| Relationships |
|---|
| The mRNA, DCAR_024620, is a part of gene, DCAR_024620. |
| The genetic_marker, MK004295 K1739, is located in gene, DCAR_024620. |
| The genetic_marker, MK007830 TP94137, is located in gene, DCAR_024620. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_024620 | DCAR_024620.mRNA | mRNA | DCARv2_Chr7:17103955..17110888 |
ATGTGTTCACGAGGCGACGATGAAGATGACGATGATGAGATCCAAGAGAA
CAAGAAAAATTTGAATCCTCAAATTGATAATGCGGAGACTGAATTTATGG
ATACTCAACCTTCTCCGTTACTGCTTCAAGGTAAATTATTTAGTTAATTT
ATCGAGATTAAATTATCTATTATGCTGGGATAGATAAATTGTATGGGAAA
ATAGATTTTTTTGCCACCCAACTTATTATTTGTTTAGAAACATACCACTG
AACAATAATTTTTTTCTTTTTTGCCACTAATGTTAGGTTCGGATTTTTTT
TTGTCACTAACATTAGGTTCGGATTTGATTATTAACACTATGTTATTCTT
CTTAGAGTTAATTGCATTTGGCACCCCTTTGATTTTAGCATGTTGCACAT
TGCACCTAATAGTTTTGGAATAAACACTATGCAGCCCATTACTTTTAACC
CGTAGACACTTTGCACCCTTTCCGTTATATTCTGCCGTTACCGTTAACTT
TTGAATGGGCATTTTGGGAAATTTTTTTATATTTAATTAAAATCAAACCT
TTATTTAAATTTTCCGACTATCCAAACGATATTTTTCGGAAATCTATTTT
TGGGTAGTCTGCAATATAATAGTGATCTGTGTCTATATCTTTATATGTGG
TACTCCATATGTGTCCTAGGTAGTTTATCTTAGAGGACGAGATTACAAAA
TTTTTTACTCAGAAAGGAAATCTCAAAATTAACTTATGGACTTAAATTTA
TAAAAAGTGGGAACCTTAAAATACGTGATAAGTAGTGGTAAAGAACTATA
AAGTCAAGTGATACTATATATTTGTGGAGTCTTAAAATAATTCATAAAAC
TAAACTTTTTAGGTTCTTAAAATGCCTGTTAAACTCTCGTACTTCAAGGT
AAACTAGCTAAGGGACATATAGATAACTGTATATAATGTTATAGACACAT
ATCATATTACCATAATATTGCAGACTACCACTATGTTTTTATAATACTAA
AAAAATAACATATTGTTAATAATCAAATCAAAACATAACGTTAGTGATAA
AAAAGAAATCCAACTTTTTTTAAGGAAAAAAAACCAACATTAAATTTTAT
TCAGAAAAAATAAAATAAAATAATTTTTGAAATTTTTTGGAGTCTCAAAA
TGCGTGTCCAACTCTCGTCGTCCAAGGTAAACTACCTAGGACACTACATA
AAGATATAGACACATATCATATCGCCATTATATTGCAGACTAACACTATA
TTTTAATAATAATTAAAAAATAACATAGTGTTAATAATCAAATCCGAACC
TAACGTTAGTGACAAAAAAAAGTCTGAACCTAACATTAGTGACAAAAAAG
AAAAAAATTATAGTTCAGTGGTAGGTTTCTAAACAAATAATAAGTTGGGT
GGGAAAAAAATCTATTTTCCCTAAATTGTATTGATTTGTTAACATTCTAC
TTTTGGTAAGATGAAGAATTGGTGCACACAGTTATGTTAAACGAATATTT
TGTTATCAAATTTTGCATAATACCACCAGCAATTGAAAAAAAAAGTTATT
ATGTTCGTCGTTCGGTTCTGACTAGTATATGTTGAAATATGATGCTGAGG
AAGTATGAAATCGTTATTAGACCAAGTAGAAGAGTGATGATACGTTATGG
TTTTGTGTCAGGATATGAATCGTATGATGATGCGGTTACTTTAGAAGATG
CTGAGACTCAAGGGGTATGCCTTGCTGGTGAGACTCAAGCATTGTGTTAT
GATGGTGAAACTGAAGCAGTTGATGATGTGGATTGTGCAGAAGATTTGTG
CACCCAATTGTTTGATGACCATGATACAGAAGTCGTGGTTGATGTTGACG
ATGAAGGAGCCAAGAGAAACGGAGTTTTAGATGACACTGAGGCGTTGTCT
GATGTTGACTGTGTGGAAAGAGTTGACAATCATACAGTGGTTCAAGACAT
AATGCTGCAAACCTGTCTGCAGAAACAGGATGATGGTGATTTTAAAGAGG
TTGAGAACACTGAAGAGTGTGATACAGGTCCAAAGACTTGTGAATTTAAT
TTTTTTTAAAAAAAATTATATATTAAGCGCTCGAGTTTAATTTTGACATC
AGTATTATGTATAAACCATAAAGTATAACTAGAAAACATGCATGTTTGAT
GTTGGCGAGAAAACTAAGTATCATTCCTGTGATAACAAAATGAGCAATAC
TATCTATTGGAATGAAAGGCCAATAGTCTTTTATTATCGTGGATGTGTAT
TGTTCATTATTTTACCTTTAACAATATCATGTACAAATTTGTAAAAATAT
TAACATCTTGGTTTTATGGCAGCAAATAACCAATTAAGTGGATGATTTTG
GAGGATAGGTAAGTGGATGATTTTGGAGGATAGGTATAATCTTCACAAGT
GTTTCACAATAGATGTGGTTTTTTTGTGTGCCAACATACGCTTTATTTCT
TCTATTAGTTTCAAGTTTCAAGAATTTCCTGTACATACTTCCATCCCACA
AGCTGCCTTCCTTACCTTTGTTAGCACGTCGTGTCTTTTTAAAAAAAATC
TAAAGCCAAACTCACATTTATGTTTAGTTTTTGCCTGAATATGGTATGTA
TAAGAAGCTATAAACTCAGTCTAATAGTGAAATTTTCCGTCATCATGCAG
CTCCAGTCCTCGAAGGATTTACATCACTACGTGTAGCATCTATTAGGTCT
TCTGGTTTGGCTGCTCGCAACATGTCTTTGAGACGAACTAATAACACTTC
ATATTACAGTATGAGTAGCAGTCAACATGAAGTAGAGAGTAGCGGGCCAA
GCGTTATTGACAGTTCATTAAAAGTTTTGGGGCTAATTGATCAAAGGCAT
AGTCTGGAGGAGCTTGATGATAAAATAAAAGGATCTAGTAGTGAAAACAA
GTGCAGGGTTGGCAAGTCAGCAGTTAGGAAATTACTATATGAAGATATGT
CTCATGAGGAAGGGCTTGATATAAATACTAATAACACTGATAAAGAAGAA
AACTTCCCCCCAGTCTGCAATTCTGATATGGAGTTGGCAGGCCTGAGCTA
TGTAGATTCTCAAGAGCCCGGAGAGGCTTCACAAACTATTGCTCTTGACT
TTGTGGACCAATTTCTGAAAGTTAATGTTACAGAATTAGATCAAGAAGCT
GAAATTATACGGTCAACTGGGGGGAAATTAAAGCCTCTCCCAGGTGCTAG
AGGAACCCAATTTCTGGCTAAACGTGCTAATCGCATTGGTTCAGCATCTG
GTGGGACATTTGACTGGGATGATTATCAAGAGGATGGAGGGGGTCAGTTT
TTAAGAAAAGAGAAAGGAGCATTTTCAAATGATGGACGCAGAGCTCATCA
ATCCCTTCCCAAATCTCAGGATCTAAAGCATCTGAATCCAAATAATTGTT
GGTCAGTCAAAGAAAACGGTGAGAAGGGAGAGCAACATGACCTATGTAAC
AGTGTAGCTGGCTTATTTGACTCCGATTCAAAGTTACTTTTGCACAAAAC
AAAAAAGATTGATGAATCAATGAAGGTGGCAGGAAAGTTCATTGGACAAA
ATCTAATTAAAAAGTTGAATGATGCCAGAGATGGAATGGTTGAAACAGGT
ACTCAAAAAGATAATCCAGACATGCTGGACATAGGAGTTGATACTCAACT
GGCTGCTGACGCAATGGAAGCCTTGTGCAATGGGGTAGGTTTGAATGACG
AAGAAAACAATGTTACTAATCAGGGTGCTGAGATCCATTTTCTTAGTAAT
GAAAAAGGAAATTTTTTAAGAACATTAGTGTCAGATAGAGATTTTGTTCA
AAAAAGAGCATCACGCTCTCCAGGATCTGCACTCACTACAAGGCAAACGA
ATCAAACTAAAAGGTATGGTACTGGATTGACTAAAGGGTTCTCCATTTTA
TCAGAACAGCAGTCCAGAAAAGCTAGGAAGCAGTGTGACGAGACTGAAGA
TATGTGCAACTTACACTGTGAACTTGTTGAGAAAAGTATGAATGAAAAAG
AGTTGGAAAGAGGTGTTATTGGTGAAAATGAAAGATGTGTTGTTTCTGCA
CCAAGTGGTAAGAAAACAGTGAAAAAAGGGTGTGTGCAGGAGCAGCTTAA
TACCTATTTACCCATTGCTTGTCGTACTAGGCAGAAGGCATCAACTCAAA
CACAGAGGGATACCAAAACATCCACCATCTTAGTAAAGGGGATAGATAAT
TTAAGTGCCCCTATTGCTCCTAAAAGGAAAAGGAGCAGGGCTGCAATAAA
CACACTTGCCCGAAAAAACTGTACTGAATTTAAATTCATCCAACCTGCAC
ACCCTAAGAAAACAAAAGTAACACATACTGAGCCATCAGATTTAAAGATA
TGTGGTACTGGTACAATCCAAAAGGGTAATGTTCATCCAAAGCCGTCCAA
GGAGGAGATAAACGGGATGTCAAATTCAAAAATAAAGTTCACACAGATGA
AATCAGGCATAAAGAGGAAAATACTGGCATCTGGGCAGTCGCAAAAAGAA
TTGGTGGAGGATACTACTGTGGACCATAGTCCTAACCACCACAGTGCCAT
TGGAACATCAGAAGATTCAGCTGTGATTAAGGCTCAGACAGCTGAATATA
CTATTGGAAAGAGTCGCTCAGCTATTACTGCTGCTGAGGGAGCAAAATTG
AATCCATGGTCTAAAGAATCACTAAAGGACATATGTACACCAAAATCTTC
AGCTTATGTGACCCCTAATATATATACAAAATCTGTAAACAAAGCCTCTC
CAGTGGGTAAAGAATATCACAAACCGTCATTCAGGAAAAAGCCATTAAGT
TCACCACTAAAGAAAGAAGATAGCAGAATAATTGAAGATGGGCCAGAATC
TGCATATACATATAAAGAGTTGCGTAAGAGGAGAGATATAACTAAAGTTC
AAGTCTTGTTCAGTCAGCACTTGGATGGAGATGTAATTAAGCAGCAGAAG
AAGGTATATTTGTATTCTTTTCTCCTCAATTCGTGATTAAAGCATATTCA
AATTTGAAAATTTATTCAATTGACTATCACAGGTTTTGTTCCGGTTGGGA
GCTTCTGTAGCATCGTCAGTTTCAGATGCTACACACTTCGTAGCTGATGA
ATTTGTGCGCACAAGGAATATGTTAGAAGCTATTGCCTTTGGAAAACCAG
TGGTGACACACTTTTGGCTAGAGAGCTGTGGACAGGCTAGTTGTCTCATT
GATGAGCGAAACTACATACTGAGAGATGCTAAAAAGGAGAAAGAGATTGG
CTTCAGTCTGCCAGTTTCACTGGCACGTGCATGCCAACATCCACTTTTAC
AAGTATGAAGTATGATCTTAGTTTATCCCATTGTTTGATGGCGGTTATGG
TTATTCTTTAGTAAACTACTATTTTTCAGGGGTGTAGGGTCTTCATCACG
CCCAACACAAAGCCTGGTAAAGATATATTGGCAAGTTTAGTGAAGGCAGT
TCATGGCGTGGTAAGTATTTTTCTAAGGATATCCCTGCAGTTATTAATCA
TTAGCACTAAACTATTGGACTCCCTGAATTTCTTTTATGTAATTTGCAAA
ATGTCAGAATTGTAATTATTGGCCATAAATTATCACTTGTTTTCATGAAT
GGCTTGGTAGTCAAACATGTAAACTGGGACTTTCTCTGCTCTTTTCTTCA
ATAACCTTGACATTAATTCACATGCATCTTTGTATGTGTATACAAGTAAC
ATATTACCCAAAGGTCAATCATAAAGCCTAAAGATATATCCTAAATGTCA
ATTTTATTAAAGCCTAACAATCTAGTTTAAAGGTGCTAACAAATTTTGCT
AAAAATTTTCTACTTTATGTATGGAGCTGACTAGTAATAAATCATAATTA
CTTCAGTGAAAACCAACTTCAAACTTGTGTTCATTGATGTTAGTGTTCTA
CCACCTAAGATTTTTGATAAGTTCGCTGGTACGATTTGTAAACCATATTT
CACTGGTTGCGGAGTAAAATTTGTATCCATTTGCTTCTTTCCTCCAACCG
CTGGTTCCTTTACGAGTCTCATATAGTCATATTTTACATGCAAGTGTGAG
AATATAAAAAGGAGCCTTTTACATTCTGCAGATAGTGTATAGTACATAGA
CCCTGCATAACGAAGTCGTCAAAGTCCTGGACTCCTGGTTACAGTTCAGC
ATAATGTGAAACTTCATTGCTGTTTTTTGAAGTACACTTGTTGATATCAG
AGCACTAAGTAATGGCTTTGGATATAATTTCAAACTTGCATGACCTCAAC
TACTAAAATTCATGTCTCCTGGTGCATGGTTAATACGTAAAACAAATGAC
CCAAGACCAGACGAATAAGATTTTGCGTTCACGAGTCTTTTCCTCTTAGG
CTTTGTTTGTAAATTGAAGAATATCGTCATACCTCAAGTAGATCAAACAT
TTTATGACTAGAAGTTGGAAGATTGGTAATGGATTCCATATATTTTCATT
GTACTGAATGATGATACACCCAATTTCAAGTTGCGTGGCGGATTCAGATA
CTTTTTGTTCATCTGGGTTTTTTAAGTCCACTGATATTTTTGTTCAGGCT
GTTGATAGAATTGGTCGATCTGCAGCAAAGGATGAAATGGTTTCAAGTGA
TTGGATCGTTCTTTCGTGTGAGGAAGATTATGCAGATTGTGTGCCCTTCC
TTGAGAAAGGTTAGGACCTATAAATACATGAGATTAATCTAGTAATAAAG
AACATTACTTTTATTTGTAATAGCAATATGTCACTCAATGTTTATATACA
GGAGCATCAATTTACAGTTCCGAGCTACTTCTAAATGGTATAGTTATTCA
AAAGCTGGAACATAAAAGGTCCCTTTCTTTTCCTTATATAAATTTTTTTA
TTCATGTTCCTGTTTCTTTGATATTAACTCGGTCTTATGCCAAAGAAAAA
AAAAAGTTGTTTCATAGGTTTCATGCGATCTGA