRQKRSVRRFGSGSVDNGPTNSNSESEKPKRSLRKVTSHPVDAVQEHPQNEVEKVKRNLRKVSNSTVEVPDRIEVENEKPKRSVRKAPSSMAPEVSDQGMRESAEKMKKDTMVPESKQLNVETSPKPLTPDGPTSVLNNDH------------------PVVELELLQSNGKDENVHLTNEELISKEDPASNENQKTSRRRASFPAKQDYPENGLQNTPSLPSYMATTESAKAKLRAQGSPRFGHDGAEKNGFTRRHSLPSSTNGKLSSVSPRTQRLVQASGKGGVRSDGSLLSSRDGNE-KIIQAEWRR 572

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1U7YTE2 ((protein IQ-DOMAIN 31 isoform X2 {ECO:0000313|RefSeq:XP_010242391.1}))

HSP 1 Score: 327.791 bits (839), Expect = 4.012e-103
Identity = 215/492 (43.70%), Postives = 282/492 (57.32%), Query Frame = 0

Query:   50 DDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS--IGSEIREREPHKEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKET----IGVTGSSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKSDISE----GISNPVEKVIEVSEKAESKPSDVEIS-QELPAEGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            +D E+ R E+AA  AQAA RGY AR    +L  I RLQAL RGHLVRRQA+ATL  +RG++K+QALVRG+R R S  +    ++    K +D + L SFG      TE   + AFV K+++S   AMPL L Y  +EPNS  NWL+RWT  +   P  Q K+     + +   +  +  T+  R + SV R  S   DNG   S  ESE+ K  L+KVT  PV ++QEHPQN  +KV RN +KVS   VE   +    +E+ K S+ K+  S   E    G+    EK+ + +   ESK  +VE S + L  +G  S L+N                    PV + E +      E + L +EE+  K    ++EN+K S RRAS PAK DY E  + +TP +PSYMA TESAKAKLRAQ SP F  D AEK    RRHSLPSS +GK  S SPR  +L+QAS KGG+R D SL+SSR   D ++IQAEW+R
Sbjct:  105 NDPERIRQEKAATKAQAAFRGYLARRAFRALRGIIRLQALVRGHLVRRQALATLHCMRGVVKLQALVRGRRARLSDTVLKVHKKYRLVKALDSKQLESFG-----WTEKPSENAFVCKILASSSVAMPLHLQYGLDEPNSAWNWLDRWTSFRFWRPLPQPKKVADSKLQIKQVNVQNAETEPGRQKRSVRRFGSGSVDNGPTNSNSESEKPKRSLRKVTSHPVDAVQEHPQNEVEKVKRNLRKVSNSTVEVPDRIEVENEKPKRSVRKAPSSMAPEVSDQGMRESAEKMKKDTMVPESKQLNVETSPKPLTPDGPTSVLNNDH------------------PVVELELLQSNGKDENVHLTNEELISKEDPASNENQKTSRRRASFPAKQDYPENGLQNTPSLPSYMATTESAKAKLRAQGSPRFGHDGAEKNGFTRRHSLPSSTNGKLSSVSPRTQRLVQASGKGGVRSDGSLLSSR---DEKIIQAEWRR 570

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1U8Q610 ((protein IQ-DOMAIN 31-like {ECO:0000313|RefSeq:XP_010256684.1, ECO:0000313|RefSeq:XP_019053486.1}))

HSP 1 Score: 319.701 bits (818), Expect = 6.021e-100
Identity = 221/498 (44.38%), Postives = 289/498 (58.03%), Query Frame = 0

Query:   50 DDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRSIGSEIREREPH---KEIDDQCLSSFGNIGSQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTGSSSL-------SVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKS---DISE-GISNPVEKVIEVSEKAESKPSDVEISQELPAEGTDSKLDNHSYAPLQPS-ETPVQQ--KEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            +D E+ RLE+AA  AQAA RGY AR    +L  I RLQAL RGHLVRRQA+ATL  ++GI+K+QALVRG+RVR  + + +R    +   K +D +   SFG      TE     AFV KL+SS  A MPL + Y+ EEPNS  +WL+RWT  +   P  Q K    VT S S        +V T+  + + SV R +S++ DNG   S  ESE+ K  L+KVT  PV S+QEHPQN  +KV RN +KVS  ++E S      +E+ K S+ K+  S   ++SE   S+  EK  + +  A SK  DVE S   P   T   L N+      P  E P+Q   K+E  PV   E       KE+ T             ++E +K S RRAS P K +Y E  + +TP +PSYMA TESAKAKLR Q+SP F  D A+K    RRHSLPSS +GK  S SPR  +L+Q S KGG+R D+SL SSRD ++ R++QAEW+R
Sbjct:  105 NDPERIRLEKAATKAQAAFRGYLARRAFRALRGIIRLQALVRGHLVRRQAIATLHCIQGIVKLQALVRGRRVRL-LDTSLRVHRKYSSTKALDAKWSGSFG-----WTEKPSVNAFVCKLLSSSSAVMPLCIQYDLEEPNSAWSWLHRWTSSRFWIPLPQPKV---VTDSKSQAKQGNTQTVETELGKPKRSVRRFSSANVDNGSINSTSESEKPKRNLRKVTSHPVDSVQEHPQNELEKVKRNLRKVSNSILEVSDCVEVENEKPKRSLRKAPISPAPNVSEQDTSDCAEKTNKDATVAVSKQLDVETS---PKPVTPDGLANNVLIDDHPVIELPLQSNVKDENVPVTNGESTF----KEDTT-------------SNEKQKTSRRRASFPVKQEYPENGLPNTPSLPSYMATTESAKAKLRTQSSPRFSHDGADKNGFTRRHSLPSSTNGKLISVSPRTQRLVQTSGKGGIRSDKSLFSSRDGNE-RIVQAEWRR 572

[Close]

BLAST of DCAR_031045 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A834Z5V8 ((Uncharacterized protein {ECO:0000313|EMBL:KAF8399431.1}))

HSP 1 Score: 316.62 bits (810), Expect = 4.747e-97
Identity = 218/520 (41.92%), Postives = 294/520 (56.54%), Query Frame = 0

Query:   30 VLPSPDIHNDENAPRNISPIDDSEKRRLEQAAITAQAAIRGYQAREKLHSLEAITRLQALARGHLVRRQAVATLQSVRGIIKIQALVRGQRVRRS-IGSEIREREP-HKEIDDQCLSSFG--------NIG---SQRTEMLLKTAFVGKLISSMPAAMPLQLCYEPEEPNSLSNWLNRWTLLQVLGPHSQFKETIGVTGSSSLSVGTDKARSRGSVSRITSSDNDNGLKRSVPESERNKVKLKKVTKDPVKSIQEHPQNGTKKVLRNPKKVSKPLVETSVKAVAGSERRKHSIGKSSKS----DISEGISNPVEKVIEVSEKAESKPSDVE-ISQELPA-EGTDSKLDNHSYAPLQPSETPVQQKEEVTPVQQKEEVTPVQTKEELTLVDEEIKKKNALINDENKKASMRRASLPAKHDYLEKDIHSTPRVPSYMAATESAKAKLRAQASPNFDQDDAEKYALRRRHSLPSSNSGKFGS-SPRMHKLIQASIKGGMRGDRSLMSSRDSSDSRVIQAEWKR 529
            VL S + + D      +   +D E+ R EQAA  AQAA RGY AR    +L  I RLQAL RGHLVRRQAVATL+ ++GI+K+QALVRG+ VR S  G E+ ++    + +D +   S G        ++G   S RTE LL   FV KL++S P AMPL+L Y  EEPNS   WL  WT+ +   P SQ K+ +         V  +  R + SV  + +++ DNG   S  +SE+ K  L+K +  PV S+QEHPQN  +KV RN +KVS    E S      +E+ K S+ K+S S     + +G S+P EK  + +     +P DVE I + + A E  D   D+HS   LQ  E     K++ +PV                L  +E +  N      N+K S RRAS PAK +Y   D+ +TP +PSYMA+TESAKA+LR Q SP F QD AEK    RRHSLPSS +GK  S +P+  +L+QAS K G+R DRSL+SS +    +V+QAEW+R
Sbjct:  232 VLLSVNQNADTQGTTGLRSPNDPERIRQEQAATKAQAAFRGYLARRAFRALRGIIRLQALVRGHLVRRQAVATLRCMQGIVKLQALVRGRSVRFSDTGLEVHKKGSLGRPLDAKHSDSLGVNTSTRTDSLGVNTSTRTEKLLANVFVSKLLASSPTAMPLRLRYGQEEPNSAWKWLECWTMSRFWEPLSQPKKVLDSKSQRKRGVEAEPGRPKRSVRSVPAANVDNGSLHSTSKSEKPKRNLRKSSSHPVDSVQEHPQNELEKVKRNLRKVSNSTTEASDPPEVETEKPKSSLRKTSASPAPDALEQGTSDPSEKKKDPTVVISKQP-DVETIPKPMAADEPVDVLHDDHSTVELQYLEN--SGKDDNSPVTNG------------VLCSKEDQTSNG-----NQKTSKRRASFPAKQEYTGNDVQNTPTLPSYMASTESAKARLRGQGSPRFSQDGAEKNGFTRRHSLPSSTNGKLSSPTPQTQRLVQASGKCGIRSDRSLLSSNE----KVVQAEWRR 727


Match Name	Stats	Description
A0A6L5BC34	E-Value: 0.000e+0, PID: 73.31	DUF4005 domain-containing protein {ECO:0000259\|Pfa... [more]
A0A5B6YRG2	E-Value: 6.835e-118, PID: 44.80	DUF4005 domain-containing protein {ECO:0000259\|Pfa... [more]
D7TND0	E-Value: 1.294e-110, PID: 43.83	DUF4005 domain-containing protein {ECO:0000259\|Pfa... [more]
A0A834ZMY9	E-Value: 1.678e-105, PID: 45.18	(Uncharacterized protein {ECO:0000313\|EMBL:KAF8408... [more]
A5BAX7	E-Value: 3.119e-105, PID: 42.94	DUF4005 domain-containing protein {ECO:0000259\|Pfa... [more]
A0A5J5AT29	E-Value: 1.546e-104, PID: 41.12	DUF4005 domain-containing protein {ECO:0000259\|Pfa... [more]
A0A1U7YQM9	E-Value: 8.502e-104, PID: 43.70	(protein IQ-DOMAIN 31 isoform X1 {ECO:0000313\|RefS... [more]
A0A1U7YTE2	E-Value: 4.012e-103, PID: 43.70	(protein IQ-DOMAIN 31 isoform X2 {ECO:0000313\|RefS... [more]
A0A1U8Q610	E-Value: 6.021e-100, PID: 44.38	(protein IQ-DOMAIN 31-like {ECO:0000313\|RefSeq:XP_... [more]
A0A834Z5V8	E-Value: 4.747e-97, PID: 41.92	(Uncharacterized protein {ECO:0000313\|EMBL:KAF8399... [more]

Pages

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR peptide blastp to SwissProt and TrEMBL without DCAR	This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.