SAMD00518116

Resource Type: 
Biological Sample
Name: 
SAMD00518116
Description: 

Celery cultured in control medium for 7 days

Organism: 
Cross Reference: 
NCBI BioSampleLoading content
NCBI SRALoading content
Project: 
NameDescription
Transcriptome of celery (Apium graveolens L.) under limited calcium (Ca) supply was analyzed by RNA sequencing, with the aim of understanding the impacts of Ca deficiency on plant metabolism. Seeds of celery (cv. Cornell) were germinated on vermiculite in a culture room maintained at 25 degree C and 16/8 h light/dark cycle. Five weeks after germination, seedlings were transferred to a solution culture. The complete nutrient solution contained 1 mM Ca(NO3)2, 1 mM KCl, 0.5 mM MgSO4, 0.25 mM (NH4)2HPO4, 100 µM  Fe(III)EDTA, 46 µM B(OH)3, 9 µM MnCl2, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM (NH4)2Mo7O24. Two-month-old plants were then transferred to either -Ca or control medium. For -Ca treatment, Ca(NO3)2 was omitted from the solution and NO3- was supplemented as KNO3. The uppermost leaves were sampled at 4 and 7 day after starting the treatment. Total RNA was extracted using an RNA extraction kit (RBC Bioscience).
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Age: 
7 days after treatment
Cultivar: 
Cornell
Tissue: 
Young leaf
Treatment: 
supplied with complete nutrients for 7 days
Geo Loc Name: 
Japan
Sample Name: 
control_7d_rep1
Dev Stage: 
Young seedling
Biological Replicate: 
1
Growth Protocol: 
hydroponic culture