| Relationships |
|---|
| The mRNA, DCAR_014360, is a part of gene, DCAR_014360. |
| The genetic_marker, MK009568 4_22514678, is located in gene, DCAR_014360. |
| The genetic_marker, MK009569 4_22514685, is located in gene, DCAR_014360. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_014360 | DCAR_014360.mRNA | mRNA | DCARv2_Chr4:22512129..22522283 |
ATGGCGAGCCGCTCAGACAAATCATCAAAGCGACGCAAGAAGAAGCGCGT
GGACGACACTCTAACCACATCCTCCTCATCGTCATCGGAATCTTCGAGTT
CCGACAGTGAAGACAACGGAAAACGATCCCGCAGGCGGCGAAAACACAGC
AAGCGTCACCGGAGCAGAGAGAGGAGCAGTTCGAGGAGACACAGAGATGA
TAAGAGGGAGAAACGGAGGAAGCATCGTCGGAGAGAGAAGAAGAATGCGT
CTGAGTCGGAGTCCGAGTCAGGTAGTGAGTCGGAACGAGTTAGGGATAAG
CCCGAGGATGTGATTAGGTTCATTTTGAACGAGTTCTCTGGCGTTGCTGC
CGATTTGGAGCAGGTATATTGATGATCTCGATAAATTACAAATTAATTTT
TATAGTCTCGGGGTCATTAATTTGTAGATAAATGAAAGTTTCCAGTCCCG
AGGTTATTAATTTGTAGATAAATGAAATTTTCCGGTCAGGATAGATAGAC
TGCAGTAGGCATGTGTTCGATAAAAGTTATAAACTAATCGAAATAACCAA
TTATTTAAGATCCATTTGCCCAAGTGATTCTTCTCGATGTTTAAGATAAG
ATGGTTTTAGAACTGTAGATAACGAACCAGACTGTAAGCTGGAAGGTGCT
GTGCAATTGCATCGAATTTAGTAATTAATACATTGCATAAGCTTTCACTT
TTAGGGTTGCTATATAACATAAAGTTTGTTAATGATTTACAAGTTCTTTT
ATATGTTATGAACTATTAGAATTAAACAGGGAGAATGCACAGTTTTCAAG
AAGTGCAGTCGCTGATATTTTCTCAGGGGTTATACTTTACACAGCTTTTC
CCTCTACCTTGCTCTCAATTTATTGATTCTAAGGTCTAAACTTTGCTGTA
GAGTGGAGAACTTAAAAGGTGTTGCTTCTTTTTGAAAAATAATTTATGAT
TTAGGTTTGCAAGAATCCTATTATAATTTCAGGTCTAAATTTAAAAAGAT
TTTAATATTTCAAGAATTTTAATAGCAGATTTTTCTGCCATCCCCTTTAC
CTTGTTGGCTTGTCTTCACTTATTCGAATCTAAGGCCTAAAACTTTGCTG
AGAAGTAAAAGGTGTTACCTTTTTTTATTTTATTTTACCATCCAAGGTGA
TGGATACAGTATTCCTGCACTTGCAAAATAGCTAGTCTACTACAGTCCTT
AGTGATGCATGAGTATAAGTTCTGGTGGTGGTATCAAACTATTTAATGCC
CTTCTAAACTTTCTAAGTTCATTATTAGACGACATTTTGATTTGGTGAAG
GTCAATGCGCCTCAAAAGCTGTAGATGACAATTTTATAAAACCACGGCCT
GCCGAGGAGGTTTTTTCCCCTTTTTTTATTTTTATAGATTCGGCTTGAGT
AAGCGAATTCCGTTTATGTTATTACATGGCTGTCCTCTTTAAACACTCAG
TGTCAACATAAGCATCATTGCTTCATGTAAAAAATGATAACATATTATAA
ATCACATGCTGGAAAATAGTGAAGTATTTCTGACAAAAAAGTTTCTCTTG
TAGCTTCTGAAAATGATTGATGATGGACAAGCTGTTGATATAAGTGGACT
GTCAGAAAAATCTTTGGTCAAGCATCTTAGAAAGTTGTTTTTATCATTAG
ATCTCAAGGAGAGTCATGAGTTGGTCTTTCTCTTGCCTCCGAATGTTTGT
CCCACACTAAAGGTTGTAGGATCCATCATACACCCTAATGTGGAACCTCC
TGTGCAAAATATCGATAATCCCGCACCTGAAATTGATGTACAAGAAGTGC
CATCAGATGTAAATACTAGACATATGCCTGAAGATTTTAACATGAAGTTG
CCATCTCCAAGGCAAGATGTTGCTGGTCCTAAAAGGAGGTATTCGCATCA
GTTTTTTTTTTTTTTTTTAGTCTTATTATAGTACATTAACTTCTCATCAT
TTGTATATCTATACTATACTATTAAAGCCAAAACATTAAAAGTTTGATTG
GTCGGTCGGTACTTGGGCCTGTGGGCCTCCTTAAACTATAACATGTTTCC
TAGGGACCTCCTATTACCATAATTGATCATTAATAACCGATCACCAATAG
TTAATATAAGTAGATTTCCTCAATTATGAAAAATAAAAATTGCAGATTTT
ACTATATAAAACAGTGATTAATGCAGTACAAATTAGTGATGTAAATAGTG
ATTTTGAAACAGGTTTTTAGTTTGTATTTTGAGAATTGGTTTGTATTTGA
TCACTAGCCTGTGTGTGTGTGTGTGTATGTATGTATGTATGTATGTATGT
ATGCACACACACACACAGTACAAAGTGTTTTAAATTTTGGCAGAAACTTG
AGTAGACGATGGTTTACAAGAGAGTTTTTCTGCATAATTCTCCAAATTTT
GATTGTGCTCCTGACTTTGAAAACTTGTTTTGTTTTACCATGTCTTCCCA
ATATTCAAGTATTTGTATGTTTCTAGATATGTGGACACTGTAAAAGGANA
CTGCTTTCGTTGCTGCTCCCTCCAAAGTTTTATTCTATGATGATTGCTAG
CTTCTGCAGGGTCATTGGTCCTGAGATGCCATCTGCTGAGTTGCTTGCTG
CAGCAGCTAAGTTGACAGAGGCCGAGGCTGAACTACGGTATCTGTACTTG
CCTTCCATATATGATTTTTCATCTTTCCGTTTCGTTATTTTCGTTTTAAA
TATTAGATTAATGGTAATCTGACATGATTTGTTTTAACATGTGTTGCTGC
CTGAATCAGGGATGCAGAGATGGATGGAGGCGATGGAGGATTATTTATTG
GGCCTCCACCTCCAGCTGTGGTGAAGGAAGCTGAATCAGCAAATGAGGCT
GAGCGTTTTGAAGAGGTGCTTTGATTTTTTTTTTTTTATCAATTGTGCAT
CTATAATTATAGTATAGCCACTGCATGCTTGAACACATTCTATTTCTGAT
ATAAAGTTATCAACGTATAGTCAGTACTTGGTAGTCATGTAGTTCTGATA
AGTGGTTTAGATTGCGTGAAACCTAGTTTAGCACCCTTACTTATTGGTAA
AGTCAGCAGTCAAGTTCTTTCTTCCTTTGTAACCATATTTTCTGAAAATA
TATGATATGCTTTTATTCTCTATTGTTTCTTGAGATAATATTGATCTAAA
TGAATCTTCCTTAACACAAGGCCGTTATTAGGCTGTACCAGGGATGGAGG
GAGAGGGGTGTGTGGGGGGACAACTGCTCCCCGAAAGTCTGAAATAGTCA
TGGAATTAGTGTTAAATAATTATATATTTTCTATTAATTCTTTTTAATTC
ATGTACAAGTCCCCTAAAAAAAGTTTATATATGTTCAGATCTTAATTTTT
GATAGAACTGATGATCTTTAGAAAAATTTATGGATCTAAATACAAAACAT
AGCATATCTACACATTCATACTGTTTTATTAATAAATACACACACACACA
CACGCATATGTATTTAACATTTTAATACATTATCAGTATATAATAGCAAG
TCGTAGAATTTTTTTTTGTCCCGTCAATCCTTATTTCTTAACTCCGTCGC
CAGGAAGTACTATTCCTGGATTGCTTACGGATTGATAGGTATTCCTCCAA
GAGTTTTTTTTTTTTTTTTTTAAAAACTTTGCTAGTCTCAGAAAAGAGGC
TAAACTTTGCTTAAAATATGGCTAAAAGACTTGTTTGTCCTATATTTTGT
TCTCCCGGGTCTTCTCTATTTGTTAATATTATATTTAAAGTTAAAACAAG
CATATGCATGGTGTATAACCTATCTGTGGAGATTAAGCTTTTTAAGTGCT
TGTATAACCTATCTCCTAACAATCAATTTGCATACCGCATGTGCTGTATT
ACGATGTGATTTAGAATTTTTTTTTTTATTTTAGTTTGTACTTTTTATTT
TAAGGAGGTTTGTATTGGAGTATGACCCTATATATGCATGTATGTATGTA
TCAGCTTGCCAAAGAATGTATTATACAGTACTGTAGCTTGTATACCTAAT
TGCAGGAGAGTTTCTGTAGGACCAGTGACATTACAATGCTACTGTGTAAT
TGTATACTCTCTCCGTCCCCTTTTACATGTCCATTTTGACTTTTGACAAG
TCAAATTGACTAAATTTTGATTGAACTTTATATGTATTAGATAATTGAGA
AAAATAATAAAAATTATACCATTAGAAAGTATATCTAGTCTATTTTAATA
TGCAACTTTTAGATTTTAGAAATAATGAGTAGATAATTTGTAATGTTTAG
TCAAAAATTGGTCAATTTGACTTCTCGAAAAGCAAAATGGACATGTAAAA
GGGGACGGAGGGAGTATTGATTTTATATTGGAGTTTTATTTGAATTTCCT
GATTTCTTTCTCATGGTTAGGTCACACGGATTATGGGTGCTGATGTTGAT
AGTCCATATGATGTTGTTGGTGTCAACAGAAATATGCCCGCTGAGAATAT
CAAGAAAAGGTCAGTTGCCTGTTAAGTATGAATGAGCCATCTATCAAGTC
AAGATATTAAGTGTCTGCAATACTTTTCTGACCTGGCATCTGAACACAAT
AAACATTGGTAAAATTATGGATGCCATTTACATTTACCTCGCGTTCAAGT
TTTGATAAGGTTTAGACCTTGTCCAAAATATTGGGAGTGCCACTCATCTT
GTGGAGGATATATGCCTATTCTCTGTTTCAAACTTGCATATTAGCTAACA
AGGGAAGGAGACTGGAGATAAACTGATAGTGACTGTGAGTGATTATTGAA
GATTTGATCTTGTTACAGCTGTTACTAACGTATCAGCTTTGTCTGATGAG
CTGTTTTCTTTGATGATGTGGTACATGCTTATATATAGATGTGGGGATTT
AGCCATTCTGGTAGATCTTATATCATGGAATACTTAAGAGATTGTCAAGA
TATATAGACGATATAGTTATTGCTCAAGCCCCCCCTTAAAAATGGTAATG
GGGTTTATTTCTCACCCCCTACCCTTATACAAGTATATCTAACTTGTAAA
GATTGGGAGATTTTTTTTTTAATCTTAGAGCTTCCTATTTAGTATTTATA
TTTTCTCCAATAAGCTCAGGCTTCATTATATTCAAACATTCATGTAATCT
CTTTACATGTCATGGACGGATGATTGTTGAAATCAAGTAATAGTATTCTG
ATTGTAGTCCATGCATATCCTCTATATTAACTGAGAAGTCCTAGTTGCCA
ATATTCTTGGTCAGTTAACCCAAACCTTGCTCTTATAATTTTCTTTATAT
CAGTATATTGCTGTAATTTATTGTCTCTACTCTGTGCCCAACACAGGATA
ATAGAATGATATTGTCAGACATGTAAATCCCATGGGCTTCCATGCCAATA
TAAATGATCCACAAATGTCTTCTAGATTCATTGATGGACTGATGAGTTCT
CTTGACATTCTTTTTAATAAAATTTGAACAAGTTGATGTCATAAATATGA
TTTGGAGTATTTAATTCAGAATTAGTCAAAACAGGGAAAATGTTCATTGT
ATATTGAGTACTTTTGTAGATGTCATCCTATAGATTATACAGATTTCTTT
CATGGCAAATAATTTACACCACAAATTAATTGCCCCGCAAAAGATGTTCA
TCAGTATAATTTTTACCTTGTGATTTTTCTAATTTTATTTGCTTCTCTAG
GTACTGGAAACTGTCCCTTATGGTGCATCCGGATAAATGCCCTCATCCTC
AAGCCAATCAAGCATTTATCATTTTGAACAAGGCCTTTAAAGATTTACAA
GATCCGGATAAGGTGAGCATACTAGTTTGTTAACCTTTAGCATGTCTGGG
AACATGAATTTCATTAAGATTTTGGATTTGATGAAATGACATGTTTAAAT
AAATACAATAATTTGGATTTGGAGTTCATTTGAGATCCTAAACATTTAAA
TACTAGTATAAACTTGGGAATGTGAAATAACAATTGAAATCATGTCAGTT
GAAATCCATCATTTGAAAATGAATTATATGTTCCGAAACACAATCTTATG
GTATTCAAATTTCAAAATGTTTTCCAGTAATGTGATAATGTCTTCTTCTT
CTTCTTCTTCGTTTTTTTTTTTGGCCAAAATCATTGCGAAAATAGATAAT
ATTGGATATAATGAATGTTAGGATTATGGGGTGATTCTTTTAAGTTAGAT
TGATATATTTGTATGTAATCATTACTAGTCATATACCCGCTCCTTTTCTT
ATATCCAAAATATAATTGTATATATACAATCTCATAAATTGATATATATG
TACCGATATAACAGAAAAACAATTGAGTTTCACAATTGTCCAACTTCAAA
TAATTTGTTAATTGTGCTTGATATTCGCATGAAATAAATCCATAACAAAA
TAGTTCAAATAACATTGAGGTAATTTATCATAAACATGTTCTATAGAAGT
TGAAATATGCTATTAAATCAGAAGTGATGATTTAATAATTATTGATACAT
AACATGGAATGGGTTATAGAAGGAAAGAAACTAAAAGGAATATCATTGGA
GTGTGTTATATCGGTAAGATTGGAACAAACAAACCTGTTAATATTAACTA
GTAATATGCCTGTAGTTTGCACACGGTTATACAATAAATCATCAGCTTTA
CAAAATCTAAGCATGAATTTATAAATTATTTTATTTTAATATAATGTTTT
TAGTTATGCTTTTTAAGTTCATGAGCATATATATAAAAATATTGTGACAT
ACATGTGTATGTACTTTTTAACGAATGTGCCTCCTTTAATATATCCTTTA
ATATATTGTAGTAAAATTGATAATAGATTTTGATCAATTATTGTATTTTT
AACCATTAAGATGACGAATTGTATTCCACTATAAAAGCAGCACTGATTTA
TTTCTTTTAGATAGATAACATATTTTCGTATTGACGTGTTAGGAAATTCT
GTAATGTATGAAAGCTAGTATTTTTAATATAGTAATAATATAGTGAAAGT
CATACATGCACGCTAATACAAATCAAATTTGTGTATAAACTTCTTAATTG
ATTTATTAAGTGCTGTATTGGCAAGATTGGAACAACCAAGCCTGTTAATA
ATGATAAGGATGGATGAAATTTATCTTTTTGTAGCATATATCAATCGGTT
CTAATTTGAGTTATGGCAGCTGGTTTATATGAGTGAGTGGAGTGAGTATG
ACAATTTGTCTGCCCTTTTGTAGCGTAAAGCGATGGATGACAAAATTATA
CAGAAAGAGGAACAGGAGAGGTTTAAGGTACATATTTAAACCTCGGATCA
AGTTTACACTGGCTGAATTTAGTTTATTAACTTTGCTTGTACAATGTTTA
TTTGCAGGTAGAATTGAAAGAAATGCGCGAAGCTGCACACTGGAGGCGGT
TGCAAGGTTAATACACTCTCTTTCAGTTATCAATATATTATGCCCTTCCT
TACAGCTTTAGGAGGCAATTATATCAACCTATGCTGAGCATCTCTCTTTT
TCTCATGTTGAATTAATGATGGGAACCTGTTATTTACACGTATATATAAG
TGGTTATATGCATGATTTGCACTTTTATATATTCTAAATTCGACCCTTCA
AATTTGTCTTCTCAATCAAAGCCTAGTTATCTTGTAATAATTTGGTATAT
TCTGTTTACTACTATCCTTATGTCAGAATAATGAGCATAGGCCTAAAAAT
AATAAGCAGTGATATACTTTGAAAGGACATTTACAGAGTAAAGGTATTCT
TCTCGTATAAAAATGGGGTTTAAAATGAAATTGCTCAACTGTATCTGGAA
CAAACACCCCTCACTAAAATTCCTGTTATTGGAAATTACTTGAATATTAA
TTGCTGAACCAGTTCACGTGGTATAGAGCATTTCACTGGCTCTTCTCGTG
ACTGTAAATTTTCCCCCTCTATCTCACACCCACACGTCTTTATTCTATTT
GCAATCAAATACTCATATGGAAGTGCTTGGATTTCTTATTCAAAATATTG
TAGTTAGAACACTAAACCCATCTTAGTATGCAGTAGAACAATTCTTTGCA
CTTTACAGGACGACTTGTTCGCAATTTAATAGTAACTACTGTTCAACAGA
TGATGTTGAACTCGAGCTATAAGCGTTGTACAGAAAATTTTATTTATTTC
TTATTTTTAAAAGGGTGATGTTTTTTTAATTACTATTGACACAAGCACTC
TATATAACTAGAATATAACATGAAGGAAAAAATTTCAACTCATGCAAGTT
TTATTCAATCCGGCTCCTATATATATATATCATAAATACACGATGCAGCA
CTGAGATAATTATGCCAATCATGACGTAGAATACTGAATTTTTGGTGTTT
CCCTCTCATCTCCCCTCTCTCTTCGTCATCCATTTCGATTTGTATGAAAG
TGTATCTATTATTTATTTATTTTGGTAAAACTCGATTGTAAAAATCGAAA
TACTGATAATTGTCAGACACACATGCCTCCACTGAGGCTCGAACCCCCGA
TGCCTGTTGCCAAGAGAAAGTGGGGTTACACTCAGAGGATTTCATTTAAG
CCTGACCCATATGCTCAAACAAACAATATACCAATTATTTAAGTGTTTAT
ATCTCTTGTAAGCATGATTCTCCATTCATTTGGACTGCATGATTTGATAT
CTTTCAAACGAAATACATTTTCTATCTTATATTTGAGGTCAACATGAATT
TAAGACATAAATGTCTTCTGGCAGGTTTATCCATGGAGGGTGATGAAGCT
CTCTTGGCAGATATGGACGTTAAAGTTGAAAAGAAAAGAGACGAATGGAT
GACAACTTTACCTCCTGAGCGAAAAGTATGCATATCTTCTAATCTTGCTG
CTTATCTGTCTTGTTTTCTTAATAGTTCTTATATCGAATTTTCTCAAACA
CAAACCTGACCGACTATAAACAAGTATTCTGGTGAGGTATAGTTGCCAGT
CTTTTTGTTTGCGACCTTGGTAGATGAGAAATCAGTGACCATTTCTACTC
GATGCACACTATCTCCAGGAACAAAATATAGGAGCCAGGAAGCTGTTAAA
TTAAAGAGTAACAAAACCAGTTCTTATGGTTTGTTTTATTACTGAACAAT
ACAGGTCTAGCAATGAGCTTAATGTTGTTGGATCTATTCACCCATATTTA
TGTTAATGAAGAGTAGTAACACTACGGAAATGCATATATCATAATTTTAG
TCAGCTTAGGAGGAAATCTGAAGGGAATCGGAGTGTAGTATTTCTAAAAG
TTTGGAATCTCCCCGAAATACATGTCCGCGTTACGAGCATCTGAGTATCC
ACAAGTGCTGTATGTCTTTAAAGATAGTAAGTTTGTAGTGTTCGACTACG
TACTCCCTTTGTCCAATTAAATTTGACCGCATTTCCTATTTTACTTGTCC
CAAAATGTTTGTCCACATTCAATATTTGAGGTAAATTAATTTCTAATTCT
GAATATGTTAAGTTCTATACATATGGCATTGTTTGAGTGTGAATCTAGAA
AATAAATTGTATCTATAGTAAATTGGTAAAAACATGGATCTTAATTGATG
GAGAAAATGAATTGGATGGTGTAACAGATTTTTGTAAATGAACTTGTAAG
CAGCATTTGCAAAACTTATCTCCATGCTCCAGTAATTGATTATTAATTAG
CAAACTGACATTTTATTTTAACAGCCAGGTGTGACTACTCAGTCTACAAA
ATTTAACAGGAATACTAAAGAAGGCCGTGGTGATACTAGTGCCTGGACAG
ATACCCCTTTAGAGAGAGCTCAGAGAGCAAAAATGAAGTATGTGTATTAT
CTAGTGTTCAAAATCTGTCTAGTGGAATGATTTGATATTAACCCCCTCCT
TGTTTAAATGTCAGTTACTTGGAAGCTTATAATACAGCTACAGCACTTGC
CTCAAATGATCAGCAAAACCAAACCACAAATATGGACGCAGAGTTGGTGG
ACAAGTATAATAAAGCGAAAAGGTCGAAGTCGCTGGTGGAGAAGCATCAA
GAAACAGCTGGTAAGCGTTCGAAGAAAAAAGCGAAGCAAGATTCCTCAAA
GGATGAGTGGCAGGGGCAACATCCATGGAAGCCCTGGGATCGTGAGAAGG
ATTTGACAGCTGGGCGGCAAAATGTTAAACTTGACGCTGAAAACATGTCT
CAAGGTCTATCTTCTAGATTCTCTTCCGGATCATTCCAGAGAAATTTTCT
GTAA