MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPG
YSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEAS
RYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSELSRT
YKSTPYESSIGSGKPDDVFLSMNEMSCGTMTFTSIEDGRD*
| Relationships |
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| The polypeptide, DCAR_021017, derives from mRNA, DCAR_021017. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A1U7ZXV4 ((putative serine/threonine-protein kinase {ECO:0000313|RefSeq:XP_010258651.1}))
HSP 1 Score: 115.546 bits (288), Expect = 2.836e-28
Identity = 72/200 (36.00%), Postives = 103/200 (51.50%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL----------SRTYKSTPYESSIGSGKPDDVFLSMNEMSCGTMTFTSIEDGRD 190
M + ++ + L E ++G+L K I L I+TG S+ P+ +LL W LY+ +++VGLVD +LNG+F EA R++KI LCTQ AP+ RP MS VV ML G+ID++ ISKPGL+S+ + K+T Y SS + + S + SC TMTFT+I D D
Sbjct: 200 MTHVSTRVAGTIGYLAPEYAIRGQLTRKADIYSFGVLLMEIVTGRCSTN-----SKLPVEEQFLLE-----RTWVLYERKELVGLVDTALNGEFDAEEACRFLKIGLLCTQDAPKLRPSMSTVVKMLTGEIDITHKPISKPGLISDFLDLKVRSVENQKNSKNTTYTSSGSDRQYNLSLSSDSTTSCATMTFTAIHDRSD 389
Match: A0A2R6P599 ((LRR receptor-like serine/threonine-protein kinase {ECO:0000313|EMBL:PSR85452.1}))
HSP 1 Score: 116.316 bits (290), Expect = 3.980e-28
Identity = 72/196 (36.73%), Postives = 98/196 (50.00%), Query Frame = 0
Query: 3 KLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSELSRTYKSTPYESSIGSGKPDDVFLSMNEMS--------------CGTMTFTS 184
KLRP MS+VVKMLTGE+++ I KP ++ + F+ D W LY+ +++VGLVDA LNG F EA R++KI LCTQ + RP MS VV ML G++D++E EI+KP L+S+ +K P + KPD + S N S C TMTFT+
Sbjct: 306 KLRPSMSSVVKMLTGEVDINENEITKPALISD-FM--------------------------------DFKTWNLYERRELVGLVDALLNGDFDAEEACRFLKIGLLCTQDTQKLRPSMSSVVKMLTGEVDINENEITKPALISDF-MDFKVKPTPKT----KPDIITASYNHASSDNMENSLLSSGTTCATMTFTA 463
HSP 2 Score: 94.7449 bits (234), Expect = 2.505e-20
Identity = 51/147 (34.69%), Postives = 79/147 (53.74%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL 147
M + ++ + L E ++G+L K + L I++G ++ P+ +LL W LY+ +++VGLVDA LNG F EA R++KI LCTQ + RP MS VV ML G++D++E EI+KP L+S+
Sbjct: 202 MTHVSTRVAGTIGYLAPEYAIRGQLTRKADVYSFGVLLVEIVSGRCNTNS-----RLPVEEKYLLE-----RTWNLYERRELVGLVDALLNGDFDAEEACRFLKIGLLCTQDTQKLRPSMSSVVKMLTGEVDINENEITKPALISDF 338
Match: A0A5C7IC40 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 112.464 bits (280), Expect = 5.505e-28
Identity = 77/185 (41.62%), Postives = 103/185 (55.68%), Query Frame = 0
Query: 7 LMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--SRTYKS--TPYESSIGSGKPDDVFLSMNEMSCGTMTFTSIED 187
++ T+ L E ++G+L K I L I++G ++ + P +LL AWELY+ D+VGLVDASLN +++ EA RY+KIA LCTQ P+ RP MS VV ML G+IDV+E +ISKPGLLSEL + KS Y S +M + S TMTFTSI+D
Sbjct: 102 ILGTIEGYLAPEYAIRGQLTRKADIYSFGVLLLEIVSGRGNTN-----KLLPPEEQYLLE-----RAWELYERGDLVGLVDASLNEDYNVEEAHRYLKIALLCTQDMPKLRPSMSTVVKMLTGEIDVNEEKISKPGLLSELLGLKVKKSDVIKYTDSSKPDHSSSSSGNMYD-SYATMTFTSIDD 275
HSP 2 Score: 52.373 bits (124), Expect = 7.365e-6
Identity = 25/34 (73.53%), Postives = 29/34 (85.29%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPE 34
MPKLRP MSTVVKMLTGEI+V + I+KPG+L E
Sbjct: 198 MPKLRPSMSTVVKMLTGEIDVNEEKISKPGLLSE 231
Match: V4WEI2 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 113.62 bits (283), Expect = 1.321e-27
Identity = 76/177 (42.94%), Postives = 99/177 (55.93%), Query Frame = 0
Query: 18 EIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--SRTYKSTPYESSIGSG---KPDDVFLSMNEM--SCGTMTFTSIED 187
E ++G+L K I L I++G ++ P +LL +AW+LY+ +V LVDASLNG +S+ EA RY+KIA LCTQ P+ RP MS +VNML G+IDV++ EISKPGLLS+L R K ++S SG K D S M S TMTF SI D
Sbjct: 219 EYAIRGQLTRKADIYSFGVLLLEIVSGRCNTN-----RRLPYEEQYLLE-----MAWKLYERGKLVELVDASLNGDYSVEEAHRYLKIALLCTQDMPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSYATMTFNSIFD 385
HSP 2 Score: 49.2914 bits (116), Expect = 1.226e-4
Identity = 27/72 (37.50%), Postives = 42/72 (58.33%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRR---------NIITGDASSPGYSSIEASPMPSTW 63
MPK RP MST+V ML GEI+V + I+KPG+L + R + ++GD+S +SS + M +++
Sbjct: 304 MPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSY 375
Match: A0A067EAT9 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011} (Fragment))
HSP 1 Score: 114.005 bits (284), Expect = 1.697e-27
Identity = 77/180 (42.78%), Postives = 100/180 (55.56%), Query Frame = 0
Query: 15 LTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--SRTYKSTPYESSIGSG---KPDDVFLSMNEM--SCGTMTFTSIED 187
L E ++G+L K I L I++G ++ P +LL +AW+LY+ +V LVDASLNG +S+ EA RY+KIA LCTQ P+ RP MS +VNML G+IDV++ EISKPGLLS+L R K ++S SG K D S M S TMTF SI D
Sbjct: 243 LAPEYAIRGQLTRKADIYSFGVLLLEIVSGRCNTN-----RRLPYEEQYLLE-----MAWKLYERGKLVELVDASLNGDYSVEEAHRYLKIALLCTQDMPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSYATMTFNSIFD 412
HSP 2 Score: 49.2914 bits (116), Expect = 1.356e-4
Identity = 27/72 (37.50%), Postives = 42/72 (58.33%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRR---------NIITGDASSPGYSSIEASPMPSTW 63
MPK RP MST+V ML GEI+V + I+KPG+L + R + ++GD+S +SS + M +++
Sbjct: 331 MPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSY 402
Match: A0A2H5QLI1 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 113.62 bits (283), Expect = 2.719e-27
Identity = 77/180 (42.78%), Postives = 100/180 (55.56%), Query Frame = 0
Query: 15 LTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--SRTYKSTPYESSIGSG---KPDDVFLSMNEM--SCGTMTFTSIED 187
L E ++G+L K I L I++G ++ P +LL +AW+LY+ +V LVDASLNG +S+ EA RY+KIA LCTQ P+ RP MS +VNML G+IDV++ EISKPGLLS+L R K ++S SG K D S M S TMTF SI D
Sbjct: 270 LAPEYAIRGQLTRKADIYSFGVLLLEIVSGRCNTN-----RRLPYEEQYLLE-----MAWKLYERGKLVELVDASLNGDYSVEEAHRYLKIALLCTQDMPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSYATMTFNSIFD 439
HSP 2 Score: 48.521 bits (114), Expect = 2.135e-4
Identity = 27/72 (37.50%), Postives = 42/72 (58.33%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRR---------NIITGDASSPGYSSIEASPMPSTW 63
MPK RP MST+V ML GEI+V + I+KPG+L + R + ++GD+S +SS + M +++
Sbjct: 358 MPKRRPSMSTLVNMLIGEIDVNKEEISKPGLLSDLLGLRVDKGEKDKASAVSGDSSKLDHSSFSSGNMTTSY 429
Match: A0A7J7C2A8 ((Putative serine/threonine-protein kinase isoform X3 {ECO:0000313|EMBL:KAF5728055.1}))
HSP 1 Score: 106.686 bits (265), Expect = 3.242e-27
Identity = 58/127 (45.67%), Postives = 79/127 (62.20%), Query Frame = 0
Query: 70 DILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--------SRTYKSTPYESSIGSGKPDDVFLSMNEMSC-GTMTFTSIED 187
++ AWELY++ ++ LVD S+N + + EA RY+KI CTQ P+ RP MS VV ML G+IDV++ +IS+PGLL EL S +T + +S GSGKP+ F S+ S TMTF SI D
Sbjct: 7 NLQAWELYEKGELASLVDTSMNRDYDVEEACRYMKIGLCCTQDKPKLRPSMSTVVQMLMGEIDVNDTKISRPGLLYELMGLKGPKNSSDTMNTTFTNSAGSGKPETSFSSLGMTSSHATMTFNSIYD 133
HSP 2 Score: 45.8246 bits (107), Expect = 4.980e-4
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 0
Query: 2 PKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPE 34
PKLRP MSTVV+ML GEI+V I++PG+L E
Sbjct: 51 PKLRPSMSTVVQMLMGEIDVNDTKISRPGLLYE 83
Match: A0A1S3XAU0 ((probable LRR receptor-like serine/threonine-protein kinase At1g56140 {ECO:0000313|RefSeq:XP_016437026.1}))
HSP 1 Score: 113.235 bits (282), Expect = 4.212e-27
Identity = 71/177 (40.11%), Postives = 107/177 (60.45%), Query Frame = 0
Query: 15 LTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSEL--SRTYKSTPYES-SIGSGKPDDVFLSMN-EMSCGTMTFTSIED 187
L E ++G+L K + L I++G ++ + P+ +LL AW+LY++ +++ LVD SL F+++EA R++K++ LCTQ P+SRP MS VV +L G+++V EIS+PG+LS+L R+ K+T +S S GSGK D SMN M+ GTMTFTSI D
Sbjct: 275 LAPEYAIRGQLTRKADVYSFGILLLEIVSGRCNTN-----KRLPIEQQYLLE-----RAWKLYKKGELIELVDTSLGDDFNVDEACRFLKVSLLCTQVMPKSRPSMSTVVKLLIGEMEVDAEEISEPGMLSDLLSLRSNKNTSSDSLSAGSGKQVDSSSSMNTTMTHGTMTFTSIND 441
HSP 2 Score: 44.2838 bits (103), Expect = 5.304e-3
Identity = 23/41 (56.10%), Postives = 30/41 (73.17%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAF-LRRN 40
MPK RP MSTVVK+L GE+EV + I++PG+L + LR N
Sbjct: 363 MPKSRPSMSTVVKLLIGEMEVDAEEISEPGMLSDLLSLRSN 403
Match: A0A6A4LMA9 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011} (Fragment))
HSP 1 Score: 107.457 bits (267), Expect = 5.251e-27
Identity = 69/184 (37.50%), Postives = 96/184 (52.17%), Query Frame = 0
Query: 15 LTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSE-LSRTYKSTP----------YESSIGSGKPDDVFLSMNEMSCGTMTFTSIED 187
L E ++G+L K + L I+TG ++ P+ +LL WELY+ +++VGLVD SLNG+F A R++KI LCTQ AP RP MS VV ML G+ID+ EI+KP L+S+ + K+TP Y + SG ++ LS S TMTFT+I D
Sbjct: 3 LAPEYAIRGQLTRKADVYSFGVLLVEIVTGRCNTNN-----RLPIAEQYLLE-----RTWELYERRELVGLVDVSLNGEFDAEVACRFLKIGLLCTQDAPNLRPSMSTVVKMLTGEIDIEGKEITKPALISDFMDLKVKTTPKAMPDKTNSSYIDTSSSGNLENSLLSSGMTSVPTMTFTAIYD 176
HSP 2 Score: 48.1358 bits (113), Expect = 1.320e-4
Identity = 21/33 (63.64%), Postives = 27/33 (81.82%), Query Frame = 0
Query: 2 PKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPE 34
P LRP MSTVVKMLTGEI+++GK I KP ++ +
Sbjct: 92 PNLRPSMSTVVKMLTGEIDIEGKEITKPALISD 124
Match: A0A7J7HAV3 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 112.464 bits (280), Expect = 6.182e-27
Identity = 67/198 (33.84%), Postives = 105/198 (53.03%), Query Frame = 0
Query: 1 MPKLRPLMSTVVKMLTGEIEVQGKLIAKPGILPEAFLRRNIITGDASSPGYSSIEASPMPSTWLLNIASDILAWELYQEQDIVGLVDASLNGKFSLNEASRYIKIAFLCTQAAPRSRPLMSDVVNMLKGDIDVSEMEISKPGLLSE-----LSRTYKSTP------YESSIGSGKPDDVFLSMNEMSCGTMTFTSIED 187
M + ++ + L E ++G+L K I L I+TG ++ P+ +LL W+L++ +++VGLVD +LNG F EA R++KI LCTQ P+ RP MS VV ML G++D++E EI+KP L+++ + K+TP Y + SG +++ +S SC TMTFT+I D
Sbjct: 236 MTHVSTRVAGTIGYLAPEYAIRGQLTRKADIYSFGVLLVEIVTGRCNTN-----TRLPVEEQYLLE-----RTWDLFERRELVGLVDTALNGDFDAEEACRFLKIGLLCTQDTPKLRPSMSTVVKMLIGEVDINENEITKPALITDFMDLKVKSARKATPDVNNINYNFASSSGNLENLTMSSGTSSCATMTFTAICD 423
| Match Name | Stats | Description |
|---|---|---|
| A0A1U7ZXV4 | E-Value: 2.836e-28, PID: 36.00 | (putative serine/threonine-protein kinase {ECO:000... [more] |
| A0A2R6P599 | E-Value: 3.980e-28, PID: 36.73 | (LRR receptor-like serine/threonine-protein kinase... [more] |
| A0A5C7IC40 | E-Value: 5.505e-28, PID: 41.62 | Protein kinase domain-containing protein {ECO:0000... [more] |
| V4WEI2 | E-Value: 1.321e-27, PID: 42.94 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A067EAT9 | E-Value: 1.697e-27, PID: 42.78 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A2H5QLI1 | E-Value: 2.719e-27, PID: 42.78 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A7J7C2A8 | E-Value: 3.242e-27, PID: 45.67 | (Putative serine/threonine-protein kinase isoform ... [more] |
| A0A1S3XAU0 | E-Value: 4.212e-27, PID: 40.11 | (probable LRR receptor-like serine/threonine-prote... [more] |
| A0A6A4LMA9 | E-Value: 5.251e-27, PID: 37.50 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A7J7HAV3 | E-Value: 6.182e-27, PID: 33.84 | Protein kinase domain-containing protein {ECO:0000... [more] |
Pages
back to top| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |