Resource Type:
Polypeptide
Name:
DCAR_027380
Identifier:
DCAR_027380.mRNA-protein
Sequence:
MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANA
SNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIY
LSSAFLEFLGWLILTVQAQSASLRPAECEANVPCRRVGGAKAAMLFAGLY
LAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIA
VTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTT
ILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKA
PSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNH
YHLERFYWLMCALSILNFLHYLFWANRYKYKALNKG*
Sequence Length:
387
Sequence Checksum:
6d3d2c1610ad345ab12296a101712e9e
View location in JBrowse:
Organism:
Relationship:
| Relationships |
|---|
| The polypeptide, DCAR_027380, derives from mRNA, DCAR_027380. |
Blast Results:
The following BLAST results are available for this feature:
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7G2E7S8 (((thale cress) hypothetical protein {ECO:0000313|EMBL:CAD5316780.1}))
HSP 1 Score: 446.047 bits (1146), Expect = 4.456e-153
Identity = 248/449 (55.23%), Postives = 303/449 (67.48%), Query Frame = 0
Match: A0A7G2E7S8 (((thale cress) hypothetical protein {ECO:0000313|EMBL:CAD5316780.1}))
HSP 1 Score: 446.047 bits (1146), Expect = 4.456e-153
Identity = 248/449 (55.23%), Postives = 303/449 (67.48%), Query Frame = 0
Query: 1 MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEANVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMK----MENQ----------------QMT-------KKSKAPSSMRSLATSLSWASLA---------------MGYFLSTVIVSIVNSAT-------------------------GSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
MEVE +++RW+GY DWRN+ A++GRHGGMLAASFVLVVEILENLA+LANASNLV+YL E+MH SP SAN VTNFMGTAFLLALLGGFLSDAF +T+ I+L SA +EFLG +ILT+QA++ SL P C++ C V G+KAAMLF GLYL ALG+GGIKGSLA+HGAEQFDE TP+GRKQRSTFFNYFVF LA GAL+AVT VVW+EDN+GW WGFG+ST IF+SI +FL+GS+FYRNKIPCGSPLTTILKVL A+++ C + S +NA+ASMS SP+ K +E+Q Q+T K S S +L + W +L + YF + S+ + AT GS+ N PWL G ++N Y L+ FYWLMC LS NFLHYLFWA RYKY++
Sbjct: 1 MEVEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPT-CEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCC--SSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 446
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A835IYC5 ((Uncharacterized protein {ECO:0000313|EMBL:KAF9623803.1}))
HSP 1 Score: 436.032 bits (1120), Expect = 3.643e-151
Identity = 221/315 (70.16%), Postives = 251/315 (79.68%), Query Frame = 0
Match: A0A835IYC5 ((Uncharacterized protein {ECO:0000313|EMBL:KAF9623803.1}))
HSP 1 Score: 436.032 bits (1120), Expect = 3.643e-151
Identity = 221/315 (70.16%), Postives = 251/315 (79.68%), Query Frame = 0
Query: 1 MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECE---ANVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKA--PSSMRSLATS 310
ME HQL RW+GYV+WRNKPALRG HGGMLAASFVLVVEILENLAFLANASNLV+YLS+ MHFSP SAN VTNFMGTAFLLALLGGF SDAFL TY IYL SA +EFLG +ILT+QA SL+P C+ + C+ G KA MLF GLYL ALG+GGIKGSLATHGAEQ DE TPQGRKQRSTFFNYFVF LA GALIAVTLVVW+EDN GW WGFGIST TIFLSIP+FL GS YRNK+P GSPLTTI KVLAA+ LNTC ++RS +NA+ASM+ SPA+ ++E ++ K+K P+ + A+S
Sbjct: 1 MEEGHQLERWEGYVNWRNKPALRGHHGGMLAASFVLVVEILENLAFLANASNLVLYLSKFMHFSPSYSANHVTNFMGTAFLLALLGGFFSDAFLTTYHIYLISAVIEFLGLVILTLQAHLPSLKPPTCKPVTTGISCQEATGPKAMMLFGGLYLIALGVGGIKGSLATHGAEQLDESTPQGRKQRSTFFNYFVFCLAFGALIAVTLVVWMEDNMGWEWGFGISTATIFLSIPIFLLGSATYRNKVPTGSPLTTIAKVLAAAMLNTC--ISRSSSNAVASMATSPAY----QIECTEVEDKAKEEIPTKTKRRASS 309
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6ZKJ5 ((Putative Nitrate transporter 1:2 {ECO:0000313|EMBL:MPA44950.1}) (Fragment))
HSP 1 Score: 438.728 bits (1127), Expect = 2.505e-148
Identity = 221/303 (72.94%), Postives = 252/303 (83.17%), Query Frame = 0
HSP 2 Score: 152.14 bits (383), Expect = 2.461e-38
Identity = 70/85 (82.35%), Postives = 78/85 (91.76%), Query Frame = 0
Match: A0A5B6ZKJ5 ((Putative Nitrate transporter 1:2 {ECO:0000313|EMBL:MPA44950.1}) (Fragment))
HSP 1 Score: 438.728 bits (1127), Expect = 2.505e-148
Identity = 221/303 (72.94%), Postives = 252/303 (83.17%), Query Frame = 0
Query: 2 EVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEA---NVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAP 301
EVEH+L W+GYVDWRN+PALRGRHGGMLAASFVLVVE+LENLA+LANASNLV YLS++MHFSP SANSVTNFMGT+FLLALLGGFLSDAFL TY IYL SA +EFLG +ILTVQA S SL+P +C+ N+PC+ V G KAAMLF GLYL ALG+GGIKGSL HGAEQFDE+ PQGRKQRSTFFNYFVF L+ G LIAVTLVVWIEDN+GW WGFGIST TI SIP+FLAGS FYRNKIPCGSPLTTILKVL A+ LN C + +SP+NAI SM+ SP+ T + E+++ K+ P
Sbjct: 1 EVEHKLNIWEGYVDWRNRPALRGRHGGMLAASFVLVVEVLENLAYLANASNLVRYLSKYMHFSPSKSANSVTNFMGTSFLLALLGGFLSDAFLTTYHIYLISAVVEFLGLVILTVQAHSPSLKPPKCDPATPNIPCQEVHGGKAAMLFMGLYLVALGVGGIKGSLPAHGAEQFDENNPQGRKQRSTFFNYFVFCLSCGGLIAVTLVVWIEDNKGWEWGFGISTLTILSSIPIFLAGSSFYRNKIPCGSPLTTILKVLVAATLNNC--ITKSPSNAITSMTTSPSPPTPNRKEDEEANAKAMEP 301
HSP 2 Score: 152.14 bits (383), Expect = 2.461e-38
Identity = 70/85 (82.35%), Postives = 78/85 (91.76%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APS+MRSLATSLSWASLAMGY+LSTVIVSIVNS T + +KPWLSG NLNHY LERFYWLMCALS LNFLHYLFWANRYKY++
Sbjct: 490 TEAPSNMRSLATSLSWASLAMGYYLSTVIVSIVNSVTDKSNHKPWLSGSNLNHYQLERFYWLMCALSGLNFLHYLFWANRYKYRS 574
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2I4FHK4 ((protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|RefSeq:XP_018831139.1}))
HSP 1 Score: 435.261 bits (1118), Expect = 7.284e-147
Identity = 222/315 (70.48%), Postives = 257/315 (81.59%), Query Frame = 0
HSP 2 Score: 141.739 bits (356), Expect = 1.379e-34
Identity = 64/85 (75.29%), Postives = 76/85 (89.41%), Query Frame = 0
Match: A0A2I4FHK4 ((protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|RefSeq:XP_018831139.1}))
HSP 1 Score: 435.261 bits (1118), Expect = 7.284e-147
Identity = 222/315 (70.48%), Postives = 257/315 (81.59%), Query Frame = 0
Query: 1 MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAEC---EANVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVA------VARSPNNAIASMS---ASPAFGTSMKMENQQMTKKSKAPSS 303
ME E QL RW+GYVDWR +PALRGRHGGMLAASFVLVVEILENLAFLANASNLV+YLSE+MHFSP SAN+VTNFMGTAFLLALLGGFLSDAF TY IYL SA +EFLG ++LTVQA+S SL+P+ C ++PC+ G +AAMLF GLYL ALG+GGIKGSL HGAEQFDEDTPQGRKQRSTFFNYFVF L+ G L+AVTLVVWIEDN+GW WGFGIST +I LSIPLFLAGS YRNKIPCGSPLTTILKVL A+ LN+C++ ++RSP+NA+ASM+ +SP F + EN + + + AP+
Sbjct: 1 MEEEQQLTRWEGYVDWRRRPALRGRHGGMLAASFVLVVEILENLAFLANASNLVLYLSEYMHFSPTKSANNVTNFMGTAFLLALLGGFLSDAFFTTYHIYLISAVIEFLGLVVLTVQARSPSLKPSACGPANLDIPCQEAHGGRAAMLFLGLYLVALGVGGIKGSLPAHGAEQFDEDTPQGRKQRSTFFNYFVFCLSCGGLVAVTLVVWIEDNKGWEWGFGISTISILLSIPLFLAGSATYRNKIPCGSPLTTILKVLVAATLNSCISRSPKGCISRSPSNAVASMATSPSSPTFTSKEAEENAKARESTDAPTE 315
HSP 2 Score: 141.739 bits (356), Expect = 1.379e-34
Identity = 64/85 (75.29%), Postives = 76/85 (89.41%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATS SWASLAMGY+LS+VIVSIVN+ TG +K++PWLSG NLNH+ LERFYWLMC LS LNFL+YL WA RYKY++
Sbjct: 498 TEAPSSMRSLATSFSWASLAMGYYLSSVIVSIVNNVTGISKHRPWLSGSNLNHFQLERFYWLMCVLSALNFLNYLLWAIRYKYRS 582
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6V692 ((protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|RefSeq:XP_027098383.1}))
HSP 1 Score: 434.491 bits (1116), Expect = 1.157e-146
Identity = 213/305 (69.84%), Postives = 250/305 (81.97%), Query Frame = 0
HSP 2 Score: 153.295 bits (386), Expect = 8.892e-39
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 0
Match: A0A6P6V692 ((protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|RefSeq:XP_027098383.1}))
HSP 1 Score: 434.491 bits (1116), Expect = 1.157e-146
Identity = 213/305 (69.84%), Postives = 250/305 (81.97%), Query Frame = 0
Query: 1 MEVEHQL-ARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEA---NVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAP 301
MEVE+Q W+GYV WRN+PAL+GRHGGM+AASFVLV E+LENLA+LANASNLV+Y SE+MHFSP +SANSVTNFMGTAFLLAL+GGFLSDAF TYFIYL SA +EFLG +ILTVQA S SL+P +C+ ++PC++V GAKAAMLF GLYL ALG+GGIKGSL HGAEQFDED+P+GRKQRSTFFNYFVF L+ GALIAVTLVVW+EDN+GW WGFGISTF I LSIP+FL GS +YRNKIP GSPLTTI+KVL A+ LN+ +R NNAIASM+ SP + + E + K+ P
Sbjct: 1 MEVENQQPGSWEGYVSWRNRPALKGRHGGMVAASFVLVAEVLENLAYLANASNLVLYFSEYMHFSPSNSANSVTNFMGTAFLLALVGGFLSDAFFTTYFIYLISALIEFLGLVILTVQAHSPSLKPPKCDPATPSIPCQKVHGAKAAMLFIGLYLVALGVGGIKGSLPAHGAEQFDEDSPKGRKQRSTFFNYFVFCLSCGALIAVTLVVWVEDNKGWQWGFGISTFAILLSIPIFLLGSTYYRNKIPNGSPLTTIVKVLLAALLNSF--KSRGSNNAIASMATSPTPTPASEEEGGNIKSKTVEP 303
HSP 2 Score: 153.295 bits (386), Expect = 8.892e-39
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATSLSWASLAMGY+LSTVIVSIVNSATG++ +KPWLSG NLNHYHL+RFYW+MC LS LNFLHYLFWA RYKY++
Sbjct: 493 TEAPSSMRSLATSLSWASLAMGYYLSTVIVSIVNSATGNSDHKPWLSGSNLNHYHLDRFYWVMCVLSGLNFLHYLFWAMRYKYRS 577
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6UXC9 ((protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO:0000313|RefSeq:XP_027095419.1}))
HSP 1 Score: 434.106 bits (1115), Expect = 1.603e-146
Identity = 216/323 (66.87%), Postives = 259/323 (80.19%), Query Frame = 0
HSP 2 Score: 153.295 bits (386), Expect = 9.928e-39
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 0
Match: A0A6P6UXC9 ((protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO:0000313|RefSeq:XP_027095419.1}))
HSP 1 Score: 434.106 bits (1115), Expect = 1.603e-146
Identity = 216/323 (66.87%), Postives = 259/323 (80.19%), Query Frame = 0
Query: 1 MEVEHQL-ARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEA---NVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAPSSMRSLATSLSWASLAMG 319
MEVE+Q W+GYV WRN+PAL+GRHGGM+AASFVLV E+LENLA+LANASNLV+Y SE+MHFSP +SANSVTNFMGTAFLLAL+GGFLSDAF TYFIYL A +EFLG +ILTVQA S SL+P +C+ ++PC++V GAKAAMLF GLYL ALG+GGIKGSL HGAEQFDED+P+GRKQRSTFFNYFVF L+ GALIAVTLVVW+EDN+GW WGFGISTF I LSIP+FL GS +YRNKIP GSPLTTI+KVL A+ LN+ +R NNAIASM+ SP + + E + K+ P+ + + SL + + A+G
Sbjct: 1 MEVENQQPGSWEGYVSWRNRPALKGRHGGMVAASFVLVAEVLENLAYLANASNLVLYFSEYMHFSPSNSANSVTNFMGTAFLLALVGGFLSDAFFTTYFIYLIGALIEFLGLVILTVQAHSPSLKPPKCDPATPSIPCQKVHGAKAAMLFIGLYLVALGVGGIKGSLPAHGAEQFDEDSPKGRKQRSTFFNYFVFCLSCGALIAVTLVVWVEDNKGWQWGFGISTFAILLSIPIFLLGSTYYRNKIPNGSPLTTIVKVLLAALLNSF--KSRGSNNAIASMATSPTPTPASEEEGGNIKSKTVEPTQ-ETPSKSLQFLNRAVG 320
HSP 2 Score: 153.295 bits (386), Expect = 9.928e-39
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATSLSWASLAMGY+LSTVIVSIVNSATG++ +KPWLSG NLNHYHL+RFYW+MC LS LNFLHYLFWA RYKY++
Sbjct: 493 TEAPSSMRSLATSLSWASLAMGYYLSTVIVSIVNSATGNSNHKPWLSGSNLNHYHLDRFYWVMCVLSGLNFLHYLFWAMRYKYRS 577
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P5XDH3 ((protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO:0000313|RefSeq:XP_022725712.1}))
HSP 1 Score: 439.113 bits (1128), Expect = 2.084e-146
Identity = 210/299 (70.23%), Postives = 241/299 (80.60%), Query Frame = 0
HSP 2 Score: 140.969 bits (354), Expect = 5.223e-34
Identity = 65/85 (76.47%), Postives = 76/85 (89.41%), Query Frame = 0
Match: A0A6P5XDH3 ((protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO:0000313|RefSeq:XP_022725712.1}))
HSP 1 Score: 439.113 bits (1128), Expect = 2.084e-146
Identity = 210/299 (70.23%), Postives = 241/299 (80.60%), Query Frame = 0
Query: 9 RWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEANVP---CRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAPSSM 304
+W+GY DWRN+PALRGRHGGMLAASFVLVVE+LENLA+LANASNLV+YLS+HMH SP SAN+VTNFMGTAFLLALLGG LSDAF TY IYL SA +EFLG +IL +QA++ SL+P EC + P C+ VGG KAAMLF GLYL ALG+GGIKGSL THGAEQFDE TPQGRKQRSTFFNYF+F L+ GALIAVT VVWIEDN+GW WGF IST I LSIP+FL GS FYRNKIP GSPL TI KVL A+ LN+C ++RSP NAIA ++ASP + T E + K+SK P +
Sbjct: 167 KWEGYADWRNRPALRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKHMHLSPSKSANNVTNFMGTAFLLALLGGCLSDAFFTTYHIYLISALIEFLGLIILAMQARTPSLKPPECNPSTPSNPCQEVGGGKAAMLFIGLYLVALGVGGIKGSLPTHGAEQFDESTPQGRKQRSTFFNYFIFCLSCGALIAVTFVVWIEDNKGWEWGFAISTVAILLSIPIFLVGSTFYRNKIPFGSPLKTISKVLVAAMLNSC--MSRSPRNAIAYLAASPYYPTETSKEAEDNAKQSKQPDQI 463
HSP 2 Score: 140.969 bits (354), Expect = 5.223e-34
Identity = 65/85 (76.47%), Postives = 76/85 (89.41%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATSLSWASLAMGY+LS+VIVSIVN+ T ++ +KPWLSG +NHYHLERFYWLMC LS LNFLHYLF A RYKY++
Sbjct: 649 TEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNNLTDNSGHKPWLSGHTINHYHLERFYWLMCVLSALNFLHYLFRAMRYKYRS 733
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A833U3U8 ((Uncharacterized protein {ECO:0000313|EMBL:KAF5449713.1}) (Fragment))
HSP 1 Score: 434.491 bits (1116), Expect = 1.043e-145
Identity = 222/315 (70.48%), Postives = 257/315 (81.59%), Query Frame = 0
HSP 2 Score: 141.354 bits (355), Expect = 2.584e-34
Identity = 64/85 (75.29%), Postives = 76/85 (89.41%), Query Frame = 0
Match: A0A833U3U8 ((Uncharacterized protein {ECO:0000313|EMBL:KAF5449713.1}) (Fragment))
HSP 1 Score: 434.491 bits (1116), Expect = 1.043e-145
Identity = 222/315 (70.48%), Postives = 257/315 (81.59%), Query Frame = 0
Query: 1 MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAEC---EANVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVA------VARSPNNAIASMS---ASPAFGTSMKMENQQMTKKSKAPSS 303
ME E QL RW+GYVDWR +PALRGRHGGMLAASFVLVVEILENLAFLANASNLV+YLSE+MHFSP SAN+VTNFMGTAFLLALLGGFLSDAF TY IYL SA +EFLG ++LTVQA+S SL+P+ C ++PC+ G +AAMLF GLYL ALG+GGIKGSL HGAEQFDEDTPQGRKQRSTFFNYFVF L+ G L+AVTLVVWIEDN+GW WGFGIST +I LSIPLFLAGS YRNKIPCGSPLTTILKVL A+ LN+C++ ++RSP+NA+ASM+ +SP F + EN + + + AP+
Sbjct: 72 MEEEQQLTRWEGYVDWRRRPALRGRHGGMLAASFVLVVEILENLAFLANASNLVLYLSEYMHFSPTKSANNVTNFMGTAFLLALLGGFLSDAFFTTYHIYLISAVIEFLGLVVLTVQARSPSLKPSACGPANLDIPCQEAHGGRAAMLFLGLYLVALGVGGIKGSLPAHGAEQFDEDTPQGRKQRSTFFNYFVFCLSCGGLVAVTLVVWIEDNKGWEWGFGISTISILLSIPLFLAGSATYRNKIPCGSPLTTILKVLVAATLNSCISRSPKGCISRSPSNAVASMATSPSSPTFTSKEAEENAKARESTDAPTE 386
HSP 2 Score: 141.354 bits (355), Expect = 2.584e-34
Identity = 64/85 (75.29%), Postives = 76/85 (89.41%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATS SWASLAMGY+LS+VIVSIVN+ TG +K++PWLSG NLNH+ LERFYWLMC LS LNFL+YL WA RYKY++
Sbjct: 569 TEAPSSMRSLATSFSWASLAMGYYLSSVIVSIVNNVTGISKHRPWLSGSNLNHFQLERFYWLMCVLSALNFLNYLLWAIRYKYRS 653
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A068TVL7 ((Uncharacterized protein {ECO:0000313|EMBL:CDP00281.1}))
HSP 1 Score: 434.491 bits (1116), Expect = 2.879e-145
Identity = 216/323 (66.87%), Postives = 260/323 (80.50%), Query Frame = 0
HSP 2 Score: 150.984 bits (380), Expect = 1.198e-37
Identity = 69/85 (81.18%), Postives = 79/85 (92.94%), Query Frame = 0
Match: A0A068TVL7 ((Uncharacterized protein {ECO:0000313|EMBL:CDP00281.1}))
HSP 1 Score: 434.491 bits (1116), Expect = 2.879e-145
Identity = 216/323 (66.87%), Postives = 260/323 (80.50%), Query Frame = 0
Query: 1 MEVEHQL-ARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEA---NVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAPSSMRSLATSLSWASLAMG 319
MEVE+Q W+GYV+WRN+PAL+GRHGGM+AASFVLV E+LENLA+LANASNLV+Y SE+MHFSP +SANSVTNFMGTAFLLAL+GGFLSDAF TYFIYL A +EFLG +ILTVQA S SL+P +C+ ++PC++V GAKAAMLF GLYL ALG+GGIKGSL HGAEQFDED+P+GRKQRSTFFNYFVF L+ GALIAVTLVVW+EDN+GW WGFGISTF I LSIP+FL GS +YRNKIP GSPLTTI+KVL A+ LN+ +R NNAIASM+ SP + + E + K+ P+ + + SL + + A+G
Sbjct: 105 MEVENQQPGSWEGYVNWRNRPALKGRHGGMVAASFVLVAEVLENLAYLANASNLVLYFSEYMHFSPSNSANSVTNFMGTAFLLALVGGFLSDAFFTTYFIYLIGALIEFLGLVILTVQAHSPSLKPPKCDPATPSIPCQKVHGAKAAMLFIGLYLVALGVGGIKGSLPAHGAEQFDEDSPKGRKQRSTFFNYFVFCLSCGALIAVTLVVWVEDNKGWQWGFGISTFAILLSIPIFLLGSTYYRNKIPNGSPLTTIVKVLLAALLNS--FKSRGSNNAIASMATSPTPTPASEEEGGNIKSKTVEPTQ-ETPSKSLQFLNRAVG 424
HSP 2 Score: 150.984 bits (380), Expect = 1.198e-37
Identity = 69/85 (81.18%), Postives = 79/85 (92.94%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLMCALSILNFLHYLFWANRYKYKA 382
++APSSMRSLATSLSW SLAMGY+LSTVIVSIVNSATG++ +KPWLSG NLNHYHL+RFYW+MC LS LNFLHYLFWA RYKY++
Sbjct: 597 TEAPSSMRSLATSLSWVSLAMGYYLSTVIVSIVNSATGNSNHKPWLSGSNLNHYHLDRFYWVMCVLSGLNFLHYLFWAMRYKYRS 681
BLAST of DCAR_027380 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5AHX0 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8529472.1}))
HSP 1 Score: 428.713 bits (1101), Expect = 1.051e-144
Identity = 227/321 (70.72%), Postives = 261/321 (81.31%), Query Frame = 0
HSP 2 Score: 102.449 bits (254), Expect = 4.921e-21
Identity = 48/63 (76.19%), Postives = 55/63 (87.30%), Query Frame = 0
Match: A0A5J5AHX0 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8529472.1}))
HSP 1 Score: 428.713 bits (1101), Expect = 1.051e-144
Identity = 227/321 (70.72%), Postives = 261/321 (81.31%), Query Frame = 0
Query: 1 MEVEHQLARWDGYVDWRNKPALRGRHGGMLAASFVLVVEILENLAFLANASNLVMYLSEHMHFSPKDSANSVTNFMGTAFLLALLGGFLSDAFLNTYFIYLSSAFLEFLGWLILTVQAQSASLRPAECEA---NVPCRRVGGAKAAMLFAGLYLAALGIGGIKGSLATHGAEQFDEDTPQGRKQRSTFFNYFVFSLATGALIAVTLVVWIEDNRGWVWGFGISTFTIFLSIPLFLAGSKFYRNKIPCGSPLTTILKVLAASALNTCVAVARSPNNAIASMSASPAFGTSMKMENQQMTKKSKAPSSMRSLATSLSWASLAM 318
ME+EHQ+ W+GYVDWRN+PALRGR GGMLAA FVLVVE+LENLAFLANASNLV+YLSE+MHFSP SANSVTNFMGTAFLLALLGGFLSDAF TY +YL+SA +EFLG +ILTVQA S SL+P +C+ ++PC+ V G KAAMLF GLYL ALG+GGIKGSL HGAEQFDE+T QGRKQRSTFFNYFVFSL+ G LIAVTLVVWIEDN+GW WGFGIST IFLSIP+FLAGS FYRNKIPCGSPLTTI KVL A+ LNTC+ + RSP+NAI SMS SP+ T + E + SK S+ + SL + + A+
Sbjct: 1 MELEHQVNTWEGYVDWRNRPALRGRQGGMLAAFFVLVVEVLENLAFLANASNLVLYLSEYMHFSPSKSANSVTNFMGTAFLLALLGGFLSDAFFTTYQVYLTSAVVEFLGLVILTVQAHSPSLKPPKCDPATPDIPCQEVHGGKAAMLFLGLYLVALGVGGIKGSLPAHGAEQFDENTLQGRKQRSTFFNYFVFSLSCGGLIAVTLVVWIEDNKGWEWGFGISTLAIFLSIPVFLAGSSFYRNKIPCGSPLTTIFKVLLAATLNTCICITRSPSNAITSMSTSPSSPTPTRKEADE--ANSKTMESIETPTKSLKFLNRAV 319
HSP 2 Score: 102.449 bits (254), Expect = 4.921e-21
Identity = 48/63 (76.19%), Postives = 55/63 (87.30%), Query Frame = 0
Query: 298 SKAPSSMRSLATSLSWASLAMGYFLSTVIVSIVNSATGSAKNKPWLSGPNLNHYHLERFYWLM 360
++AP SMRSLATSLS+ASLAMGY+LS+ IVSIVNS TG K+ PWLSG NLN YHLERFYWL+
Sbjct: 494 TEAPISMRSLATSLSFASLAMGYYLSSAIVSIVNSVTGHGKHNPWLSGSNLNRYHLERFYWLI 556
| Match Name | Stats | Description |
|---|---|---|
| A0A7G2E7S8 | E-Value: 4.456e-153, PID: 55.23 | ((thale cress) hypothetical protein {ECO:0000313|E... [more] |
| A0A835IYC5 | E-Value: 3.643e-151, PID: 70.16 | (Uncharacterized protein {ECO:0000313|EMBL:KAF9623... [more] |
| A0A5B6ZKJ5 | E-Value: 2.505e-148, PID: 72.94 | (Putative Nitrate transporter 1:2 {ECO:0000313|EMB... [more] |
| A0A2I4FHK4 | E-Value: 7.284e-147, PID: 70.48 | (protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|Re... [more] |
| A0A6P6V692 | E-Value: 1.157e-146, PID: 69.84 | (protein NRT1/ PTR FAMILY 4.6-like {ECO:0000313|Re... [more] |
| A0A6P6UXC9 | E-Value: 1.603e-146, PID: 66.87 | (protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO... [more] |
| A0A6P5XDH3 | E-Value: 2.084e-146, PID: 70.23 | (protein NRT1/ PTR FAMILY 4.6-like isoform X1 {ECO... [more] |
| A0A833U3U8 | E-Value: 1.043e-145, PID: 70.48 | (Uncharacterized protein {ECO:0000313|EMBL:KAF5449... [more] |
| A0A068TVL7 | E-Value: 2.879e-145, PID: 66.87 | (Uncharacterized protein {ECO:0000313|EMBL:CDP0028... [more] |
| A0A5J5AHX0 | E-Value: 1.051e-144, PID: 70.72 | (Uncharacterized protein {ECO:0000313|EMBL:KAA8529... [more] |
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back to topAnalysis:
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |