DCAR_029577 | CarrotOmics

Resource Type:

Polypeptide

Name:

DCAR_029577

Identifier:

DCAR_029577.mRNA-protein

Sequence:

MDGPPAFAPSPAIKAHQAHQTGYPINVWPKPTTFEWTHPESVPLAPSFTI
TSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDI
SAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPG
RVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHW
HITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVL
PEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPL
NPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNG
TLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTIL
QTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGT
DQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADAT
VLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIG
AEAIQPLWCIRNPTMCNTVQDV*

Sequence Length:

573

Sequence Checksum:

0c871f974bd28870be91615e02944e28

View location in JBrowse:

DCARv2_Chr9:9247577..9251785-

Organism:

Daucus carota subspecies sativus

Relationship:

There is 1 relationship.
Relationships
The polypeptide, DCAR_029577, derives from mRNA, DCAR_029577.

Blast Results:

The following BLAST results are available for this feature:

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YPD5 (Beta-N-acetylhexosaminidase {ECO:0000256|ARBA:ARBA00012663} (3.2.1.52 {ECO:0000256|ARBA:ARBA00012663}) (Fragment))

HSP 1 Score: 896.345 bits (2315), Expect = 0.000e+0
Identity = 410/547 (74.95%), Postives = 472/547 (86.29%), Query Frame = 0

Query:   24 PINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            PINVWPKP TF W HP+++ L+P FTITSP H+YLT AVNRY+RQ+L EHH PLITP+V+     PL+SL ++V+D+S PL HGV+ESY LT+P  G  +A +TA T W AMRGLETFSQLVWG+P RV +GLFISD+P+F HRGV+LDTSRN++ V+D+ RLI  +S NK+NVFHWHITDSHSFP+V+PSEP L+ KGSYG  M+Y+PEDVKR+VEFG EHGVRVLPEIDMPGHTGSWAEAYPEIV CA MFWWPAG+EW+DRLASEPG G LNPL PKTYEVLKNVI D   MFPEPF+HAGADE+IPGCWK D T+Q FL+NNGTLSQ+LE+F+N+T+PYIVS NRTVVYWEDV+LDD +KV+P  LP E TILQTWNNGPNNTKRIV +GY  IVSS+DFYYLDCGHG F+GN S YAQ+PG+DQG GGSWC PFKTWQTIYNYDITYGLS+E+AKLVLGGEVALW+EQAD TVLD R+WPRASA+AE LWSGN DE+GMKRYAEATDRL EWR RMV RG+GAE IQPLWC+RNP MCNTV 
Sbjct:   53 PINVWPKPITFSWPHPQAISLSPIFTITSPNHQYLTPAVNRYLRQVLTEHHRPLITPAVNLTLASPLQSLTLTVSDLSTPLQHGVNESYTLTIPAVGS-SANLTAETVWGAMRGLETFSQLVWGDPSRVAAGLFISDSPIFEHRGVMLDTSRNYYEVEDLQRLIGAMSANKLNVFHWHITDSHSFPLVLPSEPNLAAKGSYGTEMLYSPEDVKRIVEFGLEHGVRVLPEIDMPGHTGSWAEAYPEIVTCANMFWWPAGTEWSDRLASEPGTGHLNPLTPKTYEVLKNVIHDATIMFPEPFYHAGADEIIPGCWKADPTVQAFLSNNGTLSQLLEMFVNSTFPYIVSLNRTVVYWEDVLLDDNVKVNPAVLPPENTILQTWNNGPNNTKRIVASGYRAIVSSADFYYLDCGHGDFLGNSSLYAQEPGSDQGNGGSWCGPFKTWQTIYNYDITYGLSEEEAKLVLGGEVALWSEQADPTVLDPRIWPRASALAETLWSGNRDETGMKRYAEATDRLNEWRYRMVARGVGAEPIQPLWCVRNPGMCNTVN 598

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BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YPQ6 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 895.19 bits (2312), Expect = 0.000e+0
Identity = 411/547 (75.14%), Postives = 473/547 (86.47%), Query Frame = 0

Query:   24 PINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            PINVWPKP TF W HP+++ L+P FTITSP H+YLT AVNRY+RQ+L EHH PLITP+V+     PL+SL ++V+D+S PL HGV+ESY LT+P  G  +A +TA T W AMRGLETFSQLVWG+P RV + LFISD+P+F HRGV+LDTSRN++ V+D++RLI  +S NK+NVFHWHITDSHSFP+V+PSEP L+ KGSYG  M Y+PEDVKR+VEFG EHGVRVLPEIDMPGHTGSWAEAYPEIV CA MFWWPAG+EW+DRLASEPG G LNPLNPKTYE+LKNVI DV  MFPEPF+HAG DE+IPGCWK D +IQTFL+NNGTLSQ+LE+F+N+T+PYIVS NRTVVYWEDV+LD  +KV+PT LP E TILQTWNNGPNNTKRIV +GY  IVSS+DFYYLDCGHGGF+GNDSQY Q+PG+DQ  GGSWC PFKTWQTIYNYDITYGLS+E+AKLVLGGEVALW+EQAD  VLDSR+WPRASAMAEALWSGN DE+GMKRYAEAT+RL EW  RMV RGIGAE IQPLWC+RNP MCN V 
Sbjct:   32 PINVWPKPITFSWPHPQAISLSPIFTITSPNHQYLTPAVNRYLRQVLTEHHRPLITPAVNLTLASPLQSLTLTVSDLSTPLQHGVNESYTLTIPAVGS-SANLTAETVWGAMRGLETFSQLVWGDPSRVAAWLFISDSPIFEHRGVMLDTSRNYYQVEDLLRLIGAMSANKLNVFHWHITDSHSFPLVLPSEPNLAAKGSYGTEMQYSPEDVKRIVEFGLEHGVRVLPEIDMPGHTGSWAEAYPEIVTCANMFWWPAGTEWSDRLASEPGTGHLNPLNPKTYEILKNVIHDVATMFPEPFYHAGTDEIIPGCWKADPSIQTFLSNNGTLSQLLEMFVNSTFPYIVSLNRTVVYWEDVLLDAIVKVNPTILPPEKTILQTWNNGPNNTKRIVASGYRAIVSSADFYYLDCGHGGFLGNDSQYDQQPGSDQDNGGSWCGPFKTWQTIYNYDITYGLSEEEAKLVLGGEVALWSEQADPAVLDSRIWPRASAMAEALWSGNRDETGMKRYAEATNRLNEWSYRMVARGIGAEPIQPLWCVRNPGMCNIVH 577

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BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J4ZDC6 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 886.715 bits (2290), Expect = 0.000e+0
Identity = 408/547 (74.59%), Postives = 472/547 (86.29%), Query Frame = 0

Query:   24 PINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            PINVWPKP  F W HP ++PL+P FTITSP ++YLT AV+RY+RQ L EHH PLITP+V+  ST PL+SL ++V+D+S P  HGV+ESY LT+P A G +A +TA T W AMRGLETFSQLVWG+P  V +GLFISD+P+F HRGV+LDTSRN++ VDD++RLI  +S NK+NVFHWHITDSHSFP+V+PSEP L+ KGSYG  M Y+P+DVKR+V+FG EHGVRVLPEIDMPGHTGSWAEAYPEIVACA MFWWPAG++W DR ASEPG G LNPLN KTYE+LKNVI D   MFPE F+HAGADE+IPGCWK D +IQTFL+NNGTLSQ+LE+F+N+T+PYIVS NRTVVYWEDV+LD  +KV+P  LP E TILQTWNNGPNNTKRIV +GY  IVSSSDFYYLDCGHG F+GN+SQYAQ+PG+DQG GGSWC PFKTWQTIYNYDITYGLS+E+AKLVLGGEVALW+EQAD +VLD R+WPRASAMAEALWSGN DE+GM RYAEATDRL EWR RMV RG+GAE IQPLWC+RNP MCNTVQ
Sbjct:   33 PINVWPKPRIFSWPHPLAIPLSPVFTITSPNNQYLTPAVDRYLRQALTEHHRPLITPAVNLTSTSPLQSLALTVSDLSTPHQHGVNESYALTIP-ADGSSANLTAETVWGAMRGLETFSQLVWGDPSLVAAGLFISDSPIFEHRGVMLDTSRNYYQVDDLLRLIGAMSANKLNVFHWHITDSHSFPLVLPSEPNLAAKGSYGAEMQYSPDDVKRIVDFGLEHGVRVLPEIDMPGHTGSWAEAYPEIVACANMFWWPAGTQWPDRFASEPGTGHLNPLNLKTYEILKNVIHDTSIMFPETFYHAGADEIIPGCWKADPSIQTFLSNNGTLSQLLEMFVNSTFPYIVSLNRTVVYWEDVLLDANVKVNPAVLPPEKTILQTWNNGPNNTKRIVASGYRAIVSSSDFYYLDCGHGAFIGNNSQYAQQPGSDQGNGGSWCGPFKTWQTIYNYDITYGLSEEEAKLVLGGEVALWSEQADPSVLDPRIWPRASAMAEALWSGNRDETGMLRYAEATDRLNEWRYRMVTRGVGAEPIQPLWCVRNPGMCNTVQ 578

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YPC4 (Beta-N-acetylhexosaminidase {ECO:0000256|ARBA:ARBA00012663} (3.2.1.52 {ECO:0000256|ARBA:ARBA00012663}) (Fragment))

HSP 1 Score: 870.152 bits (2247), Expect = 0.000e+0
Identity = 401/535 (74.95%), Postives = 463/535 (86.54%), Query Frame = 0

Query:   36 WTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            W HP+++PL+P FTITSP H+YL AAVNRY+RQ+L EHH  LITP+V+  S  PL++L I+V D++APL HGV+ESY LT+P AG  +A +TA T W AMRGLETFSQLVWG+P RV +GLFISD P+F HRGV+LDTSRN++ V+D++RL+  +S NK+NVFHWHITDSHSFP+V+PSEP L+ KGSYG  M Y+PEDVKR+VEFG EHGVRVLPEIDMPGHTGSWAEAYPEIV CA MFWWPAG+EW+DRLASEPG G LNPL PKTYEVLKNVI D   MFPEPF+HAGADE+IPGCWK D T+Q FL+NNGTLSQ+LE+F+N+T+PYIVS NRTVVYWEDV+LDD +KV+P  LP E TILQTWNNGPNNTKRIV +GY  IVSS+DFYYLDCGHG F+GN S YAQ+PG+DQG GGSWC PFKTWQT+YNYDITYGLS+E+AKLVLGGEVALW+EQAD  VLDSR+WPRASAMAEALWSGN DE+GMKRYAEAT+RL EW  RMV RGIGAE IQPLWC+RNP MCNTV 
Sbjct:    1 WPHPQAIPLSPFFTITSPNHQYLIAAVNRYLRQVLTEHHRHLITPAVNHESPSPLQTLTITVNDLTAPLQHGVNESYTLTIPTAGS-SANLTAETVWGAMRGLETFSQLVWGDPTRVTAGLFISDWPIFEHRGVMLDTSRNYYEVEDLLRLVGAMSANKLNVFHWHITDSHSFPLVLPSEPDLAAKGSYGPEMQYSPEDVKRIVEFGLEHGVRVLPEIDMPGHTGSWAEAYPEIVTCANMFWWPAGTEWSDRLASEPGTGHLNPLTPKTYEVLKNVIHDATIMFPEPFYHAGADEIIPGCWKADPTVQAFLSNNGTLSQLLEMFVNSTFPYIVSLNRTVVYWEDVLLDDNVKVNPAVLPPENTILQTWNNGPNNTKRIVASGYRAIVSSADFYYLDCGHGDFLGNSSLYAQEPGSDQGNGGSWCGPFKTWQTMYNYDITYGLSEEEAKLVLGGEVALWSEQADPAVLDSRIWPRASAMAEALWSGNRDETGMKRYAEATNRLNEWSYRMVARGIGAEPIQPLWCVRNPGMCNTVN 534

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J7HJC7 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 864.759 bits (2233), Expect = 0.000e+0
Identity = 395/550 (71.82%), Postives = 467/550 (84.91%), Query Frame = 0

Query:   21 TGYPINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            T  PIN+WPKP TF W  P+S+ L+P+FTI+SP H+YLT AVNRY+RQ+L EHH PL+ P+V++ +  PL++L ++V D++ PL HGV+ESY+LT+P +G  T  +TA T W AMRGLE+FSQL WG+P +V +G++ISD P+F HRGV+LDTSRN++ V+DI R I  +S NK+NVFHWHITDS SFP+V+P EP L+ KGSYG GM Y+PEDVKRVVEFG EHGVRV+PEIDMP HTGSWAEAYPEIV CA MFWWPAG  WADRLASEPG G LNPLNPKTYEV+KNVI+D   MFPEPF+HAGADE++PGCWK D +IQTFLANNGTLSQ+LE+F+N+T+PYIVS NRTVVYWEDV+LD   KV+P+ LP E TILQTWN+GPNNTK+IV++GY  IVSSSDFYYLDCGHG F+GNDS Y QKPG+DQG GGSWC PFKTWQTIYNYDITYGLS+E+ KLV+GGEVALW+EQAD TVLD R+WPRASAMAE LWSGN DE+GMKR  EATDRL EWR RMV+RG+GAE IQPLWC+RNP MCNTV 
Sbjct:   26 TQSPINIWPKPRTFSWPQPQSILLSPNFTISSPNHQYLTPAVNRYLRQVLTEHHHPLVPPTVNATAAPPLQTLTVTVADLAPPLHHGVNESYSLTIPASGAAT--LTAETVWGAMRGLESFSQLAWGDPTQVAAGVYISDFPIFEHRGVMLDTSRNYYGVEDIERTIGAMSANKLNVFHWHITDSQSFPLVLPLEPNLASKGSYGTGMEYSPEDVKRVVEFGLEHGVRVMPEIDMPAHTGSWAEAYPEIVTCANMFWWPAGFSWADRLASEPGTGHLNPLNPKTYEVIKNVIQDAATMFPEPFYHAGADEIVPGCWKADPSIQTFLANNGTLSQLLEMFVNSTFPYIVSLNRTVVYWEDVILDANAKVNPSILPPENTILQTWNDGPNNTKKIVSSGYRAIVSSSDFYYLDCGHGDFIGNDSLYDQKPGSDQGNGGSWCGPFKTWQTIYNYDITYGLSEEETKLVIGGEVALWSEQADPTVLDPRIWPRASAMAETLWSGNHDETGMKRCGEATDRLNEWRYRMVDRGVGAEPIQPLWCVRNPGMCNTVN 573

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6QKX0 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 864.759 bits (2233), Expect = 0.000e+0
Identity = 406/547 (74.22%), Postives = 467/547 (85.37%), Query Frame = 0

Query:   24 PINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            P+NVWPKP TF W  P+++ L+P+FTI+SP H+YL AAV RY+  +L EHH PL+ P+ +  S+ PL++L ++V D+ APL HGV+ESY L++P AG   A + AATPW AMRGLETFSQLVWG+P RV SG+FI D P+F HRGV+LDTSRNF++VDDI+R I+ +S NK+NVFHWHITDSHSFP+VV SEP LS KGSYG GM Y+PE+VK VVEFG EHGVRVLPEIDMPGHTGSWA AYPEIV CA MFWWPAGSEW DRLASEPG G LNPLNPKTY+V+KNVI DV  MFPEPF+HAGADE+IPGCWK D +IQTFLANNGTLSQ+LE+F+N+T+PY+VS NRTVVYWEDV+LD  IKV+P+ LP E TILQTWNNGPNNTK+IV++GY  IVSSSD+YYLDCGHG F+GNDS Y   PGTDQG GGSWC PFKTWQTIYNYDITYGL+ E+AKLVLGGEVALW+EQAD+TVLD R+WPRASAMAE LWSGN DE+GMKRYAEATDRL EWR RMV RGIGAE IQPLWCIRNP MCNTV 
Sbjct:   28 PLNVWPKPRTFSWPLPQAILLSPNFTISSPPHQYLDAAVTRYLHGVLNEHHRPLVPPAANLTSSSPLQTLTLAVADLIAPLHHGVNESYTLSIPAAG-AYASIAAATPWGAMRGLETFSQLVWGDPARVASGIFIEDFPIFEHRGVMLDTSRNFYAVDDILRTIRAMSENKLNVFHWHITDSHSFPLVVESEPDLSGKGSYGAGMEYSPENVKTVVEFGLEHGVRVLPEIDMPGHTGSWAGAYPEIVTCANMFWWPAGSEWGDRLASEPGTGHLNPLNPKTYQVIKNVINDVATMFPEPFYHAGADEIIPGCWKADPSIQTFLANNGTLSQLLEVFVNSTFPYLVSLNRTVVYWEDVLLDAEIKVNPSVLPPENTILQTWNNGPNNTKKIVSSGYRAIVSSSDYYYLDCGHGDFIGNDSTYDLPPGTDQGNGGSWCGPFKTWQTIYNYDITYGLTDEEAKLVLGGEVALWSEQADSTVLDPRIWPRASAMAETLWSGNRDETGMKRYAEATDRLNEWRHRMVARGIGAEPIQPLWCIRNPGMCNTVH 573

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6RAE9 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 864.374 bits (2232), Expect = 0.000e+0
Identity = 396/546 (72.53%), Postives = 465/546 (85.16%), Query Frame = 0

Query:   25 INVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            +NVWPKP TF W  P+++ L+ +FTI+SP HK+L AAV RY+RQ+L EHH PL+ P  +  ++ P+++L ++VTD+++PL HGV+ESY L++P A G +A + AATPW AMR LETFSQLVWG+P RV SG+FI D P+F HRGV+LDTSRNF++VDDI+R I+ +S NK+NVFHWHITDSHSFP+V+ SEP L+ KGSYG GM Y+PEDVK VVEFG +HGVRVLPEIDMPGHTGSWAEAYPEIV CA MFWWPAGS+  DRLASEPG G LNPLNPKTY+V+KNVI DV  MFPEPF+HAGADE+IPGCWK D ++QTFL NNGTLSQ+LE+F+N+T+PY+VS NRTVVYWEDV+LD  IKV P+ LP E TILQTWNNGPNNTK+IV++GY  IVSSSDFYYLDCGHG F+GN+S Y   PGTD G GGSWC PFKTWQTIYNYDITYGL+ E+AKLVLGGEVALW+EQAD+TVLD R+WPRASAMAE LWSGN DE+GMKRYAEATDRL EWR RMV RG+GAE IQPLWCIRNP MCNTV 
Sbjct:   31 LNVWPKPRTFSWPQPQAILLSKNFTISSPTHKHLDAAVTRYLRQVLTEHHRPLVPPDANLTNSPPIQTLTLAVTDLTSPLHHGVNESYTLSIP-AEGASATIAAATPWGAMRALETFSQLVWGDPARVVSGIFIEDFPIFEHRGVMLDTSRNFYAVDDILRTIRAMSENKLNVFHWHITDSHSFPLVLESEPDLAGKGSYGAGMEYSPEDVKTVVEFGLDHGVRVLPEIDMPGHTGSWAEAYPEIVTCANMFWWPAGSKLGDRLASEPGTGHLNPLNPKTYQVIKNVIDDVATMFPEPFYHAGADEIIPGCWKADPSVQTFLENNGTLSQLLEVFVNSTFPYLVSLNRTVVYWEDVLLDAEIKVKPSLLPPENTILQTWNNGPNNTKKIVSSGYRAIVSSSDFYYLDCGHGNFIGNNSMYDLLPGTDPGNGGSWCGPFKTWQTIYNYDITYGLTDEEAKLVLGGEVALWSEQADSTVLDPRIWPRASAMAETLWSGNRDETGMKRYAEATDRLNEWRHRMVARGVGAEPIQPLWCIRNPGMCNTVH 575

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A022RTI0 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 859.751 bits (2220), Expect = 0.000e+0
Identity = 394/560 (70.36%), Postives = 469/560 (83.75%), Query Frame = 0

Query:   12 AIKAHQAHQTGYPINVWPKPTTFEWTHPESVPLAPSFTITSP-YHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            +I   Q     +PINVWPKPTTF W HP+++PL+P F I++P +H++L+AAV+RY+RQIL EHH PL++P ++  S+ PLK+L+I+V D +A L HGV+ESY L +P  G   AV+TA TPW AMRGLETFSQLV+  P  V  GL ISDAPLF HRGVLLDTSRN+F +DD++RLI+ + MNK+NVFHWHITDSHSFP+V PSEP L+ KG+YG  M YT  DVK+VV++G EHGVRV+PEIDMP HTGSWAEAYPEIV CA  FWWP  SEW+DRLA+EPG GQLNPLNPKTY+V+KNVI+DV+++FP+ FFHAGADE+ P CWK D +IQ FL+  GTLSQVLEIF+N+T PYI+S NRTVVYWEDV+LD+ ++VDPTFLP+E  ILQTWNNGPNNTK+IV+ GY  IVSSSD+YYLDCGHGGF+GNDSQY Q PG +QGKGGSWCAPFKTWQTIYNYDIT GLS  +A+LV+GGEVALW+EQAD+TV+DSRVWPR SAMAEALWSGN DE G KR+AEATDRL EWR RMV RGIGAE IQPLWC++NP MCNTV 
Sbjct:   20 SISILQISAVRFPINVWPKPTTFHWPHPQAIPLSPKFIISAPRHHRHLSAAVHRYLRQILTEHHRPLVSPPLNLTSSPPLKTLKITVADTAAALAHGVNESYTLEIPSTGA-AAVLTAETPWGAMRGLETFSQLVYSKPSVVACGLRISDAPLFAHRGVLLDTSRNYFELDDLLRLIRAIGMNKLNVFHWHITDSHSFPLVTPSEPELAAKGAYGEAMKYTAADVKKVVQYGMEHGVRVVPEIDMPAHTGSWAEAYPEIVTCANEFWWPIESEWSDRLAAEPGTGQLNPLNPKTYQVVKNVIKDVVSVFPDKFFHAGADEITPNCWKTDPSIQEFLSKKGTLSQVLEIFVNSTLPYILSLNRTVVYWEDVLLDENVRVDPTFLPRENVILQTWNNGPNNTKKIVSLGYRAIVSSSDYYYLDCGHGGFLGNDSQYNQLPGFEQGKGGSWCAPFKTWQTIYNYDITSGLSGVEAELVVGGEVALWSEQADSTVMDSRVWPRGSAMAEALWSGNRDEFGKKRFAEATDRLSEWRYRMVCRGIGAEPIQPLWCVKNPGMCNTVH 578

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: F6GZ26 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 855.129 bits (2208), Expect = 0.000e+0
Identity = 400/546 (73.26%), Postives = 464/546 (84.98%), Query Frame = 0

Query:   25 INVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            INVWPKP TF W  P++  L+P+F+ITSP H++L++AV RY+R IL EHH PL+TP+V+     PL++L I V+D++APL HGV ESY L VP  G   A +TAAT W AMRGLETFSQ+VWG+P RV +GLF+ D+PLFGHRGV+LDTSRN++ V+DI+R I  +S NK+NVFHWHITDSHSFP+++PSEP L+ KGSYG  M Y+PEDVK++VEFG EHGVRVLPEID PGHTGSWAEAYPEIV CA MFWWPA +EWADRLASEPG G LNPLNPKTY+V KNVI DV A+FPEPF+H+GADE+IPGCWK D TIQTFL+N GTLSQ+LEIFIN+T+PYIVS NRTVVYWEDV+LD  +KVDP+ LP E TILQTWNNGPNNTK++V +GY  IVSSSDFYYLDCGHG F+GNDSQY QK G++   GGSWC PFKTWQTIYNYDITYGLS E+AKLVLGGEVALW+EQAD TVLD+R+WPRASAMAEALWSGN D++GMKRYA+A DRL EWR RMV RGIGAE IQPLWCIRNP MCNTV 
Sbjct:   31 INVWPKPRTFSWPSPQASLLSPNFSITSPNHQHLSSAVARYLRLILTEHHHPLVTPTVNITGP-PLETLTIIVSDLAAPLHHGVDESYTLIVPRGGA--ANLTAATVWGAMRGLETFSQIVWGDPLRVATGLFVWDSPLFGHRGVMLDTSRNYYGVEDILRTIGAMSANKLNVFHWHITDSHSFPLLLPSEPDLAGKGSYGPQMQYSPEDVKKIVEFGLEHGVRVLPEIDSPGHTGSWAEAYPEIVTCANMFWWPAEAEWADRLASEPGTGHLNPLNPKTYQVFKNVIHDVAALFPEPFYHSGADEIIPGCWKADPTIQTFLSNGGTLSQLLEIFINSTFPYIVSLNRTVVYWEDVLLDANVKVDPSMLPPENTILQTWNNGPNNTKKVVASGYRAIVSSSDFYYLDCGHGDFLGNDSQYDQKAGSNTENGGSWCGPFKTWQTIYNYDITYGLSDEEAKLVLGGEVALWSEQADPTVLDARIWPRASAMAEALWSGNQDKTGMKRYADAMDRLNEWRYRMVARGIGAEPIQPLWCIRNPGMCNTVH 573

[Close]

BLAST of DCAR_029577 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6I9TMJ1 (Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093} (3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093}))

HSP 1 Score: 853.588 bits (2204), Expect = 0.000e+0
Identity = 387/554 (69.86%), Postives = 463/554 (83.57%), Query Frame = 0

Query:   17 QAHQTGYPINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQ 570
            Q     YPI+VWPKPTTF W  P+++ L+P FTI++P H YLT AV+RY+RQIL EHH PL+TP ++     PL +L I ++D +APLTHGV ESY+LTVP  G  +AV+ A TPW AMRGLETFSQLV+  P RV  GL+ISD PLF HRG++LDT+RN++ V+D++RLI+ +SMNK+NVFHWHITDSHSFP+VVPSEP ++ KG+YG  M YT  DVKRVVE+G EHGVRV+PEIDMP HTGSWAEAYPEIV CA  FWWPA +EW DRLA+EPG GQLNPLNPKTYEV+KNV+RD ++MFP+ F+HAGADE+ P CWK+D +IQTFL+NNGTLSQ+LE+FIN+T PYI+S NRTV+YWEDV+L+  + V  + LP+E  ILQ+WNNGPNNTKRIV++GY  IVSSSDFYYLDCGHG F GN+S Y   PG+DQG GGSWC PFKTWQTIYNYDITYGL++ +AKLV+GGEVALW+EQAD+TV+DSR+WPRASAMAEALWSGN DE+G KR+AEATDRL EWR RMV RGIGAE IQPLWCI+NP MCNTV 
Sbjct:   30 QISAVRYPIDVWPKPTTFHWPQPQAITLSPEFTISAPPHSYLTPAVHRYLRQILTEHHLPLVTPLLNLTGGPPLTTLTIIISDAAAPLTHGVDESYSLTVPSTGA-SAVLNAETPWGAMRGLETFSQLVYAKPARVACGLYISDGPLFPHRGIMLDTARNYYGVEDLLRLIRTISMNKLNVFHWHITDSHSFPLVVPSEPEMAAKGAYGEDMKYTAADVKRVVEYGMEHGVRVVPEIDMPAHTGSWAEAYPEIVTCANKFWWPADAEWTDRLAAEPGTGQLNPLNPKTYEVVKNVVRDAVSMFPDQFYHAGADEITPNCWKIDPSIQTFLSNNGTLSQILEMFINSTLPYIISLNRTVIYWEDVLLNADVNVPSSLLPRENVILQSWNNGPNNTKRIVDSGYRAIVSSSDFYYLDCGHGDFTGNNSLYNMPPGSDQGNGGSWCGPFKTWQTIYNYDITYGLTEAEAKLVIGGEVALWSEQADSTVMDSRIWPRASAMAEALWSGNRDETGKKRFAEATDRLNEWRYRMVTRGIGAEPIQPLWCIKNPGMCNTVH 582


Match Name	Stats	Description
A0A5B6YPD5	E-Value: 0.000e+0, PID: 74.95	Beta-N-acetylhexosaminidase {ECO:0000256\|ARBA:ARBA... [more]
A0A5B6YPQ6	E-Value: 0.000e+0, PID: 75.14	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A5J4ZDC6	E-Value: 0.000e+0, PID: 74.59	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A5B6YPC4	E-Value: 0.000e+0, PID: 74.95	Beta-N-acetylhexosaminidase {ECO:0000256\|ARBA:ARBA... [more]
A0A7J7HJC7	E-Value: 0.000e+0, PID: 71.82	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A2R6QKX0	E-Value: 0.000e+0, PID: 74.22	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A2R6RAE9	E-Value: 0.000e+0, PID: 72.53	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A022RTI0	E-Value: 0.000e+0, PID: 70.36	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
F6GZ26	E-Value: 0.000e+0, PID: 73.26	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]
A0A6I9TMJ1	E-Value: 0.000e+0, PID: 69.86	Beta-hexosaminidase {ECO:0000256\|PIRNR:PIRNR001093... [more]

Pages

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
DCAR peptide blastp to SwissProt and TrEMBL without DCAR	This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.