| NCBI Gene |  |
|---|
| Relationships |
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| The mRNA, XM_017367433.1, is a part of gene, LOC108199559. |
| The genetic_marker, MK010972 8_22427876, is located in gene, LOC108199559. |
| The genetic_marker, MK009832 8_22428017, is located in gene, LOC108199559. |
| The genetic_marker, MK009831 8_22428053, is located in gene, LOC108199559. |
| The mRNA, XM_017367432.1, is a part of gene, LOC108199559. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
The RefSeq genome records for Daucus carota subsp. sativus were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results. View the full report at https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Daucus_carota_subsp._sativus/100/ Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| XM_017367432.1 | XM_017367432.1 | mRNA | DCARv2_Chr8:22418323..22428409 |
| XM_017367433.1 | XM_017367433.1 | mRNA | DCARv2_Chr8:22419750..22428410 |
GTCACACTTCAAGTGGCGCTAAACGAGTGGCCCGGAAATCCAGCCAAAAT
TAAACCGCAGATTCAATAATCTCTCTTCTACTCTCATAATCTCTCTTTCT
CTCTCTCGCGCTCTCTCTCTCTCTCTCTCAGTCAGCATGATTCAACACTC
TCTCCCACAATCATAAGTCATAACTAGCTTCATTGCCCAAATATTCTTCC
ATTCTAGGGTTCCGTGATCAAGCATGGCGCAGCAACTGAAGCGCGGGATC
TCCCGCCAATTCTCCACGGGCTCGTTGAAGAACGTGATGAGGCTGAGCTT
CAAGCGGCAGAACTCGCTGGATCCTCGCGTCAGGACGCTGCGATTCAGCT
TCGGCCGCCAGTCGTCGCTGGATCCGATTCGGAGGACCGGAGTCGACGAC
GAGTTGAGCGTGCCGGAGAATCTCGATTCCACGATGCAGCTGTTGTTTAT
GGCCAGTAGAGGTGACGTGGAAGGCGTCTTGGAGTTGCTTGACGACGGCG
TCGATGTTAATAGTATCGATTTGGACGGCCGGACGGCGTTGCATATCGCT
GCTTGTGAAGGTCATAATCATGTCGTTGAAATCCTGCTCTCTCGCAAAGC
AAATATCGATGCTCGTGATCGTTGGGGCAGTACGGTAATTTTTTCTTCTT
CTTTTTCTCTATCAATTCACAATTTTGTTTTATTTTAATTTTTAATTTTC
TTAGCTACTGATATGAATACAGGCGGCAGCTGATGCAAAGTACTATGGGA
ACGTTGAGGTTTACAATATCTTGAAAGCACGCGGAGCGAGAACTCCGGTA
TAATAACACTTTCTTTATATCACGCAATTGCTTATTGCTTTGAAGATAAT
CATCAATTATTTTGTAATTTTCGTAGAGAACCAGGAAAACTCCGATGACA
GTTGCAAATCCTCGAGAAGTGCCGGAGTATGAGCTCAATCCCCTGGAGCT
TCAGGTTCGCAAGAGTGATGGTATCTCTAAGGTATGTTTCCTCTACTTCG
TCTAGAAGTATTGTTGTCTTGTCAACACCATGAATCCTCTAGACCAAATT
TGTGCTAGTTTCCGACTTTCCGTGATTGTAGTACTGGTTTCAGTGCCAGC
ATTATGTTTCCCCCTCTGTTTTGTGCAGTTGTCATACTGGATGTGTATGC
TCACCTTTTTGTGCTGTGCAATCTTTTATTCTTAGCATTTAACTTTCATA
GCCAACATCACTTGACTTGGATTAGATAGAATGTATGCAGTACTGAGAGA
TTTCACTTATGAAGTCCTCTTAGTAACTTAAAAACTGCAAAGAATTTTTT
CTAGTGTACTGGCGATCCGTGATGGATATCTTGAACTTAATTATATTTGC
TAAAAACCCAGATGCTTCACTTATATAAGTTCTGACTTGTAAGAGTCATT
AATAACCGAGTACACTTGTCAACTGCCAGCATCGGTAATAACTAGTTTGT
GGTATATTCTTATATGGTTTTCCATATTAATTTGTTACGTCTGCGTTCAT
TTTTTATGTATACTAATTGTTGATCATGTCATAACTGGTTTGCATATTTT
TATATATTGTCAATTTTGTGCAACTTCAGTCCCATCATGAATAACATAAT
TGTCAGATGTGTCTGTTTGGTGCATATTATGGCTTAAATATATTGCATAC
ACTGATTCTTGTCACCAAAACACATGATAAAATAGATAATATTGTCATCC
ATTCATCCTCCTATTGTTGCACCATGCTTCCCCCTAGCATCCAGTTATTC
CTCTGTATTTTGCATGGCCTACTGCAACCAGTACAACTATATAACTAGAT
ATTTGTCAAATTCCAGGGAACATACCAGCTTGCCAAATGGAATGGCACAA
AAGTCTCTGTAAAGATACTTGACAAAGAGAGCTATTCAGACCCTGAATGC
ATGTTAGTGTCTTTTTTCTCTCTTCTCAATTATTCATTTAAATAAAAACA
AGTCATTTTCTTACATTTGTGACCACACTACTTAAAAGGGAAAAACGTCC
TTATTGGCAATTCAAGATATTAGAGTATTACACTATATTTATGCTTTTTC
TGCTTGTAGAATTCTTCTCTTTGTTTTTTGCTAATTAGCATGTGTTGATG
TATGCATATCTGATGTGATAAATTGTTGGGAACAAATGATTCTTCTTCTT
AGTCATGTCTCCTCCAAATTGACTACATTTATTTTACTTTCTCATCACAC
ACATTACGATCTGGTTCTGAAATTGAGATATCATATTTGGAAAAGAAACT
TTTATACTTAGAAATGATAAATGTGTGATTCTCTGCTTTGAATTGGCAAT
TGCACCAGCCTGTTCTTCAGCAATATTCAATACGCTCCATTTAAACAATA
CTGCATGATATTTTTACATGAAAATCTTTTGATGTTATATTTACTCTTAA
AAATGTACAAGCCAAGGCTTTCAACCTACAAATCATATTGCAAGAAATCC
AAGGATTTTGGGCCTCCGAAACTACCTTTAGAAAGAGAAGAGCAAGATGC
TATGTGCTGTCTCTAGATACAAAATCTATGGTAAAGCTTTGATACTTATA
AAGATTAATTTGATAACATACTCTCCTCAGTTTGTTATGTCCCTGTTGAG
CGTTTCTTCTCCCATGGCCCAGTAGCTACCAATATGTACAGATTCCAATC
CACCCGCCTTTATCCAACCTTCTACCTCAAACAGCACTCCTCTTACTAGG
ACAATCCCCTAATGGCTTGTTTGGGAACTTGAAATTCATTTGAAGATTGG
ATTTGGATAACAAAGCAATTTTAATTTGATTGAATTCCACGTCTTACGAA
TTCAAGCATAAAATTATAAATTTTGAAATGACTACTTAATTCATGTCATC
TGAAATCCTTTATCTGAAATGAATCTCATGTTCCTAAACACAATCGAAAA
ACATATATGAGGGCAATGCATAACCGACCTTATTCTTGTAGGATTCAAGT
TCTAATTAATAGTCCTACTTTACTCGTTTTGCTACCATACTTTATTTTAA
TGTGCCCAAGCAGTAGAAGACTCGTTGTACTAAGTATAAAGGTTCTCATC
TGTATACCATATAATATGTCTCAAGTCCAAACAACTCTGCCCCTTGACAT
CTCACTTTACCTATACATGCTCTATAGCTTGTCCACCATGTAGTACATGC
TGTTTCGATTAGTATCCTCGCTGATTCCGAGGTATAAAGAAAATTTTAAT
TACTATAAAATTACCTGTTCAGTAAGCATCTCAATGGCCTAAATGCAATA
CACTGTTGGTACTCCTGGTAATAGAAGGAAGACTGTAGATACACATGTTT
AAATATATATTGTATAGTTCTACTATTCAAGCTTATACGTATTTTTTGCA
ACAATTAGTAGTCCTTTGTTAACATGTTAATACCCAGAAACTATAGTTTC
CTGGAAAGTACACAATGGTCTACTTGTTTATTACAGAAACAGAGGAACTA
TAACTTTCTTGGAAATTCGGATACCCAGTTAACCTACGAAGTTTCTTACC
AACCCCCTCTTGCTCAGTTGCACCTCCCATGAATTTAAATTCCTGCATGT
CAACTGAACACATTTTTTGTCTTCCTAGGAAGTTTTGAACGAATTAGTTT
CAAAGGAAGGTAATACCCGTATGTAGTCAAACAACCACTTAGATTTTGCG
GGAGAAAATTAGATTGAATAAAGCTTCTTCTGAGATATTGGTGACCTCTA
GTTTATGCTTTGAATTCGTAAGACCACAAAATTAGAAACTGTAGCCATCC
CTTATTCTGCGAGGAGAATGTAAGGAACACTAACTTTTGGTTATACAATG
TACTAGAATACTATTATTGCTCTCATTTTATGTTATAGACCTCGGCTCCA
GCGATACACGCACATTACTATCTTCAATTTTGATAGCTTGAACGGCTTTA
AAAAGGTGTTATATGAAGAGATCACCAGCCTCTTTATTGGTAGTGAAATG
CTTATCTTTATAATTAGTAATGTGATAGAGGTGAACCTGTGCTTGTAGAT
ATCAAATTTTGTTTCTGCACATCAAGTTGATCATTTAGGAACAGAGATTT
ATATCTCTACATTTGCTTTGTAAATAATGATTGTACCATAGAAAATAATT
TTCTTATCTGATGTTTTAGTGTTTATGTGACCAACTCTATATCTCCTTAC
GTGCAGAAATGCTTTCAAAAATGAGTTAACATTGTTAGAAAAGGTTAGGC
ATCCAAATGTGATTCAGTTTGTTGGAGCTGTCACCCAGAATGTTCCCATG
ATGATCGTTTCAGAGTATCATACAAGGGTAAGTGGGTCTGTCTAACTATA
CACTTTTAAGGATAATAGACTTTAATTTATATCCCCAGTGGACGTTAAAA
GAGCTGAATATGTATAGTGAACAGCAGGGATGACAGCCCTCATATATTGG
AAATTTAATGTGTTGAATTGAATTCAATACACTTAAAATTTTAAGGCTGA
TGGACACAATTATAAACACCTACTTCCACATTTCAATGAATTTTTTATTT
TAGAAGTACGAATGACTACACTAATTATTGAATTCTATTATAATCAAAAG
ATTAAGAAATATACACACCCAGATGTTTATGCTAACTTTCTTCTAATGTT
ATCTAGTACAAGTTCACGACATAAAGATGGACAAAGCATTACCCCACTAA
TCTGGACTACATAACATGAATATAGTTGGTGAAAGTTATCAGTTTGTTCA
AAGTATTATACTTCCTTGTACACAAGCTACAATGTGACAATATATGTCAT
TTTTTTCTTAGAAATATATCATCTTGATTATTGTAACATGTATAAATATT
GTTTTAACCCGACGGCGAATTTTCAGGGTGACTTGGAAACTTATCTTCAT
AAAAAGGGGCGCCTTCCTCTATCAAAAACTCTCAGATTCGCTCTTGATAT
TGCTAGGCAAGTAAAAATAATGATTCATATACATTTAAGTTTTTTGTAAC
ATCATTGTATGGATCAATTTTTCCTTGTGCTCTTTATCACTATGTGTTAC
TCCTATATTACCTTCTATCCTATATACAATAGTTTCTGTTTCTGCTGGTC
TTTTAAGGTTCTATTATGGGCTTCCTATATTTCAATTTCTAGTATTTGAT
CTTACCTTTTTTCTTGTTGATCAGGGGCATGAATTATCTTCATGAATGTA
AACCAGATCCAGTCATTCATTGCGATTTAAAGCCAAAGTAATGAACTAAA
TGCATTATTTTGACATTTATAGAATTTTTAAGTGAGTAATGTTGTTAAGA
TGGACTGCACTTAATTTGAGAACTTTCACTAAGGTGCCAGAAATATATTG
CTGGATAGTGGAGGTCAATTGAAGATTGCAGGATTCGGTGTATTGAGGAT
GTCAACAATATCTCCAGACAGAGCAAAACTATTGCATCCAGGGATCATTG
ATCATTCAAGTAAGGTGTTTTTATTATTGAGATAGGGTAATCTACTGGAG
AAACAATTTGGTGCAATTGAAATCAAATGCGGTACATGATTAGGGTGAAG
TATTGTAAGATGTATGGCATGGTAGTATCTATAGCTTGTAGTGATCAATC
TTAAAATCAGAAGAGAAGTTATATAGTCACTTGAGAGTTATGAACATGAT
TTGAAGTGCAAACTTCCAAGTTGAAATCATTGGACACCATTTTCCCAGTG
CACTTTCCCACAGATGTCATTTCGTATCACATAATGGTTTGTATTAATCA
TTGAATGTACAACTTATAAATATATGATGGCACCATTATTGAAAATTATG
GAACGTTCCTCTTCATCTTTTAGTAATGCAACTTTAATAAATTACTGCAG
GTTTGTGCTAACAGCTAAGACTTTGCTCCTAAGTTTGCGGTTAGAGAAGA
GAATTAGAAATAAGAGTGGGGAGAGGATCAATAGAATTTATATAGGGTGC
TCCCAAGATTTTTAGGAGAAGATGGAAGTACTAATAAAAATGTAAGAGGA
TCTCTTTTAAATTATCCCAAAAATGGGAGGAAAATTTTAGGGAGACTTTG
TTGATAGTTTTCTCTTCTCTTCTAAAACAACTTAGCAACATGACTATGAG
AGAAAATTTACATGACTTCCTCTGCCCTTCTCTCTCATTTTTTTAACCTG
GGAGAGAACAGTGTGAGGCAGGTTACTACATTAAATGGAAAGTTTGGATT
TTTTTTTAATTTAAGTTATAACATATTTTGGACTTTGTATTCCTATTGCG
CCAGCTGGGAGGAGAGCCATTGATACATCACCTGCTATTGAGATAGGTAA
AGAAGGTGGTTAACATGGCACAATATCTGAAAGTCAATTGATCCTAGTAG
GCCAAGCAAGGATTTTGTACATACCAGAGTCCCACTATGTTATATAGCCT
AGCTGAAGAAAACCATTCTTTTCACGAAAACCTGAAACTTTCATTCTTAT
CCTTTGGATTGGATTATATTTATATAACTATTTTTGTATATTTTATAATT
TATGCTAAAATAAAGGATATATATTTTATAATTTATGCTAAAGTAAAGGA
AATAGATTGCCATTAAGTTTTATTATTTAATCTACTAGACCACAGCATTG
TATTAAATTTATGCAGAACCTTCATAAATCTATTGTAAAACCCTAATTAA
ATCTCGCAGAACCATGATAAAAATAATTTAAGATTGCAAAACTATATTAT
AAAAAAGTTTCAGATCCAAAAAATATGTATATAATGTTGTGAAGCTATAA
AATTTGCAGAATTATAATTAAAAAATCTATTACACACTAGGTTTAAACTT
TATTTTAATACAATAGTTATATAAAAATGTTACTAGTTTCGGGTTTGTAG
TCTAAAAGAGTTTTCACCAGAGTTTTACTCATGTTATATATGTGTGTGTG
TATTTACATAGTAAGATATACCCAAGAACCGCACCCATAAGTGAATGCAA
AGCAAATAATATGTTTTGTCTATATAGCATGCAGCCTGACTTATTATTAT
GTTGTTGCTTGTAGATTTATATGTGGCACCTGAGATCTACAATGGTAACA
TATTTGAGAAACGAGCAGATGTTTACTCTTTTGCTCTTATCATTTATGAG
GTATAACTTCTATTCCTTTCTCATTCACTTCATTCTTACTGCAGTATTAT
TTCCCTAGAATTTTTTATATATTCATTATACGTGGATCCCTGTCACTGTC
AGGTGCATACTGTCTTGCAAACATTTTTTTTGTGTGTGTGTTAGTTCGAT
TATTAATTAGCCAAAATCATTAGCTTAATCGAAAATGTTTTTGCTACAGT
TTGTTGTGACATGAAGCTCAACCTCTGTTGAAAATACCTGATTCTTTTCT
GTCATGGTTTCGCGACCCATTCTTTTTTCTGGAATATTAATGCTTTCTTA
AGATTATACAATTAAAAACTATAAGACTCTGGAGTAATTTCAATCACATG
ATATGGTGATATGATTCAGATGATGGAAGGAGTCCAACCATTTCATCCCA
AGCCTCCACAAGAAGCTGTGAGGTTAATGTGTGTGGATGGAAAAAGACCC
ACAATTAAGCAAAAATCAAAATCTTATCCTCCGGAGCTAAAAGAGTAAGC
TACTTTCTATGTAATGTCTACATCTTGAAAGTCAAGTAATAAAGTATGTT
GTCTTGGTAACAAAATGCCATTGCTTAATTATGCTAGCTCAAACCTTAAA
TGTCAAAAACGTTGTGCCAGCAGTAATATTCCGTATTCTGTTTTTTTTTA
AGACTTTTTGATCATTATGAAAATCATATTGTGGCATTAGAGGGAAATTG
GATTCTTTAAAGTAGAATGATGGTAGACGGTGAATTGTTTGGCATGGTTT
TAATACGATGTTTATGTTTTCCCTTTATTTCAAATTTGGAATCGTGTAAC
TACTACTATTTCTTTGGTTCCTACTTCTTAATGTCTTAATGTCACAAAGA
TGTCAATTCAGTATACATCTCAGCTAAACCCATTGTCAAACATCATTCAG
TTTATGACTTGGTGAAATTAGTTTTACTAGTAATAATGACGTCAATAAAA
GATATTTAAGTTACTTGATTGGCCCTTCTATCTATATGTTAGGTTTAGCC
GGAAAAAAATGACCCTTACAAATAAATGGATAGGTAGCTTGTGGAACTTA
ATGAAAATCACTCAAACACATAGATTTAGCCAAGGAAAATAAATAATCAC
ATTTGTTTAAAACCATTTAACCATGCAAACAACAAAAGATGGAGTTTAAA
AAAAATATATAGATAACTAAAGCTGAATAGCTCTTTTGACTAGTACTCCT
TTAAACATAAGGCAACTGCAAGCTTGGGAACACCTGATTAGTTCAGGAAA
ATGGTCGGTACCCTTTTTATGCTGAGTTGTTTGTGTGCACATTTTTAGGA
TTGCTAGTAATTACTTTTATAATTTTATTTGATGGCATATTATCATGAGC
ATATGGTATTTATTTGAAGATATGCAAGACTAATTCTTCATTTCTTAGTA
TCTGCATTGCCTGCATTGCGTATCTTTTGGGAGTTGTATTGTCTTCTCAT
TTAAATGTATTTTCACGAGCAATGTAGATTAATCGAGGAATGCTGGAATC
CCCAATATGATTTAAGGCCTACCTTCTCAGATGTTATTTCAAAGTTGGAC
AAAGTAATATTGAACTGCAGCAAACAAGGATGGAAGGACACCTTCAAACT
CCCTTGGTAAACACATGATCTTTTTTTTTCTGTATTGTTACTTATTAGTT
GGTGAACCACTTATTAATGGATGAATCACAAAACAGGGGCCAGAAGTTGC
ATGGTAATAAATATAATAAAGTCACACGAAAAAAAAAGACATAAACAGTT
ATATTCAAGTTGGTAACCTAAAGATTTAGGCAAGTTGTATGTGTGATCTA
TAAAATGATAGTTAATCTTATATTTAGCTCTTGAAAGTGGTCTGTGATGT
ACAGATTTGTCATATTTTACAATCACAGCAATTTGGAATCACCTCCAGCT
ATCGGGATTTGTTTGCATGTTGGACAATGGAAAAACAGAAATTCTGTGTT
TGAGTGGTAATGCTTGCCTGTAGGCAGCAGCTTTCGAATATCTATTAATT
TGATAGATATAACAAGGTGTTTATTGCCAAAAGAACCATAAACAAAAAAC
TACAACTTGATTGCTATTTAATAACAACAGCAACACATAAAGTGATAAAA
AGAGTGGCATTCAAGTAAGAATACTTGTGTCAATTGGTAGGACACAAGGG
AAAGCTTTGAAAACTATTGCAAGTTTGCAGGGAATAAAAAACTGTGTGAG
CAGAAAATAATACATACTAGTAAATGTGTATATAGCAACTTCATATAATT
GTTAGCTTTTGTACATGGTAACCCTGTTTTAGCCACATTCTCAGTGTCTC
ATGTGAACTTAGTACACTCTATTTGCAAATCCTGTAAAACATTAGAATTG
TATCTTGGGCAAAACCTGTGCGCACCGTTTTTATGTCAATTTTCAACTTC
CAATTTCCATTTTCCAATTTTCTTGTCATTTTTATTTACTTCTTTTCAAG
TATCATCTTCGATGACCTTGTTAATTTGTAAAACTATATATTGTTCACCG
GTGTACGCGGATGTTATTGTTCCATAGGATTGATACTGAACGAGAAACTA
ATATATCAATTTGTATTGTCCTGCAATCTGCATCTAGGAAAAGTGAGCCA
CACTATATGTGTTTGTTACTATCAAACTAATGCAGCTTTGGCTTGTATTA
AGTAATCCGAATCTGTGTATCCTTTTACAGTTTTCATCTTTCGACAAGCA
CACCAATCAAATTAGCTGACTATGAAAACACCAATCATTTTGTTGTAGGT
TATGAAAGATGCAACACAATGTTGGAAGAAAAAGAGTGGTTCTGTTGTAC
AGCTTCATGTTTCATGTTTTTTTGATTGAGAAGGCCAATGAGAAGGCCAA
TAAGGGATTTCGGAACCTGATACAATCTTGGAGAGCTAGTCTTTATGATC
TTGGGACCAAAATATGGTTCCTTTGTTGTCAATTTTCCCTGGGAGCTGGA
ACAGATCTGTATGTATAGAATGTGTGCACAAATTTTCATTTTCAAGGAAT
TAACAATAGAGAAAATAGTTTTGCTTTCTTCCCTTTT