A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.

Resource Type: 
Publication
Publication Type: 
Journal Article
Title: 
A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.
Authors: 
Konieczny A, Ausubel FM
Series Name: 
The Plant journal : for cell and molecular biology
Journal Abbreviation: 
Plant J
Volume: 
4
Issue: 
2
Page Numbers: 
403-10
Publication Year: 
1993
Publication Date: 
1993 Aug
ISSN: 
0960-7412
PISSN: 
0960-7412
Cross Reference: 
PMIDLoading content
Citation: 
Konieczny A, Ausubel FM. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.. The Plant journal : for cell and molecular biology. 1993 Aug; 4(2):403-10.
Abstract: 

A set of mapping markers have been designed for Arabidopsis thaliana that correspond to DNA fragments amplified by the polymerase chain reaction (PCR). The ecotype of origin of these amplified fragments can be determined by cleavage with a restriction endonuclease. Specifically, 18 sets of PCR primers were synthesized, each of which amplifies a single mapped DNA sequence from the Columbia and Landsberg erecta ecotypes. Also identified was at least one restriction endonuclease for each of these PCR products that generates ecotype-specific digestion patterns. Using these co-dominant cleaved amplified polymorphic sequences (CAPS), an Arabidopsis gene can be unambiguously mapped to one of the 10 Arabidopsis chromosome arms in a single cross using a limited number of F2 progeny.

Publication Model: 
Print
Language: 
English
Language Abbr: 
eng
Journal Country: 
England