DCAR_025630 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_025630

Identifier:

DCAR_025630.gene

Locus Tag:

DCAR_025630

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 4 relationships.
Relationships
The mRNA, DCAR_025630, is a part of gene, DCAR_025630.
The genetic_marker, MK009739 7_29170500, is located in gene, DCAR_025630.
The genetic_marker, MK009738 7_29170828, is located in gene, DCAR_025630.
The genetic_marker, MK004107 K1551, is located in gene, DCAR_025630.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_025630	DCAR_025630.mRNA	mRNA	DCARv2_Chr7:29169339..29176509

Sequence:

ATGGCGACAGTAGGAGCATCGGGATGGTTGAAGGGAAAAGTCAAAGCTGT
TCCATCTGGGGACTCTCTGGTTATAATGGGGAACACAAAGGCTGAAATTC
CTCCCGAAAAGACCATAACCTTGTCATCTCTTGTTGCTCCAAGATTGGTA
ATATTATACAGGCTTTAAAGCTTAAATCTTTATATTACTTTTTAGTTTAT
GATATAAATGGTATAAGACTATGATATATTAACAACACTTGGTTTGCAAG
CATTTATTTATCTTCCTCATTATCATTGCAGGCTCGTAAAAACAGTGATG
ATGAACCTTTTGCATGGGAGAGCAGAGAGTTTCTAAGGAAGCTTTGCATA
GGACAGGTACTTGGTTATCAGTTACTTGCTTTTCTTTTTTCAGTTCACTA
CTCGTATAAGCATTAAACCCTGCTCAATTTTGGTTCACTAGGAGGTAATT
TTTAAAGTGGACTATGAAGTACCAGCCATAGGCCGAGAATTTGGTTCTGT
TTTTATTGGTGATAAGAATGTTGCGTGTCTCGTTGTTGCTTCTGGATGGG
CAAAGGTCAGATTTTTATTCTTTCTTAGGTTCTAATAGATGATAATTAAT
AATGCTTCTAGCTAATACTATTGGTCAAATCTTTATTTACAGGTTAGAGA
GGTTCGGGAGCAAAGTCAGCAGAAAGCTGAAGTCAGTCCTTTTGTAGCAG
AACTTTTGCGATTAGAAGAGCAAGCCAAACAACAAGGTGTTGGTCGCTGG
AGCAAGGTTAGTACAATATCTGCATCATAAAATTGCTAAAGGACTGGTTA
TTGGCAATGTCATATAGTTTGATTTCATATCTATATGTATCTTTATGTAT
GGGGTTGTGTAAGAATATTCTTAGAATTATATTCTGCAAACTTAAAATTT
TATGTACTAAAACAGCTGATATATTTGTGTTATGTGTCCTAGCCTGTTGG
TGTTTTCTACATTATTAAGGTGTCTTTGAATACAAATTTACAACGCATAT
GCCTTTTGGTTGTCTCTTTGACTATAAACTTTTTTGGACCTTGGCTGTTT
AATTCTTTTATTAACAATATACTTCTTCATATGCAAAACATTCTGACACA
GAAAAATCACTTGTAGGTGCCTGGTGCTTCTGAGGCAGCCATCAGAAAAT
TGCCTCCTTCTGCAGTTGGTGATCCTAGTAACTTGGATGCAATGAGCCTT
TTAGCTGCCAATAAAGGTAGGCCCATGGAAGCTATTGTGGAGCAAGTTCG
TGATGGAAGTAGTCTTCGTGTTTTTTTGCTTCCAGATTTTCAGTTCGTCC
AAGTTTTTGTTGCTGGTATTCAGGTAGAATCCAATCTTGACCTCAACATT
TTTCTAATAGGTGTCATGTTTAAAAGTAATGTTCACGTTTATTTCTTTTT
TGATATTACAGGCACCCTCTATGGGTAGAAGGGCTGTGCAGGACCCAATT
CCTGAACCTGAAATGACCGCTGCAGCTGATGAAAATGGCAATGCTTCTAC
TAAATCTCGTGGCCCATTAACATCGGCACAGAGACTTGCAGCATCACCAG
CCTCGTCTAATGAAGTTACCCCAGAATTATTTGGGCGGGAAGGGAAACAC
TTTACAGAGATTCGTGTATTACATAGAGATGTGAGTGCTGTTTCAAACCG
CCGTTATTTACTGTTGTTTACTTTAATGGCTCTAATTTCATGATTTAAAT
TATAGGTCCGCATTGTTCTGGAGGGTGTTGACAAATTCAGCAACTTGATC
GGTTCCATATATTATCCTGATGGTGATGTTGCGAGGGATTTAGCTATGGA
GCTTGTTGAACATGTAAATTTTCGACATAAAACATACATGATTAAAATGA
AGAAACTAATATTACTATTTCGTTTCAACACACTAATTGTATAAAGTCCT
TTTAGTTGACTTCAATCTTCTTTTACTGTCTTTTTTATTGAATTCAGGGC
TTAGCTAAATATGTTGAATGGAGTGCTAGTATGTTGGATGATGAGGCCAA
ACGGAAACTAAAAAATGCTGAACTTGAAGCTAAAAAGAGTCGGTTAAGAA
TGTGGACAAGCTATGTGCCTCCGGTTTCTAATTCGAAGGCAATTCACGAC
CAGAATTTTAGTGGAAAGGTTGGTTTTTGTTTATCCAAGTTCACGTGTAA
TCATTTGATGTTTACAAAACTTTATCCTTGTCACCTTTAATTTGATTTTT
TGTTTTTTAGGTTATTGAGGTCGTGAGTGGGGATTGCATTGTTGTAGCAG
ATGATGCTCTTCCATTTGGCAGTCCATTAGCTGAGCGAAGAGTAAATCTC
TCAAGTATAAGGTGTCCGAGACTAGGCAATCCTAGAAAAGATGAAAAAAG
CCCCCCTTATGCTCGTGAAGCGAAGGATCTCTTGCGAACACGTCTAATCG
GCCGTCAAGTATGGACATTACTAGTGTTCACATCTAAAAGAATCTATTTT
GAGTGGCTTTGATTTATTTTGAATAAACATTTTTTCAGGTACATGTTTCA
ATGGAATATTCTAGGAAGGTAAACTTGGCAGAGGGACCTGGTGCTCCAAC
TGGAACAACAGATAGGGTAATGGACTTTGGATCAGTCTTTTTGCCGAAGG
ATGGCGAGGCTCCACCTAGTGCAGGCAGTCAGCCGACAGGGATTAATATT
GCTGAGTTAATTATTTCTCGCGGTTTTGGTGAAGTCATTAAACATCGTGA
TCTTGAGGTAAGATCAAATTACTATGATTCCCTTCTTGCTGCCGAATCGC
GTGCCAAAGCTGGGAGGAAAGGGATGTATTCCGGCAAGGATGCTCCAGTG
ATGCACGTCAATGACTTTATAACGGTAATAATAAGACATAATCCATTATT
AATGCTTGGAATGATTCATATTATTAACTTTCCACAATAAAATCTATTGC
AGGCTTCATCTAAAAAAGCGAAAGATTTCTTGCCATTTTTGCAACGGAAT
AGAAGGATGACAGCTGTTGTGGAATATGTGTTTAGCGGTCACCGATTTAA
ATTATATATTCCAAAGGAAACTTGCAGCATCGCTTTCTCGATCTCAGGAG
TGAGATGCCCAGGACGAGATGAGCCTTATTCCAGTGAAGCGATTGCACTA
ATGAGAAGGAAAATCATGCAGAGGGATGTTGAGGTAGTGCATAATGTTTT
TATGATTATTTATGTCCTAGTTAATGTTCATAAATTCTCTTTATAGTCTT
TGAATAAATGCTTTCTCTTTTGCAGATTGAAGTAGAAACTGTTGACAGAA
CAGGTACTTTTTTGGGATCTTTGTGGGAGTCAAGATCCAATGTCGCATTG
ATACTACTCGAAGCTGGTTTGGCCAGGCTTCAAACCTCTTTTGGTACAGA
TAGGATACCTGATATCCACCTGCTTGTGCAGGCTGAGCAGTCTGCTAAGA
AGCAGAAATTGAAGGTGAGATTGAGAATTGTTATCCTAGCACAACTATCC
CACTTTCTAATGTTTCTTATAGTTTCTTTTTCTTCTTGTTGAAGATTTGG
GAGAACTATGTTGAAGGAGAAGAAGTTTCTAATGGCCCAACTTCTGAAAG
AAAGAAAGAAGAATTTAAGGTATAAATTTAATATTTGATCAATTCAGCTA
AGTACTTGTCAGATGATCAATAAAGTTGTGACATAGTTTACTTGTATATA
CTCTCAGGTAGTGGTTACTGAAGTCCTTGGCGGTGGAAAGTTTTATGTTC
AGCCAGCAGATCAGAAAGTGGCATCCATTCAGCAGCAGCTTGCTTCTTTG
ACACTCCAGGAGGCTCCTTTGATCGGTGCTTTCAATCCAAAGAAAGGCGA
CCTTGTCCTTGCACAGTTTAGTGCGGATAATTCCTGGAACCGAGCAATGG
TGAGAGTGACATCATAATACCACACTTTTTGCTCCTTTTTCTTTCCTTTA
TCTGCATGCATAGTGCAAAATTATATCTTTGTTATTTCTATGATGACTTT
CTAAAATTTTCCTGTTATCCAATACCTGAAGATTGTGAATGCCCCTCGAG
GTGTCGTTGAATCTCCAAAAGACAAATTTGAAGTGTTTTACATTGACTAT
GGAAATCAAGAAATGGTTTCTTTCAGCCAATTGCGACCTGTTGATTCTTC
CGTGTCTTCGTCACCTGGCCTTGCGCAACTGTGCTGTCTTGCTTATGTAA
AGGTTCCAAGTTTGGAGGAGGATTATGGGCAGGAAGCAGCTTTTAGTCTG
AGCGAGCACACACTTAGTGGGCCAAAAGAGTTTAAGGCTGTTGTTGTAGA
GAGGGATGCGTCTGGCGGAAAAGTCAAAGGACAAGGAACTGGTACTGTCC
TTATGGTAACTTTGATTGATGAGGAGGCTAATGAAAGCATAAATTCCCGC
ATGTTGAAGGTATGTTTTTATCTCCTGTGGCTAATTAAGAATACAAACAT
GTAAACTTTTTCTTGTGAAATCCCTATCCATGGGATTTTCAATGTGCTTA
CCTTCTTACAAACTTGTTTCAAAATTATATATGGATAGTCAACTGATATT
TTTACTTTTTACAATGTATTTACTCTGGGGGTTTTCCATTCCACAGCTAA
ATTATTAAGCTATGGCATCTCTTCTACTTTGTATTGCATATTGTAAGGGA
AGACCTCCCTTCTGTATGTGGACATTCTCTTCCAATAATTGACATGTAAC
ATTTTGCAGGAAGGACTTGCTAGGTTGGAGAAGCGGAGGAGATGGGAGCC
GGTCGAAAGAAAACAGGTGATTGACGAATTAGAAAAAGACCAAGAAGAAG
CTCGTACTAAGAGGCTGGGCATGTGGGAGTATGGAGATATAATGTCAGAT
GACGAGGACAGTGCTCCAGTAAAGAAACCCAGTGGTGGCAGGAGGTGAAT
ACATGGTTTTCTAGTAACCTAAATAATTTAGGTGAGAACAACATAGGATG
AAAAGTGCTACAAGAAANAAGGATGGCGAGGCTCCACCTAGTGCAGGCAG
TCAGCCGACAGGGATTAATATTGCTGAGTTAATTATTTCTCGCGGTTTTG
GTGAAGTCATTAAACATCGTGATCTTGAGGTAAGATCAAATTACTATGAT
TCCCTTCTTGCTGCCGAATCGCGTGCCAAAGCTGGGAGGAAAGGGATGTA
TTCCGGCAAGGATGCTCCAGTGATGCACGTCAATGACTTTATAACGGTAA
TAATAAGACATAATCCATTATTAATGCTTGGAATGATTCATATTATTAAC
TTTCCACAATAAAATCTATTGCAGGCTTCATCTAAAAAAGCGAAAGATTT
CTTGCCATTTTTGCAACGGAATAGAAGGATGACAGCTGTTGTGGAATATG
TGTTTAGCGGTCACCGATTTAAATTATATATTCCAAAGGAAACTTGCAGC
ATCGCTTTCTCGATCTCAGGAGTGAGATGCCCAGGACGAGATGAGCCTTA
TTCCAGTGAAGCGATTGCACTAATGAGAAGGAAAATCATGCAGAGGGATG
TTGAGGTAGTGCATAATGTTTTTATGATTATTTATGTCCTAGTTAATGTT
CATAAATTCTCTTTATAGTCTTTGAATAAATGCTTTCTCTTTTGCAGATT
GAAGTAGAAACTGTTGACAGAACAGGTACTTTTTTGGGATCTTTGTGGGA
GTCAAGATCCAATGTCGCATTGATACTACTCGAAGCTGGTTTGGCCAGGC
TTCAAACCTCTTTTGGTACAGATAGGATACCTGATATCCACCTGCTTGTG
CAGGCTGAGCAGTCTGCTAAGAAGCAGAAATTGAAGGTGAGATTGAGAAT
TGTTATCCTAGCACAACTATCCCACTTTCTAATGTTTCTTATAGTTTCTT
TTTCTTCTTGTTGAAGATTTGGGAGAACTATGTTGAAGGAGAAGAAGTTT
CTAATGGCCCAACTTCTGAAAGAAAGAAAGAAGAATTTAAGGTATAAATT
TAATATTTGATCAATTCAGCTAAGTACTTGTCAGATGATCAATAAAGTTG
TGACATAGTTTACTTGTATATACTCTCAGGTAGTGGTTACTGAAGTCCTT
GGCGGTGGAAAGTTTTATGTTCAGCCAGCAGATCAGAAAGTGGCATCCAT
TCAGCAGCAGCTTGCTTCTTTGACACTCCAGGAGGCTCCTTTGATCGGTG
CTTTCAATCCAAAGAAAGGCGACCTTGTCCTTGCACAGTTTAGTGCGGAT
AATTCCTGGAACCGAGCAATGGTGAGAGTGACATCATAATACCACACTTT
TTGCTCCTTTTTCTTTCCTTTATCTGCATGCATAGTGCAAAATTATATCT
TTGTTATTTCTATGATGACTTTCTAAAATTTTCCTGTTATCCAATACCTG
AAGATTGTGAATGCCCCTCGAGGTGTCGTTGAATCTCCAAAAGACAAATT
TGAAGTGTTTTACATTGACTATGGAAATCAAGAAATGGTTTCTTTCAGCC
AATTGCGACCTGTTGATTCTTCCGTGTCTTCGTCACCTGGCCTTGCGCAA
CTGTGCTGTCTTGCTTATGTAAAGGTTCCAAGTTTGGAGGAGGATTATGG
GCAGGAAGCAGCTTTTAGTCTGAGCGAGCACACACTTAGTGGGCCAAAAG
AGTTTAAGGCTGTTGTTGTAGAGAGGGATGCGTCTGGCGGAAAAGTCAAA
GGACAAGGAACTGGTACTGTCCTTATGGTAACTTTGATTGATGAGGAGGC
TAATGAAAGCATAAATTCCCGCATGTTGAAGGTATGTTTTTATCTCCTGT
GGCTAATTAAGAATACAAACATGTAAACTTTTTCTTGTGAAATCCCTATC
CATGGGATTTTCAATGTGCTTACCTTCTTACAAACTTGTTTCAAAATTAT
ATATGGATAGTCAACTGATATTTTTACTTTTTACAATGTATTTACTCTGG
GGGTTTTCCATTCCACAGCTAAATTATTAAGCTATGGCATCTCTTCTACT
TTGTATTGCATATTGTAAGGGAAGACCTCCCTTCTGTATGTGGACATTCT
CTTCCAATAATTGACATGTAACATTTTGCAGGAAGGACTTGCTAGGTTGG
AGAAGCGGAGGAGATGGGAGCCGGTCGAAAGAAAACAGGTGATTGACGAA
TTAGAAAAAGACCAAGAAGAAGCTCGTACTAAGAGGCTGGGCATGTGGGA
GTATGGAGATATAATGTCAGATGACGAGGACAGTGCTCCAGTAAAGAAAC
CCAGTGGTGGCAGGAGGTGA

Sequence Length:

7170

Sequence Checksum:

67fcb29c4ce6b3042b82ace75e3f87d9

View location in JBrowse:

DCARv2_Chr7:29169340..29176509+

Cds:

>DCAR_025630cds ATGGCGACAGTAGGAGCATCGGGATGGTTGAAGGGAAAAGTCAAAGCTGT TCCATCTGGGGACTCTCTGGTTATAATGGGGAACACAAAGGCTGAAATTC CTCCCGAAAAGACCATAACCTTGTCATCTCTTGTTGCTCCAAGATTGGCT CGTAAAAACAGTGATGATGAACCTTTTGCATGGGAGAGCAGAGAGTTTCT AAGGAAGCTTTGCATAGGACAGGAGGTAATTTTTAAAGTGGACTATGAAG TACCAGCCATAGGCCGAGAATTTGGTTCTGTTTTTATTGGTGATAAGAAT GTTGCGTGTCTCGTTGTTGCTTCTGGATGGGCAAAGGTTAGAGAGGTTCG GGAGCAAAGTCAGCAGAAAGCTGAAGTCAGTCCTTTTGTAGCAGAACTTT TGCGATTAGAAGAGCAAGCCAAACAACAAGGTGTTGGTCGCTGGAGCAAG GTGCCTGGTGCTTCTGAGGCAGCCATCAGAAAATTGCCTCCTTCTGCAGT TGGTGATCCTAGTAACTTGGATGCAATGAGCCTTTTAGCTGCCAATAAAG GTAGGCCCATGGAAGCTATTGTGGAGCAAGTTCGTGATGGAAGTAGTCTT CGTGTTTTTTTGCTTCCAGATTTTCAGTTCGTCCAAGTTTTTGTTGCTGG TATTCAGGCACCCTCTATGGGTAGAAGGGCTGTGCAGGACCCAATTCCTG AACCTGAAATGACCGCTGCAGCTGATGAAAATGGCAATGCTTCTACTAAA TCTCGTGGCCCATTAACATCGGCACAGAGACTTGCAGCATCACCAGCCTC GTCTAATGAAGTTACCCCAGAATTATTTGGGCGGGAAGGGAAACACTTTA CAGAGATTCGTGTATTACATAGAGATGTCCGCATTGTTCTGGAGGGTGTT GACAAATTCAGCAACTTGATCGGTTCCATATATTATCCTGATGGTGATGT TGCGAGGGATTTAGCTATGGAGCTTGTTGAACATGGCTTAGCTAAATATG TTGAATGGAGTGCTAGTATGTTGGATGATGAGGCCAAACGGAAACTAAAA AATGCTGAACTTGAAGCTAAAAAGAGTCGGTTAAGAATGTGGACAAGCTA TGTGCCTCCGGTTTCTAATTCGAAGGCAATTCACGACCAGAATTTTAGTG GAAAGGTTATTGAGGTCGTGAGTGGGGATTGCATTGTTGTAGCAGATGAT GCTCTTCCATTTGGCAGTCCATTAGCTGAGCGAAGAGTAAATCTCTCAAG TATAAGGTGTCCGAGACTAGGCAATCCTAGAAAAGATGAAAAAAGCCCCC CTTATGCTCGTGAAGCGAAGGATCTCTTGCGAACACGTCTAATCGGCCGT CAAGTACATGTTTCAATGGAATATTCTAGGAAGGTAAACTTGGCAGAGGG ACCTGGTGCTCCAACTGGAACAACAGATAGGGTAATGGACTTTGGATCAG TCTTTTTGCCGAAGGATGGCGAGGCTCCACCTAGTGCAGGCAGTCAGCCG ACAGGGATTAATATTGCTGAGTTAATTATTTCTCGCGGTTTTGGTGAAGT CATTAAACATCGTGATCTTGAGGTAAGATCAAATTACTATGATTCCCTTC TTGCTGCCGAATCGCGTGCCAAAGCTGGGAGGAAAGGGATGTATTCCGGC AAGGATGCTCCAGTGATGCACGTCAATGACTTTATAACGGCTTCATCTAA AAAAGCGAAAGATTTCTTGCCATTTTTGCAACGGAATAGAAGGATGACAG CTGTTGTGGAATATGTGTTTAGCGGTCACCGATTTAAATTATATATTCCA AAGGAAACTTGCAGCATCGCTTTCTCGATCTCAGGAGTGAGATGCCCAGG ACGAGATGAGCCTTATTCCAGTGAAGCGATTGCACTAATGAGAAGGAAAA TCATGCAGAGGGATGTTGAGATTGAAGTAGAAACTGTTGACAGAACAGGT ACTTTTTTGGGATCTTTGTGGGAGTCAAGATCCAATGTCGCATTGATACT ACTCGAAGCTGGTTTGGCCAGGCTTCAAACCTCTTTTGGTACAGATAGGA TACCTGATATCCACCTGCTTGTGCAGGCTGAGCAGTCTGCTAAGAAGCAG AAATTGAAGATTTGGGAGAACTATGTTGAAGGAGAAGAAGTTTCTAATGG CCCAACTTCTGAAAGAAAGAAAGAAGAATTTAAGGTAGTGGTTACTGAAG TCCTTGGCGGTGGAAAGTTTTATGTTCAGCCAGCAGATCAGAAAGTGGCA TCCATTCAGCAGCAGCTTGCTTCTTTGACACTCCAGGAGGCTCCTTTGAT CGGTGCTTTCAATCCAAAGAAAGGCGACCTTGTCCTTGCACAGTTTAGTG CGGATAATTCCTGGAACCGAGCAATGATTGTGAATGCCCCTCGAGGTGTC GTTGAATCTCCAAAAGACAAATTTGAAGTGTTTTACATTGACTATGGAAA TCAAGAAATGGTTTCTTTCAGCCAATTGCGACCTGTTGATTCTTCCGTGT CTTCGTCACCTGGCCTTGCGCAACTGTGCTGTCTTGCTTATGTAAAGGTT CCAAGTTTGGAGGAGGATTATGGGCAGGAAGCAGCTTTTAGTCTGAGCGA GCACACACTTAGTGGGCCAAAAGAGTTTAAGGCTGTTGTTGTAGAGAGGG ATGCGTCTGGCGGAAAAGTCAAAGGACAAGGAACTGGTACTGTCCTTATG GTAACTTTGATTGATGAGGAGGCTAATGAAAGCATAAATTCCCGCATGTT GAAGGAAGGACTTGCTAGGTTGGAGAAGCGGAGGAGATGGGAGCCGGTCG AAAGAAAACAGGTGATTGACGAATTAGAAAAAGACCAAGAAGAAGCTCGT ACTAAGAGGCTGGGCATGTGGGAGTATGGAGATATAATGTCAGATGACGA GGACAGTGCTCCAGTAAAGAAACCCAGTGGTGGCAGGAGTGCAGGCAGTC AGCCGACAGGGATTAATATTGCTGAGTTAATTATTTCTCGCGGTTTTGGT GAAGTCATTAAACATCGTGATCTTGAGGTAAGATCAAATTACTATGATTC CCTTCTTGCTGCCGAATCGCGTGCCAAAGCTGGGAGGAAAGGGATGTATT CCGGCAAGGATGCTCCAGTGATGCACGTCAATGACTTTATAACGGCTTCA TCTAAAAAAGCGAAAGATTTCTTGCCATTTTTGCAACGGAATAGAAGGAT GACAGCTGTTGTGGAATATGTGTTTAGCGGTCACCGATTTAAATTATATA TTCCAAAGGAAACTTGCAGCATCGCTTTCTCGATCTCAGGAGTGAGATGC CCAGGACGAGATGAGCCTTATTCCAGTGAAGCGATTGCACTAATGAGAAG GAAAATCATGCAGAGGGATGTTGAGATTGAAGTAGAAACTGTTGACAGAA CAGGTACTTTTTTGGGATCTTTGTGGGAGTCAAGATCCAATGTCGCATTG ATACTACTCGAAGCTGGTTTGGCCAGGCTTCAAACCTCTTTTGGTACAGA TAGGATACCTGATATCCACCTGCTTGTGCAGGCTGAGCAGTCTGCTAAGA AGCAGAAATTGAAGATTTGGGAGAACTATGTTGAAGGAGAAGAAGTTTCT AATGGCCCAACTTCTGAAAGAAAGAAAGAAGAATTTAAGGTAGTGGTTAC TGAAGTCCTTGGCGGTGGAAAGTTTTATGTTCAGCCAGCAGATCAGAAAG TGGCATCCATTCAGCAGCAGCTTGCTTCTTTGACACTCCAGGAGGCTCCT TTGATCGGTGCTTTCAATCCAAAGAAAGGCGACCTTGTCCTTGCACAGTT TAGTGCGGATAATTCCTGGAACCGAGCAATGATTGTGAATGCCCCTCGAG GTGTCGTTGAATCTCCAAAAGACAAATTTGAAGTGTTTTACATTGACTAT GGAAATCAAGAAATGGTTTCTTTCAGCCAATTGCGACCTGTTGATTCTTC CGTGTCTTCGTCACCTGGCCTTGCGCAACTGTGCTGTCTTGCTTATGTAA AGGTTCCAAGTTTGGAGGAGGATTATGGGCAGGAAGCAGCTTTTAGTCTG AGCGAGCACACACTTAGTGGGCCAAAAGAGTTTAAGGCTGTTGTTGTAGA GAGGGATGCGTCTGGCGGAAAAGTCAAAGGACAAGGAACTGGTACTGTCC TTATGGTAACTTTGATTGATGAGGAGGCTAATGAAAGCATAAATTCCCGC ATGTTGAAGGAAGGACTTGCTAGGTTGGAGAAGCGGAGGAGATGGGAGCC GGTCGAAAGAAAACAGGTGATTGACGAATTAGAAAAAGACCAAGAAGAAG CTCGTACTAAGAGGCTGGGCATGTGGGAGTATGGAGATATAATGTCAGAT GACGAGGACAGTGCTCCAGTAAAGAAACCCAGTGGTGGCAGGAGGTGA

Pep:

>DCAR_025630pep MATVGASGWLKGKVKAVPSGDSLVIMGNTKAEIPPEKTITLSSLVAPRLA RKNSDDEPFAWESREFLRKLCIGQEVIFKVDYEVPAIGREFGSVFIGDKN VACLVVASGWAKVREVREQSQQKAEVSPFVAELLRLEEQAKQQGVGRWSK VPGASEAAIRKLPPSAVGDPSNLDAMSLLAANKGRPMEAIVEQVRDGSSL RVFLLPDFQFVQVFVAGIQAPSMGRRAVQDPIPEPEMTAAADENGNASTK SRGPLTSAQRLAASPASSNEVTPELFGREGKHFTEIRVLHRDVRIVLEGV DKFSNLIGSIYYPDGDVARDLAMELVEHGLAKYVEWSASMLDDEAKRKLK NAELEAKKSRLRMWTSYVPPVSNSKAIHDQNFSGKVIEVVSGDCIVVADD ALPFGSPLAERRVNLSSIRCPRLGNPRKDEKSPPYAREAKDLLRTRLIGR QVHVSMEYSRKVNLAEGPGAPTGTTDRVMDFGSVFLPKDGEAPPSAGSQP TGINIAELIISRGFGEVIKHRDLEVRSNYYDSLLAAESRAKAGRKGMYSG KDAPVMHVNDFITASSKKAKDFLPFLQRNRRMTAVVEYVFSGHRFKLYIP KETCSIAFSISGVRCPGRDEPYSSEAIALMRRKIMQRDVEIEVETVDRTG TFLGSLWESRSNVALILLEAGLARLQTSFGTDRIPDIHLLVQAEQSAKKQ KLKIWENYVEGEEVSNGPTSERKKEEFKVVVTEVLGGGKFYVQPADQKVA SIQQQLASLTLQEAPLIGAFNPKKGDLVLAQFSADNSWNRAMIVNAPRGV VESPKDKFEVFYIDYGNQEMVSFSQLRPVDSSVSSSPGLAQLCCLAYVKV PSLEEDYGQEAAFSLSEHTLSGPKEFKAVVVERDASGGKVKGQGTGTVLM VTLIDEEANESINSRMLKEGLARLEKRRRWEPVERKQVIDELEKDQEEAR TKRLGMWEYGDIMSDDEDSAPVKKPSGGRSAGSQPTGINIAELIISRGFG EVIKHRDLEVRSNYYDSLLAAESRAKAGRKGMYSGKDAPVMHVNDFITAS SKKAKDFLPFLQRNRRMTAVVEYVFSGHRFKLYIPKETCSIAFSISGVRC PGRDEPYSSEAIALMRRKIMQRDVEIEVETVDRTGTFLGSLWESRSNVAL ILLEAGLARLQTSFGTDRIPDIHLLVQAEQSAKKQKLKIWENYVEGEEVS NGPTSERKKEEFKVVVTEVLGGGKFYVQPADQKVASIQQQLASLTLQEAP LIGAFNPKKGDLVLAQFSADNSWNRAMIVNAPRGVVESPKDKFEVFYIDY GNQEMVSFSQLRPVDSSVSSSPGLAQLCCLAYVKVPSLEEDYGQEAAFSL SEHTLSGPKEFKAVVVERDASGGKVKGQGTGTVLMVTLIDEEANESINSR MLKEGLARLEKRRRWEPVERKQVIDELEKDQEEARTKRLGMWEYGDIMSD DEDSAPVKKPSGGRR*