MASEDRAEASDPSLSRNLSSSRLNAKAAEFVPRSAEFVYHKAPGSGYFPA
QAHVRGNQRGFTQQYVPVVQYHQPQQAPQPLVQAVSAKKSAGDGTKTEIT
DEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIK
AFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAE
NLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY
YSSKVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEAD
TSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGL
IAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRP
QHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGR
GKPVVV*
| Relationships |
|---|
| The polypeptide, DCAR_027495, derives from mRNA, DCAR_027495. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A6P5YVP0 ((la-related protein 6B-like isoform X1 {ECO:0000313|RefSeq:XP_022744623.1}))
HSP 1 Score: 401.749 bits (1031), Expect = 3.679e-134
Identity = 239/503 (47.51%), Postives = 293/503 (58.25%), Query Frame = 0
Query: 11 DPSLSRNLSSSRLNAKAAEFVP-RSAEFVYHKAPGSGYFPAQAHVRGNQRGFTQQYVPVVQYHQPQQAPQPL--------------------------------VQAV-------SAKKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGGS--SSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
DP LS NLSSSRLNA+A EFVP RS + G + + P +H P +P P+ ++AV K +K +++EAT K++NQVEYYFSD+NLATTDHLMRFINKDP+GYVPLSVV SFKKIKA +++ +QLA +L+NS KLVVSEDGKKVRR PLT++DMEELQSRIV+AENLPEDHCHQNLMKIFSAVGSVK+IRTCQPQPS G SS S+ AK+DG+++ Y S V ELN+EG WR GL+VRL+ + A+ AH RGKK G GE +E D ST E+ NEK EDPS++ D S + YP GEEH+++KE G+ KGR RGR KGRGR +HN++ N RGNHV +S + V+ E +V K PGPRMPDGTRGF+MGRGKPV V
Sbjct: 20 DPHLSWNLSSSRLNAQAPEFVPTRSTPL--QQQQNHRMMIPPPPPPGMLQVYPPLPPPPTAFHLPVHSPVPMPRHMVPVHHHVPMRNHHHPYQNHHHHHHREQEIEAVVRKDQQKEHKDHGATSSKNVLSEEATLKILNQVEYYFSDLNLATTDHLMRFINKDPEGYVPLSVVASFKKIKALITTHSQLASVLQNSLKLVVSEDGKKVRRQHPLTEADMEELQSRIVVAENLPEDHCHQNLMKIFSAVGSVKTIRTCQPQPSGSGVSSASRSAKSDGLSFSNKLHAYVEYESVELAEKAVAELNDEGNWRIGLRVRLMLRRASKPAHARGKK-GHDGEGHYEEDDASTSEQQSNEKQQEDPSRLSDIHSHE-------------YP--------------------------------GEEHSNDKECGQKKGRNRGRCKGRGRVHYHNNHHNSRGNHVSTPPLASSNLVSDEQASVAKQAPGPRMPDGTRGFAMGRGKPVSV 474
Match: A0A5N6QYY8 (HTH La-type RNA-binding domain-containing protein {ECO:0000259|PROSITE:PS50961})
HSP 1 Score: 384.8 bits (987), Expect = 3.688e-128
Identity = 217/387 (56.07%), Postives = 275/387 (71.06%), Query Frame = 0
Query: 70 QYHQPQQAPQPLVQAVSAKKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNYYSSKVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
Q+++ + APQ +++ A S+ K ++++EAT K++NQVEYYFSDVNLATTDHLMRFIN+DP+G+VP+SVV SFKKIKA +SS +QLA ILRNS+KLVVSEDGKKV+R PLT+SDMEELQSRI++AENLPEDHCHQNLMK+FSAVGSVK+IRTCQPQ SNGG+SS S+ K+DG+ +S+KV+ELN+EG WR+GL+VRL+ + + ++ RGKK G GE+ ++ DTST ++ NEK +E P Q D A+ Y + GEEH +EK+GG+ +GR RGRGKGRGR QHH+ N+RGNHVG S + N+E PTV K PPGPRMPDGTRGFSMGRGKPV V
Sbjct: 99 QFYEQEVAPQAQIESDHAPASSN---KNKLSEEATVKVLNQVEYYFSDVNLATTDHLMRFINRDPEGFVPISVVGSFKKIKALISSHSQLATILRNSSKLVVSEDGKKVKRQHPLTESDMEELQSRIIVAENLPEDHCHQNLMKVFSAVGSVKTIRTCQPQTSNGGASSASRSGKSDGM-LFSNKVSELNDEGNWRSGLRVRLMLRRTSKASTSRGKK-GHDGEVNFEDDDTSTSDQQQNEKQLEYPYQQSD---------------------------------------------AHPYEYTGEEHVNEKDGGQRRGRSRGRGKGRGRGQHHH---NNRGNHVGTPPSSNICNNELPTVAKQPPGPRMPDGTRGFSMGRGKPVAV 432
Match: A0A067K088 ((Uncharacterized protein {ECO:0000313|EMBL:KDP29552.1}))
HSP 1 Score: 385.185 bits (988), Expect = 4.796e-127
Identity = 207/374 (55.35%), Postives = 250/374 (66.84%), Query Frame = 0
Query: 99 ITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRG-RGKGRGRPQHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
++DEA QKL+NQVEYYFSD+NLATTDHLMRFINKDP+GYVP+SVV SFKKIKA +++ +QLA ILRNS+KLVVSEDGKKVRRL PLT+SD+EELQSRIV+AENLPEDHCH NLMKIFS+VGSVK+IRTC PQ S GG+SS S+ KADG+++ Y S V ELN+E +WR+GL+VRL+ K + RGKK G G +E + ST E+ PNEK E P Q +D +++ GE+HA++KEG KGR +G R KGRGR QHH+ N+RGNHVG S +PV SE PTV K PPGPRMPDGTRGF+MGRGKP+ V
Sbjct: 199 LSDEAVQKLLNQVEYYFSDLNLATTDHLMRFINKDPEGYVPISVVASFKKIKAAINNNSQLASILRNSSKLVVSEDGKKVRRLHPLTESDVEELQSRIVVAENLPEDHCHHNLMKIFSSVGSVKTIRTCPPQTSGGGASSASRSTKADGMHFSNKLHAFVEYESVEIAEKAVIELNDEESWRSGLRVRLMLKRTSKHTQARGKK-GHDGSGHSEEDEASTSEQQPNEKQHESPPQHHD---------------------------------------------VHSHEQSGEDHANDKEGAHRKGRNKGHRNKGRGRGQHHH---NNRGNHVGTPPSNNPVMSELPTVAKQPPGPRMPDGTRGFAMGRGKPLAV 523
Match: A0A6A3AHR8 ((Chloroplast outer envelope protein 37 isoform 1 {ECO:0000313|EMBL:KAE8704130.1}))
HSP 1 Score: 381.719 bits (979), Expect = 2.241e-126
Identity = 204/372 (54.84%), Postives = 250/372 (67.20%), Query Frame = 0
Query: 100 TDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
++EA K++NQVEYYFSD+NLATTDHLMRFINKDP+GY P+SV SFKKIKA V++ +QLA +LRNS+KLVVSEDGKKVRRLLPLT+SDMEELQSRI++AENLPEDHCHQNLMKIFSAVGSVK+IRTCQPQPS GG S S+ AK+DG+++ Y S V ELN+EG WR+GL+VRL+ + A H R KK G G+ Q +E + S E N+K EDPSQ++D +++ GEEH ++KEGG+ KGR RG GKGRGR Q+H ++ N GN VG +S + V++E PTV K PGPRMPDGTRGF+MGRGKPV V
Sbjct: 145 SEEAALKILNQVEYYFSDLNLATTDHLMRFINKDPEGYAPISVAASFKKIKALVTTHSQLATVLRNSSKLVVSEDGKKVRRLLPLTESDMEELQSRIIVAENLPEDHCHQNLMKIFSAVGSVKTIRTCQPQPSGGGLSPASRSAKSDGMHFSNKLHAFVEYESVELAEKAVVELNDEGNWRSGLRVRLMLQNALQPVHARAKK-GHNGKGQCEEYEVSNTEMHSNKKQ-EDPSQLFD---------------------------------------------IHSHGNPGEEHPNDKEGGQKKGRNRGWGKGRGRTQYHINHHNSHGNQVGSPASSNWVSNEQPTVAKQTPGPRMPDGTRGFAMGRGKPVAV 469
Match: A0A061EPK5 ((RNA-binding protein, putative isoform 2 {ECO:0000313|EMBL:EOY06990.1}))
HSP 1 Score: 378.637 bits (971), Expect = 7.470e-126
Identity = 216/386 (55.96%), Postives = 261/386 (67.62%), Query Frame = 0
Query: 88 KKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGG--SSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
K +K +++EAT K++NQVEYYFSD+NLATTDHLMRFINKDP+GYVP+SVV SFKKIKA +++ QLA +LRNS KLVVSEDGKKVRR PLT+SDMEELQSRI++AENLPEDHCHQNLMKIFS+VGSVK+IRTCQPQPS GG SS S+ AK+DG+++ Y S V ELN+EG WR+GL+VRL+ + + H RGKK G GE +E D ST E+ N+K EDPSQ+ D S + YP GEEH++EKEGG+ KGR RGRGKGRGR Q+HN+ N RGNHVG S++ + V++E TV K PPGPRMPDGTRGF+MGRGKPV V
Sbjct: 89 KDHGASCSKNGLSEEATLKILNQVEYYFSDLNLATTDHLMRFINKDPEGYVPISVVASFKKIKALITTHAQLATVLRNSLKLVVSEDGKKVRRQHPLTESDMEELQSRIIVAENLPEDHCHQNLMKIFSSVGSVKTIRTCQPQPSGGGVSSASRTAKSDGMHFSNKLHAFVEYESVELAEKAVAELNDEGNWRSGLRVRLMLRRVSKPTHTRGKK-GHDGEGHYEEDDASTSEQQSNDKQQEDPSQLSDIHSHE-------------YP--------------------------------GEEHSNEKEGGQKKGRNRGRGKGRGRAQYHNNQHNSRGNHVGTPPSATSNLVSNEQATVAKQPPGPRMPDGTRGFAMGRGKPVAV 428
Match: A0A061EXJ3 ((RNA-binding protein, putative isoform 1 {ECO:0000313|EMBL:EOY06989.1}))
HSP 1 Score: 379.022 bits (972), Expect = 5.415e-125
Identity = 216/386 (55.96%), Postives = 261/386 (67.62%), Query Frame = 0
Query: 88 KKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGG--SSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
K +K +++EAT K++NQVEYYFSD+NLATTDHLMRFINKDP+GYVP+SVV SFKKIKA +++ QLA +LRNS KLVVSEDGKKVRR PLT+SDMEELQSRI++AENLPEDHCHQNLMKIFS+VGSVK+IRTCQPQPS GG SS S+ AK+DG+++ Y S V ELN+EG WR+GL+VRL+ + + H RGKK G GE +E D ST E+ N+K EDPSQ+ D S + YP GEEH++EKEGG+ KGR RGRGKGRGR Q+HN+ N RGNHVG S++ + V++E TV K PPGPRMPDGTRGF+MGRGKPV V
Sbjct: 158 KDHGASCSKNGLSEEATLKILNQVEYYFSDLNLATTDHLMRFINKDPEGYVPISVVASFKKIKALITTHAQLATVLRNSLKLVVSEDGKKVRRQHPLTESDMEELQSRIIVAENLPEDHCHQNLMKIFSSVGSVKTIRTCQPQPSGGGVSSASRTAKSDGMHFSNKLHAFVEYESVELAEKAVAELNDEGNWRSGLRVRLMLRRVSKPTHTRGKK-GHDGEGHYEEDDASTSEQQSNDKQQEDPSQLSDIHSHE-------------YP--------------------------------GEEHSNEKEGGQKKGRNRGRGKGRGRAQYHNNQHNSRGNHVGTPPSATSNLVSNEQATVAKQPPGPRMPDGTRGFAMGRGKPVAV 497
HSP 2 Score: 37.3502 bits (85), Expect = 6.685e+0
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 0
Query: 14 LSRNLSSSRLNAKAAEFVP 32
LSRNLSSSRLNA+A EFVP
Sbjct: 21 LSRNLSSSRLNAQAPEFVP 39
Match: A0A5B6ZD80 ((Putative Lupus La protein {ECO:0000313|EMBL:MPA41974.1}) (Fragment))
HSP 1 Score: 377.867 bits (969), Expect = 5.646e-125
Identity = 248/509 (48.72%), Postives = 300/509 (58.94%), Query Frame = 0
Query: 10 SDPSLSRNLSSSRLNAKAAEFVPRSA------------EFVYHKAPGSG------------YFPAQAHVRGNQRGFTQQYVPV-------------------VQYHQPQQAPQPLVQAVSAKKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGG---------------SSSGSKVAKADGVNYYSSKVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKV---GQGGELQRKEADTSTRE-EDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
SDPSLSRN+S SRLNA+AAEFVPRS+ V +AP G + P Q+HV Q + P V + Q+ P Q + G++ + +EATQK++NQVEYYFSDVNLATTDHLMRFINKDP+GYVP++VV +FKKIKA +SS +QLA ILRNS KLVVSEDGKKVRR PLT+SDMEELQSRIVIAENLPEDHCHQNLMKIFSA+GSVK+IRTCQPQ SNGG S+ + + + + V ELN EG WR+GL+VRL + A SA VR KKV G+G E +E DTST + + P+EK++ED SQ D QS + GE+ A++KEGG+ KGR RGRGKGRGR Q+H+ N+RG+HVG S + VN+E P V K PPGPRMPDGTRGFSMGRGKP+VV
Sbjct: 1 SDPSLSRNVSFSRLNAQAAEFVPRSSSSSSSSSTMPQPRLVIPQAPPPGLVHVYPAPNSPFHVPIQSHVAVPSHVTVQNHHPHPHHVQVQYHHHHHRQYYGGVGFGDHQEVAAPAQQQAPVEPDHVSGSRNGLFEEATQKILNQVEYYFSDVNLATTDHLMRFINKDPEGYVPITVVAAFKKIKALISSNSQLATILRNSAKLVVSEDGKKVRRQHPLTESDMEELQSRIVIAENLPEDHCHQNLMKIFSAIGSVKTIRTCQPQASNGGASSASRSAKADSMLYSNKLHAFVEYESIEFAEKAVAELNNEGNWRSGLRVRLFLRRTAKSAQVRTKKVGHEGEGREANYEEDDTSTSDTQQPSEKNLEDFSQQSDAQSHE---------------------------------------------HTGEDLANDKEGGQRKGRSRGRGKGRGRAQYHH---NNRGSHVGTPPSNNSVNTEQPIVAKQPPGPRMPDGTRGFSMGRGKPIVV 461
Match: A0A699I468 ((La-related protein 6B {ECO:0000313|EMBL:GEZ15811.1}))
HSP 1 Score: 376.326 bits (965), Expect = 8.787e-125
Identity = 247/473 (52.22%), Postives = 295/473 (62.37%), Query Frame = 0
Query: 12 PSLSRNLSSSRLNAKAAEFVPRSAEFVY---HKAPGSGYFPAQAHVRGNQRGFTQQYVPVVQYHQPQQAPQPLVQAVSAKKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSK-VAKADGVNYYSSKV----------------TELNEEGTWRNGLKVRLLNKPAATSAHVRGK--KVGQGGELQRKEADTSTREE-DPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQH-----HNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
PSL+R+LS +LNAKA EFVPR+A P P HV Q G +V +P V + K A K DE+TQK+INQVEYYFSD+NLATTDHLMRFI+KDP+GYVP+SVV SFKKIKA VS QLA ILRNS KLVVSEDGKKV+R PLT++DMEELQSRI++AENLPEDHCHQNLMKIFSAVGSVK+IRTCQPQ SNGG++S S+ +KAD + ++S+K+ ELN+EG WRN LKVRLL +P A A RGK K GQ E KE DTST E+ PNEKH++D Q + QS + +V QGE+H++EKEGG+ KGR RGRGKGRGRPQ+ H +N N+RG H+ S + VN+EHP + K PPGPRMPDGTRGFSMGRGKP+ V
Sbjct: 26 PSLTRSLS--KLNAKAPEFVPRAAPAAVVSPPPQPPPQNTPGIIHVMIRQXGGGTGFV------------EPNVAVSAEAKEASLSVK----DESTQKIINQVEYYFSDINLATTDHLMRFISKDPEGYVPISVVVSFKKIKALVSGHAQLATILRNSGKLVVSEDGKKVKRQHPLTEADMEELQSRIIVAENLPEDHCHQNLMKIFSAVGSVKTIRTCQPQASNGGATSASRGASKADAM-HFSNKLHAFVEYETIELAEKAALELNKEGNWRNSLKVRLLRRP-AKPAQARGKKEKAGQEDEAVHKEDDTSTSEQHTPNEKHLDDTHQQSEVQS-------------------------------------------HEHV-QGEDHSNEKEGGQKKGRNRGRGKGRGRPQYSNHNNHYNNHNNRGAHLVTPPSSNSVNNEHPVIGKQPPGPRMPDGTRGFSMGRGKPISV 434
Match: A0A061EQ58 ((RNA-binding protein, putative isoform 3 {ECO:0000313|EMBL:EOY06991.1}))
HSP 1 Score: 377.867 bits (969), Expect = 1.389e-124
Identity = 216/387 (55.81%), Postives = 261/387 (67.44%), Query Frame = 0
Query: 88 KKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS-------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGG--SSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
K +K +++EAT K++NQVEYYFSD+NLATTDHLMRFINKDP+GYVP+SVV SFKKIKA +++ QLA +LRNS KLVVSEDGKKVRR PLT+SDMEELQSRI++AENLPEDHCHQNLMKIFS+VGSVK+IRTCQPQPS GG SS S+ AK+DG+++ Y S V ELN+EG WR+GL+VRL+ + + H RGKK G GE +E D ST E+ N+K EDPSQ+ D S + YP GEEH++EKEGG+ KGR RGRGKGRGR Q+HN+ N RGNHVG S++ + V++E TV K PPGPRMPDGTRGF+MGRGKPV V
Sbjct: 158 KDHGASCSKNGLSEEATLKILNQVEYYFSDLNLATTDHLMRFINKDPEGYVPISVVASFKKIKALITTHAQLATVLRNSLKLVVSEDGKKVRRQHPLTESDMEELQSRIIVAENLPEDHCHQNLMKIFSSVGSVKTIRTCQPQPSGGGVSSASRTAKSDGMHFSNKLHAFVEYESVELAEKAVVAELNDEGNWRSGLRVRLMLRRVSKPTHTRGKK-GHDGEGHYEEDDASTSEQQSNDKQQEDPSQLSDIHSHE-------------YP--------------------------------GEEHSNEKEGGQKKGRNRGRGKGRGRAQYHNNQHNSRGNHVGTPPSATSNLVSNEQATVAKQPPGPRMPDGTRGFAMGRGKPVAV 498
HSP 2 Score: 37.3502 bits (85), Expect = 6.747e+0
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 0
Query: 14 LSRNLSSSRLNAKAAEFVP 32
LSRNLSSSRLNA+A EFVP
Sbjct: 21 LSRNLSSSRLNAQAPEFVP 39
Match: A0A5N6QZU1 ((Uncharacterized protein {ECO:0000313|EMBL:KAE8022738.1}))
HSP 1 Score: 374.4 bits (960), Expect = 6.668e-124
Identity = 217/402 (53.98%), Postives = 274/402 (68.16%), Query Frame = 0
Query: 70 QYHQPQQAPQPLVQAVSAKKSAGDGTKTEITDEATQKLINQVEYYFSDVNLATTDHLMRFINKDPDGYVPLSVVFSFKKIKAFVSSTTQLAGILRNSTKLVVSEDGKKVRRLLPLTDSDMEELQSRIVIAENLPEDHCHQNLMKIFSAVGSVKSIRTCQPQPSNGGSSSGSKVAKADGVNY---------YSS------KVTELNEEGTWRNGLKVRLLNKPAATSAHVRGKKVGQGGELQRKEADTSTREEDPNEKHIEDPSQMYDGQSAQVNEQKIKALAVVYYPSGEIKQGGLIAVDGETNEEQDGVFNAYNYVWQGEEHASEKEGGRGKGRGRGRGKGRGRPQHHNHNQNHRGNHVGGSSSVHPVNSEHPTVFKPPPGPRMPDGTRGFSMGRGKPVVV 456
Q+++ + APQ +++ A S+ K ++++EAT K++NQVEYYFSDVNLATTDHLMRFIN+DP+G+VP+SVV SFKKIKA +SS +QLA ILRNS+KLVVSEDGKKV+R PLT+SDMEELQSRI++AENLPEDHCHQNLMK+FSAVGSVK+IRTCQPQ SNGG+SS S+ K+DG+ + Y S V+ELN+EG WR+GL+VRL+ + + ++ RGKK G GE+ ++ DTST ++ NEK +E P Q D A+ Y + GEEH +EK+GG+ +GR RGRGKGRGR QHH+ N+RGNHVG S + N+E PTV K PPGPRMPDGTRGFSMGRGKPV V
Sbjct: 99 QFYEQEVAPQAQIESDHAPASSN---KNKLSEEATVKVLNQVEYYFSDVNLATTDHLMRFINRDPEGFVPISVVGSFKKIKALISSHSQLATILRNSSKLVVSEDGKKVKRQHPLTESDMEELQSRIIVAENLPEDHCHQNLMKVFSAVGSVKTIRTCQPQTSNGGASSASRSGKSDGMLFSNKWHAFVEYESVELAEKAVSELNDEGNWRSGLRVRLMLRRTSKASTSRGKK-GHDGEVNFEDDDTSTSDQQQNEKQLEYPYQQSD---------------------------------------------AHPYEYTGEEHVNEKDGGQRRGRSRGRGKGRGRGQHHH---NNRGNHVGTPPSSNICNNELPTVAKQPPGPRMPDGTRGFSMGRGKPVAV 448
| Match Name | Stats | Description |
|---|---|---|
| A0A6P5YVP0 | E-Value: 3.679e-134, PID: 47.51 | (la-related protein 6B-like isoform X1 {ECO:000031... [more] |
| A0A5N6QYY8 | E-Value: 3.688e-128, PID: 56.07 | HTH La-type RNA-binding domain-containing protein ... [more] |
| A0A067K088 | E-Value: 4.796e-127, PID: 55.35 | (Uncharacterized protein {ECO:0000313|EMBL:KDP2955... [more] |
| A0A6A3AHR8 | E-Value: 2.241e-126, PID: 54.84 | (Chloroplast outer envelope protein 37 isoform 1 {... [more] |
| A0A061EPK5 | E-Value: 7.470e-126, PID: 55.96 | (RNA-binding protein, putative isoform 2 {ECO:0000... [more] |
| A0A061EXJ3 | E-Value: 5.415e-125, PID: 55.96 | (RNA-binding protein, putative isoform 1 {ECO:0000... [more] |
| A0A5B6ZD80 | E-Value: 5.646e-125, PID: 48.72 | (Putative Lupus La protein {ECO:0000313|EMBL:MPA41... [more] |
| A0A699I468 | E-Value: 8.787e-125, PID: 52.22 | (La-related protein 6B {ECO:0000313|EMBL:GEZ15811.... [more] |
| A0A061EQ58 | E-Value: 1.389e-124, PID: 55.81 | (RNA-binding protein, putative isoform 3 {ECO:0000... [more] |
| A0A5N6QZU1 | E-Value: 6.668e-124, PID: 53.98 | (Uncharacterized protein {ECO:0000313|EMBL:KAE8022... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |