DCAR_022381 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_022381

Identifier:

DCAR_022381.gene

Locus Tag:

DCAR_022381

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 3 relationships.
Relationships
The mRNA, DCAR_022381, is a part of gene, DCAR_022381.
The genetic_marker, MK004861 K2305, is located in gene, DCAR_022381.
The genetic_marker, MK005095 K2539, is located in gene, DCAR_022381.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_022381	DCAR_022381.mRNA	mRNA	DCARv2_Chr6:13162158..13168896

Sequence:

ATGGCGAACCGGCCGCTCCAGACGAACAACATCTCCAACACCGCGAAATC
AATCGAAGAAACCTACCAGAAGAAAACGCAGCTCGAGCACATCCTCCTCC
GGCCCGACACATACATCGGCTCCATCGAGAAACACACCTCCTCTCTCTGG
GTCTACGAAGACAGCGAGATGGTCTCGCGCAGCGTCTCCTACGTCCCCGG
CCTCTACAAGATCTTCGACGAGATTCTCGTCAACGCGGCGGATAATAAGC
AGCGAGACCCCAAAATGGACACTGTCAAGGTCGTTATTGACGTGGACAAC
ACTTTAATTAGTGTGTATAATAACGGCGATGGCGTCCCGGTGGAGATACA
TAAGGAGGAGGGGGTGTATGTGCCGGAATTGATCTTTGGGCACTTGTTGA
CGAGTAGTAATTATGATGATGATCAGAAGAAGACCACCGGGGGGAGGAAT
GGGTATGGGGCGAAGCTGGCCAATATTTTCTCGACTGAGTTTGTTATCGA
GACGGCGGATGGGAAGAGGTTGAAGAAGTATAAGCAGGTATGGTAATGAC
TGTTTTTGTGTGGGTTGATTTGTTTTCTGTTTGATTAGGGCTTTTGTGTG
GGTTGATTTGTTTGTTGTTTAATTAGGGCTTTTGTGTCAATTGATTGGAC
TCCTGTGCTGATTGATTTATTTGTGTTGTTTGATTAGGGTTTAGTATGAA
TGAATGCATAAATGTTGAATGATTAGGGTTATTGTATATATTTATGTAAT
TGTGGAGCAGATAATTACAGTTTTTTGGGTGTAAATTTATGCATGTGTTC
TGTATGCTTTTGTTGGGTGTGTGTGTGTGTGTGTGTTGCTCTGTATGACT
TTGTTAATTAACATTTGATGGCTTCTCTGATGGTGTGCTTTTTAGCTGTT
ATAATGTTGGGTATTTTGTCTCTGAGGGGTTTTTTTGGAATGTAAATTAT
ATGTTTGTCTGTCTGATTTTATGCATTGGGGATCCTGGTAGCTTGGTTAT
TCTATGTGCTTGTATTAAGCATTTTTATCTGTGGACATATTGTTGGGATG
CTATTGTAGAGAAAATGATACTATGAGTACAGAGCTAGTAATTTACTTTT
GTATGTAGAGTATGAGTGAGGTAGTAGTGCCTACATCTTTGAATCATGTC
AATATCTAGATCATTAGGTTCGTGAATATAGCAAATTTTACTATATGTTG
AAATTTGATATTTTAATGTTATGATGGTTTTCGGGATTCAGTATAAATTC
TCATTTCGGATTGGTGAATTGAAATGTACAGGTGTTCTCAAATAATATGG
GAAACAAAACTGAACCCCATATAACAAAGTGCAAGGAATCTGATAATTGG
ACAAAAGTTTCATTTAAGCCTGATTTAGCGAAATTTAATATGACTCATTT
GGAAGATGATGTTGTAGCCCTGATGAAGAAACGAGTGGTGGATATAGCAG
GGTGTCTTGGGAGGTCCGTGAAGGTGGAATTAAATGGTGAGCGAGTTCCA
ATAAAATCTTTCACTGATTATATCGATCTGTATCTGAAATCGGCTAACAA
GGACAAAGAAGATCCCCTTCCCAGGTTAGTTTCTTGGAAATTCCTTAGTT
ATTGAAGTTTTTGCATATTAGAATGCTGATTTGACTGAATAATATGCAGA
TTTGTTGAAAGAGTAAACAGCAGGTGGGAGATTTGTGTCAGTCGGAGTGA
AGGACAATTTCAACAGGTAATATACACAACTCAATGAGTAGCAGCGTTCA
TACAAGCTCCTAGTTGATACATTGTTAATTATCTATCTGTTGTTATACCA
GGTCAGTTTTGTAAATGGGATTGCAACCATCAAGGGTGGCACTCATGTTG
ATTATGTTACCAACCAGTTAACAAGTCACATCATAGCCTCTATAAGTAAG
AAGAACAAGAAAGTGAACCTCAAGCCTCATGCTGTGAAGAACCACCTATG
GGTTTTTGTTAATGCACTAATTGATAATCCTGCTTTTGATTCCCAAACTA
AGGAGACACTAACAATACGGCAAAACAGTTTTGGTTCGGAGTGTAAGCTT
TCAGAAGATTTCTTAAAAAAAGGTTTGTCTGCAGAAGCATACTCTGTAAC
TTATAATGAATAGGAACTGTTTGACACTATGGATTAAGTTCTTATCAATG
ATAACATTTCCATTGTAGTGGTGAACAAATCTGGAATAGTAGACAGTCTG
CTCGCATGGGCAGAGTTCAAGGAGAAAAAAGACTTAAAGAAAACTGATGG
TAAAAAGGGTCGAGTGACTGGCATTCCTAAACTAGAAGATGCTAACAAAG
CTGGTGGGAGCCAATCTCAAAAATGCACCCTAATTTTGACAGAAGGAGAT
TCAGCTAAGAGTCTTGCAGTAAGTTCTATCCTTCTTACTTGGATTTGTGA
CTCTTGGTACTTTATATGTATGAACTCTGATAATTTGGTTTACATATAGA
TGGCTGGTCTTTCTGTTGTGGGACGAGATCTTTATGGTGTATTTCCCTTA
AGAGGTAAGCTGCTTAATGTGAGAGAAGCAAGCCATAAGCAGATAATGGA
GAATGCTGAAATTCAGAATATCAAGAAGATACTTGGACTCCAACATGGTA
AAGCATATGAAGATGTCAAGTCATTGAGATACGGTCATCTGATGATAATG
ACCGATCAGGTACATACCTTTTGAATATATTACTCGTCACTGAAAATTTT
ACTCATTGACTGTTTGATAATGATAAATAAGAATATGACAATGCTGAAGT
TTCTTTAGTATCCATTTGCTAAATTCAAACTTGTGTGCGGGCAGGATCAT
GATGGTTCCCACATCAAAGGGCTCTTGATCAATTTCATTCATTCTTTCTG
GCCATCACTGCTAAAAGTTCCACATTTCATGATAGAGTTTATCACTCCAA
TTGTGAAGGTTTGCAGCTTACACTGAAACTGGATAAATCAACCATATTTA
CGCATATATTGCCAGATTTGTGAACGATTTTTTCCATTTTGCTTAATTAT
GAATATGTTTTCTATGATTCCCCCTGTACAGGCTACCAAAAATGAAGGTG
CCAAAAATGAAATAGTTTTGTCCTTCTATACGATTCCTGAGTACGAAAAT
TGGAAGGAAAGTTTGGGGGATGCTCTACGTGGCTGGACAATAAAATACTA
CAAGGTTTGTTTCTGTGAATTACAATGCATGTATGGCAGTGCCAGTGATA
ATATAAGGCTTTGTTGACAATGTATTGCTATTGTACCAGGGGCTGGGAAC
TAGCGATGCAACTGAAGCAAAAGCATATTTTAGGGACCTTGGAACACACA
AGAAGGATTTCATATGGGCTGGTGATGATGATGGTGATGCAATAGAGCTT
GCCTTTAGTAAGAAAAAGATTGAGGCAAGAAAGAACTGGTTGAGGCAGTT
TGAGGTTAGGTTTACAATTTTGTAAGTGATTTGTGATTTTTATGGTGATC
ATTATATTAACTCCTTCATATTTTCCTGTTCCAGCCTGGTACTTTCTTAG
ATCAAAAAGAGAAGCTGATCAAGTATAGTGACTTTGTCAACAAGGAGCTT
ATACTGTTTTCTATGGCGGATCTTCAAAGGTCTATCCCATCAATGGTTGA
TGGCCTGAAACCAGGACAAAGGAAAATTCTTTTTTGTTCTTTCAAGAGGA
ATTTTACCAAGCAAGCCAAAGTTGCACAATTCTCAGGTTATGTCTCGGAG
CACTCAGCTTACCATCATGGTGAGCAGAGTCTTGCAAGTACAATAGTTGG
TATGGCTCAGGATTTTGTAGGTAGCAATAACATAAACCTTCTTCTACCGA
GAGGGCAGTTTGGTACACGAAACCAGGCAAGACAATCTGAAGTTTTCACT
TATTGCAAATTAACATTAAATACTTGCAGCATGTATATTAACTTCCTAAC
TTACAGGGTGGCAAAGACCATGCAAGTGCTAGATACATTTATACTGAGCT
TGCTCCAATCACTAGGTACATGTTTCCCAAGGATGATGACATTCTCCTTG
ATTACTTGTATGAAGATGGACAAAAGATAGAACCTTACTGGTAATTAGTT
CTTACACATTTTAGCCAATGAAAATAAAATACTCCCTATTTTTACATCAA
ACTTCCATTGTATAATTTGTGTTTCTTTCTGTGATCAAGGTATGTTCCTG
TTGTACCCATGGTTCTAGTAAATGGAAGTGAAGGAATAGGAACAGGTTGG
AGCACATATGTGCCAAACTACAATCCAAGAGATATTGCTGCTAATATAAG
ACGGTTGCTAATGGGGGAACCAATGCAGCCAATGGATCCATGGTACAAAG
GATTTAGGGGAACCATTGAGAAATCTGCAACAAAGGAAGCTGGAGCAGGC
TACACTGTCAGTGGGCTTATAGAAGAAATAGATGATACTAATGTTAGAAT
CACAGAGTTGCCTATTCGGAGATGGACCCAAGATTACAAGGAGTTTCTGG
AATCAAGCATGATCGGAAATGAAAAAATAAAAGCATTTATCAAGGTATTA
TCAATGTCAGCTTGTTTAGCTTTATTACTAAATGTGTTGGAAGCCTCATA
CTTGCAAACCATCTTTTACAGAACTACAGCAACCAGAGCGATGACTGCAC
TGTACATTTCGATGTAGAGATGTCTGAGGAAAACATGCTCATTGCTAAGC
AAGAGGGGTTGCTAAAGAAATTTAAGCTAACAGCTAATATAAGCACAAGT
AACATGCACCTCTTTGACCCAAAGGGTGTAATCAAGAAATATGACTCCCC
TGAACAAAGTATGCAATTTATGTTGTTTTAGCATATTTAGTCAGTACCAC
TTCAAAAATATTTGGACTAACTCAAACAAATTTATTGTTGTGGCAGTTCT
TGAAGATTTCTACCATGTGCGGTTTGACCTTTACAAGAAACGGCAGGCAA
GTTAAATAAATTCAGAGTTACAATTACCCAGTAATTTGGAACATGTTTAA
TTCATTTATTGCACATAATGGCCGCTAAATTTGGGGTTATTTGTCATGCA
GAAAGTTCTGCTGGAAAATCTAGAATTGGACTTGCTTAAACTTGATAACA
GGGTCAGGTTTATTCTAGGGGTCGTGGAAGGAAAAATCAAGGTGAGCAAC
AGGAAGAAAGCAGATCTATTCCTTGAGTTGAAGGAAAAAGGTTTTACACC
TTTTCCGAAGAAGACCAAGGTAGAAGAACTTGTTGCTGGGGCCACTGATG
CTGTAGAAACTGAAGATAATTCTCAAGGAAGTGGGGGTGCAGGAAGCGCC
AAACTTGGTGACTATGACTACTTGCTGAAAATGGAAATTGGAACCTTGAC
CGCTGAGAGAGTTCAGAAGCTACTTAAAGAGAAAGATCAGCTCATTGGAG
AGGTTGATGAACTGAGAAAGGCAACTCCAACGTCTCTTTGGCTAAAAGAT
TTGGACGCTCTTGATAGAGAACTTGATGTATGATTAACAATACCTGTATT
TCTTGTTTGTTTCCTCTGTTTCCCTTCTCTTGCTTGATATATTGGCATTT
ATAGGCACAAGATCAGAGAGATGCTGATGGTGAAGCATCCCGAGTATCCA
CTTATGAGAATAGGATGAAACCAGATGGTTCTGCAAAAAAGGCACCAAAG
AATCCTCGGAAGAGTACTAAAAATGTCAAAAACGCAGAAACAGTTGTTAA
ACTGACAGGAACGGCAACTGATTCAGCAATGGAAACAGGTATATATGCAC
TAGCGCGATTGAATATTTACTGGAAATTCATTTAGTCAATTCTTGTTTAC
TGATTGTGAATTTCTTCAGAAGACAATGTTCAAGAAGTAGTGAATCCCAA
AGGCAAAGCTGGACCAAAAAAAGCTCCTGCTAAAAAGGTTGGATATCAGA
GCTAATGTCACAATTTGCAGTTTTCTTTGTACGAATGAATTTTATTGTTA
CTAATATACTTCCATTTGGCAGGGAAAATCAACTTCCATCTTACGAGATG
AGGATGATGATGATGAAATACTTGATCTTAAAGACAGACTAGCTGCCTAC
AACATCAATTCTTCACCTGATCATTCGGAAGGTCAGGATGATGCTCTACT
TTACTTATTGAGTTTTGATTGTGCTTATTTTATTGAGTCGTGGTTGTGTA
TTTGTTTTTACACAGTAATGGAAGTTGATGTCCCTCAAAAACAAGCCACG
AAGAAACCACCAAGTGGGAAGAGTGTGGCTGGGAAGAAGGCTTCATTGGA
TGTTTCTGGAGACGAAGAACAAGAGTTTGAGATTGAGGATATTAGTGACG
ACAATGACTCTGAAGTTGAGGTTGTGAAAGGAAAGAAAGGAGGAAGAAAA
CCAGCAGCAGGAAATTCCAAGGCAGCTGCTAAACCACCTGCTGCACCAGC
AGCAACTAAAAAGAGAGGGCCCGCTGCAACTACTAAGAAGTCTCAATTGG
TTAACCAGAAGCTGATCACAGATGTTCTAAAGCCTGCCGAAAACTCTGGG
GTGTCACCTGAGAAGAAAGTAAGGAAAATGAGGGCATCTCCATTTAACAA
AAAGAGTAGTTCTGTCTTGGGAAGAGTTGCTGCCAAAGATGATAGTGTTT
CACTAGGTTCTAGCTTAGACACAAGTGGCGGTGGAGGCTCTAGTGAAACG
ATGACTCGAGTGGCTAGACCACAGAGGGTAAACCGCACCAAGACAACTTA
TGTACTGAGTGACAGTGATGACAATGATGAAGAGGATCCTACTGAAGAAG
AGCCTACTGACGATGATGACTTTGAAGATGAAGATTAA

Sequence Length:

6738

Sequence Checksum:

11df39393570fb536b70610795a8ce69

View location in JBrowse:

DCARv2_Chr6:13162159..13168896-

Cds:

>DCAR_022381cds ATGGCGAACCGGCCGCTCCAGACGAACAACATCTCCAACACCGCGAAATC AATCGAAGAAACCTACCAGAAGAAAACGCAGCTCGAGCACATCCTCCTCC GGCCCGACACATACATCGGCTCCATCGAGAAACACACCTCCTCTCTCTGG GTCTACGAAGACAGCGAGATGGTCTCGCGCAGCGTCTCCTACGTCCCCGG CCTCTACAAGATCTTCGACGAGATTCTCGTCAACGCGGCGGATAATAAGC AGCGAGACCCCAAAATGGACACTGTCAAGGTCGTTATTGACGTGGACAAC ACTTTAATTAGTGTGTATAATAACGGCGATGGCGTCCCGGTGGAGATACA TAAGGAGGAGGGGGTGTATGTGCCGGAATTGATCTTTGGGCACTTGTTGA CGAGTAGTAATTATGATGATGATCAGAAGAAGACCACCGGGGGGAGGAAT GGGTATGGGGCGAAGCTGGCCAATATTTTCTCGACTGAGTTTGTTATCGA GACGGCGGATGGGAAGAGGTTGAAGAAGTATAAGCAGGTGTTCTCAAATA ATATGGGAAACAAAACTGAACCCCATATAACAAAGTGCAAGGAATCTGAT AATTGGACAAAAGTTTCATTTAAGCCTGATTTAGCGAAATTTAATATGAC TCATTTGGAAGATGATGTTGTAGCCCTGATGAAGAAACGAGTGGTGGATA TAGCAGGGTGTCTTGGGAGGTCCGTGAAGGTGGAATTAAATGGTGAGCGA GTTCCAATAAAATCTTTCACTGATTATATCGATCTGTATCTGAAATCGGC TAACAAGGACAAAGAAGATCCCCTTCCCAGATTTGTTGAAAGAGTAAACA GCAGGTGGGAGATTTGTGTCAGTCGGAGTGAAGGACAATTTCAACAGGTC AGTTTTGTAAATGGGATTGCAACCATCAAGGGTGGCACTCATGTTGATTA TGTTACCAACCAGTTAACAAGTCACATCATAGCCTCTATAAGTAAGAAGA ACAAGAAAGTGAACCTCAAGCCTCATGCTGTGAAGAACCACCTATGGGTT TTTGTTAATGCACTAATTGATAATCCTGCTTTTGATTCCCAAACTAAGGA GACACTAACAATACGGCAAAACAGTTTTGGTTCGGAGTGTAAGCTTTCAG AAGATTTCTTAAAAAAAGTGGTGAACAAATCTGGAATAGTAGACAGTCTG CTCGCATGGGCAGAGTTCAAGGAGAAAAAAGACTTAAAGAAAACTGATGG TAAAAAGGGTCGAGTGACTGGCATTCCTAAACTAGAAGATGCTAACAAAG CTGGTGGGAGCCAATCTCAAAAATGCACCCTAATTTTGACAGAAGGAGAT TCAGCTAAGAGTCTTGCAATGGCTGGTCTTTCTGTTGTGGGACGAGATCT TTATGGTGTATTTCCCTTAAGAGGTAAGCTGCTTAATGTGAGAGAAGCAA GCCATAAGCAGATAATGGAGAATGCTGAAATTCAGAATATCAAGAAGATA CTTGGACTCCAACATGGTAAAGCATATGAAGATGTCAAGTCATTGAGATA CGGTCATCTGATGATAATGACCGATCAGGATCATGATGGTTCCCACATCA AAGGGCTCTTGATCAATTTCATTCATTCTTTCTGGCCATCACTGCTAAAA GTTCCACATTTCATGATAGAGTTTATCACTCCAATTGTGAAGGCTACCAA AAATGAAGGTGCCAAAAATGAAATAGTTTTGTCCTTCTATACGATTCCTG AGTACGAAAATTGGAAGGAAAGTTTGGGGGATGCTCTACGTGGCTGGACA ATAAAATACTACAAGGGGCTGGGAACTAGCGATGCAACTGAAGCAAAAGC ATATTTTAGGGACCTTGGAACACACAAGAAGGATTTCATATGGGCTGGTG ATGATGATGGTGATGCAATAGAGCTTGCCTTTAGTAAGAAAAAGATTGAG GCAAGAAAGAACTGGTTGAGGCAGTTTGAGCCTGGTACTTTCTTAGATCA AAAAGAGAAGCTGATCAAGTATAGTGACTTTGTCAACAAGGAGCTTATAC TGTTTTCTATGGCGGATCTTCAAAGGTCTATCCCATCAATGGTTGATGGC CTGAAACCAGGACAAAGGAAAATTCTTTTTTGTTCTTTCAAGAGGAATTT TACCAAGCAAGCCAAAGTTGCACAATTCTCAGGTTATGTCTCGGAGCACT CAGCTTACCATCATGGTGAGCAGAGTCTTGCAAGTACAATAGTTGGTATG GCTCAGGATTTTGGTGGCAAAGACCATGCAAGTGCTAGATACATTTATAC TGAGCTTGCTCCAATCACTAGGTACATGTTTCCCAAGGATGATGACATTC TCCTTGATTACTTGTATGAAGATGGACAAAAGATAGAACCTTACTGGTAT GTTCCTGTTGTACCCATGGTTCTAGTAAATGGAAGTGAAGGAATAGGAAC AGGTTGGAGCACATATGTGCCAAACTACAATCCAAGAGATATTGCTGCTA ATATAAGACGGTTGCTAATGGGGGAACCAATGCAGCCAATGGATCCATGG TACAAAGGATTTAGGGGAACCATTGAGAAATCTGCAACAAAGGAAGCTGG AGCAGGCTACACTGTCAGTGGGCTTATAGAAGAAATAGATGATACTAATG TTAGAATCACAGAGTTGCCTATTCGGAGATGGACCCAAGATTACAAGGAG TTTCTGGAATCAAGCATGATCGGAAATGAAAAAATAAAAGCATTTATCAA GAACTACAGCAACCAGAGCGATGACTGCACTGTACATTTCGATGTAGAGA TGTCTGAGGAAAACATGCTCATTGCTAAGCAAGAGGGGTTGCTAAAGAAA TTTAAGCTAACAGCTAATATAAGCACAAGTAACATGCACCTCTTTGACCC AAAGGGTGTAATCAAGAAATATGACTCCCCTGAACAAATTCTTGAAGATT TCTACCATGTGCGGTTTGACCTTTACAAGAAACGGCAGGCAAGGGTCGTG GAAGGAAAAATCAAGGTGAGCAACAGGAAGAAAGCAGATCTATTCCTTGA GTTGAAGGAAAAAGGTTTTACACCTTTTCCGAAGAAGACCAAGGTAGAAG AACTTGTTGCTGGGGCCACTGATGCTGTAGAAACTGAAGATAATTCTCAA GGAAGTGGGGGTGCAGGAAGCGCCAAACTTGGTGACTATGACTACTTGCT GAAAATGGAAATTGGAACCTTGACCGCTGAGAGAGTTCAGAAGCTACTTA AAGAGAAAGATCAGCTCATTGGAGAGGTTGATGAACTGAGAAAGGCAACT CCAACGTCTCTTTGGCTAAAAGATTTGGACGCTCTTGATAGAGAACTTGA TGCACAAGATCAGAGAGATGCTGATGGTGAAGCATCCCGAGTATCCACTT ATGAGAATAGGATGAAACCAGATGGTTCTGCAAAAAAGGCACCAAAGAAT CCTCGGAAGAGTACTAAAAATGTCAAAAACGCAGAAACAGTTGTTAAACT GACAGGAACGGCAACTGATTCAGCAATGGAAACAGAAGACAATGTTCAAG AAGTAGTGAATCCCAAAGGCAAAGCTGGACCAAAAAAAGCTCCTGCTAAA AAGGGAAAATCAACTTCCATCTTACGAGATGAGGATGATGATGATGAAAT ACTTGATCTTAAAGACAGACTAGCTGCCTACAACATCAATTCTTCACCTG ATCATTCGGAAGTAATGGAAGTTGATGTCCCTCAAAAACAAGCCACGAAG AAACCACCAAGTGGGAAGAGTGTGGCTGGGAAGAAGGCTTCATTGGATGT TTCTGGAGACGAAGAACAAGAGTTTGAGATTGAGGATATTAGTGACGACA ATGACTCTGAAGTTGAGGTTGTGAAAGGAAAGAAAGGAGGAAGAAAACCA GCAGCAGGAAATTCCAAGGCAGCTGCTAAACCACCTGCTGCACCAGCAGC AACTAAAAAGAGAGGGCCCGCTGCAACTACTAAGAAGTCTCAATTGGTTA ACCAGAAGCTGATCACAGATGTTCTAAAGCCTGCCGAAAACTCTGGGGTG TCACCTGAGAAGAAAGTAAGGAAAATGAGGGCATCTCCATTTAACAAAAA GAGTAGTTCTGTCTTGGGAAGAGTTGCTGCCAAAGATGATAGTGTTTCAC TAGGTTCTAGCTTAGACACAAGTGGCGGTGGAGGCTCTAGTGAAACGATG ACTCGAGTGGCTAGACCACAGAGGGTAAACCGCACCAAGACAACTTATGT ACTGAGTGACAGTGATGACAATGATGAAGAGGATCCTACTGAAGAAGAGC CTACTGACGATGATGACTTTGAAGATGAAGATTAA

Pep:

>DCAR_022381pep MANRPLQTNNISNTAKSIEETYQKKTQLEHILLRPDTYIGSIEKHTSSLW VYEDSEMVSRSVSYVPGLYKIFDEILVNAADNKQRDPKMDTVKVVIDVDN TLISVYNNGDGVPVEIHKEEGVYVPELIFGHLLTSSNYDDDQKKTTGGRN GYGAKLANIFSTEFVIETADGKRLKKYKQVFSNNMGNKTEPHITKCKESD NWTKVSFKPDLAKFNMTHLEDDVVALMKKRVVDIAGCLGRSVKVELNGER VPIKSFTDYIDLYLKSANKDKEDPLPRFVERVNSRWEICVSRSEGQFQQV SFVNGIATIKGGTHVDYVTNQLTSHIIASISKKNKKVNLKPHAVKNHLWV FVNALIDNPAFDSQTKETLTIRQNSFGSECKLSEDFLKKVVNKSGIVDSL LAWAEFKEKKDLKKTDGKKGRVTGIPKLEDANKAGGSQSQKCTLILTEGD SAKSLAMAGLSVVGRDLYGVFPLRGKLLNVREASHKQIMENAEIQNIKKI LGLQHGKAYEDVKSLRYGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLK VPHFMIEFITPIVKATKNEGAKNEIVLSFYTIPEYENWKESLGDALRGWT IKYYKGLGTSDATEAKAYFRDLGTHKKDFIWAGDDDGDAIELAFSKKKIE ARKNWLRQFEPGTFLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG LKPGQRKILFCSFKRNFTKQAKVAQFSGYVSEHSAYHHGEQSLASTIVGM AQDFGGKDHASARYIYTELAPITRYMFPKDDDILLDYLYEDGQKIEPYWY VPVVPMVLVNGSEGIGTGWSTYVPNYNPRDIAANIRRLLMGEPMQPMDPW YKGFRGTIEKSATKEAGAGYTVSGLIEEIDDTNVRITELPIRRWTQDYKE FLESSMIGNEKIKAFIKNYSNQSDDCTVHFDVEMSEENMLIAKQEGLLKK FKLTANISTSNMHLFDPKGVIKKYDSPEQILEDFYHVRFDLYKKRQARVV EGKIKVSNRKKADLFLELKEKGFTPFPKKTKVEELVAGATDAVETEDNSQ GSGGAGSAKLGDYDYLLKMEIGTLTAERVQKLLKEKDQLIGEVDELRKAT PTSLWLKDLDALDRELDAQDQRDADGEASRVSTYENRMKPDGSAKKAPKN PRKSTKNVKNAETVVKLTGTATDSAMETEDNVQEVVNPKGKAGPKKAPAK KGKSTSILRDEDDDDEILDLKDRLAAYNINSSPDHSEVMEVDVPQKQATK KPPSGKSVAGKKASLDVSGDEEQEFEIEDISDDNDSEVEVVKGKKGGRKP AAGNSKAAAKPPAAPAATKKRGPAATTKKSQLVNQKLITDVLKPAENSGV SPEKKVRKMRASPFNKKSSSVLGRVAAKDDSVSLGSSLDTSGGGGSSETM TRVARPQRVNRTKTTYVLSDSDDNDEEDPTEEEPTDDDDFEDED*