| Relationships |
|---|
| The mRNA, DCAR_009323, is a part of gene, DCAR_009323. |
| The genetic_marker, MK009992 3_6480367, is located in gene, DCAR_009323. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_009323 | DCAR_009323.mRNA | mRNA | DCARv2_Chr3:6474906..6481526 |
ATGGACTCGCGCGAATCGTCTTCTTCTTCATCTTCACTCCCCGAAGAAGA
CGCCTCTCTCTCTCTAGCCAAACAAGCTTCTCTGCTCTTTCACTCCGCCA
ACTTCGCTGATTGCCTCACTGTCTTGCGTCAGCTTCTGCTCACTAAGCCG
TCCGATCCGAAGGTAATGATTCTGCATACACGCACTTGTATGTGTAGATG
ATGTGGATTTGAATTGGAGTGTGAATTGGTTGTGTTTTAGACTTCGATAG
GGTGTTAGTTGCGGCGAGTTGTGGATTGGTTGAATTGTTTGTTTCGACAA
ATGATTTCTATAATTGGTTCGTATCTACTGTATTGTTTTGTTAGGAGATA
TGTTGAGTTATCCAATAATAAAAAAAACAAGAAATGTTCTGTAAAAGGAA
ATGTTTGTAAGTTGCGGTGGTGTGATGCTCGTTGTGTTTGATGAGCGGTG
TTTGCTGATGATTTTAATGCATGTCCTGAGTGAGATAGTGGCGTGAAGCA
TAACTCATGTGTGTTCATTTACTTTTTATGTTGGATGGCCGAATATCGAC
AAAAATGAAGGCATATTGAAGTAGAAAAATAATAGGAAAGGGAAAGATAT
AATAAATTCTGTGTGCTGCTTGGTGACGGGTAACACCAATCTGTAGTGTG
ACATCTGTGTTCTGACTGAGAGTTCCTTTTTTGGATATTTCACTGCTTTT
CTTAATCAATTGCGTATATTTGTTTGTTACACCTAGTAGTGCATACTTCC
AGTGTCTCAGAAGAGTATATAGTCAGAAGTTATTTGGAGCAAGGCTGAGT
TGTTAATTTGAAGTTGTGGCAGTGCTAACTACCAAGGTTATCACGGTGCC
AAGTCGTGCCTCGGCGCCCGGAACCTTGGAATCGACTAGTCAACAAGTCG
CCCGACTAGTCGTAAAAAGTCGACAAGTCATTCATATACATATTTTTGCT
AGATTTAATAAATAAAATATAGTTGAACACACATTAGTTTATAGTTAAAA
TCAAAAGTAAAAGATAATAAGCAACAGTGAGATGTACAGGACATATCAGA
TTGTTAGTTCACATGTAAAGTGAAGCAAGAAAGTAGTGACAGGAAGGAAA
TAATAATGTGAAGCTGAAATGATACAAAGATAGATTAGGGATATAGTAAT
ATGTTGAAAATGATACAAAGATTACGTAGCAGGAGAAGGTAATTAGGACC
TACACACCGATAACAAGTCTATTAGTCGGACTTCATGTAGCCGCCCAAAG
CCTTATCTTTATTTTCAGAAAAAAATCACATAAGATTTTGCTGAAGTTTT
TGGTCAGACCAGGTACCGGGTCGGGTAAAACTCATTAGTCGCCCGACCAA
ATAAGTCGTTGCTTAGACTTTTTCTATAAAACGGCTTGAACTAGTCGACG
ACTAGTCGTCGACTTGATAACAATGCTAACTACTTACAATTTTTATACAG
AAATTAGTGTCCCAACTTTAATGTTTGGTATAAGATGATGTTAATTTTAT
TGCGGTTTTTGCAGTTTATTTTTAATTGAAATAACTAGACAATTAGAATG
CAAGGCGTTTTCTTTGTAATCCTCTGGCTTCAAGTGATTTCATTTTCGAA
GTAGTATTTTTGTGGCTCTAATTTATATTAATGCTTTCTCTATCTATTCA
TATTTATAATATATCACAGGTTCTCCATAATATAGCTATCGTAGAAAACT
TGCAAGATGGATTTTCAAATCCGAAGAAATTTCTCGAGGTCCTAAATGAT
GTTAAGGTATGTGTTAATATGTTGGTTGTTGCTTTAAAGGTTATAGTCTG
CTTGTTATTGTTCCTTGTAAATGTGTGCGTGTCATTACCTATGTGGGTAG
ATCTACATGAGGTATCAGCATTTTTTATCAGACCATCTCCAACGCATGAC
ACTATTTTGGTGTACCTTTTACACCATTTATAAAAGTACTCTAACCCACT
TGCACTATATAGTACACCAAAAAGAATCTTTCGTCTATAACTAGATATAT
TCCCTCTAAATTTTTCAAAAGTTTTTTGTTTTGGTGTGATATAATAGGAT
ATAAGTGGGACATAGATGGAACAAATTTGTTTTGTTAACAAACAATAGGG
TATACTTGGTATTTTTAAAAACTCTAAAATGGTGTAGAAATTACATCAAA
TGAACATTTGACACCAAATTTGGTGTCAGACTATTCATTTGGTGTAATTC
TTACACCATTTTGGAGTAGCTTTTATTCCAAAAATTACACCATTTTAGAG
TTTTGGGGTTGGGTTGGAGATGTTCTCAGGACCCATTACATGCGTCAATT
TTTTTTTTTTTCCAATCTCTAGGCTTGTTAAGGCTCCTCTTATATTCACC
TTTAAATTTTAACTGAAGTAGATAAAATGAAAAATAAATTGCTTAGGTGA
TTGTAAAGATTATTGAATTTTATTGCATCTTAATTAAAAAGTTCAAATTA
CCACCCTACCACTACCAATCTACACAAGAAACAGACACCATTATGAAATA
TGTGATGATCAAAAAATGAAACAAAATTATAGGTAATAAATAGCTGTTGT
GATGATCAAATGTGAAAAAACAAGAAATACCACTGGTAGAAGAACATATC
TACTTTTTTTCTGCCCTTCAAGAGTTAAAACGATGTTTTATTCAGTTTGT
AATTAAGAAGTGTCAAGTAGAACTTCCTTCTGAAAGTAAGTTTTGTTGAA
ATATGCAAGCTACCCTTAAGAACCTGATTGAATAACAATGTGGTGGGGAA
CGGAAGTGATCATAAAGGAGTACTTATCTACATCTTACTAATTAATGTAA
TATATGTGTTGTTGAAGAGAACATTGTGTGTGTGTGTCTATATATATATA
TATATATATATATATATATATATATGTTTTAATATGTGAAAATGGGATCT
ATTTATTAAGTAGCTTATCTATATATCCCATAATATTTTGAGCCTTAGTT
CATAAATGCCAGGTAATATTGAAAGTATTTTTACTAAAACTTAATTGCAA
GGTAATCAGAAAATCGGGAATATGTGTGTGCATATATATACTTTGTTCAT
TGCTGAGTTGGTGATTATTTATTTGTGCACACGCATCTAGTCTGTTATAA
TTTTAAAATTTAAGGTAAGTGTAAACCGGAAGTTCTCTGTGCAAGTACTC
CATTTATACTACTTCTGTAAATCTCCAAGTTCTTTGGCTTCGAATTTTAT
ATTCCTTTGATGTTTATTTATCTACTTCATTTACAATATTCTTTTTACAC
CCTTTACGTTGCTTTTCAGAAACAAAGTGAAAAGTTTGCAACTGCGTCTG
GTGACAACGTAGTGGGTGCTAACAACTTGGAAAGTGATGTTGTGGCAGTT
TCCAGTGGAAATCCTTGTTTGATACCTCAATTCTCTGCTGCACATAGTTC
CTCAATTGTGCTGAATGCTGAATTTGCCTCTCATGTGGCGTCATACAACA
TAGTATGTTTTGTGAATTTGGCGTGAGATACATCTGAATCATGAAAAGTA
CATTGCTAACGATTTTCATTTTTCTTGTAATTTGATTTCATGCAGGCAGT
CACTTGGTTCAATCTAAGCGAGTATTCAAAGTCGTTTTCTATATTGGAAC
CGGTGTATCAGAAAATTGCACCAATAAGTGAGGTATTATTAATTGTAGCA
GTAGTTTATATTTTGTGCTGAAAAGCTTGAGCAATGCACACAGCAGTGAA
ATTTGAATATCATTTGTTGAAGTTCGGATGTTTAATTGCAGGGTATTGCT
CTACGTATATGCCTTCTGTTGCTGGACGTTGCGTTACTGTCGCACCATGC
CTCAAGATCTGCTGTAAGCTCTCTTTTTGTGAGGCTTATGATTTCTAAAG
CACTTCAATTTTGACATAATGTGGTTGACATTTTGTTTTCTTTACATAAT
GTGGTTAACATTTAGTTTCTGTGCATAATGTGGTTTAGTTTTCTTTACCT
GGTGTAAGGTTTTATGAGTTATGAGTGTCAAATTCCTTAGGTTCATGGCT
TGTCAAAGGGTTGGATTTGGATCGGATTTAGATAAATCCATATCCGAATT
CATTTAATTTTAATGGATGGGGTCGGATCGGATCAAGATTCGGATATATT
TTGGGCAAATCCATATGGAATCCATTTGGCCATCTGGAAAAGAGTTCCAC
ATTGGTGGTTTTAGGCGGCCTTCTTAAATTGTTACTCCCATGGGTGGCAT
CAGTTTCAAACATGTAATATAGTCTAGTAGTTTGCTTTACTCTTGTTTAA
ATGATAGGATGGTCATTGGTCAGTCCCGTATTTTGATTATGTTGGAGATG
TTTGTCATATGGCTTTAGCTTTACTGTTCAGAGTTCTGTTTTGGTTTCCC
ATGTCTTCCATGTTCCAGGTTTTATTCTCTCTTGATAATTTTTTTTATTT
TCCTTTTCCTTTTTTTTTAATTTTTTTTTTCTCCTTGTTGATAAAAATTG
ACTGTCTTATGAGCTCATAAATTTATCTATTATGTTAGCAGACTTTAGTT
GCCCGTATTATATGGAGTAGGAAGGTTTAATTCTTGAGGTTCCGATTGTC
CAAGACACCACACAACGATTCCAACAAACACACTCCCCTGACTCAATGAG
ATGCTCCACACAGAAATTTTAAGTTACCTGATACGTAGACGGCTTAGATG
CTGATTTGTTTTCCTCTTCTTCCCTTGTTTTGCTGCAGGGTATTATTGAG
TATATTGAAAAGATTTCTTCTGTTCGTAGTATGATCAGTCAAAGTGAAAG
TTCGAGCTCTAGTCAGCAACATCCTCCCAATCTAGTGACAAAAGCTTCTT
CAGTTCCTAGCACACCTATTTCTGACATTTCAATTGCTGATTCCGCTACA
AATGCCTCTGAAATTTCTTTATCCAGAAGTTTATCGGAGGAAGCAGAATA
TGAAAACTTGTTCTCTACTCTTGACATGAGTGGACAAAACCTATCAAGAC
CTGTTCACCATTCTTTAAATGATATCTCAAGGACCCAAATTGATGATTCT
ATTCCAATTATTGTTGATCTGAGACTGAAGTTGCATCTGTACAAGGTTCG
ACTTCTTCTCCTTACCAGGAACCTGAAGGCAGCTAAGCGTGAAGTTAAGT
TGGCCATGAACATAGCTAGGGGTAATAATTCTATGGTTCTCTTTTTGAAG
TCTCAGCTTGAATATGCCCGTGGAAATCATCCTAAAGCAATTAAGCTTTT
GATGGCTTCAAGTAATCGAACAGAAATCGGTACTTCTGTAATGTTCTACA
ACAATCTTGGGTGTATCCATTATAGACTTGGCAAGCATCAAACATCGGCT
ATATACTTTTCCAAGGCGTTAAGTACTAGTTCTGTGATACGGAAAGAAAA
GACTCAAAACCACACCCACACCAGTTTTGCAGCGGATAAATCCCTCCTTG
TAGCCTATAACTGTGGCATGCAGTACTTAGCTTGTGGCAAACCAATACTT
GCTGCTCGATTTTTTTACAAAGCCAGCTTAATTTTTTATAATCGGCCTCT
ATTATGGCTACGCATTGCTGAATGTTGTCTGATGGCTCTTGAAAAGGGGC
TGCTGGACTCCACTGGAGCTGCATCCACATCGTCAGAGATTAAGGTTCAT
GTTGTTGGAAAGGGAAAGTGGAGAAATCTTGTTATTGGAGAAGAGGTACC
TAAAAATAGCCGGGCTGATCTCATTGGTCAAAGTGATTCATCGCTTGTTA
ATGATAGGCAGCCTAAACTTTCCATGACCCTGGCACGTCAGTGTCTATTG
AATGCATTACAGCTGCTAAACTCTTCTGAGTCAGTGCACATGAGTTCTGG
TTTGCCATCAGATTTAGCCATTGAGGATGAAACATATTCTAAAAGTACCA
ACTATAAGAGTGTTTCAGGTGGTGATACTCAGGCACATAACCTGGCAGTT
GCTTCAGGTCAGGTTGCAAATGGGGAACTGAAAGAACAGAAGAGCACAAG
TACTCCAAATTCCACCTTGAGAGACTCTATCTCTGATTATGAAGATATAT
GTAGAAAAGAGATCCAGATAATTAGACAATCTATTCTTGTTGATTTGGCA
TATGTAGAGTTGGAGCTAGGCAATCCCTTGAAAGCCTTGTCAACTGCAAG
GTCTCTCCTGAATGTTGCAGAATGCTCCAGAGTGTATATATTCTTGGGTA
ATCTTTATGCAGCTGAGGCTCTGTGCCTACTCAATCGTTTGAAGGAAGCA
GCAGAACATTTGCTGATCTATCTTTCTAGTGGACACAATATTGAACTTCC
ATTTACCCAAGAAGATTGTGGACTGTGGCAAGTAGAAAAAATTGGTGATT
GTGAGGAGTTCAATGGCGGTTCAGTGGCTACCAACATCGCTTCTGATGAA
GGACAAGTTTTTGCACTTAATCCTGAAGAGGCACGTGGAACTCTCTACTC
AAACCTAGCTACAATGTCTGCAGTACAGGGTGATCGTGAGCAGGCTGATA
AATTTGTGAGGCAAGCATTATCTATCTTGCCGAACAGCCCCGAAGCCAAT
TTAACTGCAGTTTACTTGGATCTCATGCTGGGGAAGTCCCGAGAAGCTAT
CTCGAGGTTGAAAAATTGTAGCCATATTACTTTCCTTCCTGGTAGTTTTG
CAGTAACTGGACCGTCATGA