DCAR_027406 | CarrotOmics

Resource Type:

Gene

Organism:

Daucus carota subspecies sativus

Name:

DCAR_027406

Identifier:

DCAR_027406.gene

Locus Tag:

DCAR_027406

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 2 relationships.
Relationships
The mRNA, DCAR_027406, is a part of gene, DCAR_027406.
The genetic_marker, MK004688 K2132, is located in gene, DCAR_027406.

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcripts:


Transcript Name	Identifier	Type	Location
DCAR_027406	DCAR_027406.mRNA	mRNA	DCARv2_Chr8:23414416..23422252

Sequence:

ATGGACGCCATTGGTCTCTTGAGAGCTCTTAAAATTAAACCATCCATTTT
GAAGCCACCCAACAATCCTCTTCTGGGTGTTCGCAATTGTTGTAATTCAT
CATCATCTGCAAGCGCAATCCCCACGGGTCAGTTTATTCAATAAAGTTCA
AGTTTTTATATGATTGTATTGAGCTAAGCTGAATCCTAATTGTAGGGGTT
AGCACAGTGAAAAGAAAGTATGATGCATTGTTGTTGGATGCTGGAGGTAC
CCTTTTGCAGTTGGCTCATCCTGTTCATGATATTTACTCTTCGATTGGTG
TCAAATATGGTTAGCTCTTTCTTTCTTTTTCTTGGTTTTTGAAATGTAGT
CCATTTTTTCGTATCCGATATTATGCTAAAGAAGATATATGATCCGAATA
CGCTTCTCTGCTGTTTGATATGTGGGGCTAATCTATCCAGAGTTGGTAAT
TGGACATGGTCTGTGGTTTGGTGCCAGAGGTCTCGAGTTTTAACTCCGTA
TTCACTCAAATTCTATGGTGATCCCCTGAACAATATTGAATAGGTTTTTT
GGTTCGAGTGAGTGTAAGTTTAATGATATATGATCCAAGTAAACCTCTAT
TATCCCCTTTTATGGTTTTGAGAGAGGGTAATTGTCAAATTAAGTTGTTT
GTTTTTTTGAATCGTTGCATGAAGATTGTTAATCGACAAGAATTTAATTT
TAGTGACAATCGTTAGGACTAAAATGATTGTTCCATTTTTAAAATTATTA
ATGGGAAAATTTCTATATTTTATTTCTTTTATTAAAAAAGTAGTCTTATT
TCAGTTTATGGCTGATTAGGGAGGGTATAAATTTATAGATATTCAGTTTT
GATTTTAGTCTGAGAATTCGGAGCAGTTAATTTCAGTGGCATTGACAGGT
CTGAGTATGTCCCCGGCCGAAATAAAGCAAGGATTTAAAAGAGCGTTTTC
CGCACCATGGCCTGAAAAACTTCGATACCAGGTGAAATGATTTCTCTTTT
CTATTTTTTGTTTAGCAAAACTGCAAAAGGCTCTTGCTCCATGTCAATAC
CCTCAGCCCATGTATGTGGAGCTTGAGGGTTCAGGCCATGTCAAAAGCAA
AGTGGGTAAGTGTCGTCGTAGTCACGGAGGTAAGTCAAGTTGAGTCAATG
GGGACCATGCTTGGCGACTAGTTGACGAATTAATTATAAATAATTCATAT
ATAGACTATATAATAGATATTATATATAATTCTGACATTTACCACTTTAT
TGTAACAATAAACAAACTGAACAAGTAAAGGTTCACAATGTGAATCTAAG
AAACAGTGGTGAGTGTTCATTCAATCTGCCAAAAAATCAAGAAATAATTT
TCTTAATCTCAAAAAAGGGAAGATCAACTTTTCTCTTTTGTTTGATGTAA
TATTGTATGTTTTTTTATTTGTAATTATTTAGTATTGTATGCTGTAATGC
TCTTTATTGATTAAAGTACTTAACTGGATTTACATCGTGCATTCTAATAT
ACATCATACATGTCATGATTCATCTAAACATTCTTGCAGTAAAGAGGTAG
CATTATCAGACACGGCCTAAAACTTGAGCCGATTCCTAACTCGATTTACC
GAGACAAAACCCGAAAGTGATCTGAAATAAGAATTTCATTGCCTTTCAAA
TATCCAATTTCATGCAGTTTTAATAATTTTAGCTTGACCCAAAATTGACC
CGTTTACAGACACGAACCCCCAAAGGCCTGGTCGTTATTTAGATGTATAT
TCTCCTAGTAAAAAATGTGCTAAAAATTAACTCGAGCTGCTGATATGTGA
AATCTGTATTGTAACAAGGCCTAGGAATATATTCATCTATGGATAAGTGT
GTCAAAGAAGAAATGTGATTCACTAGTCCTATTTCCTTCATTTGTTTATG
AAGAAAGGAGACAGTAGCTTAACAGTCAGTTGCACCTTTTGATCAAACTG
GTGTCATGCCTTAGAACTAATTTAAGAATGCATAATATTGAAATTATTTA
ATGTTTTAACCACAGAGATATGGCAAGAAATATGTAGATATATTATGTCT
TATCTATATCGATGTTTAATAACAGGGAGATGGAAGGCCATTCTGGAGGC
TTGTAGTTTCTGAAGCCACGGGTTGTGCAAGAGACGATTATTTTGAAGAA
GTTTACCAGGTAATCAAGCATAATAAATTTTATTTGCACTTTAGGCGAAG
GTGTTGAGTTTGTCCAGTTCTTCCAATCTTGTATTATTAATTATATATAA
ACACACTTGTGCACGAATTGACCATGAAGTATTCTAGGATATCATCACGG
CTGGTCTCCTGTTATAACTAATGCTGGAGTTTCATATATTTGATGGGTTT
TTTTTTCAGTACTATGCAAATGGTGAAGCATGGTGTCTTCCACCCGGAGC
CCATGAAACAATAGTCATCCTGAAAGATGCTGGAGGTTATATTTATGAAA
TACTCTCTTTATCTGTTGCAAATTATATCTAGAAAACTTGATGTTTGCAA
ATTATGTACTGACCAGTTAATATTTCTCACCATGGCACAGTTAAATTAGC
TGTTGTATCCAACTTTGATAATCGTCTGAGGAAGCTATTAAAGGATTTGA
ATGTTGTGGACCTGTAAGCATTGATAACCTTACTTGTCCAGCCTCTTACT
GCTGCTGTAAACTTACTAATCTTAACACCTTTGATAGATTATACTGTTCC
TAATCATAGGGAGCCTTATATCATCCATGAAATTTGTCATTTAGCCAATA
AGACGCAGTTTGGTTGATTAGTTACTCTTCCAATGTGAAGCAAACCAAAA
CATACCCCACGCGGGAGAAGGATCTTAGGAGGGTTAGTTTTGGGCAGACC
TTATCCCTACTCTTGACAGTAGAGAGGTTTTTTTATGTGAAAACCCCCAG
TTTTAAAATAACTAAGCAAAAAGAAAACAACATACAACACTAAAAGTGTG
CAAAGTACTAACGAATAGAAGTAAGTGTGTTAAAAGTCAGCATAAAAGAA
ACAAATAAGACGATAAACGAACTATATGTTTGGAAAGTACATACTAATGA
CCACAAAATACTCCTAAATACTAACTGCAGGGCTTACCCAGCTCACCCTA
TTCTTCAACTTCAAAACTATGGCGACTGTCACAGCCTATGTGAATCTGCT
ACCTTGATTTGGGTCTCAGCGGTTTCTTATTTTAATTCATGCCTTCGGGG
AGCTTTGCAATATTCCATGTCCAGTCCCAGCTCCTCGCGTCCTCCGTGTC
CTTACTCTCCTCCCTATCCCTCAACCTAAACACACTCCACCTTTTTTGAG
TCATTATCCAAAAAGGAGAATAGTTAATTACTAACAGTAGAATGTCTAAT
AGTATACAAAGATTGCAGTATTTTGTGGCCTTCTCAAACAGGGTTATTGG
TCTTAATGGTCAGTACTAATTTTATACGACCCTTTTATAGGTTTGATGCT
GTTATTATATCTTCGGAGGTTGGATATGAAAAGCCAGATGCAATGATATT
TAAAGCCGCTTTAGGTATATGTATGGAATCACAATTTTTTTTCTTAATTT
CAGGATTATGAAGGGTGTTTAAATTGTCAACAATATGAGAGAATCTTATA
TATTGCCTCATTAGCCCTCACATATGTATCTGTACTTGAAATTTCTGAAA
GGGGTTACATAAGCGAAAAAGCTATCTAACGTTTAGCAATATTTAGACCA
GGTAAATGTGGAAGCCTGTAGAGCAGTACATGTTGGAGATGATGAGAAGG
CTGACAAGTTGGGAGCCAGTGCTGTGGGAATCAACTGCTGGTAAGTTTAG
TCTAATTTCCCAAGACACCTCCTAATTCTCATATCTACGCAAGTTCTATT
ATTACATTGTGGGGAAGAAATTTTAAATAACTTGTCACTTGTATAAGCAA
GTTCTGAGGTACATAAATATAATAATTTTCAGAATATTGTTTATGTTCCA
CTGATTCATGCAGGTTATGGGGAACAGATGTAAAATCCTTTCAAGACTTG
CAAAATCGCATTCTCCTTCCACAGTAATAACTCTGCAAAGGGCCTACGTT
TCATCGAGTGCTGATTGAAACTGGCTTCAGGCTCTTGCATTGTCAGTTAT
AAATATCGGGCCTCATATTAGTGCTCCTACTTCTAAAGTGGACTTACTCC
TGCAACTTATGATGCAGCCTGTGGGTGGAGTCTGTGGAGAGCACTCTATA
ATAAGAAACAGAAAAGAGCCAGATAATTGCTGCTTTTGGTTGAGTGTTAG
TGCAGCTAAATGTAACTAATATTTTATAGCCAATGTAACTAATTTTTTCA
TTTACCCTCTTTTGTGCTGCAGAAATCACTCAAAAACACAAATTAAGCAG
ACAATAAATATAACAATAATAAGCACTAAATTAAAATCTCTAACGACTAC
AAAAGTAATACAGTAATCACTATTGGTGATCGAAATAAATCAGAGTCAAG
AAATAGATGCTTACTAATAAACACATTTTACGATTTTTGATATACAGACC
TACAGTTCTTAAATTACAAATTTATTCTTTGAATTATTTAAAAAAACTGA
CCTAAAAATTGAAGTTAAAATACAGTCTCCGTTTGAAAATAATTGTCAAG
GATTTTTATTTTTTTTGTGTGATCAAATTAATCCAATCAAATTTTGTTTT
AAATTTACTTTCACATAATTTATATATCTTAAATTAGATTATATATATTT
TAATAAAATCATTTTTATTCATTTCAGTATAGATAACATATGTAAATTGT
GGAAAATAATTTTGAAAAGGACGAAGTAATTAGTCGAAGAAAATTAGTTT
GTCAGTAGCTTTGAACAAACGCATATTGTGCTTCTCAAGTAAGCAATGGT
TGCTCTGATGGATACATATACATGTAAATTGCTAGTTTTGGTTTTAAGTT
TGCAGTGAAAATGGCAGAACAATCATCAAACCGGAGTTTGTTTGGTGGCG
CTGTTGTCTGTAATTTCCCTCCCCGTTTTCAGGTCCCTCCCTCCCTCTCT
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTGTTTTT
TTTTCTCTCATCTTCTCTCTCTCTCCCTCTCCCCCTTCTCTCCCTCTCCC
TCTCTCTCTCTCTCTCTGTTTATGTGTTTGTTTTATTGACTTCAAAAATT
ATGATGTTGTGAATTGTTGTAGGATGTGAGTGATGTTCGTGAAGTTCCTG
ATCATCAGGTTTGTAAATTATGCTTTCCCTTTTTGATTATTTATTTTAAC
TTTTTAAACTACAATTACATGCCAAAGCGGAGTTATATATGCCTTGTTCT
TGTGAAGACATTTGTCAAGCACTCGATAGCATTTGCAGAATAATGTGTAT
AAAATTAGAGGTGCTCAAACATTAACTGTTTATTGTGAAGTGGAATTTAA
TTTGTTAAATTCTTTGTAGGAGGCATTTGCGGACCCTGAAAGAGATGAAA
GTTTGATATTTGAGCTGTTGGATTTGAAACAAGATGTTCCCGACAATGGA
AGTGCTACCTGGTTCTTTCAAGACCTTGCCTCTGAACAGGACTCTGGCGG
AAACATGGTACTTCTTCACTCTTTTTAAATGTTACTCCATATGTTACTTA
TTGTGCAAGCACGGACATTTAATTAATTTTATGTATTAAAAAACTTGTTG
GTTTTCTAGTTATTGATGGTGTACATATGTGCTGTGTGCATAATCACTTA
ATCAGAATGCCCTTCAGCACAATTTTCGTGATATAATGATTTCTCTAGGG
TAGTATATAAATATTAAAGTCTACTACTTTATATCAATACTCCCTCCGTC
CCAGTCATTTCTATATAGTTTGGGATGTCCCATCATTTCTATACATTCCT
AAAATAGCAACTTTTAATAATGTAAAACACTATTATACCAACTACTTTCT
TCCACTATCTTCATTTTATAATAATAAAAACACTATTACACCCACTACTT
TCCTCCACCATCACAAATCTATAATTAAATACAAATGGGTCCCGCCACTT
TACCCGCTTTATCTATTTTTCATCTAACTTTACTCATTTTTTACACTTTT
TCTTGGTCTCCGTGTCTACACCAAATGTATATAACCTATTGGGACGGAGG
GAGTATTTATCTTCCTTGCTACTTTTCTTGTCAATATTGCTTGCCTTTTT
ATCCTAATCAAATAATTGCTGATTACCGGATCAAGAAATAAGTGTTTAAG
ACCTGAAGTTCTTTCTTGCGGAGGAGCTTGTTGGCATTATATAGTACTAT
TACTACTAGTAAATATATATGCATGTGATTACTATGCTTTAATTGCTATT
TTATTATGTCTTGTACTGAATCAGATAACAATTATACATGATTTCGAATA
TTGTTGCCACCAGGACATGTCATCTGCTCTTTTACCAATGCTAGATTGGT
AGTAATTGGGGTAATGGTAGAGCAATAACAATGGTGGCTTCCTGATTGTC
TTTTTACCCTTTGTTCACTTGCATTTTTCAGATAATTGAGCAATCAGGAG
TATTTGAGGCCGAGTGGTTGCGTTTTAGAAACATGCCTGCAGTCATGACT
ACTGCCATTGGACAAATGGTATAACAGCTTCACTAATTGTTGCTAGACTT
CTACACCCTTTCATTTCGACTTTCTTGTGTTTTATTTGATTCATAACTAT
AAACAAATTACTTTGGCTATTGATGCTTACCCTGGCGCAAGTCTGTGTTG
GTAATCCGTCTGCACAATCCACCAGTTGTTCATGCTGTTGACTGTATCTG
ACTCACTAAAGTTACTCGCATTTTATAGAAGTCATAGTTCTACTTGACAC
ACTAATTTTACTTTTTACTAGGTTTTGCGATTCCATTAGTGGCTTTTCAA
TTAATTAAGCGGTTTTATGGTCAGCATCGTTGCATGCTCAATTTATTGTA
ATGATTCATTTACCTGAATTCCCATGCTAAACTTCTGTTTCTTTCATTTC
CATCTGCATCATAAAGTCTCCTAATGAAGTTGAGTCAGGCTAGAGATAGT
GATGTATAGTTTAAGAGGACAGAACACAGTGGTTTTTGCTGAGAATGTAT
GACTGTGATTCAGTATGATTTACCTTCTTCATGTCTGGATGACTGGATAG
TGGTACTAGATTTTTGTAAAGGTGACGGATACCCAAGAGTCTAATTTATT
GAAAATTTATTCAGGCACAAGTAGCTGGTATGGTATTGTTATATGTTAAG
TCAAGTGAATTGATCAATTTATTAAAGCTTAAATTTTTTTAATACGACTG
TTTGTTACTTAAATGTATGAGAATCGATTGGATTCTAAGAGAAATAATCT
GGGTACTGGTTGGCCAAAAGGAATAAGAAGAATAGATGTTTGGCAATTGG
GAGGATTCAAGATATTAGTTTCCTTTAGTTTAAGTAAGATGGTAAATGAA
GGAATTATATTTGACGTGCTTTGCTGTAAAATAAAGTAATAGCATCAACC
ATAAACAAAAGCATTAGTGGTATATGTCGGTTAGCAAACCGAAGCGATTA
CTCGGACATGATATCAATGCAGAAAATAGCACATCCTTTCAGCCATGCAT
ATCACTATGCATTCCCTTGCCCCAATGTGCTTTTATGCATAATACAATGA
CTGCCTGTTCTACTTTTCTGCAGGCCATCTCTAAGGGCCGCCAGGGAAGG
GAAGCTCAAAATGTTGTAAAGGCAAGTTGTTTGCCAATAATGTAAATTTC
TTATAGTTAAATGCAGTTGAGCTGACTGGCAAGTCTGAGAATTTGGTTCC
TGCAGGTGTACCTTGCAAATTTGCGCCTAAAGGAAGTCGGCACAGATGTA
CTTATCACTGCCTATGAGCCCCTTTTGATAAAGTAA

Sequence Length:

7836

Sequence Checksum:

ce623c660be1175dd2214b273809c755

View location in JBrowse:

DCARv2_Chr8:23414417..23422252+

Cds:

>DCAR_027406cds ATGGACGCCATTGGTCTCTTGAGAGCTCTTAAAATTAAACCATCCATTTT GAAGCCACCCAACAATCCTCTTCTGGGTGTTCGCAATTGTTGTAATTCAT CATCATCTGCAAGCGCAATCCCCACGGGGGTTAGCACAGTGAAAAGAAAG TATGATGCATTGTTGTTGGATGCTGGAGGTACCCTTTTGCAGTTGGCTCA TCCTGTTCATGATATTTACTCTTCGATTGGTGTCAAATATGGTCTGAGTA TGTCCCCGGCCGAAATAAAGCAAGGATTTAAAAGAGCGTTTTCCGCACCA TGGCCTGAAAAACTTCGATACCAGCAAAACTGCAAAAGGCTCTTGCTCCA TGTCAATACCCTCAGCCCATGTATGTGGAGCTTGAGGGTTCAGGCCATGT CAAAAGCAAAGTGGGTAAGTGTCGTCGTAGTCACGGAGGGAGATGGAAGG CCATTCTGGAGGCTTGTAGTTTCTGAAGCCACGGGTTGTGCAAGAGACGA TTATTTTGAAGAAGTTTACCAGTACTATGCAAATGGTGAAGCATGGTGTC TTCCACCCGGAGCCCATGAAACAATAGTCATCCTGAAAGATGCTGGAGTT AAATTAGCTGTTGTATCCAACTTTGATAATCGTCTGAGGAAGCTATTAAA GGATTTGAATGTTGTGGACCTGTTTGATGCTGTTATTATATCTTCGGAGG TTGGATATGAAAAGCCAGATGCAATGATATTTAAAGCCGCTTTAGACCAG GTAAATGTGGAAGCCTGTAGAGCAGTACATGTTGGAGATGATGAGAAGGC TGACAAGTTGGGAGCCAGTGCTGTGGGAATCAACTGCTGGTTATGGGGAA CAGATGCTCTTGCATTGTCAGTTATAAATATCGGGCCTCATATTAGTGCT CCTACTTCTAAAGTGGACTTACTCCTGCAACTTATGATGCAGCCTGATGT GAGTGATGTTCGTGAAGTTCCTGATCATCAGGAGGCATTTGCGGACCCTG AAAGAGATGAAAGTTTGATATTTGAGCTGTTGGATTTGAAACAAGATGTT CCCGACAATGGAAGTGCTACCTGGTTCTTTCAAGACCTTGCCTCTGAACA GGACTCTGGCGGAAACATGATAATTGAGCAATCAGGAGTATTTGAGGCCG AGTGGTTGCGTTTTAGAAACATGCCTGCAGTCATGACTACTGCCATTGGA CAAATGGCCATCTCTAAGGGCCGCCAGGGAAGGGAAGCTCAAAATGTTGT GTACCTTGCAAATTTGCGCCTAAAGGAAGTCGGCACAGATGTACTTATCA CTGCCTATGAGCCCCTTTTGATAAAGTAA

Pep:

>DCAR_027406pep MDAIGLLRALKIKPSILKPPNNPLLGVRNCCNSSSSASAIPTGVSTVKRK YDALLLDAGGTLLQLAHPVHDIYSSIGVKYGLSMSPAEIKQGFKRAFSAP WPEKLRYQQNCKRLLLHVNTLSPCMWSLRVQAMSKAKWVSVVVVTEGDGR PFWRLVVSEATGCARDDYFEEVYQYYANGEAWCLPPGAHETIVILKDAGV KLAVVSNFDNRLRKLLKDLNVVDLFDAVIISSEVGYEKPDAMIFKAALDQ VNVEACRAVHVGDDEKADKLGASAVGINCWLWGTDALALSVINIGPHISA PTSKVDLLLQLMMQPDVSDVREVPDHQEAFADPERDESLIFELLDLKQDV PDNGSATWFFQDLASEQDSGGNMIIEQSGVFEAEWLRFRNMPAVMTTAIG QMAISKGRQGREAQNVVYLANLRLKEVGTDVLITAYEPLLIK*