MAGALLFSMMIITITITITILLTFLKPTHSGKTTTIFSSTSFSSNNHYNV
PALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPV
DYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHI
SFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRN
VSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYY
LWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEG
SMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQ
FHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK*
| Relationships |
|---|
| The polypeptide, DCAR_028400, derives from mRNA, DCAR_028400. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A4S4DYL9 ((Uncharacterized protein {ECO:0000313|EMBL:THG08549.1}))
HSP 1 Score: 602.823 bits (1553), Expect = 0.000e+0
Identity = 277/364 (76.10%), Postives = 317/364 (87.09%), Query Frame = 0
Query: 25 LKPTHSGKTTTIFSSTSFSSNNHYNVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
+ P+ S T ++TS VPALFVIGDSSVD GTNNFLGTLARADRLPYGRDFDTH+PTGRF NGRIPVDYLA RLGLP+VP+YLGQTGSV+DM+HGVNYASAGAG+IF+SGSELGQHISFTQQ+QQVTDT QQFTLNMGE A LIS S+FYISIGTNDYIHYYLRNVS+VQSLYLPW FNQFL T+K+EI+NLY ANVRKVVV GL PIGCAPYYLW Y+S NG+CVQMINDMI E NF +RY+V+ L+Q+L DADIIFCD+FE SMDIIKN+DR+GFNVTT+ACCGLG+YKG I C++PEM+C NASNH+WWDQFHPTD VNAILADNVWS +HT++CYP +LQ M+A+K +
Sbjct: 46 MSPSQSRHPLTYPTATS-----SLPVPALFVIGDSSVDCGTNNFLGTLARADRLPYGRDFDTHQPTGRFCNGRIPVDYLAKRLGLPFVPSYLGQTGSVEDMIHGVNYASAGAGIIFSSGSELGQHISFTQQIQQVTDTIQQFTLNMGEDAATKLISNSVFYISIGTNDYIHYYLRNVSNVQSLYLPWSFNQFLATTVKQEIKNLYTANVRKVVVMGLPPIGCAPYYLWSYQSENGECVQMINDMILELNFVLRYIVEELSQELIDADIIFCDVFEASMDIIKNNDRYGFNVTTDACCGLGKYKGWIMCISPEMACSNASNHLWWDQFHPTDAVNAILADNVWSGLHTEICYPMNLQNMIAQKAR 404
Match: A0A7J7GF07 ((Uncharacterized protein {ECO:0000313|EMBL:KAF5938531.1}))
HSP 1 Score: 600.512 bits (1547), Expect = 0.000e+0
Identity = 276/364 (75.82%), Postives = 317/364 (87.09%), Query Frame = 0
Query: 25 LKPTHSGKTTTIFSSTSFSSNNHYNVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
+ P+ S T ++TS VPALFVIGDSSVD GTNNFLGT ARADRLPYGRDFDTH+PTGRF NGRIPVDYLA RLGLP+VP+YLGQTGSV+DM+HGVNYASAGAG+IF+SGSELGQHISFTQQ+QQVTDT QQFTLNMGE VA LIS S+FYISIGTNDYIHYYLRNVS+VQSLYLPW FNQFL T+K+EI+NLY ANVRKVVV GL PIGCAPYYLW Y+S NG+CVQMINDMI E NF +RY+V+ L+Q+L +ADIIFCD+FE SMDIIKN+DR+GFNVTT+ACCGLG+YKG I C++PEM+C NASNH+WWDQFHPTD VNAILADNVWS +HT++CYP +LQ M+A+K +
Sbjct: 46 MSPSQSRHPLTYPTATS-----SLPVPALFVIGDSSVDCGTNNFLGTFARADRLPYGRDFDTHQPTGRFCNGRIPVDYLAKRLGLPFVPSYLGQTGSVEDMIHGVNYASAGAGIIFSSGSELGQHISFTQQIQQVTDTIQQFTLNMGEDVATKLISNSVFYISIGTNDYIHYYLRNVSNVQSLYLPWSFNQFLATTVKQEIKNLYNANVRKVVVMGLPPIGCAPYYLWSYQSENGECVQMINDMILELNFVLRYIVEELSQELIEADIIFCDVFEASMDIIKNNDRYGFNVTTDACCGLGKYKGWIMCISPEMACNNASNHLWWDQFHPTDAVNAILADNVWSGLHTEICYPMNLQNMIAQKAR 404
Match: A0A2G9GJG1 ((Triacylglycerol lipase {ECO:0000313|EMBL:PIN05431.1}) (3.1.1.3 {ECO:0000313|EMBL:PIN05431.1}))
HSP 1 Score: 588.571 bits (1516), Expect = 0.000e+0
Identity = 265/340 (77.94%), Postives = 305/340 (89.71%), Query Frame = 0
Query: 49 NVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
+VPALFVIGDSSVD GTNNFLGT ARADR PYGRDFDTH+PTGRF NGRIPVDYLA+RLGLP+VP+YLGQTGSVDDM+ GVNYASAGAG+IF+SGSELGQHIS TQQ+QQV DTFQQF L+MGE A D ISKS+FYISIG+NDYIHYYLRNVSSVQSLYLPW FNQFL QT+K+EI+NLY ANVR VVV GLAPIGCAPYYLW Y+S+NG+C++MINDMI EFNF R+MV+ LNQ+L +A+I+FCD FEGSMDIIKN++R+GFNVT +ACCGLG+Y G I C++ +M+C NASNHIWWDQFHPTD VN ILADNVWSS+HT MCYP +LQEM+A+ +K
Sbjct: 70 HVPALFVIGDSSVDCGTNNFLGTFARADRPPYGRDFDTHQPTGRFCNGRIPVDYLALRLGLPFVPSYLGQTGSVDDMIQGVNYASAGAGVIFSSGSELGQHISLTQQIQQVMDTFQQFILSMGEDAANDRISKSIFYISIGSNDYIHYYLRNVSSVQSLYLPWSFNQFLAQTIKQEIQNLYNANVRNVVVMGLAPIGCAPYYLWLYQSKNGECIKMINDMILEFNFVTRFMVEELNQELTEANIVFCDAFEGSMDIIKNYNRYGFNVTADACCGLGKYGGWIMCISSDMACSNASNHIWWDQFHPTDAVNEILADNVWSSLHTSMCYPMNLQEMIAKNSK 409
Match: A0A2R6PIG4 ((GDSL esterase/lipase {ECO:0000313|EMBL:PSR91430.1}))
HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 271/369 (73.44%), Postives = 314/369 (85.09%), Query Frame = 0
Query: 21 LLTFLKPTHS-GKTTTIFSSTSFSSNNHYNVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
L+ PT S + + ST+ SS++ VPALFVIGDSSVD GTNNFLGT ARADRLPYGRDFDTH+PTGRF NGR+PVDYLA RLGLP+VP+Y+GQTG V+DM+HGVNYASAGAG+IF+SGSELGQHISFTQQ+QQV DT++QFTL+MGE AA+LIS S+FYISIGTNDYIHYYLRNVS+VQSLYLPW FNQFL TMK+EI+NLY NVRKVVV GLAPIGCAPYYLWQY NG+CVQMINDMI EFNF VRYMV+ L+ +L DADIIFCD FE SMDII N+ R+GFNVT +ACCGLG+Y G I C++PEM+C NASNHIWWDQFHPTD VNAILADNVWS +H ++CYP +LQ ++ +K +
Sbjct: 38 LVALNSPTMSPSEPPNLQISTTLSSSSPI-VPALFVIGDSSVDCGTNNFLGTFARADRLPYGRDFDTHQPTGRFCNGRVPVDYLAERLGLPFVPSYIGQTGPVEDMIHGVNYASAGAGIIFSSGSELGQHISFTQQIQQVMDTYEQFTLSMGEDAAAELISNSVFYISIGTNDYIHYYLRNVSNVQSLYLPWSFNQFLASTMKQEIKNLYNTNVRKVVVMGLAPIGCAPYYLWQYERENGECVQMINDMILEFNFVVRYMVEELSLELVDADIIFCDAFEASMDIIMNNKRYGFNVTADACCGLGKYAGWIMCISPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHKEICYPMNLQNIITQKDR 405
Match: A0A1U7WLR3 ((GDSL esterase/lipase At5g08460 {ECO:0000313|RefSeq:XP_009775205.1}))
HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 264/339 (77.88%), Postives = 301/339 (88.79%), Query Frame = 0
Query: 50 VPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
VPALFVIGDSSVD GTNNFLGT ARADRLPYGRDFDTH+PTGRF NGRIPVDYLA+RLGLP+VP+YLGQ GS++DM+ GVNYASAGAG+IF+SGSELGQHIS QQ++QVTDT QQF + +GE ADLIS S+FYISIGTNDYIHYYLRNVS +QS+YLPW FNQFL QTMK+EI+NLY ANVRKVVV GLAPIGCAPYYLW Y S+NG+CV+ INDMI EFNFAVRY+V LN++L A +IFCD FEGSMDII+NHDR+GFNVT EACCGLG+Y+G I C++PEM+C NASNHIWWDQFHPTD VNAILADNVWS +HT MCYP +LQ+MLA+ T+
Sbjct: 63 VPALFVIGDSSVDCGTNNFLGTFARADRLPYGRDFDTHQPTGRFCNGRIPVDYLAVRLGLPFVPSYLGQAGSIEDMILGVNYASAGAGIIFSSGSELGQHISLAQQIEQVTDTIQQFIMTIGEEATADLISTSVFYISIGTNDYIHYYLRNVSDIQSVYLPWSFNQFLAQTMKQEIKNLYNANVRKVVVMGLAPIGCAPYYLWLYHSKNGQCVETINDMILEFNFAVRYIVAELNEELVGATVIFCDAFEGSMDIIQNHDRYGFNVTDEACCGLGKYRGWILCISPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHTPMCYPMNLQDMLAQSTR 401
Match: A0A7J0H037 ((SGNH hydrolase-type esterase superfamily protein {ECO:0000313|EMBL:GFZ16391.1}))
HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 267/363 (73.55%), Postives = 310/363 (85.40%), Query Frame = 0
Query: 26 KPTHSGKTTTIFSSTSFSSNNHYNVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
+P++ + + SS+S VPALFVIGDSSVD GTNNFLGT ARADRLPYGRDFDTH+PTGRF NGR+PVDYLA RLGLP+VP+Y+GQTG V+DM+HGVNYASAGAG+IF+SGSELGQHISFTQQ+QQV DT++QFTL+MGE AA+LIS S+FYISIGTNDYIHYYLRNVS+VQSLYLPW FNQFL TMK+EI+NLY NVRKVVV GLAPIGCAPYYLWQY NG+CVQMINDMI EFNF VRYMV+ L+ +L DADIIFCD FE SMDII N+ R+GFNVT +ACCGLG+Y G I C++PEM+C NASNHIWWDQFHPTD VNAILADNVWS +H ++CYP +LQ ++ +K +
Sbjct: 50 EPSNPQISPALNSSSSI-------VPALFVIGDSSVDCGTNNFLGTFARADRLPYGRDFDTHQPTGRFCNGRVPVDYLAERLGLPFVPSYIGQTGPVEDMIHGVNYASAGAGIIFSSGSELGQHISFTQQIQQVMDTYEQFTLSMGEDAAAELISNSVFYISIGTNDYIHYYLRNVSNVQSLYLPWSFNQFLASTMKQEIKNLYNTNVRKVVVMGLAPIGCAPYYLWQYERENGECVQMINDMILEFNFVVRYMVEELSLELVDADIIFCDAFEASMDIIMNNKRYGFNVTADACCGLGKYAGWIMCISPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHKEICYPMNLQNIITQKGR 405
Match: A0A314LAP2 ((Gdsl esteraselipase {ECO:0000313|EMBL:OIT38563.1}))
HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0
Identity = 264/339 (77.88%), Postives = 300/339 (88.50%), Query Frame = 0
Query: 50 VPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
VPALFVIGDSSVD GTNNFLGT ARADRLPYGRDFDTH+PTGRF NGRIPVDYLA RLGLP+VP+YLGQ GS++DM+ GVNYASAGAG+IF+SGSELGQHIS QQ++QVTDT QQF + +GE ADLIS S+FYISIGTNDYIHYYLRNVS VQS++LPW FNQFL QTMK+EI+NLY ANVRKVVV GLAPIGCAPYYLW Y S+NG+C++ INDMI EFNFAVRY+V LN++L A +IFCD FEGSMDII+NHDR+GFNVT EACCGLG+YKG I C++PEM+C NASNHIWWDQFHPTD VNAILADNVWS +HT MCYP +LQ+MLA+ T+
Sbjct: 63 VPALFVIGDSSVDCGTNNFLGTFARADRLPYGRDFDTHQPTGRFCNGRIPVDYLAARLGLPFVPSYLGQAGSIEDMILGVNYASAGAGIIFSSGSELGQHISLAQQIEQVTDTIQQFIMTIGEEATADLISTSVFYISIGTNDYIHYYLRNVSDVQSVFLPWSFNQFLAQTMKQEIKNLYNANVRKVVVMGLAPIGCAPYYLWLYHSKNGQCIESINDMILEFNFAVRYIVAELNEELVGATVIFCDAFEGSMDIIQNHDRYGFNVTDEACCGLGKYKGWILCISPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHTPMCYPMNLQDMLAQSTR 401
Match: A0A6P6TMQ5 ((GDSL esterase/lipase At5g08460-like {ECO:0000313|RefSeq:XP_027079789.1}))
HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 260/337 (77.15%), Postives = 302/337 (89.61%), Query Frame = 0
Query: 50 VPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARK 386
VPALFVIGDSSVD GTNNFLGT ARADRLPYG+DFDTH+PTGRF NGRIPVDY+A+RLGLP+VP+YLGQ GS++DM+ GVNYASAGAG+IF+SGSELGQHIS TQQ+QQV DT QQF LN+GE ADL+S+S+FYISIG+NDYIHYYLRNVS+VQS YLPW FNQFL TMK+EI+NLY ANVRKVVV GLAP+GCAPYYLW Y S++G+C++ INDMI EFN+A+RYMVD LNQ+L D++IIFCD FE SMDIIKNHD +GFNVT ACCGLG+YKG ITC+TPEM+C NASNHIWWDQFHPTD VNAILADNVWS +HT +CYP +L++M+A+K
Sbjct: 69 VPALFVIGDSSVDCGTNNFLGTFARADRLPYGKDFDTHQPTGRFCNGRIPVDYIALRLGLPFVPSYLGQGGSIEDMMKGVNYASAGAGIIFSSGSELGQHISLTQQIQQVYDTNQQFILNIGEDATADLMSRSIFYISIGSNDYIHYYLRNVSNVQSAYLPWGFNQFLADTMKEEIKNLYTANVRKVVVMGLAPLGCAPYYLWMYGSKHGRCIEKINDMIMEFNYAMRYMVDELNQELMDSNIIFCDAFEASMDIIKNHDHYGFNVTANACCGLGKYKGWITCLTPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHTNICYPMNLKDMIAQK 405
Match: A0A6P6TWZ2 ((GDSL esterase/lipase At5g08460-like {ECO:0000313|RefSeq:XP_027082860.1}))
HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 260/337 (77.15%), Postives = 302/337 (89.61%), Query Frame = 0
Query: 50 VPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYLAMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARK 386
VPALFVIGDSSVD GTNNFLGT ARADRLPYG+DFDTH+PTGRF NGRIPVDY+A+RLGLP+VP+YLGQ GS++DM+ GVNYASAGAG+IF+SGSELGQHIS TQQ+QQV DT QQF LN+GE ADL+S+S+FYISIG+NDYIHYYLRNVS+VQS YLPW FNQFL TMK+EI+NLY ANVRKVVV GLAP+GCAPYYLW Y S++G+C++ INDMI EFN+A+RYMVD LNQ+L D++IIFCD FE SMDIIKNHD +GFNVT ACCGLG+YKG ITC+TPEM+C NASNHIWWDQFHPTD VNAILADNVWS +HT +CYP +L++M+A+K
Sbjct: 69 VPALFVIGDSSVDCGTNNFLGTFARADRLPYGKDFDTHQPTGRFCNGRIPVDYIALRLGLPFVPSYLGQGGSIEDMMKGVNYASAGAGIIFSSGSELGQHISLTQQIQQVYDTNQQFILNIGEDATADLMSRSIFYISIGSNDYIHYYLRNVSNVQSAYLPWGFNQFLADTMKEEIKNLYTANVRKVVVMGLAPLGCAPYYLWMYGSKHGRCIEKINDMIMEFNYAMRYMVDELNQELMDSNIIFCDAFEASMDIIKNHDHYGFNVTANACCGLGKYKGWITCLTPEMACSNASNHIWWDQFHPTDAVNAILADNVWSGLHTNICYPMNLEDMIAQK 405
Match: A0A5J4ZV13 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8520961.1}))
HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 265/351 (75.50%), Postives = 304/351 (86.61%), Query Frame = 0
Query: 47 HYNVPALFVIGDSSVDSGTNNFLGTLARADRLPYGRDFDTHRPTGRFSNGRIPVDYL---------AMRLGLPYVPTYLGQTGSVDDMVHGVNYASAGAGLIFTSGSELGQHISFTQQVQQVTDTFQQFTLNMGEVVAADLISKSLFYISIGTNDYIHYYLRNVSSVQSLYLPWKFNQFLVQTMKKEIENLYIANVRKVVVAGLAPIGCAPYYLWQYRSRNGKCVQMINDMITEFNFAVRYMVDGLNQKLQDADIIFCDIFEGSMDIIKNHDRFGFNVTTEACCGLGRYKGVITCMTPEMSCKNASNHIWWDQFHPTDTVNAILADNVWSSMHTKMCYPESLQEMLARKTK 388
H VPALFVIGDSSVD GTNNFLGT ARADR PYGRDFDTH+PTGRF NGRIPVDYL A+RLGLP+VP+YLGQT SV++M+HGVNYASAGAG+IF+SGSELGQHISFTQQ+QQV DTFQQF L+MGE AA LIS+S+FYISIG NDYIHYYLRNVS+VQSLY PW FNQFL TM++EI+NLY NVR VVV GLAPIGCAPYYL Y+S NG+CVQMINDMI EFNF +RY V+ L++ L DA+IIFCD+ +GSMDIIKNH+++GFN+TT+ACCGLGRY+G I C++PEM+C NASNHIWWDQFHPTD VNAILADNVWSS+HTKMCYP +LQ+ML+ K K
Sbjct: 79 HPLVPALFVIGDSSVDCGTNNFLGTFARADRFPYGRDFDTHQPTGRFCNGRIPVDYLELCFDYFEPALRLGLPFVPSYLGQTSSVEEMIHGVNYASAGAGIIFSSGSELGQHISFTQQIQQVMDTFQQFILSMGEDEAAKLISESIFYISIGINDYIHYYLRNVSNVQSLYPPWSFNQFLAHTMRQEIKNLYDTNVRNVVVMGLAPIGCAPYYLSLYQSENGECVQMINDMILEFNFVMRYTVEELSKDLVDANIIFCDVLQGSMDIIKNHEQYGFNITTDACCGLGRYRGWIMCISPEMACSNASNHIWWDQFHPTDAVNAILADNVWSSLHTKMCYPMNLQDMLSEKAK 429
| Match Name | Stats | Description |
|---|---|---|
| A0A4S4DYL9 | E-Value: 0.000e+0, PID: 76.10 | (Uncharacterized protein {ECO:0000313|EMBL:THG0854... [more] |
| A0A7J7GF07 | E-Value: 0.000e+0, PID: 75.82 | (Uncharacterized protein {ECO:0000313|EMBL:KAF5938... [more] |
| A0A2G9GJG1 | E-Value: 0.000e+0, PID: 77.94 | (Triacylglycerol lipase {ECO:0000313|EMBL:PIN05431... [more] |
| A0A2R6PIG4 | E-Value: 0.000e+0, PID: 73.44 | (GDSL esterase/lipase {ECO:0000313|EMBL:PSR91430.1... [more] |
| A0A1U7WLR3 | E-Value: 0.000e+0, PID: 77.88 | (GDSL esterase/lipase At5g08460 {ECO:0000313|RefSe... [more] |
| A0A7J0H037 | E-Value: 0.000e+0, PID: 73.55 | (SGNH hydrolase-type esterase superfamily protein ... [more] |
| A0A314LAP2 | E-Value: 0.000e+0, PID: 77.88 | (Gdsl esteraselipase {ECO:0000313|EMBL:OIT38563.1}... [more] |
| A0A6P6TMQ5 | E-Value: 0.000e+0, PID: 77.15 | (GDSL esterase/lipase At5g08460-like {ECO:0000313|... [more] |
| A0A6P6TWZ2 | E-Value: 0.000e+0, PID: 77.15 | (GDSL esterase/lipase At5g08460-like {ECO:0000313|... [more] |
| A0A5J4ZV13 | E-Value: 0.000e+0, PID: 75.50 | (Uncharacterized protein {ECO:0000313|EMBL:KAA8520... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |