| Relationships |
|---|
| The mRNA, DCAR_011445, is a part of gene, DCAR_011445. |
| The genetic_marker, MK004345 K1789, is located in gene, DCAR_011445. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_011445 | DCAR_011445.mRNA | mRNA | DCARv2_Chr3:36060163..36070977 |
ATGGCGACACCTTCTTCTCTCTCCACATCTCTCTTGAAACCAGACCTCCT
TTTTCGAGGTTCCAGCAACTCCAGGAAGCTTCGATTCGTAAAGGATAGCA
ATCGCGCTTATAAATCGAGCTTGAGAGTGAAAATCAGGGCTGTGAAGAGT
GAAGAAGCTGTGATTGAAGGTGTTGGACTAAATGGAAACGGCAGTTATAA
GTATAATGGAAATGGAAATGGAAATGGAAGCTTGGTTAAATATGTTAATG
GAAATGGAGGCGTGGGGAGTAATGGAGAGGTTCCGAAACAGAAGAAGAAG
AAGAGTGTTGAGGAAATAGGGCAGGAGGATGCTTGGTTCAAGGAAAATAA
TCCTAGCCAGCCTCAGGTATACATATGCTTCTGCTATCTCTTGTGTTGAT
GCTTTATTAATTCTTTGTGTTTTCTTACTGTTATTCAAGTTGCAAAGCCA
GGTTCTACAACCTAAATTACTTGTACTGACTTAAAGTAAGGCTTCGTACA
CCATCACCCTCCCCTCACCGTGCTCCACAAGTTGGATACATTAATATGTT
TCGTTTCGTTTCGTTTGGTTTGGTTAGTAGCTCTGGTCTGTTTATCATCG
GAATGCTCCTAGTTCTCACGGTGAGATGAGGGTTATTGTTGTTGTATTGT
TTTTACTATTAGGCACTATTAGGTTGTGCTGATTTGGATGGAATAGAATG
AGGAGATAATGGAATGATAAATATAAAGAAGTTTTTTGGAGAAAGAAGAG
TGTATGAGACAATAAGGACTAAAATGACTATTAATTTCTTTTAATGTAGA
TTGTAGGGAAAATAATGAAGAAGTGGAATGAGCATTTCTTCCAGTTTCCA
TAGCATATAGGTTAGATTTCTAGTTCAAATAGTTTGGAATTGAATGATTG
AAGGAATGAAAATATAAAAATGATTCCCTAATCACTTCCAAGTTAGAGTA
CAAATTTCATTCCATTCTCCCCCATCCTATTTCATCCAAGTGAACGCAAC
CTTAGGGTGATAATTGTTTGAATATGATAATCCAGGTAAAAAAAGACCTA
CCTTATGTTGTGTTTGGATGGGATCAATGAATTCGGACATGAATAGAATT
TAAACTATTGTCATGTACAATTGTTCAAAATTTTCATTCCTTCCCTGTTT
CCATTACTTACAACCCTATACTATAAGTCACACCCCCAACTTAAGAAGGA
ATGCTCCATTTTCTCCTATACCCAATTTCTTCTCAATTCTCATCATAATC
ATTTTGCACTCGCTCATTATTTTTTCCAAGTTTCCCTCTATTTTCTTTTA
TTTTTACTCATTCTCATTCCATTCTCCCCAACCAAACACAACAGTAAGAT
TTTAGATAGACTAGTTATAAGTTGAGAGATCCTTAGAGAAGGGGGAGGGG
TAAGGCTTCTTATATATACATAGTGTAAGGTAACTTCTAGTGATTTATGG
CCACCAAAGGAATATTGGAGCACTATTTTTACCCTCAATTCCTAAAGTTT
ATTTTAGGAGGCATGTACAGCTAGTCTATTGGAGTTGCTTTAATATTATA
AAATGTTGATTCCGGGAGACGCTTGGCAGTGGTAGTTAACAAGAAAGTTA
AAACTTACAGGGTTTACATCATCAAGAAACTCCACGGTAAGGATTAGGAT
GTAGGAAATGATAAATTAATTAGGTTATCTGTTTGTCTAGTTATTATTCG
GCATGGAAACTCATGTTCGTTATTACTTGGCATAATGCAGAAGCTTTATT
TTTTTAAGCATATGGTTATGCAGGTGTCTGTTACCCCTGGAGGTCGTTGG
AGTAGATTCAAAACCTATTCAACTATACAAAGGACTTTGGAAATATGGGG
ATTTGTTTTGACTTTCGTCTTCAGAGTTTGGCTGGATAATCAGAAGTTCT
CTTATCGTGGTTAGTTACTTTTGCAAAACTGTATATCCTTAGAACATGCA
CAACAATTTTTTCTTTTTACTAAAATTAATCAGTTTCCTGTTATGGGGCC
AGAAAGTCACCAGTTCATCAATCATCATCTAGATGCTTGTTAGATGAGTT
ATCAATTTATTTGCCCGGTATAACTCAAAATCCCCCATATCTCTAACTAG
CATCTTTTGTATGGAAGTTTGATTATATTTTGGTTATATAATCTTCATGC
AGTATCTCACACAACGGGTTTAAATATTTTAAATCCAATAACAAACTTCT
TTACACTGTAATCTGCATTACAAACACAAAACTATTAACTTTTGTAAATT
AAAACCATGAACAAAATGTGTAGATATGATGTGATAATATTTTGTGCAAG
TTTAGAACTCTTGGAAATAAGTGCAGTGTTACATCCCTTTGTAATGTCTC
AAAGAATGTTTCGTGGTGTATATACTGTTATCTTCAAACTCATATATCAT
ACACAGGTGGAATGACTGAACCAAAGAAAGTGCAAAGACGGAAAGTTCTG
GCCAAATGGCTAAAAGAAAGTATATTGAGATTAGGTCCTACTTTCATCAA
AATTGGACAGCAGTTTTCTACAAGAGTGGATATACTAGCCCAAGAATATG
TGGATCAACTGTCAGAGCTTCAGGTCATTATTTGTTATATATAAGTTGTC
AAACTTTAAACCATTGCATGCGTCTTTGCACCCTCTTCTATTGTTGGATG
ACCATTTTCTCCTAGCAAGAAGGAACTAGTCTAACTTTGCATATCTGAGT
ATCTCTATTTTTGGAGTAGAGTCTGTTGGATCTTTCACAAGTAAAAGTTG
TTGATGACTAGACTCTGACAGATTATGAGCTCTTTCATAGTTTCACATAC
ATCATGTCCCTTGTTACTTGAACAGGACTATTGTTTGATCCTATACTTTT
ACATGGCTCTATAACATCACAAACATCACATGCTCTTTTCAGGCATGAAA
AAATGTGGTTGTAAATAATATATTCCTTTTTCTGTGTGATCATACAAAGG
GATCACAACACAGAAGTTACTCAAGTTGCTGTGTTTAACCCATATATTTT
ACTCCAAGGGGAGTATAATAGAGTGAAAGGGAGCTTCATCTCCGCGTCTG
AACTCCGATTTTAATTATATACAGAATTACCATATGTACTTGTGTTTTAG
CGAAATTATATAATTTTTATTGCATTTCCCCATCCATATACTTTCCTTTC
ACTTTCTTAATTTGTTCTGGTGCACCCAACCATCACAGGGCATTATTGTC
AAATATGTTCTTAATTATATTGATGATACATTTTCCCTTAAAAATGCACC
CTCACCACAGTTCCACCTCATCATTTACTTTCTCATACAATAAAGAGCAC
AAATGAGTTAATTTAATTTCTCAAGGTGAAAAGGGTGTATGGAAGCCATG
TACATATGGACAAATAACTTGTAATTGGAAATATCAGCGTCTTAACCGTG
TAAGTATTTTGGAAGTTTATTACCCACTGGTGTATTATGTCATTAGCAAG
GTTTGTGCCTGTCTAGATGCTTCATAAGGACCTGGGTTTCTTTGTAATAG
TGTCTTCTGTTCATCTTCTATATACTTTGTCCTACTAATCCAATTAAAGC
ACGAGGGAAGAAAGGGATTCTTCTGCCAATCTGGATTAGCTAATCAAATT
TTTGTTTTTTATAAAATTAGTCTTTGCTAAAACGACATGTATCTGCAAGT
TATATGTAGTATAGGTTAGTCTGTTATCTATGATATGTCCTTCGAGGATT
TAATTATGTTATCATTTCATGAAGGATCAAGTTCCTCCTTTCCCATCAGA
AACTGCAATTTCTATACTTGAAGAGGAACTTGGAGGGCCAGTCGATGATA
TTTTTGACCATTTTAGCTATGAGCCAATAGCTGCTGCTAGTCTGGGTAAA
CTTATGATCTCTTTAATCTCAATGTAACAATATTCATTCCAAGCATCCAT
TGATATATATTTGGCAAGTAAAATGGAGGAAGCTACTGTTATTTCTGTGC
AGGTCAGGTCCATCGTGCAAAATTAAAGGGTCGTGAAGTAGTATGCAAAG
TGCAAAGACCTGGTCTCAAGGATCTCTTTGACATTGATCTAAAAAACCTC
AGGGTAAGTTTCAGACAGGTTCTGGACTTCCGTGGTTTTTATTGAGGTGT
CAGGATCAGATTATAGAGATATAATGTATCAAGTGCTACTTGCTGAAATT
CACCACTTTTATAATATATCTGTCATTATCTGATGTTATAAATGAGTCAA
GTTATTCAGGCTTTCTGTAGCAACTAAATTCTAATATTGCATTACAAATC
TATGGAATTCTCAGGTCATAGCAGAATATCTCCAGAAGGTCGACCCTAAG
TCTGATGGGGCAAAAAGGGATTGGGTTGCTATCTATGATGAGTGTGCTAA
TGTCTTATATCAGGTATTGTTATCTTTTGTTCCACTAGTTGCATGGCTTT
CGCAGTTTTTTCACAAAGATAAAAAAAAAAAGAAGAAGAAATGAGGGATT
TTCTGTCGTAGAAAGGCAGCAACGAAACTAGAAAACTTGATCAAGACAGC
TGGAAGTTTCTTCGCACTCTCTATGTACATTTTGGTTTTGCTGAATCACT
TTCTACAAATTTGCATACTTTAGGCGCGGTTAAAGGACTTTAAAGAAGTT
CGAATGATTCAATATCAGATTCCAATCTATAGTATCATCTGCACAATTAT
GCTTGCTCTTATATCACGACAATAGTAATATTGTCATGCTGTTGCATGTT
TTAGAATGTTTCATCACGGGACAACCTGATGGTCTGAATGTCTAGGCCCA
TGACCTGATGGTAAAAGTATGAATAACCTACTTTCCTGTATTAGTGATTG
GAGACTAGAGACTAATAGAATTTATATTACTATTTACTACTAAAAAAAAA
GTAGTAAAAAAAATATTTCTGTTTGGTGCTGGTGGATCAAGATCCAAGGA
TATTGAAATCAATGATATCGGCACTCAGTGTTGTAGAATATTATATGTTT
GGACAGACATAATATGCAAAATGAAGGAGTGGTTATTGGAAACAAGCCGT
GACATTTTAATTCGCAAAAAGTAAAATAAGAAATAATCTGTGACGATCTG
TGATGACTGCATCGACCAATAGTAATAGTTTTTCCACAGAAGGAATACCG
GATAACAGCATATTTCCAATACAGTCAACACCAAGGAACATATAAAAGAA
ATTGTAAAGTCAAGTATGTGTTATATTACATTAACTTAATATCTGTTTCT
GAGTATATGTTAAGATCCAGTTATGTGTAGTACACAATTAGGCTCTGTTT
TTGTATTTAAGGTTTCTTTGGCCTTATTTGCCACAATGGAAAAACTTTTA
TATTGAAATTTTTATCACTGAGAAATGCTTCCAATTAACTTACAGAAATT
TAACCTCAAACGGAGTATTAAACCTAATTATTTCTAAGCATCCAGGGTTA
GTCCCAAGCACCAGGGAGCAGCAGCCCCATTGAATTTCGAGTATCAGTAT
TGGATGGTGCATAATGTATAATTGCTGTCCATGTCAGTTTGTAAGTTGTG
TTCTAGTTTGAAAATATATCGACACAGGTTCGATTTTCCATGACTACACT
ATGTTTTTCAATATGATTATCTTTTTCATTTGTCAATTTAGATTTTTATT
CGTTATTTATTTTTCATAATTACTCAGGTAATTCGCAATGTTATAATCGT
TCGATAAATTACATTAACAGTATAACAAAATTTTATTTTAATACGCTTTG
TTATTTATCAGTTACTTTGTTTGCCAAATTTTTTAATTTGTGACTAAAAC
ATTATAACGCTGATGACGTAGATGTAAAGTATAATCTTAATTATCTGTTA
TTAACTTGTTTGTACAATTAGAATTAATATAGTTAAATTATTGCTTGTGA
TAAGAAATTTGGTTGCCCTCAAGTCAATTTCGTGGGTCGCATCAGTATAT
CTGCACATAATCTCTCTGATAGTGCCGTGGGAAAGGTGCCCTGTCCTATA
TGCATATACACATTGATCAATGTTTCTTGACCTGCACAATCATTAGCTCT
AAATTGGTTCTCCTAGCGATTATACTTATGATCAAGACCTCCCATTGAAA
AAAAGATGCCTTCATGATTTTGTGTACTGTTAGCAATATATGACATGATC
TGTTATGCTCCCTTTCGTGAAGGTTTATGGTTGCTGTTTGTGTTTCTTTC
CTGAATTTCTAGGAGATTGATTACACAAAGGAAGCAGCTAATGCAGAATT
ATTTGCAAGTAACTTTAAAGATATGGATTACGTAAAAGTTCCTACAATAT
ACTGGGAATACACTACACCACAGGTTTAGACTTTTAATTCTCATTTTTAT
AACAATCTTAAATTTTAGATTCAATATTTTTCCATGCGTCTTTTAATTTG
TTTGATTAAGATATAGTTTTCATTTTCTAATGTGTAGGTTTTGACAATGG
AATATGTACCAGGGATCAAGATAAACAGGATACAAGCTTTAGATCAGTTG
GGTGTTGATCGCAAAAGGTAAACAGTGCTCAGTTATTTCTTTTAAAAAGT
ACGTATATCTGTCAGGTGGACTTTTCTGCTTGTTAACATATTATTTGACT
AATTCCTATGACCCATATAGTTCTGATGTTAACTTAATATTCTCCAGGTT
GGGCAGATATGCTGTTGAATCATACCTGGAACAGATTTTATCTCATGGTT
TTTTCCATGCTGACCCTGTGAGTTTATCAGTTCTTTTGTCTTTTAAATCA
GGTGCTATGTTTGTTTGTATTAGGTTATTCTAAGAGGCCAACCAATTGTT
GAATAAATATTCAGTCCACTGAAGAATGTTAGATCTTACCATATTAATTA
TTTATGAGCCTCTAAGAAGAATATGTTAAATAGCTCAAAAGAATTCTCGC
TGTTTGACTAGAATCTTGATCATTCTGCAGCATCCAGGTAATATTGCTGT
AGATGATGTCAATGGTGGGCGGCTAATATTTTACGATTTTGGAATGATGG
GAAGGTATACATATCTCTACACTTTAGGACCTCTCAACTCAGAAAAGCTA
TCTTGTATTATTACTGTATATGTGTGTATTTCGTCTTAAATGTTAAGTTT
TGACTGCTTTCAGTATAAGTCAAAATATTAGAGAGGGCCTGTTGGAGGTA
TTCTATGGAGTATATGAAAAGGATCCAAACAAGGTAATTACTATTTACTA
CACTCTAATATCTTTTTTGTGATTATGATCCAAGTTTAGACAGTGTCACA
AACTTATGATCAACCAATGATTATTTTAGTTGTAATTGTATAGAGTCCAT
AACTAGAACAGGTAGTTAGTGCAACATTATTTGATCTTTCAGTTGATCCC
TGGTTTAATCTGATTGATTCTTGCCATTTTTAGATTATTGCATCACTATT
AAAATTCATACTTTACTGCAGGTTCTTGACGCGATGGTTCAAATGGGTGT
TCTTGTTCCTACTGGGGACATGACTGCTGTTAGACGAACTGCACAATTTT
TTTTAGTTAGGTATGATTTACCTACAGATTCTTTCACCAGCCTTCTTTTG
TTTTCTAAAATTTATATAGGCAATCTATATAATCATTTGAAGAAATTTAT
TAGTAGGACTGTAGGAAAGTGAAAGATTATGTTCTTTCTGAAGATGCGAT
GTGATGTCAAAGTGCACATCCCACGTGCTTCCTGCCTCGTGCCTATTATT
ATTAAATGCTAATGGTTTGATATGGAAAGTTAAAGAGTATGTACGGCTAA
TATGTTATAAAAATTGGAACGACAAAATCTTAAATGTTAAACGGTGTGAT
ACGGATGCAGTGCTGGCCTTAAGAGAAGCATCTGATACATCATACATGTA
ACTCGGAACTCAGAAACATGCCATGCCGTGGTCTTGAGACTTAGTTTTCT
TAAGACAGTCATACTAGTTGCATAATTTGGATATATTTAAATTGAATTAA
AGAACAGCCAGAATATATAACTAATGGCTTGATTAATCTTTTGTAGATGC
ATCTGACAAAAATATTGTTTTGTAAATGCATGACAGGACTTGGGTAAATA
TAGAAGGAGAATTAAGAGAATAAGAGAACACTTCAAAAAGATTATTTCAA
GGATGAAAACCCTTCAACAATTTTTCATCCTTTAGCTGATTACAAAAAGT
GAATTTTTTTATTATAACAATTTGGGTATAAATATAAATATTTAGGAATA
TGTTTTGAAAGCTTGGTTAGAGTATTTAGCACTTCTGTATAATATTTTTG
AAAAATAATTAATAATGATTGAAATTTTGATTAAAGAAGAAAATTATAAT
AATTGAAATGTGACCTTAGTTATTTATGTATAGGAATAAAGTTAAGTGTG
ATTTTTAATTAAATTCCCTTTCTGAATATTGTTTTTGTCACTATGGTTCT
CCCTTGAGCCAGACCGTATATACAATTTAATTATATTAAGTTGAAGACTG
AAGTAGAAATTTATAAGAAGTTTCCCGATAATTTATGAAAGTTATATTTC
TGTAAATATACAAATTGTAGTTTTTCCTTAGCATTTTCTCCGTTTAAAGA
AAGCTATTGTGGACTTGTGGCGACATTGATACTACTTTCAGGGTCAGGAA
CTGAACCTGGAAAACTGCATACGGCTCCAAACATAACATTAATTGATTAA
TTTGCAGCTTTGAGGAGCGCCTTGCAGCGCAAAGAAAAGAGAGGGAATTG
GCAACAGCTGAGCTTGGGTTTAAAAAGCCATTGAGCAAGGAAGAAAAAAT
AGAAAAGAAAAAGCAAAGACTAGCTGCAATAGGTACTATATTATATATTC
TAATACTGCTTCTGTGATGCACTCTTAATTTAATTTGAGTTGTGTTTTTT
TTTTCCGTAGGAACAAATAAATATTTGTAAGGTTTATGTATCTAGTCAAA
ACTCCGTCCATCCTCCAACAAACATCCCAATTTTTACGTAATTTGAAGTG
TAGAAAGAGATGCCTGTAAACAATATGTTTACATATATTTTGCTGTCATA
AGAACCAAAACTATTGCAGGAAAAAACAAAAACACGAAATATTAAATAAT
TTAAAAGCATATGTTATATTATTTAGACGTTTACTGTGGGACGGAGGGAG
TATTTATACATTTGTGTGTGTGTTGTTACTTGGTATATCAAAGGACATTA
ACCATGATCACAGTTGTAAGCTATGTTTTTTATTTGAATGGAAATAATGA
AATATTATGTTTCCCCAAATTTCTTTGATATTATATTCACATTCAGTAAA
AGTTTATCATTCTTTGATATTGTATTCACATTCAGTAAAGGTTTATGATA
CATGTATAGGTGCACTCAACGTGTCCGAGCCTGTCTTTGGATTAAAACAA
AGTGTATGATATTGTGATCATATTTCCATGACTTGTCATACTAACATGAG
ATTTTTTGTAACACTAGATATAGAGGTGATAAACAAACTTGGGAATTTCG
GGCCTGATGTCATATTTATTCCTTACACTTCGCTTTCGTTAATTACAATA
ATTTCTATTTGTTGCTCATCCAATAAGTCTGGATTTAAACCAACAACTAT
GCCAATATGGGTTACATGTCCTTTTATTTTAATATACTTGGAAAAATAGT
GAAGTACTTGCATAATTTTGCTCAAAAAATACTATCTTTAAGTAGTCATC
AATTTCTATTGGCAGGGGAAGACCTATTAGCCATTGCTGCAGATCAACCA
TTCCGTTTTCCAGCCACGTTTACTTTTGTTGTTAGAGCATTTTCAGGTTT
CTTATTGTTTTTATTTCAACTAGTATTTTCTTAATACTTTTATCTTTTGC
CAAATAGTAACTTCCTGTATATACACAGTGTTAGACGGAATTGGCAAGGG
ACTTGACCCTAGATTTGACATCACTGAGATAGCAAAACCGTGAGTGCAGT
TTAATCCTTTTGGTGCAGTTTTATCATATCCGACCAGAATTTGGAGCTAG
CTGTCCTGTTTATTTCTGACATGTATGATACTTACCATTTAGATATGCAC
TGGAGCTGCTTAAATTTCGTGAAGCTGGTGTTGAAGTCATCATAAAGGCA
ATTTATCCTTACTTTCTATATTTTACCTGAGCCTGGACCTAAAAGTTCAA
TCTATATAATGGTAAATCGTGCAATTGTTCAGGACTTCAAAAAGAAATGG
GATCGACAGTCTCAGGCATTTTATAACTTATTTAGACAGGCTGATAGAGT
TGAAAAGCTTGCTCAGACAATTGAACGTTTGGTACGTATCATGTACTTAA
GAAGATTCCTCCATGCTCCACTAGTCATATTAATCTCTGCATACACTATT
ACAGGAGCAAGGTGATTTGAAGCTCCGAGTTCGAGCTCTGGAATCTGAGA
GGGCCTTCCAGCGTGTTGCTACTGTTCAGAAAACTATTGGAAGTGTGAGT
TCATCGGGTTGGCTATAATTTAGTTATATAATTACACAGTCCGTGATGAT
GATTAAGACTTTAACTTACTATTGTATCTGAACCGAGTAGGCATCAATTT
TAATTGGCATTATGTCACATCATACGAAGAAGTTTCAATTTCTAACATTA
GTGATCATCATTTATGTTTTCATCACATTTGACTAATTACAGGCAGTAGC
TGCTGGAAGCCTGATAAACCTAGCAACAATTTTGCATATCAACTTAATTC
GGGTAGATACTATTTTTTTAGCAAAAAATTATTATTATTCTCATTTTCTG
GTCTTCATTGCCAAAAGTATTCAATACAGACTTTTTAAATATAACAGGGC
CCTGCAACTGTGGCATATTTTTTCTGTGCCTATTTTGGCTTGCAAGTCCT
CATTGGCCTGTTGAAGGTTAAAAAGTTTGATCAGCGAGAGATGCTGATAA
CAGGAACTGCCTAA