DCAR_028537

Resource Type: 
Polypeptide
Name: 
DCAR_028537
Identifier: 
DCAR_028537.mRNA-protein
Sequence: 
MRWSNSTVCLPVPVSSEAAEDSTAALLKSCRDSEVTELGAWDGIYKEASN
GSFGVSNGIVAVCLPVPVSSEAAEDSTAALLKSCSYGDDYKIYQPDCLTI
PATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELST
DELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTR
GFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMH
KRKLIGGREFPEWVKASDRKKLLQVNGVVADLVVDVNGYGNFTTLSDAIK
AVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSF
IGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLY
RCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKG
LPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGR
PWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGL
GNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV*
Sequence Length: 
597
Sequence Checksum: 
1b8a65fb147bd86ddb367f88f65fcb5b
View location in JBrowse: 
DCARv2_Chr8:5814147..5821293+
Organism: 
Daucus carota subspecies sativus
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_028537, derives from mRNA, DCAR_028537.
Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6A4L156 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}) (Fragment))

HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0
Identity = 351/511 (68.69%), Postives = 411/511 (80.43%), Query Frame = 0
 
Query:   89 DYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE---FPEWVKASDRKKLLQVNGVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            D    QP+CL +P   F SS+K+TIDVV+QV S+VS+F+  F DFRLSNAISDCLDLL+LS DEL  TLL +QNP N ++N TGNLSADL TWLS AL NQDTC+EGF+GT   V++LVA GL +++SL++ IL NV    +   G    H ++G        RKL+  R+   +P W +  DRK L  VNGV AD+VV  +G GNFT ++DAI A PDYST+R VIY+KKGVY EY+EI KKKWN+M+VGDGMDVT+ISGN +FI GWTTYRSATF VKG+GFIARDMTFENTAGP+ HQAVAFRSDSDLS L+RCA+RGYQDTLYAHS RQFYREC ITGTVDFIFGD   VFQ CQILARKGLPNQKNTITAQGRK+  E TGFSIQF NIS EP+++++   NST TYLGRPWKLYSRTV MQSY+SNAIRPEGWLEWN  FALDTL+YGEYMNYGPGAG+G+RVKWPG+R LN +++ + FTVAQF++GN WLPSTGVKYTAGLVV
Sbjct:   31 DSDFLQPECLKVPTGVFASSVKSTIDVVEQVASLVSKFAPAFGDFRLSNAISDCLDLLDLSADELTRTLLASQNP-NGKNNGTGNLSADLTTWLSGALGNQDTCIEGFEGTNRIVQNLVAGGLNQVTSLIKDILTNVAYGVNNDGGG---HPQQG--------RKLVSKRDAHSYPYWFRHEDRKMLQVVNGVSADVVVAKDGTGNFTNVTDAISAAPDYSTSRFVIYIKKGVYNEYIEIGKKKWNLMMVGDGMDVTVISGNQNFIDGWTTYRSATFAVKGQGFIARDMTFENTAGPQKHQAVAFRSDSDLSALFRCAIRGYQDTLYAHSLRQFYRECQITGTVDFIFGDGKVVFQNCQILARKGLPNQKNTITAQGRKDPTEATGFSIQFCNISGEPDLLASL--NSTSTYLGRPWKLYSRTVFMQSYMSNAIRPEGWLEWNGDFALDTLFYGEYMNYGPGAGIGSRVKWPGFRALNDSAQVSNFTVAQFIVGNLWLPSTGVKYTAGLVV 527
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YM53 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 717.613 bits (1851), Expect = 0.000e+0
Identity = 355/516 (68.80%), Postives = 418/516 (81.01%), Query Frame = 0
 
Query:   85 SYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE-FPEWVKASDRKKLLQVNG---VVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            S G DY     +CL +P +EF+SSLK+TIDVVQQVTSIVS+F+  F DFRLSNAISDCLDLL+LS+DEL  TL  +QNP + +DNSTG+LSADL+TWLSAAL NQDTC+EGF+G+   V +LV+ GL +++SLV  +L  V+   +  + S   +   G  PG    RKL    + FP WVK +D ++LLQVNG   V AD VV  +G GNFT + DAI A PD++T R VIY+KKGVY+EYVEISKKK N+M++GDGMDVT+ISGN S I GWTTYRSATF VKG+GFIARD+TFENTAGP+ HQAVAFRSDSDLSV++RCA+RGYQDTLYAHS RQFYRECHITGTVDFIFGD+ AVFQ CQILARKGLPNQKNTITAQGRK+  E TGFSIQFSNIS EP+V+++   NST TYLGRPWK YSRTV MQSY+ +AIRPEGWLEWN +FALDTL YGEYMNYGPGA L +RVKWPG+  +N T++AN FTVAQF+LGNSWLPSTGVKYTAGL +
Sbjct:   23 SSGPDY--LDAECLEVPVSEFVSSLKSTIDVVQQVTSIVSKFANAFGDFRLSNAISDCLDLLDLSSDELSWTLSASQNP-SGKDNSTGHLSADLRTWLSAALINQDTCMEGFEGSNSIVMNLVSGGLNQVTSLVHDVLTMVRPVPNTNTKS---NSEGGGHPG----RKLKNNSDHFPYWVKPNDHRRLLQVNGSAGVTADAVVAADGTGNFTRIMDAISAAPDHTTRRYVIYIKKGVYREYVEISKKKRNLMMIGDGMDVTVISGNRSVIDGWTTYRSATFAVKGQGFIARDITFENTAGPQKHQAVAFRSDSDLSVVFRCAIRGYQDTLYAHSMRQFYRECHITGTVDFIFGDSTAVFQNCQILARKGLPNQKNTITAQGRKDPTEPTGFSIQFSNISAEPDVLASV--NSTPTYLGRPWKEYSRTVFMQSYMGSAIRPEGWLEWNVSFALDTLVYGEYMNYGPGAALSSRVKWPGFHAINDTAQANNFTVAQFILGNSWLPSTGVKYTAGLAL 526
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: M1BB14 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 713.375 bits (1840), Expect = 0.000e+0
Identity = 351/520 (67.50%), Postives = 410/520 (78.85%), Query Frame = 0
 
Query:   85 SYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE----FPEWVKASDRKKLLQVN----GVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            SY  D   +Q +CL +P +EFL S+K+TID+V+QVTS+VS+F+  F DFRLSNAISDCLDLL+LS DEL  TL  +QNP   ++NSTGNLSADLK +LS AL NQDTC EGFDGT G VK+LVA  L +++SLVR IL  V+    +       + RRG    +  +RKL    E    FP W+K  DR+ LLQ +    GVV D+VV  +G GNFT + DAI A P  ST R VIY+KKG+YKEYVEISKKKWN+M++GDG+DVTIISGN +FI GWTTYRSATF VKG+GFIARD+TFENTAGPE HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYR+C ITGTVDFIFGD  A+FQ CQILARKGL  QKNTITAQGRKE AETTGF+IQFSNIS EP+++++   NST TYLGRPWK YSRTV+MQSY+SN IRP+GWLEWN   +LDTLYY EY NYGPGAGLG RV WPG+ +LN +S+AN FTV+QFLLGNSWLP TGVKYTAGL V
Sbjct:   23 SYAVDEDFFQSECLQVPTSEFLGSVKSTIDIVRQVTSLVSKFASFFGDFRLSNAISDCLDLLDLSADELTWTLTASQNPK-GKNNSTGNLSADLKNYLSGALINQDTCTEGFDGTNGLVKNLVAGSLNQVTSLVRDILLMVRPVPKKNVFPSEANARRGKPWQFSRERKLTSNEEDNNSFPSWIKRKDRR-LLQASSPTKGVVTDVVVAHDGTGNFTRIKDAIDAAPQLSTKRFVIYIKKGIYKEYVEISKKKWNIMMIGDGIDVTIISGNRNFIDGWTTYRSATFAVKGQGFIARDITFENTAGPEKHQAVAFRSDSDLSVLFRCAVRGYQDTLYAHSMRQFYRDCTITGTVDFIFGDGTAIFQNCQILARKGLSQQKNTITAQGRKEIAETTGFTIQFSNISGEPDLLASL--NSTYTYLGRPWKTYSRTVIMQSYMSNVIRPQGWLEWNGNISLDTLYYAEYQNYGPGAGLGGRVNWPGFHLLNDSSQANNFTVSQFLLGNSWLPPTGVKYTAGLAV 538
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1U8F5L9 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 711.835 bits (1836), Expect = 0.000e+0
Identity = 349/545 (64.04%), Postives = 421/545 (77.25%), Query Frame = 0
 
Query:   59 IVAVCLPVPVSSEAAEDSTAALLKSCSYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE----FPEWVKASDRKKLLQV---NGVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            +V +C+  P+ +  AED                 +Q +CL +P +EF+ S+K+TID ++QVTSIVS+F+  F DFRL NAISDCLDLLELS DEL  TL  +QNP   ++NSTGNLSADL+ +LS AL NQDTC+EGFDGT G VK+LVA  L +++ LVR IL  V+ P  + +   + + RRG+   W  +RKL   ++    +P W+K   R+ LLQ     GVVAD VV ++G GNFT + DAI+A P  ST + VIY+K+GVY EYVEISKKKWN+M++GDG+DVT+ISGN SFI GWTTYRSATF VKG+GFIARDMTFENTAGPE HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYR+C ITGTVDFIFGD+ A+FQ CQILARKGLP QKNTITAQGRKE AETTGFSIQFSNIS E +++++   NST TYLGRPWK YSRTV+MQSY+S+ IRP+GWLEWN   +LDTLYY EY NYGPGAG G R+KWPGY +LN +S+AN FTVAQF+LGNSWLP TG+KYTAGL V
Sbjct:   16 LVVLCVSCPLVNCGAED----------------FFQSECLQVPISEFVGSVKSTIDTIRQVTSIVSKFANFFSDFRLFNAISDCLDLLELSADELTWTLTASQNPK-GKNNSTGNLSADLRNYLSGALINQDTCIEGFDGTNGLVKNLVAGSLNQVTLLVRDILYMVR-PAPKNAPQGQANDRRGNPWQWSRERKLTSNKQDSHSYPSWLKRKYRRWLLQAAPTKGVVADAVVALDGTGNFTRIKDAIEAAPQLSTKKFVIYIKRGVYNEYVEISKKKWNIMMIGDGIDVTVISGNRSFIDGWTTYRSATFAVKGQGFIARDMTFENTAGPEKHQAVAFRSDSDLSVLFRCAVRGYQDTLYAHSMRQFYRDCIITGTVDFIFGDSTAIFQNCQILARKGLPQQKNTITAQGRKETAETTGFSIQFSNISGEQDLLASL--NSTFTYLGRPWKTYSRTVIMQSYMSSVIRPQGWLEWNGNVSLDTLYYAEYENYGPGAGFGARIKWPGYHLLNDSSQANNFTVAQFILGNSWLPPTGIKYTAGLAV 540
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2G2Y6E1 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 711.835 bits (1836), Expect = 0.000e+0
Identity = 349/545 (64.04%), Postives = 421/545 (77.25%), Query Frame = 0
 
Query:   59 IVAVCLPVPVSSEAAEDSTAALLKSCSYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE----FPEWVKASDRKKLLQV---NGVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            +V +C+  P+ +  AED                 +Q +CL +P +EF+ S+K+TID ++QVTSIVS+F+  F DFRL NAISDCLDLLELS DEL  TL  +QNP   ++NSTGNLSADL+ +LS AL NQDTC+EGFDGT G VK+LVA  L +++ LVR IL  V+ P  + +   + + RRG+   W  +RKL   ++    +P W+K   R+ LLQ     GVVAD VV ++G GNFT + DAI+A P  ST + VIY+K+GVY EYVEISKKKWN+M++GDG+DVT+ISGN SFI GWTTYRSATF VKG+GFIARDMTFENTAGPE HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYR+C ITGTVDFIFGD+ A+FQ CQILARKGLP QKNTITAQGRKE AETTGFSIQFSNIS E +++++   NST TYLGRPWK YSRTV+MQSY+S+ IRP+GWLEWN   +LDTLYY EY NYGPGAG G R+KWPGY +LN +S+AN FTVAQF+LGNSWLP TG+KYTAGL V
Sbjct:   16 LVVLCVSCPLVNCGAED----------------FFQSECLQVPISEFVGSVKSTIDTIRQVTSIVSKFANFFSDFRLFNAISDCLDLLELSADELTWTLTASQNPK-GKNNSTGNLSADLRNYLSGALINQDTCIEGFDGTNGLVKNLVAGSLNQVTLLVRDILYMVR-PAPKNAPQGQANDRRGNPWQWSRERKLTSNKQDSHSYPSWLKRKYRRWLLQAAPTKGVVADAVVALDGTGNFTRIKDAIEAAPQLSTKKFVIYIKRGVYNEYVEISKKKWNIMMIGDGIDVTVISGNRSFIDGWTTYRSATFAVKGQGFIARDMTFENTAGPEKHQAVAFRSDSDLSVLFRCAVRGYQDTLYAHSMRQFYRDCIITGTVDFIFGDSTAIFQNCQILARKGLPQQKNTITAQGRKETAETTGFSIQFSNISGEQDLLASL--NSTFTYLGRPWKTYSRTVIMQSYMSSVIRPQGWLEWNGNVSLDTLYYAEYENYGPGAGFGARIKWPGYHLLNDSSQANNFTVAQFILGNSWLPPTGIKYTAGLAV 540
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5AY66 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 711.064 bits (1834), Expect = 0.000e+0
Identity = 352/516 (68.22%), Postives = 413/516 (80.04%), Query Frame = 0
 
Query:   84 CSYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGREFPEWVKASDRKKLLQVNG---VVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            CS G D+   + +CL +P +EF SSLK TIDVV+QVTSIV++F+  F DFRLSNAISDCLDLL+LS++EL  TL  +QNP N +DNSTGNLSADL+TWLSAAL NQDTC+EGF+G+   VK+LVA  L++++SLV  IL  V+   +  S   +     G   G    RKL   + FP WV  +DRK LLQ NG   V AD VV  +G GNFT + DAI A P+YST R VIY+KKG+Y+EYVEISKKKWN+M++G+GMDVT+ISGN SFI GWTTY SATF VKG+GF+ARD+TFENTAGP+ HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYREC ITGTVDFIFGD   VFQ CQILARKGLPNQKNTITAQGRK+ +E TGFSIQFSNIS EP+++++   NST TYLGRPWKLYSRTV MQSY+ +AIRPEGWLEWN  FALDTL+YGEY NYGPG+GL +R KWPGY  +N +S+AN FTVAQF+LGNSWLP TGVKYTAGL V
Sbjct:   22 CSSGSDF--LELECLKVPTSEFTSSLKNTIDVVRQVTSIVAKFANAFGDFRLSNAISDCLDLLDLSSEELSWTLSASQNP-NGKDNSTGNLSADLRTWLSAALINQDTCMEGFEGSNSIVKNLVAGSLDQVTSLVHDILTMVRQVPNTNS---KGGRSGGGNGGGHSVRKLKSNKHFPYWVGLNDRK-LLQANGNVGVSADAVVAADGTGNFTRIMDAISAAPEYSTRRYVIYIKKGLYREYVEISKKKWNLMMIGEGMDVTVISGNRSFIDGWTTYHSATFAVKGQGFVARDITFENTAGPQKHQAVAFRSDSDLSVLFRCAIRGYQDTLYAHSMRQFYRECQITGTVDFIFGDGTVVFQNCQILARKGLPNQKNTITAQGRKDPSEPTGFSIQFSNISAEPDLLASV--NSTPTYLGRPWKLYSRTVFMQSYMGSAIRPEGWLEWNGNFALDTLFYGEYTNYGPGSGLSSRAKWPGYHAMNDSSQANNFTVAQFILGNSWLPPTGVKYTAGLGV 528
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A3Q7H5H2 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 707.983 bits (1826), Expect = 0.000e+0
Identity = 345/519 (66.47%), Postives = 409/519 (78.81%), Query Frame = 0
 
Query:   85 SYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE----FPEWVKASDRKKLLQVN---GVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            SY  D   +Q +CL +P +EFL S+K+TI++V++VTS+VS+F+  F DFRLSNAISDCLDLL+LS DEL  TL  TQNP   ++NSTGNLSADLK +LS AL NQDTC+EGF+GT G VK+LVA  L ++SSLVR IL  V+    +       + +RG    +  +RKL    E    FP W+K  DR+ L   N   GV+ D+VV ++G GNFT + DAI A P  ST R VIY+KKG+YKEYVEISKKKWN+M++GDG+DVTIISGN +FI GWTTYRSATF VKG+GFIARD+TFENT+GPE HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYR+C ITGTVDFIFGD  A+FQ CQILARKGL  QKNTITAQGRKE AETTGF+IQF NIS EP+++++   NST TYLGRPWK YSRTV+MQSY+SN IRP+GWLEWN   +LDTLYY EY NYGPGAGLG RV WPG+ +LN +S+A+ FTVAQFLLGNSWLP TGVKYTAGL V
Sbjct:   26 SYAVDDNFFQSECLQVPTSEFLGSVKSTINIVREVTSLVSKFASFFGDFRLSNAISDCLDLLDLSADELTWTLTATQNPK-GKNNSTGNLSADLKNYLSGALINQDTCIEGFEGTNGLVKNLVAGNLNQVSSLVRNILLMVRPVSRKNVFPSEANAKRGKPWQFSRERKLTSNEEDHNLFPSWIKRKDRRLLQASNSTKGVITDVVVALDGTGNFTRIKDAIDAAPQLSTKRFVIYIKKGIYKEYVEISKKKWNIMMIGDGIDVTIISGNRNFIDGWTTYRSATFAVKGQGFIARDITFENTSGPEKHQAVAFRSDSDLSVLFRCAVRGYQDTLYAHSMRQFYRDCTITGTVDFIFGDGTAIFQNCQILARKGLSQQKNTITAQGRKEIAETTGFTIQFCNISGEPDLLTSL--NSTYTYLGRPWKTYSRTVIMQSYMSNVIRPQGWLEWNGNTSLDTLYYAEYQNYGPGAGLGGRVNWPGFHLLNDSSQASNFTVAQFLLGNSWLPPTGVKYTAGLAV 541
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2G9H803 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 707.983 bits (1826), Expect = 0.000e+0
Identity = 356/517 (68.86%), Postives = 416/517 (80.46%), Query Frame = 0
 
Query:   84 CSYGD----DYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGREFPEWVKASDRKKLLQVNGVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            CS+G     D+ +   + L +PA+EF+ S+K+TID+++QV SIVSEF   F DFRLSNA+SDCLDL++LS D+L  TL  +QN  N +DNSTGN+ AD+KTWLS AL NQDTC EGFDGT G +K+LVA  L++++SL+  IL+NVK    +  G        G   G    RKL+   +FP WVK+ DR  L   NGVVAD VV  +G GNF T+ DAI A P+YST R VIY+KKGVYKEYVEISKKKWN+ML+GDGMDVT+ISGNH+FI GWTTYRSATF VKG+GFIARD+TFENTAGPE HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHSQRQFYREC ITGTVDFIFGD A VFQ CQI ARKGLPNQKNTITAQGRKE+ E TGFS+QF NISVEP+V+     NST TYLGRPWKLYSRTV+MQSYISNAIRPEGWLEWN  FALDTL+YGEY NYGPG+ LG RVKWPGYRV N+++EA+ FTV+QF++GN+WLPSTGV+YTAGL V
Sbjct:   18 CSFGSGKSSDF-VAPAEYLDVPASEFVGSVKSTIDIIRQVISIVSEFGGVFGDFRLSNAVSDCLDLMDLSVDQLSWTLSASQN-SNGKDNSTGNVGADMKTWLSGALINQDTCKEGFDGTNGILKNLVAGSLDQVTSLIYDILSNVKPIPSQGGGKGSGGGSGGGGGGRSGGRKLVADDKFPHWVKSHDRNLLQAPNGVVADAVVAADGTGNFATIKDAILAAPEYSTKRYVIYIKKGVYKEYVEISKKKWNIMLIGDGMDVTVISGNHNFIDGWTTYRSATFAVKGQGFIARDITFENTAGPEKHQAVAFRSDSDLSVLFRCAIRGYQDTLYAHSQRQFYRECIITGTVDFIFGDGAVVFQNCQIQARKGLPNQKNTITAQGRKESVENTGFSVQFCNISVEPDVL-----NSTQTYLGRPWKLYSRTVIMQSYISNAIRPEGWLEWNGDFALDTLFYGEYSNYGPGSVLGARVKWPGYRVFNSSAEASNFTVSQFIIGNTWLPSTGVRYTAGLQV 527
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A830BBJ6 ((Pectinesterase/pectinesterase inhibitor ppe8b {ECO:0000313|EMBL:GFP82144.1}))

HSP 1 Score: 707.983 bits (1826), Expect = 0.000e+0
Identity = 346/518 (66.80%), Postives = 413/518 (79.73%), Query Frame = 0
 
Query:   80 LLKSCSYGDDYKIYQP-DCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILANVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGREFPEWVKASDRKKLLQVNGVVADLVVDVNGYGNFTTLSDAIKAVPDYSTNRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            LL  C +G     + P + L IPA+EF+ S+++TID+V+QV S+VS+F+    DFRLSNA+SDCLDL++LS D+L  T+  +QNP N +DNSTGN+ AD+KTWLS AL+NQDTC EGF+GT G VK+LVA  L++I+SLV  IL+NVK+   +                    RKLIGG   P W+++ DR  L   NG +AD+VV  +G GNFT++ +AI A P++ST R VIY+K+GVYKEYVEISKKKWNVM++GDG++ T+ISGN +FI GWTTYRSATF VKG+GFIARD+TFENTAGPE HQAVAFRSDSDLSVL+RCA RGYQDTLYAHSQRQFYREC I+GTVDFIFGD A VFQ C+ILAR+GLPNQKNTITAQGRKE  E TGFSIQF NISVEPNVV     NST TYLGRPWKLYSRTV+MQSYISNAIRPEGWLEWN  FALDTL+YGEY NYGPGAGLG RVKWPGYRV N++++A+ FTV+QF+ GN WLPSTGV++TAGL V
Sbjct:    5 LLLICCFGCGKSDFDPSESLKIPASEFVGSVRSTIDIVRQVISMVSQFAGALGDFRLSNAVSDCLDLMDLSVDQLSWTISASQNP-NGKDNSTGNIGADMKTWLSGALTNQDTCNEGFEGTNGIVKNLVAGSLDQITSLVYDILSNVKTTPTKGGAKGSGGRS-------GGGRKLIGG---PGWLRSRDRNLLQAANGAMADVVVAADGTGNFTSIEEAIGAAPEHSTKRFVIYIKRGVYKEYVEISKKKWNVMMIGDGINATVISGNRNFIDGWTTYRSATFAVKGQGFIARDITFENTAGPEKHQAVAFRSDSDLSVLFRCAFRGYQDTLYAHSQRQFYRECQISGTVDFIFGDGAVVFQNCEILARQGLPNQKNTITAQGRKEPVENTGFSIQFCNISVEPNVV-----NSTQTYLGRPWKLYSRTVIMQSYISNAIRPEGWLEWNGDFALDTLFYGEYSNYGPGAGLGTRVKWPGYRVFNSSAQASNFTVSQFITGNEWLPSTGVRFTAGLGV 506
[Close]
BLAST of DCAR_028537 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6Q5B5 (Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589} (3.1.1.11 {ECO:0000256|ARBA:ARBA00013229, ECO:0000256|RuleBase:RU000589}))

HSP 1 Score: 700.664 bits (1807), Expect = 0.000e+0
Identity = 349/525 (66.48%), Postives = 412/525 (78.48%), Query Frame = 0
 
Query:   78 AALLKSCSYGDDYKIYQPDCLTIPATEFLSSLKTTIDVVQQVTSIVSEFSRGFLDFRLSNAISDCLDLLELSTDELVSTLLLTQNPHNSQDNSTGNLSADLKTWLSAALSNQDTCVEGFDGTRGFVKSLVASGLEEISSLVRVILA----NVKSPHDRKSGSRRRHHRRGDMPGWMHKRKLIGGRE-FPEWVKASDRKKLLQVNGVVADLVVDVNGYGNFTTLSDAIKAVPDYST-NRTVIYVKKGVYKEYVEISKKKWNVMLVGDGMDVTIISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGYQDTLYAHSQRQFYRECHITGTVDFIFGDAAAVFQKCQILARKGLPNQKNTITAQGRKEAAETTGFSIQFSNISVEPNVVSNSTGNSTLTYLGRPWKLYSRTVVMQSYISNAIRPEGWLEWNTTFALDTLYYGEYMNYGPGAGLGNRVKWPGYRVLNTTSEANAFTVAQFLLGNSWLPSTGVKYTAGLVV 596
            A  L SC  G D  +   +CL +P +EF  ++K+T+DVVQQV S+ S+F+  F DFRLSNAI+DCLDLL+LS+++L  TL  +QNP N ++NSTGNLSADL+TWLS AL NQDTC EG DGT   VK LV+  L +++SLV+ IL     N+  P+ + S                   KLI   + FP W+K  DR+ L+Q NG+  D VV  +G GNFT + DAI A PDY+  +R VIYVKKGVY EYVEISKKKWN++++GDGMDVT+ISGN SFI GWTTYRSATF VKG+GFIARDMTF+NTAGP+ HQAVAFRSDSDLSVL+RCA+RGYQDTLYAHS RQFYRECHITGTVDFIFGD +A+FQKCQILARKGLPNQKNTITAQGRK   E TGFSIQFSNIS EP+V+++   NSTLTYLGRPWKL SRTV MQSY+SNAIRPEGWLEWN  FALDTL+YGEYMNYGPGAGLG+RVKWPG+  +N +++AN FTV+QF+LGNSWLPSTGVKY AGL V
Sbjct:   16 AVCLGSC--GSD--LVDSECLKVPISEFGGAVKSTMDVVQQVISLFSKFASTFGDFRLSNAIADCLDLLDLSSEQLSRTLSASQNP-NGKNNSTGNLSADLRTWLSGALINQDTCTEGLDGTNSIVKVLVSGSLNQVTSLVQEILTMVNQNIPDPNAKGSRGGGGGSY-------PPGSKLIKTDDHFPYWLKPKDRR-LIQANGISVDAVVATDGTGNFTKIMDAISAAPDYNNAHRFVIYVKKGVYNEYVEISKKKWNLLMIGDGMDVTVISGNRSFIDGWTTYRSATFAVKGQGFIARDMTFQNTAGPQKHQAVAFRSDSDLSVLFRCAIRGYQDTLYAHSMRQFYRECHITGTVDFIFGDGSAIFQKCQILARKGLPNQKNTITAQGRKSPNEPTGFSIQFSNISAEPDVLASL--NSTLTYLGRPWKLCSRTVFMQSYMSNAIRPEGWLEWNRDFALDTLFYGEYMNYGPGAGLGSRVKWPGFHAINDSAQANNFTVSQFILGNSWLPSTGVKYIAGLAV 525
Match NameStatsDescription
A0A6A4L156E-Value: 0.000e+0, PID: 68.69Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A5B6YM53E-Value: 0.000e+0, PID: 68.80Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
M1BB14E-Value: 0.000e+0, PID: 67.50Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A1U8F5L9E-Value: 0.000e+0, PID: 64.04Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A2G2Y6E1E-Value: 0.000e+0, PID: 64.04Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A5J5AY66E-Value: 0.000e+0, PID: 68.22Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A3Q7H5H2E-Value: 0.000e+0, PID: 66.47Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A2G9H803E-Value: 0.000e+0, PID: 68.86Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]
A0A830BBJ6E-Value: 0.000e+0, PID: 66.80(Pectinesterase/pectinesterase inhibitor ppe8b {EC... [more]
A0A2R6Q5B5E-Value: 0.000e+0, PID: 66.48Pectinesterase {ECO:0000256|ARBA:ARBA00013229, ECO... [more]

Pages

back to top
Analysis: 
NameDescription
Carrot Genome Assembly DCARv2

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search
DCAR peptide blastp to SwissProt and TrEMBL without DCAR

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
DCAR V1.0 Gene Prediction

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.