| Relationships |
|---|
| The mRNA, DCAR_029930, is a part of gene, DCAR_029930. |
| The genetic_marker, MK011475 9_17878533, is located in gene, DCAR_029930. |
| The genetic_marker, MK005110 K2554, is located in gene, DCAR_029930. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_029930 | DCAR_029930.mRNA | mRNA | DCARv2_Chr9:17872377..17878739 |
ATTCATTAATATTTTTTAAAAATTTAATAATTGAATAACAAAAAATTGAT
TAAATTCTTTAAAAAAGGTAATTGATATAAAATTTTAGTTGTGATATTTG
GTTAAAATCAAATTAGCGACAACGATTTGAGATACAACTCAACTCGGTTA
TTTTAACAAACGATATATTTTTGATCTCGCAGCACCCAAAACCCTAAGAT
TACAATTTACAACCCAGTAGAACAATAAGCTGCAAAACCCTCCCTTTCTC
CCAAAACACACACACACACACACACTTGCCGTAGACTCGAGATGTTGAGA
AGACAAGTGAGTTCACTCATCTCAATCTGCAAACAAAATGGATCCACGTA
TCTCCAGAAGCCCATTGTCTCAACAAGTATGATTCCCCAACACCCACTTC
AATTCTCGAAATGGGGAAACTCAAAGATTGGATTTTTAGGCACAAGGGCA
TATAGTCTGCTGAGTTTGAATGATTTGAGGGATAATAAAGGTGCAAGAAA
ACCAAAGACTAGAAAGGGTCGTGGGATTGGGTCTGGCAAGGGCAAAACTG
CAGGTAGAGGTCATAAGGGTCAGAAAGCTAGGGGGACTCATAAGTTTGGC
TTTGAAGGTGGTCAGACTCCGCTTCGTCGCAGACTCCCCAAAAGGGGATT
TCATAACCCCTTTAGCCTCACTTTTCAGGTATCTTGTCATCTCTCTCATT
CGAATGTTACAATTTAATATAGTAATAATAATCCTGTGATATGCGAACAA
TGCTTGCAAAGATACGACATAACAAATACACAATGGAATCAACTAATCAA
GCCAATCACTGGATTGACATGGTCAAGAAATAGACATAATGATATAGCAA
TGACATTTTAACTGACACTTCAGAGAAAAGCTAGTTGAACTCAAACATAA
AAATTTAAGCACAAAATGAGGCCTTGCATTTAGAAACTTTGAAAAGTTTG
GTAACTACATTTCTGCATTGTATCTTTAATGTGAGCCGTGATGAATCTAA
TTGCTTCTCGAATCTCTAAAGATCGCTTGGAAACTCCAAAGCTATGTTCA
GAGGTTTGTCTGATCTTCCTTTCTCCAGTAAAAGTATAGAGTGTGTTCTT
AAATTTATGGTCTAACTACAATCATTAAGCCTCTTTTAGCCTTTACCGTT
ACTTCTAGAGTTTCTTTTTTAGTCCTGTAGCCTGAAAGAGATTCTTTAAT
GATTCTTCCTTGGTGCTTGTGTAAACTTGTGCTTATCCTAGAATGAAAGA
TATTATAGTATCATGATGTTTGGGTCACTTTTTGTGGGATGTTAAAACTT
GACTAGTGTTGCATGATATTCTTGTATGTTTCTGTGTACTTTGACCATTA
GTTTTAATATTCATCTTTCTTTGTGTCGTATGAATTGCTATGATCACGGC
AGCCTGTGGGGTTAGGAAAGATTGCAAAGCTAATAAATGCAGGGAGGATA
GACTCTTCAGAATTAATCACCATGAAAACTCTCAAGGTAATTGCGATTTT
TTCTATGATTTCTAGGTTCAGATTTGTACTAGTGTTGCCTTGGCTGACAG
TCAGATAGTGGGCGCCATAATATTGTAGGAAGCTGGTGCAATTGGAAAGC
AAATACGTGATGGGGTTAGGTTGATGGGACGAGGTGCTGAACATATCGAG
TGGCCTATTCATCTTGAGGTGATTTTTAATTAATACTTCTTACAGATCCC
TTTTGTGGCAGCAACAATATATTCTTCTTCTTCTTCTTCTTCTTTTGGTG
CTGAACATATCGAGTGGCCTATACTTATATTTATGTTTATTCTCTCTTTA
TTTCCTTTCTCTTACTGTAGTAAGTATGGTTTGTTACTTCGTTTTACTTA
TTCTTTTGGTGTTGCATTTATATATTTTGGTCACTCTCCTGAGTACACTG
TTCTGAATACAAACTAGGTGTATGCAAAAGAAATCCAGAAAAATGAAAAG
TGCTCAGGGAAAACTAAAACTGAGAGAAACAGAAACAAAAGGTTTAGTTA
CAGTTTAGGTCATATCCATACTAGAAAGGTTCGAAACTATGCTTTTGCTT
ATGTATAGTCATGGCTAAATGTGTTCTCCTTTATTGTATCTTGTATTATT
TGTAGAGTGGAGGGAGTAGGTACAGTCAAAATCTTTAGAAGCAACAAAGT
GAATGAAATTTAACCATTAGGAACACAAACAAATTAGTTTAGCTATAGAA
TAGTTATAGAAGTACCCTTAACTGCATACTTAGCCCAAACCACATCGAAA
TTAGCTTTTGTACTTCGCTTGAAATAGTTGAAGTGCCTTCTATAAAGCAG
AGAAACCAGACAACACTTATTTGCCAAGGGAAGGCACAAAACAGATTTGA
AGAGCAGTCTTGACCAATTTTGACATAAATATCACTTTGGCTGTCATTTA
TTTATTTAAGATTATTAACAGAATCCCTAGGAGGTTAATGACTTTATTCT
ACTGGCCTTGGCAACATGATAATGCTATTGAGAATATTTTCACATCATTG
ATCGTGAATTTTCCATGAGGCAATTGTTCGGCTCTTCCTCTACCAAATTT
AAACAATCACTTAATCTGGTATAAACAATAAAATTTACAAGTTAATTACG
ACAACTGACTCCATTTAATTTCAAAAGCTTCATCCATGCTCTTACCTATC
ATTCTACTCTGTTTTATCAACATCTTAGCATTGCCTTCCTTGAAAGTTAA
AAGTAATGTTTATGCGTATTGAAGGTATGAAGGTTCATAGAGGTGACTTT
AGGGAATTTCTTGAAGTCATTGTAACTAAAGTAACCTTTTTTATGATTTT
TGTGCTAAATAAAAATTATATTAGAATTAAGGAATGTACAAGATAGAATG
TGCAACCCTTCAATGTTGAATACATCAATGCAACACAGTAAATCTAACAC
TAGATTCTAAGTATCAGTAACAGAGTTAACTGTACTACAAACTAAGTCAA
TAAGCAAAAAAGAGGGGGTTCCCCTTGAGCTGCCCTTTGAAATCTTGGAC
AGGATAACACTCTCACTCGCACAAGGACTGCACTTTTCTTAATCACTGCT
GCACTGTTGCTAACTCCAGGGTTGTGAATACGTCTGTTTCTTTCAGCCCA
AAGACAATTAACAATTACTCCTAAGCAGTGTTGTGAAAAGCGCGCCTAGG
CGAGCGCCTAGGCGCAAAGCGAAGCAAAGCGCACCCTTAGCGCCTCATAT
ATGTCGAGGCGAAGGAACTTAAATGAAGCGCACGCTTTTAGCCTTGTAGC
ACAAAGCGCGCTTAAGCACGAAGCGGTAGAAAAGCGCGCTTTAGCGAAGC
TAAGCGCATAGTAAGATATTTAATGTTATTGGGCCACTTTTAAGGCCCAA
AAGTACTAACCCATAACAAAAAAAAGAATTTTGGGTCACTTTTAAGGCCC
AAAAGTACCAACCCATAACAAAAAAAAAGAATTTTAGGCCCAAATAAAAA
GTACTAGCCTGGAATTCTGCCTATGAAACATAAATTTTGAATTTTAATAT
TTAATTTTAGGATTGTTTTTCCCTCTAAAAGTATTATTTAATATATTATA
AAGGATACATTAAGTTATTTTTGGCTTAAAGTGTGATTTAATATATTATG
AAGTATAAATTATGTTATGAACCTATATACATATATCCTAAATAATAAAT
AAAAAAAATTGTAAAACATGCGCCTAGCTTCAAAGAAGCGCGCGCCTCGC
TTTTGCGCTTTGCGCCTAGGCTCCAGAGAGTTTTGCGCCTCGATGCGCTT
AATGCTTTCGATAACACTGCTCCTAAGTAAAGAAAATTAATCTGGCTCTC
GAGTGAGTGAACTGCACTAAATGTGTGCGCAGTCCAGTTGTGAGAAATAT
CAATAGAGCATCTTTGCGAAATGTCCAGGGTGAATGTACAGTCCATGAGA
AAATGATTTGGAGTTAGAGTGTTGTTGGCGCGTAATAGGTGGGTCTGGCC
TGTTTGCATTTAAAAAATTAACATTCTGTCTTCCATCAACAACCTCTTTT
TTAATGGTAACCAAAAGGTGAAGGAGCACCAGGGTATAGGCATAATATGC
CAGACCCAGGAGTGCCAATGCACCTGTGGCCTGGTTACGAATGGTGTTCC
ATATCAGACTAGTCGAGGACTATTGACCTGAGATTTGTCATGTTAAAAGT
CCACTCACCATTACATATAATGGAGCTGACTTTGGCCTGGAAGTAGGATT
TCATAATGGAGATAATTGAATAATGAAATCTGTCTAGAAAGGGAGTCTTA
GAAGCCCAAGGATCATGCCGAAGCAGGGTCTGGTTCCCAGAGCAGACCCG
AGCAAAGATGCGCCTCAGAGCCAGAGGTCTGTGACGTCTGTCGTTGAATA
TTTTTCTAATTTCCAGGAACATTTTCTTGGTTTGTTCCTAGTCCAAAATT
TCTTTCACCTGAGCACTGTTTCCATATTGAGCTTTGCTTATTCGTGACAA
TATGCACTAGATGCTTTAACATAGCTGCCAGATGTCACTCAAAATTTTTT
AAATGCCAAGTCCTAATTTAGGCAAGCAACACTCAACCCAAGAGACTTTA
TAATGACATTTCCCAACTTCCCCTCCCAAGGAATTTTTCAAGAACAGATT
GGGTAGATTTAAGCATATACCTACAGGCATAAATAAATTGTCTATCCAGT
ACATGATGCTACCATATAACACTGCTTTTCTTAATCAGTTGGAGATGACC
TGCAAAATTCAAGTAATTGCTAGTCCAGGTTCAAATTTGGTCGCATATTC
TTGCTACTAAAGGACTACAGTCTTGTCTGGTTAACTTGGTGGGGAAAAGA
GGTCACCCAAGTATCTTGTAGGCAATTCACCCCAGCTGATTTTCGTAGTG
GCAAGAATTTGCTGGATTTGGTCCAACGGCATGCTACTAAAGAAGCAGGT
GCATTTATTTGGTTTTGGACAAAGTCCAGAAATCTTGGAGAAATGATCAA
CTCCATTCATTATAGCTAGTACAAAATCGACATCTCCATAAGAGAACAGC
AGAATATCATCTGCAAACACCAAGGGGGTTAAATTAATGGCAGCTGCTCT
TGTATAGAATTTGAACTCAGGCCTCACCGAAGCTTTTTGCAGCATTGAAT
TAAGAACTTAATGGCAGCTGCTCTTGTATAGAATTTGAACTCAGGCCTCA
CCGAAGCTTTTTGCAGCATTGAATTAAGAACTTCCATGGCTAACCAAATA
GCTTGGGCAAGAGGGGCCCGTCCTGTTGAAGCCCAGATCTGCCTTGAAAT
TAACCCTCAAGCGAGAACATTGGTGTCCTGAAGCACTTTTTTAACCAGAT
TAGAGAACTGAACATAACATCGAGGGATCCCTTCTTTTTTGCTAGCTCCG
TTGCGCTTGGCTGGACATAGCATGAATCTGATGAATCTTGTTGGTACTTG
CCATAAATATTTATAATTTTTTGCATATACTACCTCTCTTTGATGAAATT
CACGCTTTGCTTCGTGCTTAAACTACCTCATTTCGGTCGCTAACGTTTTA
AACCCGATTGACTTTGTTGGTCTCTGTATCGTCCATTTCAACTCTTATGA
GCTTTTTCTCGTCAAATTTGTAACAATTAGATAGCATTTTTGTGCTAATT
AATACTCCCTCCGTCCTACTCATTTTAAGGTGTATATAAAATATATTTTC
ATAACTTATATTTGGAAAACTTCCATTCTTGAAGAAAAATTTAAACTTTA
TATTTATATTCAGAAGGATTTTTTTTTTGAAACTATATTTTATAGATTTC
TTGAAATGCGTGTCAAGCTCCCCATCCCAAATGTAAAGAACCTGAAGGGA
CAGGGGGAGTAGGTTGTTTTATAACTTGTTACTCGAATGAGTAATTTTGC
GTTTAACTCTGATTAGCATTCCCTCATCTATTAATATTGTATAACAGTTT
CGTTAGCATTTTATGCTAATTAATATGTAGTTTTATCTCTAAACTACTGC
TGTAAAAAAAGTTTTCCTTATTACTCTAGTGAGTACTTTGCATATTTCTA
ATTCGTTAGTAGTAAGTTAGTAACAAGTTTTTACTACTAACTAATTAGTC
TTTGTTTGTGTGTGTAAACAATAATCATATGCGATTAATGCTTTATGGTT
GGACCCCCCTGTATAATGGTAAGTCCATCAGATGTTTATTTTGTTACTTA
GCTTGTTAAAGTCTTTATATAGCATTAGCATTACTCAAAACAGAAATTAA
AACTGTATGCGGTAAACCTAAATTGAATAGTTGTCGTGTTGATATCTTTT
CTCATGGTTAGTAATTGAATATGTCAGTTTTGCAGTAAACTAGTGAGATC
AGATAAAGTTTGTTTAAAAAGCTTACTTTCGGAACAAAGCATGTAAAGTT
TTACTAATGACATAAAATTTCTGAATTATACTGTTGCAGGTTAGTAGAGT
GACAGTAAGGGCAAAGGCAGCTGTTGAAGCAGCAGGTGGATCTGTGAGGA
GAGTTTATTACAACAAATTAGGTTTCCGGGCTCTCCTTAAGCCGGAGTGG
TTTGAAAAGAAGGGAAGATTGCTTCCAAGAGCAGCAAGACCTCCACCTAA
GCAGGCAGATAAAGTGGATAGCATAGGACGTCTTCCTGCCCCAACAAAGC
CAATTCCTTTTACAGCCGAGGAGAAAGAGGCAATGTCCTCCCCAGCCATT
TAAGGTTTTTTTCTTTTTGCTATTCTTGGATAAGTTATGAAACAACAGGT
AATGTCTTTTTTGTTATTGCCATCTCTCTTCAGACATAAAATTGACAAGG
GAGGATTTGATGGATTTTTCTCTTTCCTGATCGTGTTCAGAAGGAAATCT
CAATATCACGTATGGCCTAAAGGTTCAAGGCTGTGAAATCATG