| Relationships |
|---|
| The mRNA, DCAR_018674, is a part of gene, DCAR_018674. |
| The genetic_marker, MK005207 K2651, is adjacent to gene, DCAR_018674. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_018674 | DCAR_018674.mRNA | mRNA | DCARv2_Chr5:32049002..32055497 |
ATGGCGTCAATAAATTTCAACCCATTTGGAGAAAACTGGTTCAAGAAACC
ACCCAACCCATTTCAACCCATTACCCCATTTTTCGATTCCCTAAACCCCT
TTAAACCCCAGCCCAAATCCCCGCTTTTCGCCTCAATTACAGCCTCGAAC
CCCGATACACCCGACCCGGAAGAAAAAAAACCCGGAAAGTACAGGCAAAT
GCTGGACCAATTCTACTGGGAATGCGAAAACCGACCCGATTTTCGACACG
ACCCGGAAATTGATAGGCTAATGAATGAAGACCCACTTGTTGAAAAGAAA
GAGAACCCAACTCAGGAAGAGATTGAGGAGAATGAGAAGTGGTGGAGTGA
GTTTCGAAGCAGCCCGGTTGTGCAGTTCTTGGCACAGGCTGAGAAAATTG
CTGATATGCTTAATGAGATGGAGTTGAAAGAAAATGAGACTCCGTTTCGT
AAGGAGGATAAGAAGTATTGGAGAGATGTTCCTCATGTGATTGGTTTGGA
TGGTAGGCCAATGCCGAGAAAGGCGTTGACACAGAGGGAGGCGGATAATA
AGTTTTGGGATTTTACTAAGCAGTTCTTTTTTGGACTTTGGGGGTTTCAG
CAACGGCCGTATCCGCCTGGGAGGCCTATTGATGCTGCGCAAGCTATTGG
GTATAAGCGGCTTGAGAAACGATATTATGATTGTGAGTTTTTGAGCAATT
TAGCTTTATCTTCGTTGTGATTTTTTTGTTTCGGTTCTAGTTGCTAGTGG
ATGGAGTTGGTTTGTTAAATAATAATGATGAATGTGTATGAAGAATTTAA
GATGATTGCTTGTTTCCTAGATACATTTTGTTTTATGTGGATTATATGGA
GTATGGACCATATGGTAGTAGCTTAAGTTATGTGAATAATTTGCCATTGG
TAGTGTCTTCAATTTTGTTTAGCGAGGTAAGTTCTATTCTCTAGAATAAG
AACATTTCTTTTAGATTGGGGGGGATGAGATGGGGTGAGCATTGAATGAA
ATTCTTACTATAGGGGTAATGATACATTAATTTTCGTTCATTAATTCAGT
CCTATTCCATTCAAACAACTATGTACTAGAGCTCAAATCCCAATTCCACC
AATTTAGGAATGATTGATCATTGCTACCTCCTGCTCATTTCCTTTGCAAA
CATTTATATAGAAATTCGCACTCTTTTTGCTTGCATCATTATCTTTCACC
TATATTTCTGTTTACAATTTTGCATTCCATTCTTAATACATTTCATCATC
ATGCTTAAAGGCCATAATTCTGGTGCTTTTTCTGTTTGCTAAGAAGTTTC
TACCGGCACGAGTGTTATCACTATCCAGTTGTGATTAAGAAGTTTTTTAG
ATTGTTTTTCATACTATTATAAGGCTGTAAATATTACAACACTTCTGGAT
TACTGTTGACATGTGATGTCATGATTGTAGTTATCATGAAGACCGGAGGT
TGGTTCTACAAGGACCGGTTGGGACGTACTAGAGGTCCGATGGAACTTAT
CCAGCTTAAGACTGCCTGGGGTGCTGGAATCATTGATAAACACACCTTTG
TTTGGGGGGAGGATATGGATGAATGGGCACCTATTGGCATGATTTATGGC
ATGGAACGTGCAGTTGCCACATGGGAAGGTTGGTAGATAATTATGTTGAG
CCAAGTTAAAGTTTAAGCCTTTCAGATTATATTTCTTTAAATTTTCTGTT
TATTCTTGTCCTTTCTTTTCTGTTTTTCTATCATTCATTCATTAGGAAGT
CCATTCGGAATGTATTTTTTAGTATCTCTCTCCAAGTATCCGACATCCTG
TACTTATTAGGCTGAATTTTCAGTTAGGCTAGGTGCTGCTGCAACCGCTT
TTCTCCATAAACTCCAAAAAGGTATACCTCCTTGGGCGCCTTTAAAGGGT
TTCGAGAAGAAATCTTATAAGCAGCTCCAAGACGAAGCTTATGAGAGCAA
GAGACGTGATTTGACTGTACTAGAAGCTAATGATGGTGTCTGGCCGGGTG
TAAGAATTCCTAGCCATGCCTTATTTCTTTGGGCCAGTGGTTCTGAGCTC
ACATCTATATTGGAAAATGATCACATGCCAAACAAATACATTCCTAAAGA
TCTGAGGTATCTTTACAGCTGTCTACTTTTCTTTGCTGCATCGTTAAAAG
AGTCTGACATAACTCAAAACTTGAGTAGGACAAGTAACTTGATATAGTAG
CCATTGTTTTTTTATGTTCATGTTCCCTATATTTTAAACAGTCCTGATAC
AATAAAATTTTAAACACATCCTAATTAGTATATGTAATCCTTCCCATTTC
TTCTATTGAAAAGGCTCCAATTGGCTAAAGCTATACCTGGTTTGAGGCCA
TGGGAAGTTCTAAGTGTAGAACAAGCTATGGATCAGATAACTTATAGTGG
TAAGTGGTACCGTGAGCCACTTGGTTCCTTCACTACTGGTCCACCATATA
TTCAACAGTGGAATGAGGAAATGGAGGTAATTCTATTTGCTGTAATGTGC
ATTCAGAACTATTGTGCAGGAAATTATTGCGGACATGCATTAATTGGAAC
ACAAATATTTGTTTTTCCCTTAAAACTTGTCAATGCAGGAACTTTTTGAA
TGCTTTATAAACCTTAACGATGAAGTAGTGAGCGTACTGAAGGAAGCAAT
GCCAGGCTTCAAACCGTTTATGAAGAAACTTGAAGCTGATTATTTTGAAA
GGCTTGAGAGACGCAAAAAGATACGGGAAGAAAAGATTAAGAATAAATCG
TGAGTGTAATGGGAGGAAGTTCTGGGGAGGGGGTGAGTTCTTATGGTCCA
AGGTTTTAAGCATATAGGTCTTTCTTCATCTTAATTCTTATGGGAGCATG
ACCTGGATATACATGTTTTCATATCCATATCCAAATTGAATTTATAATGT
GATGTGATTATCCTTGTGTATCATGTAAAATTGAAGATACACGACTTTCA
TAGTATGTATGGGAATGGGAACTAGTGGTTGCTTTTGAGAGCAACATCTA
TAGAATTGAATTCGTTTTTAATCATATCACTGACAAGGACATCTAAAATT
TAAAAGTGATCTTTATAATTTCTGTGAGGCAAGTCTTTGACGGCATTGTA
TGGTGGAGAGTTTTGAGTTAGCTTAAAAATGTTATTTGTTATTTATAAGT
AAGAACTTATTTGTAAACAAAGAAAATCTTTCAATTTATTTGTACAGATA
AATTTATTTTATACTTTATAAGAAAATAAAATTTATAATATATAATATAA
TATAACAAAGAGAGCGCCAATCAATTGAGGTTGGTAGGAGGTTAATAAAT
ATGTATAAAGAAGTGTGAAATATAAGAAAAAATTGGTAAATTGATGGAAT
TATTATTTTAAAGCAGTATTACAAAAATCGGGAATCGGACCTAATCGGTT
GAGCTACCGATTCAGGGATTAATCGGAGTGAAAATTGGATTTATTTTAAT
ATTAAGATATTATTTAATATTTAGTTATTATTATATTAGCTATTTAGTAA
CTAATATATTTACTATATGCATAAACTCTATAATTATTCATATTTAAAAT
AATATAGTATTTTAATTTTAATAATTTATCATATAAACTTCGATTAAGAA
ATATATATAAGAATTAATCGGATTTCAGAAATCAAATCGGTGGCCGACCT
TATAGGAGATTAATCGGGAATTTTTAGAATACCGTTTTAAAGCGGTTATA
TAGAGTATTTACTAGTTTGTAATATGTTATATGACTTATAATATTTATTG
TTTTTTCAAAAGTATTTTGTCCGAAATTTTATTTCATCATATATTTTAAT
ATTTAATTTTATTTTAAAATGAAATTATAATAGAATAATATGTGTTTTAA
ATAAAATATTTTAATATTTTGAACAACTTTTTAATATTTAATCTATTAGA
TTAGATGGAGAAAATGGGGATCGAATGATATGAGAAATCCCTAATATTTT
CCCCTTCATTTCCAATTATGTAGATTTGCGGTAATTTCATTCAAAAAATA
AAAGATAACCAAAAAAAGAAAAGAGAGACAAAATTTCTTGTGGGCTAAAT
ATTGGTTCAAAAGAAAAGGGCCTAAACGAAGCCCAATAGAACAAAAACCA
AACCCGTACCCAAATAAACACAGTTTAGGGGGCTGCAATATAAAACCAAA
GAGAGGGTCATCTAGGGTTTAGTGTTCGCTGCCATTTACAAACGGATTTG
AGGGAGGCGCAGCTGCAACACCGGCGTTTTAATTAATCCAGCGAAACAGC
TATGGTCAGTTACTTACTAATTCTCATCTCTCTTGTATATCACCTGAATG
TATACGTGTATGTAATCACTCCAATTTGATGAATATGTCTCTGTATTCAC
TCATCTCATTGCTTTTGCAAGCTCGAATGATTGATTTGTTGTGTGATTCT
GAGTAATTGTTTTTATTTGGATCTAAGATTATGTTAATTTAAATTATTAA
CTTTAGCAAATCTCGAATCGGTAGGTTGTATTGAATTAATGCTATGTAAT
ATGTGTGTAGCACGCGGAGCTATTTTCTATCCAATCTTGATAATTTTGGC
TGAAGATTTGCAAAATTATAAAAATGTAGCAATTTTTTTTATAATTTATG
TTGATCAGACAGTGATAGGGGTGGGGTATGGACAATTTGATGCAGCACTT
TTAAATTGTTGATTGTGTTTGCAATGATTGTATAATGTGTTGCTATATTC
TGGAAATGTAGATTAGATAAATGTGTATGTATGAGATGTAGAGCAATATA
AATTTTTAGGACTTGTTTTTAAGGGGTGGCATATGGCTCTTGATATTTTT
GAGCAAGCGTTCTGATCAATGAATTTTTGGTTACTTGTTTTGCGTAGTAG
GATGGCAAATTAAGTTATAATGTATTTTAATCTGAGCTCGGTTCTGGTTT
GATATAGGCGAGAGGTCTTAAGAAGCATTTGAAGAGGCTCAATGCGCCTA
AGCATTGGATGCTTGACAAACTTGGTGGTGCATTTGTAAGTCCCTTTAAA
CTCTGTTCCTATGTTTATTGCTTATTGTGTACTCTAAACAGTTGTTTCTA
ACCATATTGAAATTTGTTTATTCAGGCTCCCAAGCCTTCATCTGGACCCC
ATAAATCCAGAGAATGCTTGCCTTTGATCCTTATATTGCGAAACAAGCTG
AAGTATGCTCTCACTTATCGTGAAGTCCAGTCCATTTTGATGCAGCGTCA
TGTCCTTGTTGATGGGAAGGTCAGGACTGACAAGACCTATCCAGCTGGTT
TCATGGGTATGCATGTTTATTCACCTCATACTAATTGGCATTATTATATT
CCTTTACATATAGTTTACGTGATCACTATAACATGTAGTTTTCTTGGAGT
AGTTTATATCTGACCTATTTAATGTAGTATAGACTTGTGTAGTGCAAGTT
ACCAATGCTAGTTTCCTGTTACAGTTATATTCAGTTCTGTACAAACCTTC
CTAGTGCAACTAAAGGCTTTATAATGATGTTCTGTACAGACTAACTTAAA
TTAAAAAAAAGTTTTACTTTACATGACAACCAACTGTTAGAACTGGGATA
TTTGTATCCAGTTGTAGCGTATGGTGCACTGTTACCTATATATTTTAAGT
AAATTAGTATGTGATGCATAATAAAAAAACGACTTCTGTAGCTATGTTGT
GATGACAATTTTACATTAACTTGCATGTACTTGATCACCAGATGTTGTGT
CAATTCCCAAGACCAACGAGAACTTCCGTCTCCTCTATGACACCAAAGGC
CGATTCCGCTTACACTCTGTTAGAGACGAGGAGTCAAAGGTATGTCTATT
TACCTAATTAATTTGATTCAAGGGATGTGCTAGATGTGAGACTATATCTA
TTTTAACGACAATGGTCTATTGGCTGGAATTAGAGTGTGTATTAACTTTG
TTTGCATACAGTTCTACAATGGTAATATTTCCTTTTAAATACCTTCTAGA
TTTCATATGTTCAAATTCTATATCTCCTTGTGAATGTTAACATTGTTGAT
AAGGATATTTTGTGGACTAACTTGTCCAGTTTGATAAATAGTTGTGTTCA
CCCTTGACAGTTTAAGCTGTGCAAAGTGAGATCAGTCCAGTTTGGTCAGA
AAGGTATTCCATACATCAATACCTATGATGGGCGAACTATCCGCTATCCT
GATCCACTCATCAAAGCTAATGATACCATCAAACTTGACTTGGAGGCAAA
CAAAATTGTTGACTTTATTAAGTTTGATGTTGGCAATGTTGTCATGGTGA
CCGGAGGAAGAAATACAGGACGGGTAGGGATTCTCAAGAACAGGGAGAAG
CATAAGGGGAGCTTTGAGACTGTTCACATTCAGGATGCACTTGGTCATGA
ATTTGCTACTCGTTTAGGGAATGTCTTTACTCTTGGAAAGGGTTCAAAGC
CATGGGTGTCTCTTCCCAAAGGCAAGGGTATCAAATTGACCATCATTGAG
GAGGCCAGGAAGAGGACTGCTGCCCAGGCTGCGACAACTGCATAA