MAVPLSDVGWFFGSHSKEKNVCQSQNSVAFGGGSFAVSSRVRRCNMSTMV
KSSQSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIR
YGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYS
AYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWR
GTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKF
SEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAG
IDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPY
MPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRC
CHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWW
GTQNIEPNRNNMENVTGET*
| Relationships |
|---|
| The polypeptide, DCAR_024205, derives from mRNA, DCAR_024205. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A1U8B4B0 ((phospholipase A1-Igamma1, chloroplastic-like isoform X1 {ECO:0000313|RefSeq:XP_010274199.1}))
HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 259/394 (65.74%), Postives = 321/394 (81.47%), Query Frame = 0
Query: 68 KSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRNN 461
+ +A+ W+EIHG+ DW MLDPI+PL++SELIRYG+MAQACYDAFD DP S+YCG C+F FF+ LG++ FGYDV+ YLY+ NIN P F+ KS WP WS +ANW GYVAVSNDETSASLGRRDITIAWRGTV + EW+AD+MDFLRPV+ +I DPS++VE+GFL +YTDKDE C+F +YSAREQI++E+ RL Q+Y +EE+SIT+TGHSLGSALA+LSAYDIAE G+++T DGR +P+ VFSFSGPRVGN RFKER+E LGVKVLRVVN+HD VP +PGI FNEHV ++ ++ + LPWCYSHVG EL LDH++SPFL T DP C HNLEALLHL+DGYHGKG++F S+GR+ ALVNK++DFLKD+ +PP W +N RN
Sbjct: 112 RRIADFWREIHGQGDWTGMLDPIDPLLRSELIRYGEMAQACYDAFDYDPSSRYCGSCRFMRRKFFDCLGMANFGYDVSRYLYATTNINLPNFFKKSRWPKVWSRNANWIGYVAVSNDETSASLGRRDITIAWRGTVTRLEWIADLMDFLRPVSSDKIPCPDPSVKVESGFLDVYTDKDETCRFCKYSAREQILSEVKRLTQQYPDEELSITMTGHSLGSALAMLSAYDIAETGVNVTGDGRAVPVTVFSFSGPRVGNVRFKERVERLGVKVLRVVNVHDTVPKVPGILFNEHVPELVHRLAQWLPWCYSHVGVELTLDHKHSPFLNGTGDPSCFHNLEALLHLLDGYHGKGQRFVLSSGRDPALVNKASDFLKDHYLVPPYWRQDENKGMVRNQ 505
Match: A0A835D6A5 ((Uncharacterized protein {ECO:0000313|EMBL:KAF8392283.1}))
HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 259/394 (65.74%), Postives = 322/394 (81.73%), Query Frame = 0
Query: 68 KSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRNN 461
+++++TW+EI G++DW +LDPI+PL++SELIRYG+MAQACYDAFD DP SKYCG C+F FFE G++ +GYDV+ YLY+ NIN P F+ KS W WS +ANW GYVAVSNDETS LGRRDITIAWRGTV + EW+AD+MDFLRP++ +I DP+++VE+GFL +YTDKDE C+F +YSAREQI+ E+ R+IQ+Y NEEMSITITGHSLGSALA+LSAYDI E G+++ +DGRD+P+CVFSFSGPRVGN RFK+RLE LGVKVLRVVN+HD VP +PGI FNEHV P+++K E LPWCYSHVG ELALDH+NSPFLK+T DP C HNLEA LHL+DGYHGKG +F ++GR+ ALVNK++DFLKD+ +PP W +N RN
Sbjct: 99 RAVSDTWREILGQDDWVGLLDPIDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFMRRKFFECQGMASYGYDVSRYLYATSNINLPNFFKKSRWSKVWSRNANWIGYVAVSNDETSTRLGRRDITIAWRGTVTRLEWIADLMDFLRPISSDKIPCPDPTVKVESGFLDVYTDKDENCRFCKYSAREQILTEVKRIIQQYPNEEMSITITGHSLGSALAMLSAYDIVETGLNVMEDGRDVPVCVFSFSGPRVGNVRFKDRLEALGVKVLRVVNVHDSVPKVPGILFNEHVPPVVQKFAEWLPWCYSHVGVELALDHKNSPFLKETGDPTCFHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKASDFLKDHYLVPPYWRQDENKGMIRNQ 492
Match: A0A4S4E7Z3 (Lipase_3 domain-containing protein {ECO:0000259|Pfam:PF01764})
HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 278/491 (56.62%), Postives = 353/491 (71.89%), Query Frame = 0
Query: 1 MAVPLSDVGWFF-----GSHSKEKNVCQSQNSVAFGGGSFAVSSRVRRCNMSTMVKSSQSENNMTVVIGEKAKS-------------------LAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRNNMENVTG 467
MA+PLS +G G+ +K + + + S +S R M ++ S N+ T+ + + K LA++W+EIHG++DW+ MLDPI+PL++SELIRYG+MAQACYDAFD DP SKYCG C+ +P FF+ LG ++GY+VT Y Y++YN+N P + K+L PN W SANW GYVAVSNDETSA LGRRDITIAWRGT K EW+AD D+L+ V+ +I S D ++ EAGF+Q+YTDKD CKF +YSAREQI+AE+ RLIQKY +EE+SITITGHSLGSALA ++AYDIAE G+D+ +GR P+CV+SFSGPRVGN RFKERLE LGVKVLRV N+HDKVP +PGIFFNE V P+M++VGE+LPWCYSHVG E+AL+H+ S FLK+ +D C HNLEALLHL+DGY GKG+K R+ R++ALVNKS FLKD+L IPPNWW T+ NR +EN TG
Sbjct: 1 MAIPLSKMGMPIKRGHSGTFRSKKPITTPNSDRKWT--SLGLSRRGDTTRMP-IISGVASRNDSTIAVSDLEKEKKNGYLGGEELIAETPNRRLADSWEEIHGQHDWDGMLDPIDPLLRSELIRYGEMAQACYDAFDSDPYSKYCGSCKTEPSKFFQYLGYKQYGYEVTRYNYASYNVNLPFLFKKTLSPNGWRPSANWIGYVAVSNDETSAYLGRRDITIAWRGTETKLEWIADFTDYLKSVSHEKIPSPDSRVKAEAGFIQVYTDKDITCKFCKYSAREQILAEVKRLIQKYADEELSITITGHSLGSALATINAYDIAETGVDVMDNGRVAPICVYSFSGPRVGNDRFKERLEELGVKVLRVRNVHDKVPKVPGIFFNEQVPPMMQQVGEMLPWCYSHVGVEIALNHKWSHFLKEKSDLACYHNLEALLHLLDGYRGKGQKIVRTDKRDLALVNKSCGFLKDHLLIPPNWWQTE----NRGLVENPTG 484
Match: A0A2K2BDB4 (Lipase_3 domain-containing protein {ECO:0000259|Pfam:PF01764})
HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 261/425 (61.41%), Postives = 326/425 (76.71%), Query Frame = 0
Query: 37 VSSRVRRCNMSTMVKSSQSEN-NMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRN 460
V S+ ST+ K + ++ N E + LA+ W+EI G++DW +LDP++PL++SELIRYG+MAQACYDAFD DP SKYCG C+F FFE LG+++ GY+VT YLY NIN P F+ KS WP WS+ ANW GYVAVSNDET+ LGRRDIT+AWRGTV + EW+AD+MDFL+PV +I DP+++VE+GFL LYTDKDE C+F +YSAREQI+AE+ RL + Y +EEMSITITGHSLG ALA+LSAYDI E G+ + +D R +P+ VFSFSGPRVGN RFK+R+E LGVKVLRVVN+ D VP PG+FFNE V P++ K+ E LPWCYSHVG ELALDH+NSPFLK+T DP C HNLEALLHL+DGYHGKG++F ++GR+ ALVNK+ DFLKD+ +PPNW +N RN
Sbjct: 56 VLSKASESLTSTITKHEKEQDYNSNTNTKEPERKLADEWREIQGKDDWVGLLDPMDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFMSHRFFESLGMTRHGYEVTRYLYGTSNINLPNFFKKSRWPKVWSNLANWIGYVAVSNDETTKRLGRRDITVAWRGTVTRLEWIADLMDFLKPVNGNKIPCPDPTVKVESGFLDLYTDKDENCRFCKYSAREQILAEVKRLTEMYADEEMSITITGHSLGGALAMLSAYDIVETGLHVMQDSRALPVSVFSFSGPRVGNVRFKKRIESLGVKVLRVVNVQDVVPKSPGLFFNEQVPPMLMKLTEGLPWCYSHVGVELALDHKNSPFLKQTGDPVCAHNLEALLHLLDGYHGKGQRFVLASGRDPALVNKACDFLKDHYLVPPNWRQDENKGMVRN 480
Match: A0A6A1V8S6 ((Phospholipase A1-Igamma2, chloroplastic {ECO:0000313|EMBL:KAB1208855.1}))
HSP 1 Score: 563.148 bits (1450), Expect = 0.000e+0
Identity = 268/470 (57.02%), Postives = 337/470 (71.70%), Query Frame = 0
Query: 21 VCQSQNSVAFGGGSFAVSSRVRRC--------NMSTMVKSSQSENNMTVVIGEKAK-------SLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQN-----------IEPNRNNMEN 464
V S G +FA + C ++ST++ + E V E+ K LA+ W+EIHG++DW MLDP+ PL++SELIRYG+MAQA YDAFD DP SKYCG C+F FF+ LG+S GYDVT YL++ NIN P F+ KS WP WS +ANW GYVAVSNDETS LGRRDITIAWRGTV + EW+AD+MDFL+P++ +I DP+++VEAGFL LYTDKD+ C+F +YSAREQI++E+ RL+ Y +EE+SITITGHSLG+ALAILSAYDI E G+++ D R +P+CVFSF+GPRVGN RFKERLE LG+KVLRVVN+HD VP PG+FFNE V P+M K+ E LPW YSHVG ELALDH+NSPFLK+T DP C HNLEA LHL+DGYHGKG +F ++GR+ ALVNK+ DFLKD+ +PP W +N I+P R +++
Sbjct: 31 VPVSGQKTQLGVSNFATKTPTVLCKVLSRTNDSLSTIITELEKERTQKVEQDEEPKISTQTESQLADLWREIHGQDDWVGMLDPMNPLLRSELIRYGEMAQASYDAFDFDPFSKYCGSCRFMRREFFDSLGMSGLGYDVTRYLFATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDETSKRLGRRDITIAWRGTVTRLEWIADLMDFLKPISSNKIPCPDPTVKVEAGFLDLYTDKDKSCRFCQYSAREQILSEVKRLLDMYVDEELSITITGHSLGAALAILSAYDIVETGLNVLTDSRAVPVCVFSFAGPRVGNVRFKERLETLGLKVLRVVNVHDVVPKSPGLFFNEQVPPMMMKLAEGLPWSYSHVGVELALDHKNSPFLKQTGDPSCAHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKACDFLKDHYLVPPYWRQDENKGMVMSKDGRWIQPERPRLDD 500
Match: A0A5C7I5E8 (SWIM-type domain-containing protein {ECO:0000259|PROSITE:PS50966})
HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0
Identity = 254/404 (62.87%), Postives = 322/404 (79.70%), Query Frame = 0
Query: 51 KSSQSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQN 454
+ +Q E+ T + + ++LA+ W+EIHG++DW MLDP++PL++SELIRYG+MAQACYDAFD DP SKYCG C+F FF LG+S GYDV+ YLY+ NIN P F+ KS WP WS +ANW GYVAVSNDET+ LGRRDITI+WRGTV + EW+AD+MDFL+P++ +I DP+++VE GFL LYTDKD C+F ++SAREQI++E+ RL++ Y +EE+SITITGHSLG+ALAILSAYDIAE G+++ KD R +P+ V SFSGPRVGN RFKER+E LGVKVLRVVN+HD VP PG+FFNE+VSP + K+ E LPW YSHVG ELALDH NSPFLK+T DP C HNLEAL+HL+DGYHGKG +F ++GR+ ALVNK++DFLKD+ +PP W +N
Sbjct: 503 RETQEEHLSTSRLHQSERNLADVWREIHGQDDWVGMLDPMDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFMHRKFFSSLGMSDHGYDVSRYLYATSNINLPNFFKKSRWPKIWSKNANWIGYVAVSNDETTKRLGRRDITISWRGTVTRLEWIADLMDFLKPISVNKIPCPDPTVKVEYGFLDLYTDKDVTCRFCKFSAREQIMSEVKRLLEMYSDEELSITITGHSLGAALAILSAYDIAETGVNVMKDSRAVPVSVVSFSGPRVGNVRFKERIEMLGVKVLRVVNMHDMVPKSPGLFFNENVSPFLMKLAEGLPWSYSHVGVELALDHTNSPFLKETGDPVCAHNLEALMHLIDGYHGKGHRFVLASGRDPALVNKASDFLKDHYLVPPFWRQDEN 906
Match: A0A200R0I9 ((Lipase {ECO:0000313|EMBL:OVA16237.1}))
HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0
Identity = 261/427 (61.12%), Postives = 325/427 (76.11%), Query Frame = 0
Query: 54 QSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQN-----------IEPNRNNMENVTGET 469
Q ++ + + + LA+TW+EIHG++DW +LDP++PL+++ELIRYGDMAQACYDAFD DP SKYCG C+F P F LG+ FGYDV+ YLY+ NIN P F+ KS W WS +ANW GYVAVSNDETS LGRRDITIAWRGTV + EW+ D+MD+L+PV+ +I D +++VE+GFL LYTDKD C+F +YSAREQI+ E+ RLIQKY +EE+SITITGHSLGSALAILS+YD+ E G+++ DGR +P+ VFSFSGPRVGN RFK RLE LGVKVLRVVN+HD VP +PGIFFNEHV PI++K+ E PW YSHVG ELALDH+NSPFLK TNDP C HNLEA LHL+DGYHGKG +F ++GR+ ALVNK++DFLKD+ +PP W +N I+P R ++ ET
Sbjct: 90 QEGSSSSTTDNQPKRKLADTWREIHGQDDWVGILDPMDPLLRTELIRYGDMAQACYDAFDYDPFSKYCGSCRFVPQNFSLSLGMKDFGYDVSRYLYATSNINLPNFFKKSRWSQVWSRNANWIGYVAVSNDETSKRLGRRDITIAWRGTVTRLEWIVDLMDYLKPVSDDKIPCPDQTVKVESGFLDLYTDKDGSCRFGKYSAREQILTEIRRLIQKYPDEELSITITGHSLGSALAILSSYDLVETGLNVMNDGRAVPVSVFSFSGPRVGNVRFKNRLESLGVKVLRVVNVHDTVPKVPGIFFNEHVPPIVQKMAEGTPWSYSHVGVELALDHKNSPFLKDTNDPTCSHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKASDFLKDHYLVPPFWRQDENKGMIRNHDGRWIQPERPKHDDHPPET 516
Match: A0A200Q4E4 ((Lipase {ECO:0000313|EMBL:OVA05340.1}))
HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0
Identity = 270/479 (56.37%), Postives = 352/479 (73.49%), Query Frame = 0
Query: 1 MAVPLSDVGWFFGSHSKE----KNVCQS----QNSVAFGGGSFA------VSSRVRRCNMSTMV----KSSQSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRNN 461
MA+ LS V FF H + K +S N +++ G VS+ ++ +++ + ++++ T V E+ K L + W+EIHG++DW +LDP++PL++SELIRYG+MAQACYDAFD DP SKYCG C++ FFE LG++ GY+V+ YLY+ NIN P F+ KS W WS +ANW GYV+VSNDETSA LGRRDITI+WRGTV + EW+AD+MD+LRPV +I DP+++VE+GFL +YTDKDE C+F +YSAREQI+ E+ RLIQ Y NEE+SIT+TGHSLG+ALA+LSAYDI E G++++ DGR +P+ VFSFSGPRVGN RFKERLE LGVKVLRVVN+HD VP +PGIFFNE V P ++K+ E PW YSHVG EL LDH+NSPFL+ T+DP C HNLEA LHL+DGYHGKGK+F +TGR+ ALVNK++DFLKD+ +PP+W +N RN+
Sbjct: 1 MAISLSKVSCFFTGHGYDTFSSKPTARSAQIFSNKLSYQGVGVTRKPLPKVSAVTDDNSLKSVIVELERETENQEEFTTV-EEQRKQLKDHWQEIHGKDDWIGLLDPMDPLLRSELIRYGEMAQACYDAFDYDPYSKYCGSCRYIRRKFFECLGMTDHGYEVSRYLYATTNINLPNFFKKSRWSKIWSRNANWIGYVSVSNDETSARLGRRDITISWRGTVTRLEWIADLMDYLRPVKSDKIPCPDPTVKVESGFLDVYTDKDENCRFCKYSAREQILTEIRRLIQMYPNEELSITLTGHSLGAALAVLSAYDIVETGVNVSTDGRAVPVSVFSFSGPRVGNRRFKERLEGLGVKVLRVVNVHDTVPKVPGIFFNERVPPFVQKLAEGFPWSYSHVGVELVLDHKNSPFLEDTSDPSCFHNLEAHLHLLDGYHGKGKRFVLATGRDPALVNKASDFLKDHYLVPPHWRQDENKGMVRNH 478
Match: A0A6P6V1M6 ((phospholipase A1-Igamma1, chloroplastic-like {ECO:0000313|RefSeq:XP_027095762.1}))
HSP 1 Score: 558.91 bits (1439), Expect = 0.000e+0
Identity = 258/406 (63.55%), Postives = 319/406 (78.57%), Query Frame = 0
Query: 57 NNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKY--ENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRN 460
N + IG K LA+ W+++HGENDW MLDP++PL+++ELIRYG+MAQACYDAFD DP S YCG C+F FFE+L + ++GYDVT YLY+ NIN P F+ +S WP WS +ANW GYVAVSNDETS LGRRDI IAWRGTV + EW+ADMMDFLRP++ +I DP+++VE+GFL LYTDKDE C+F ++SAREQI+ E+ RLI+ Y E EE+SI+ITGHSLGSALA LS YDI E GI++ +D R +P+CV+SFSGPRVGN RFK+R+E LG+KVLRVVN+HD VP PG+FFNE+V P++ K+ E LPW YSHVG ELALDH NSPFLK T DP C HNLEA LHL+DGYHGKG++F ++GR+IALVNK+ DFLKD+ IPPNW +N RN
Sbjct: 94 NQLAAEIGRK---LADCWRDMHGENDWVGMLDPMDPLLRTELIRYGEMAQACYDAFDFDPYSIYCGSCKFTRTKFFEELEMLQYGYDVTRYLYATSNINLPNFFKQSRWPKVWSKNANWIGYVAVSNDETSRKLGRRDICIAWRGTVTRLEWIADMMDFLRPISSDKIPCPDPTVKVESGFLDLYTDKDESCRFCKFSAREQILTEVKRLIEMYSDEEEELSISITGHSLGSALATLSGYDIVETGINVGRDSRGVPVCVYSFSGPRVGNVRFKQRVEGLGLKVLRVVNVHDVVPKSPGLFFNENVPPVVMKLAEGLPWSYSHVGVELALDHNNSPFLKPTTDPVCAHNLEAHLHLLDGYHGKGQRFVLASGRDIALVNKACDFLKDHHCIPPNWRQVENKGMVRN 496
Match: A0A314XVE1 ((Phospholipase A1-Igamma1 chloroplastic {ECO:0000313|EMBL:PQP96769.1}))
HSP 1 Score: 558.525 bits (1438), Expect = 0.000e+0
Identity = 254/447 (56.82%), Postives = 337/447 (75.39%), Query Frame = 0
Query: 34 SFAVSSRVRRCNMSTMVKSSQSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQN-----------IEPNRNNMENVTGET 469
S +++S + T+ K Q++ + + L++ W+E+HG++DW +LDP++PL++SELIRYG+MAQACYDAFD DP SKYCG C+F P FF LG+++ GY V+ YL++ NIN P F+ KS WP WS +ANW GYVAVS+DET+A LGRRDI+IAWRGTV + EW+ D+MDFL+P++ +I DP+++VE+GFL LYTDKDE C++S +SAREQI+ E+ RL++KY EE+SITITGHSLGSALAILSAYDI E G+++T DG+ +P+ V SF+GPRVGN RFKERLE LGVKVLRVVN+HD VP PG+FFNEHV+P + K+ E LPW YSHVG +L LDH+NSPFLK+T+DP C HNLEA LHL+DGYHGKG +F ++GR+ ALVNK++DFLKD+ +PP W +N I+P R +++ +T
Sbjct: 51 SDSLTSIITELEKETLNKDDQNQRQEDINTTHDERRLSDVWRELHGQDDWVGLLDPMDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFVPRSFFPSLGMAQHGYHVSRYLFATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSDDETTARLGRRDISIAWRGTVTRLEWIVDLMDFLKPISGNKIPCPDPTVKVESGFLDLYTDKDETCRYSTFSAREQILTEIKRLVEKYSGEELSITITGHSLGSALAILSAYDITETGLNVTADGQVVPVSVLSFAGPRVGNVRFKERLESLGVKVLRVVNVHDVVPKSPGLFFNEHVAPRLMKLAEGLPWSYSHVGVQLELDHKNSPFLKQTSDPVCAHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKASDFLKDHYLVPPYWRQDENKGMVRSKDGRWIQPERPKLDDHPEDT 497
| Match Name | Stats | Description |
|---|---|---|
| A0A1U8B4B0 | E-Value: 0.000e+0, PID: 65.74 | (phospholipase A1-Igamma1, chloroplastic-like isof... [more] |
| A0A835D6A5 | E-Value: 0.000e+0, PID: 65.74 | (Uncharacterized protein {ECO:0000313|EMBL:KAF8392... [more] |
| A0A4S4E7Z3 | E-Value: 0.000e+0, PID: 56.62 | Lipase_3 domain-containing protein {ECO:0000259|Pf... [more] |
| A0A2K2BDB4 | E-Value: 0.000e+0, PID: 61.41 | Lipase_3 domain-containing protein {ECO:0000259|Pf... [more] |
| A0A6A1V8S6 | E-Value: 0.000e+0, PID: 57.02 | (Phospholipase A1-Igamma2, chloroplastic {ECO:0000... [more] |
| A0A5C7I5E8 | E-Value: 0.000e+0, PID: 62.87 | SWIM-type domain-containing protein {ECO:0000259|P... [more] |
| A0A200R0I9 | E-Value: 0.000e+0, PID: 61.12 | (Lipase {ECO:0000313|EMBL:OVA16237.1})[more] |
| A0A200Q4E4 | E-Value: 0.000e+0, PID: 56.37 | (Lipase {ECO:0000313|EMBL:OVA05340.1})[more] |
| A0A6P6V1M6 | E-Value: 0.000e+0, PID: 63.55 | (phospholipase A1-Igamma1, chloroplastic-like {ECO... [more] |
| A0A314XVE1 | E-Value: 0.000e+0, PID: 56.82 | (Phospholipase A1-Igamma1 chloroplastic {ECO:00003... [more] |
Pages
back to top| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |