DCAR_025574 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_025574

Identifier:

DCAR_025574.gene

Locus Tag:

DCAR_025574

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 2 relationships.
Relationships
The mRNA, DCAR_025574, is a part of gene, DCAR_025574.
The genetic_marker, MK009737 7_28370561, is located in gene, DCAR_025574.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_025574	DCAR_025574.mRNA	mRNA	DCARv2_Chr7:28370341..28378669

Sequence:

CAAATTTGTATAATTTTCTAAAAGCTTTGGACCCTCAAGTGCAATAGGGC
TTGAAAGCAAAAATTGTTGCAAATTGCAAACGCTTCCGCAAGCAAAACCA
CGTAGAAGCACAATAGATTGCCAGGCAGGCACAAAGCTAGAGTCTTTAGC
CCCTATATAAACCCTCCACCGAAACACACATTTGTTTATATCGCTTTCAA
GAACACTTCTCTCTCTGTCCAAACCTTGTATCTAAGTTCTTAATCTTAAA
TCCGACAAGGGTTTCTTGGGGTTCGTGTCCAAATGGCTTCTTCTCCCACC
CTTCCACCCCCTCCATCCATCTCAGAAGAAGAGCTTGAAGAGGAGCTTAC
AGATGTTGGGAACAAGCTGTTGAAACCCCCTTCTTCAACTGATGAACTCT
TGAAGCTTCTTGATGTGAGGCCCCTTTTTCTTGTTTTTCATCATAAAGAT
TTGATTTTTATGTGTGCCCTTTTGGGATTAATTGATTTTGTTGGTCTGCT
TGTTATTTGTTTCTCATTTCGTGATGAATTGATGTTGGTTTCTCTAATTG
GGTTTGTTTCTTGTGAAAAGTTTGTGATTTTTAGCATAAAGGTTTGATTT
TTGTTTCTTTAGGTTTGGATTGATTTGATTTTGCACATATATGATTTGTT
TGTGAATAGATGTGGTGGTGAATGTTGTTCAATTTTGGTACAAGTACTCT
GAGGATTCGTTCATGTCATGGCGTGTTGATTGATTTGTCTAGTAATGCCG
GGCTGGAGTGATAGAATGCTATGCTAACATGACTTTCTTTTTATAAAACC
ATGTTGTTACTATACATGTATGTGCGCGCGCACCCCCACTCACCGCAAAC
ATGTACCATGTGATGTTTAAGGGTTTATTTAGTCGGGCTTTTATGACGAC
TGTCTTGTGGTGCGATATGTTACCTTTGCAAACCTTAGGGTTTTATATGT
TTAATGTACGGATAAGTATTTCCGCTGACCTAAAATGATGTCATAGCTTT
CTGATTTTTGTTGAATATATGTTACAACTCATTGGCATATGGGTGCAGAA
AGTGAATCGAATATCTGGTAAATATTTAGTTGTTCAAGTATCGGTTGCTT
CCTATTTGTTGGGATATTTCTTTTGAATATAGTATTATGTAAACCAAAAG
CTTACGGATGTCTCTGTTATCTATAATTAGAAGAAATATTAATGCTATTG
CGTAACATTGTATATACCACCAGTATACAAATTTTACAGTTTGTAACACA
CGTTATACTTTCTTGTCATGTACGTCTCTCTCTCTCTCTCTCTCTCTGCC
GTTTTTCCTTTTTGCTACATGTCTCATTATAGTCTTTCCTTTGCATTTTC
ATCACATGTTATGCTTGTTATGTTGACAATTTGTAACCTTAAATCTGCAA
TATTTCAGTTTTCGACCTTCATTTCCTGATATTGGATTATATGATCATTT
TCTAATATTGGATTTATGATCACTTGTTAAGCACTCTTTATCATAGCTTA
TACACCTTATTGACATTTATTTATTCAAACCATCTTCCAAGCTATCTACA
AGAATTCCTGCATAAACATAGTACATATTACATTCTCTGGTTTCATGCTG
TTATTTTAGGAACTCGGATGTCTCTTGTCCAAGGTCAGTCAAGAGCCAAC
TCTTTCAATGCTCGATGCTCTTCAGCCTTCAATGAAGGCGCTAATGGGTA
CTGAAATATTGATGCACAATGATACTGATGTAAAAGTTTGGGTTGCATCT
TGCATTAGTGAAATTGCAAGGATTATGGCGCCAAATGCACCTTACAGCGA
TGATGAAATGAAGGTACTTTAATTTCTAAAATAAAATTACTTTTTTTACA
AAGTTAGATGGACTTCTTGTTTTATTATTCGTGGTTCATATTTTTCCTGA
AATCATACAAATTGATGTAATTTTACGATATTACATGTTGCAATTCTCAT
TCTATTGCTCTACAGAAATTTTTTCAGCTCAGCATTGTAGCAGTTGAGCA
ATTATCCAATACCAGTGGTCGTAGTTACTCCAGAGCCGTTTTAGTTCTTA
AAACCCTTGCAAACATAAGGCTCTGTGTTCTAATGTTGGACCTTGAATTG
GATGATTTAATACTTAAAATGTTTGAGCGGTTTCTAGACATCAAGAGGTA
TGTATTGTAACCATGCTTTGTTACATGTAGAGTATCAAAAATATTTTAAT
CGTATCATATAAATTCTTGCTCCCCTTTCAATGTGCAGCCTACCTGATGC
TCTATTATCAGATATGGAGAGAATCATGACCTTGATTGTAGAGGAAAGCG
AAGAGCGTTCATTTGAACTTATGAGCGTTCTTCTAAAAAGTGTGAAGAGG
GAAAATCAGGTAGCAATTTATCATTCACCTGGGTTATATGAGCATGAGCA
TCCCTTTTATTCATTTTATTTATTTATTTTCGCTTGGGGTTTTCAGTATG
TTTCTCCCGTTTCATGGGAGTTGGGGCAGAAAGTCTTGAAAAACTGTGCT
GGCATACTCGTATTGGATATTCAAAAAGCAGTCAGCACCATGGGAATGGC
ATATACTGAGTTTTCAGAAATTGTTGCTTCAATATGTCAAGTGGCGCCTA
AAACAGACAATGTGGTAGGTGTTCTTTTGACCTATTTCCGTTTATGCCAA
TTCTGAATTAATATCTGTCTAGACTATATGGAGTGCTGGATCTATCTGCA
GTGATTGCAAATGTCGTCCTACTTTGATGTGTATGTAGAAATTATTTTTT
GCCTTAATTCTTTTCTGTTGGTTTATTTGCAATTCTGTTGATTTTTCCAT
GCTTTTGTATTTCTAGTAGGAGGCGTTGTGTTGAGTAAATTTGAAAAATG
GCTCTGACCTTTTAAACCTATATCGTACTATCGTGGGACTTCGCTTCACT
CTCAGTTTGGACTTTGAGTTTGACCAATACAGATAGTTCACAATAACATG
CATGCTTCCACAGCCATTTAGTCATTAGAATGTAATGACCTTGTCTTCTG
TTCTTACATCTGTGGCCACTAAATTGCTCAAACTCAAATTTTTTTTTACA
GGATACTCAAACATTTGTATCAGATGCCATCAGTCTGATGAAAAATGACG
AAAATGCAGAAGAACCAAGATCTGGCATGATTAGTGGCGGATCTGACACT
CTTGTGCATCAGATCGCTTTAACCAATTGCCAACAGATAAGTGTCGCACC
ACTAAGCAATTCCCAACCTAAAGATATTAGTAAAGGAGCCGTGGCACAAC
AATCTCAAAGAAAAAGGGGTAGAAAACCCAATTCTCTGAAAAAAGAAGAG
GAGGGATATGACAATTCATGGGTTATTGGAATAAGCAGCTCTAATAAAAC
ACCTTGTCGTGGGAAGAATGCCAGAAAGAGAAGTATTCCATCTAATAGTT
CAGCTTTGGCAGGTTCATTTTCTCCATCTGAACCTGGCAAAGAACCCAAA
TCTCTTGCCTTTTCAGTCAATAATGTACAAGGTGTGAGTCTACCTTTGCC
ACCGGTTGAGAATTGTAGCACTCCAGAGAATGTTCACTCTAGACCTCAAT
CTGGTGTGCAACAGAAAGAAAAATCGAATAGTTCAATGAATGCAGACAAC
GGCCTCAATCTATTGTCAGTCTCTGCAGGAGATCTGGTCAAAACCCAGAG
TGAAGGAACGCCAGTGAGAGTAGCTTCTAAAGTATCTGGAAGCACTGGGA
ATCCCCGGGGAAGGAGAAAGAGAGGTCCGGTAAATACTGCATCTCAAGGT
GATGGGAAAGCAAAGCGTAAGAGGGCCGCAAGGAAAAGTGAAACCGGAGA
TGCTGCTGTGAAAAACAGAGAAGGTATTATTCCTTCTACGACAGATGGTA
TTGGCAGTTCTCAGGAAATAAAGACGGATTTTCCAGTGTTGCAACTTGAT
GCTGGTGTACAAAAACTAGGCAGGAGTGCTCCCATTGGGCATGCTACTGA
TAAAACTAGTGATGAGTTTGCATATGGTGGGGCATCAAAGGTATGTTGCA
ATAATAATTTGGAAGTGTTGTGCCATTTAATGTTTATTTTTGGTGTCCTG
TGTTGTTAACACCAATAAGTTCTTAGTTGAACAATTTTTATGAAGTTCAT
TTTCTTTGTTTTTTATAAACGACTTTTCCATGCTATTATCAAATAACACA
TGGAATTAGCTGTATATGACTTTTCCATGCTATTATCAAATAAGAAATAA
CACAAGGAATTAGCTGTCCATATTAACACATCAGCAGCTCATATTTTGTA
TACTCATTCATAGCCATATTATACCTACTGCTTAGTATTGCTTCTGTCAG
CAAACAATTTCACAAACATTTTAGCCACCTATTTGAATCAAACTAGTGGT
TTGTTACATTATATTCCTGAGATATATGCTTTGTGCATTCTAAGACTCTT
CGCAGAATGGATTTCTTTTAACAATACGTTCCTCGGTGCAAACTTAGGGT
CAGCTGTTAAATAATGATATCTACTTTTTAAGCAAAACAAAAATTCTGGA
TAGAAGAAGCGACGTAGGGTGTATCATATAAGGAACGAAGATCAAAATCG
TTGTAGTAACTATACTCTGAAATATGAGAAAGCAGCACTGTTAGCCGGTG
TGAAATTATGTGGTGATGTGTAATATAGATGCCTCATCACAATTTTTATA
TTTGTAAGAATGTTGTAAAGTTGTATTTTGTAGCTTAGTTTTGATTAAAA
TGTTCCTTCTCAGGATCATGGTGAAGAGTTAGTTGGTCGCAAGATAAAGG
TTTGGTGGCCAGCAGATGACCAGTAAGTACTAATGATCATCCCTCCTGCC
TCCTTAAATTTTGTGACGTTTTTACAAGCCGCGTATGTTCTTCGTGATAT
TAACAGTTGTATCCTCTGCACATTTTTGCTGGTTTGGATTAGAATAGACA
TTATCACAAATCAGCTACAGATATGTTGGCAGCATAAGCGGTGCACTATA
TCTTTTTCACATAAATGTTGGAGAATGTGGTCTAGTCATCGACATGAACA
GAAAAGTGTAAATGTTTTGTTGCTCGTAGTAGACTTATTATGCTTCTGTG
TCCTAGAATAACCTTTGTAGCATTATTGAAAGGTTATTATTTAGGATGGC
TTCTCGTCTGATGGCAAGCTAGGTATTTAGTAATTTTAAAAATTACCCAC
GGCTTCATATTTTGTCACTCTAATAATTCTAGAAGCATGAAATTTAAAGG
ACCATGAAGCACATATGTTTCAGCAGTGTGTCCCTTCCTGGGGTTTAATA
TAAGTTTGGGTATAGTCCTTGTTGTTTGATAGATGATTAATTGTCTCTCC
TTTTTTCTTGTCTATATTCTACCTTTTAGCTTCCTGTCTGTTAAAAACTT
CTTAGGTTTAATACGTGTGAAAGTTATATGTGATATATATTGGCAGGAGT
TTATTACATGTGTTATGCGTTAATATATTGCAGATATATGTTTTAATTAA
ATTAATTTGCCTATTGTTTTGTTTTTTTAGGTTTTACAAAGGAGTTATCA
CTGGATTTGACCGAGAACTAAAAAAGCATAAGGTGCATTACTATGGGTGC
ATGCCTCTGTTAACTTCTTTTATTATTCTTTTTTGTTGGCAATGATTTCT
GTACTTCCCAAATCAAACTGACGCCAAGTGCAACCATGACCTATATGAAT
TGGTTTTGTTTTGTTTTTGCTCGATGCTCTTGGCACTTTCCCCATCTAAT
ATATAGGTGTTGACCCGTCCATGGCAGGCCCGATAGTGGTAATTGTGTTG
GTACCTAGCTCCCAAAGATGTAGTTGACTGTGTATGATTGACCTTGAAGG
AAATCCCATGATAAGACAAACAAACTACAGCGTAAAAAGATCTTTGTCCA
CATCCTAAAATCTCAGTATAGTTGATTAAATATTTCTCTATACATGTTTT
GTCTCCCTGAAGGAATTCCAGCCCCATTTCCTCAGCTCCTGCTAGGTGAT
GTGGCTGGAGATGAGAACCAGCATTAAGACCCCTCTTTATAGCATTCTGA
AAATTGGCAATACCATGGCTTGCCTTGCACCCTCTGTTTGCATTATATTT
TGGGCTTTTTTTGCCTTCTCCATTGATTTTATGCTTTTCCTGCCTTTTCC
ATTGTCACTAATTATTAGAGAGAAACTGCTTCATTTAAGAATTGTTTGTC
TTCTTAAATGAATCAGAGATTTATTGAAATCTAAAACTGATCATCAATTA
GTTGTCTGTTTCACAGGTGGAATATGTTGATGGCGATCAAGAGAATTTGT
ATCTTAGAGCAGAACGTTGGGAAATAGTTGAAGATGTGAGTGAATCATGA
TGCATATTACGTGACTTTGTCTTTGCAACTTTTTCTTATTATTTTGTTAC
TTTTTTATATAATTGGGCAATTGAAAATATGCTTTACTGATGGTAGGAGG
AAGACAGCGAGGCAACTGAAGATACACGGCCACCTTTAGTAACTTCAGCT
AACAGGTAAGCAGCTTCTAATTATATTACTTTTGCCGTTTGACTATTTCT
TAGGTCTAAAATTTTTCAAATAATTTTGGACATACTTCACATATGTATGA
ACAGAATAATTTGACTGTTGATTTTTGTAAAGAACAGAATAGTCAGTTAA
TTGTTTAGTTGCATCAGCAGTCTATTTTGTTAGCTATGTAGGTTGATAGA
TGTTAAATTCTTGTATGTATACTAGCCTATCTGGACTAGATTGGTAATAT
CTGCTCATTACCAATAATATGGACAGTTACTTCAGGAAATAGGCTCTTGA
AAATCATCACTAGTGTAATGTTCCTCATTTCAAATTAAATTTCACTGTTG
CTTGGTTTCATGGCGGATTGTTCTGTCCTAGTCTAAGTTACACCTAATGA
TGTCCAGAGATCATATTAAGCACTGCTGACTTGCCTATAATTGAAATTGC
CACTGATTTTTGGGATCTATGATATTCCTAGACCCAACTATAATTGGTAC
AAAGGTTCTGTGAATTCTGTCCCACTGTAACTTTTATTTGGTACACCATT
ATCAATTTTGAGGATTGGTATGATTTGGGAAAAATCTGTGTTTAAATGCT
GGTAATCATTATAGAAAATGTAATACATTATAACTTTGATAGACATGTGC
AGTGTGTCCACACATCCGATTATGAATTAGCATTCAGCTGTAAACTCAAT
ATTTTAATATCTTTTAAATTGATTTAGGAAACATGAATAATTTTCCTCGT
GCTTCACTTAGCAAACATAAAGTAGTTTAACTCTGTCCAGCAACTGGTTT
TCTGTAACATGCGTTGGAATTATCTGAGATCCTTCTACTAATATTTTAAT
GCCACACTTGATATAAAACTTTCAAGCTTCTAAACACCTTTCCTTTTGTG
CAACTATCTGCTATATGACATATAGCACTCTGTAATGAAACAGAATTTTC
CTATTGATTAAGAGACCAATTGAGTTCAACTAGATATATGCTGTGCCTTA
TTACGTGTTAAACGTCTAATCCTTCTTTTTTGATTTACTCGATGCAGGTT
CCCCAGTGCTGGAGCTAATTCTTCTCATCCAGGGAATCTCAGTGAAACGG
TGAGATCATAGTAAACTCTTTTTATGGAAAGTATAACAAGAAGTTGTGTT
GAGAATTTTGTACCATCATAAGATTGACTGATGATGTTTCTGTGACTGGT
CATCATATTCCTCATCTCCTATAAACATTGCAAAATTTATCTGGTTATTT
TGCACTTGTTTAATCAAAAGGAGCAAAGTGTTTAAAATTTAAGAACTGTG
GAGAAGAAAATAATGTCATCTTTTTATTAGGCTCATGTAGAAATTATTTC
TGATAATATTCTGTGAAAATCCAATGCAGGCTTCTGCTTCACATAAGCAA
GGCATGCTTGTCCGCAGTAAATTGAAGCCAGATGGGGTGAGTTGGTCCAT
TGTTTACTGTGTGATCTCTTCCATCTCTTTTATCCTGAAACTTATAAGCT
GAATGAAGAAACTCGATGCAATTGTCTTACTATTTATAATCCATTCCTTT
CCAGGCTGGAAGGACAAAAAGGAGGACAGTGCATGATGTCCACAGCAAAA
TTTCACCAGCACATGAGGCAAGTGAGCATGCTGGCGAAACAAGCAGCATC
CCAGTCACTGTTGCTGGGTACAGGGTGAAGTCTGCCAATGCACCTATTCT
AACCTCCATTATAGCGAGGTACGGTGACATTGCTGCCAAGTGCATGTTCA
CTTCAGCCACTGTCAGAGCATCAATGCTTGATGTTATATGCGACATTGTC
AAGCGGCTGCGAAGCGGCAATGCGCAGCAGACCCTATCGCGGCTCGATGC
TATGGAGACTGAGCTGCGAGATGCAGAGGCTGCCAAAATCGAGGTCTCCT
GGCTCCAACAATGCTTGACCAAGTGCCGTGAAGCTCAAGGCAAAATCCCC
TCCGTGCTCAGAGAAATGGAGTCCAGCCTGTGGCTGGTCTCCAAAGCTGC
AGAAAGGGACTTGGCAGCCGCTCAAAAGTGGGCCAAGGAGTCCGAAAGCT
GCTTAAGAGCATTAGCGGTGGTCGGGAAGAAGTTCCGCGATGAACTGGCC
CTACATGAATCCAAAGCAAGCGAATGGCGGAATCCCCTGGATGAGCTGGC
CAAGCCGTGAGCCTGTGATGTGGGTTTAGAGTAGCAAACATGTGTAGTTC
CAGAATTTGTGGATGTTGTAAAATGTTTGTCAAGTTAACAAGTCTTTAGT
TGGATGTATTTCTGAGGGT

Sequence Length:

8719

Sequence Checksum:

d61d8f84c273c0ab87c4b6d3c89368ae

View location in JBrowse:

DCARv2_Chr7:28370233..28378951-

Cds:

>DCAR_025574cds ATGGCTTCTTCTCCCACCCTTCCACCCCCTCCATCCATCTCAGAAGAAGA GCTTGAAGAGGAGCTTACAGATGTTGGGAACAAGCTGTTGAAACCCCCTT CTTCAACTGATGAACTCTTGAAGCTTCTTGATGAACTCGGATGTCTCTTG TCCAAGGTCAGTCAAGAGCCAACTCTTTCAATGCTCGATGCTCTTCAGCC TTCAATGAAGGCGCTAATGGGTACTGAAATATTGATGCACAATGATACTG ATGTAAAAGTTTGGGTTGCATCTTGCATTAGTGAAATTGCAAGGATTATG GCGCCAAATGCACCTTACAGCGATGATGAAATGAAGAAATTTTTTCAGCT CAGCATTGTAGCAGTTGAGCAATTATCCAATACCAGTGGTCGTAGTTACT CCAGAGCCGTTTTAGTTCTTAAAACCCTTGCAAACATAAGGCTCTGTGTT CTAATGTTGGACCTTGAATTGGATGATTTAATACTTAAAATGTTTGAGCG GTTTCTAGACATCAAGAGCCTACCTGATGCTCTATTATCAGATATGGAGA GAATCATGACCTTGATTGTAGAGGAAAGCGAAGAGCGTTCATTTGAACTT ATGAGCGTTCTTCTAAAAAGTGTGAAGAGGGAAAATCAGTATGTTTCTCC CGTTTCATGGGAGTTGGGGCAGAAAGTCTTGAAAAACTGTGCTGGCATAC TCGTATTGGATATTCAAAAAGCAGTCAGCACCATGGGAATGGCATATACT GAGTTTTCAGAAATTGTTGCTTCAATATGTCAAGTGGCGCCTAAAACAGA CAATGTGGATACTCAAACATTTGTATCAGATGCCATCAGTCTGATGAAAA ATGACGAAAATGCAGAAGAACCAAGATCTGGCATGATTAGTGGCGGATCT GACACTCTTGTGCATCAGATCGCTTTAACCAATTGCCAACAGATAAGTGT CGCACCACTAAGCAATTCCCAACCTAAAGATATTAGTAAAGGAGCCGTGG CACAACAATCTCAAAGAAAAAGGGGTAGAAAACCCAATTCTCTGAAAAAA GAAGAGGAGGGATATGACAATTCATGGGTTATTGGAATAAGCAGCTCTAA TAAAACACCTTGTCGTGGGAAGAATGCCAGAAAGAGAAGTATTCCATCTA ATAGTTCAGCTTTGGCAGGTTCATTTTCTCCATCTGAACCTGGCAAAGAA CCCAAATCTCTTGCCTTTTCAGTCAATAATGTACAAGGTGTGAGTCTACC TTTGCCACCGGTTGAGAATTGTAGCACTCCAGAGAATGTTCACTCTAGAC CTCAATCTGGTGTGCAACAGAAAGAAAAATCGAATAGTTCAATGAATGCA GACAACGGCCTCAATCTATTGTCAGTCTCTGCAGGAGATCTGGTCAAAAC CCAGAGTGAAGGAACGCCAGTGAGAGTAGCTTCTAAAGTATCTGGAAGCA CTGGGAATCCCCGGGGAAGGAGAAAGAGAGGTCCGGTAAATACTGCATCT CAAGGTGATGGGAAAGCAAAGCGTAAGAGGGCCGCAAGGAAAAGTGAAAC CGGAGATGCTGCTGTGAAAAACAGAGAAGGTATTATTCCTTCTACGACAG ATGGTATTGGCAGTTCTCAGGAAATAAAGACGGATTTTCCAGTGTTGCAA CTTGATGCTGGTGTACAAAAACTAGGCAGGAGTGCTCCCATTGGGCATGC TACTGATAAAACTAGTGATGAGTTTGCATATGGTGGGGCATCAAAGGATC ATGGTGAAGAGTTAGTTGGTCGCAAGATAAAGGTTTGGTGGCCAGCAGAT GACCAGTTTTACAAAGGAGTTATCACTGGATTTGACCGAGAACTAAAAAA GCATAAGGTGGAATATGTTGATGGCGATCAAGAGAATTTGTATCTTAGAG CAGAACGTTGGGAAATAGTTGAAGATGAGGAAGACAGCGAGGCAACTGAA GATACACGGCCACCTTTAGTAACTTCAGCTAACAGGTTCCCCAGTGCTGG AGCTAATTCTTCTCATCCAGGGAATCTCAGTGAAACGGCTTCTGCTTCAC ATAAGCAAGGCATGCTTGTCCGCAGTAAATTGAAGCCAGATGGGGCTGGA AGGACAAAAAGGAGGACAGTGCATGATGTCCACAGCAAAATTTCACCAGC ACATGAGGCAAGTGAGCATGCTGGCGAAACAAGCAGCATCCCAGTCACTG TTGCTGGGTACAGGGTGAAGTCTGCCAATGCACCTATTCTAACCTCCATT ATAGCGAGGTACGGTGACATTGCTGCCAAGTGCATGTTCACTTCAGCCAC TGTCAGAGCATCAATGCTTGATGTTATATGCGACATTGTCAAGCGGCTGC GAAGCGGCAATGCGCAGCAGACCCTATCGCGGCTCGATGCTATGGAGACT GAGCTGCGAGATGCAGAGGCTGCCAAAATCGAGGTCTCCTGGCTCCAACA ATGCTTGACCAAGTGCCGTGAAGCTCAAGGCAAAATCCCCTCCGTGCTCA GAGAAATGGAGTCCAGCCTGTGGCTGGTCTCCAAAGCTGCAGAAAGGGAC TTGGCAGCCGCTCAAAAGTGGGCCAAGGAGTCCGAAAGCTGCTTAAGAGC ATTAGCGGTGGTCGGGAAGAAGTTCCGCGATGAACTGGCCCTACATGAAT CCAAAGCAAGCGAATGGCGGAATCCCCTGGATGAGCTGGCCAAGCCGTGA

Pep:

>DCAR_025574pep MASSPTLPPPPSISEEELEEELTDVGNKLLKPPSSTDELLKLLDELGCLL SKVSQEPTLSMLDALQPSMKALMGTEILMHNDTDVKVWVASCISEIARIM APNAPYSDDEMKKFFQLSIVAVEQLSNTSGRSYSRAVLVLKTLANIRLCV LMLDLELDDLILKMFERFLDIKSLPDALLSDMERIMTLIVEESEERSFEL MSVLLKSVKRENQYVSPVSWELGQKVLKNCAGILVLDIQKAVSTMGMAYT EFSEIVASICQVAPKTDNVDTQTFVSDAISLMKNDENAEEPRSGMISGGS DTLVHQIALTNCQQISVAPLSNSQPKDISKGAVAQQSQRKRGRKPNSLKK EEEGYDNSWVIGISSSNKTPCRGKNARKRSIPSNSSALAGSFSPSEPGKE PKSLAFSVNNVQGVSLPLPPVENCSTPENVHSRPQSGVQQKEKSNSSMNA DNGLNLLSVSAGDLVKTQSEGTPVRVASKVSGSTGNPRGRRKRGPVNTAS QGDGKAKRKRAARKSETGDAAVKNREGIIPSTTDGIGSSQEIKTDFPVLQ LDAGVQKLGRSAPIGHATDKTSDEFAYGGASKDHGEELVGRKIKVWWPAD DQFYKGVITGFDRELKKHKVEYVDGDQENLYLRAERWEIVEDEEDSEATE DTRPPLVTSANRFPSAGANSSHPGNLSETASASHKQGMLVRSKLKPDGAG RTKRRTVHDVHSKISPAHEASEHAGETSSIPVTVAGYRVKSANAPILTSI IARYGDIAAKCMFTSATVRASMLDVICDIVKRLRSGNAQQTLSRLDAMET ELRDAEAAKIEVSWLQQCLTKCREAQGKIPSVLREMESSLWLVSKAAERD LAAAQKWAKESESCLRALAVVGKKFRDELALHESKASEWRNPLDELAKP*