BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A7J7I6R1 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 1 MLLHASVATSRVPYRTRVKP-LKNSIVPSPSPS-----PSSQ----GPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAG-KQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGIVSILS-SPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTS------DWIC----------------------------RYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAI-DVDFKDD-SVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEGASHEASF 653
M + AS A S P R+ P L+ S+VP+PSP P + GP+A T L RHH HHH KP++ SP+PST PGC QIC EP TS P S C CVFPMKVRLL DVSL AIFPV+ ELEIE+AEGTYLKQSQV+I GASAD +NQ +T VDINLVPLGEKFDNT+A LTYERFWQKKVPLNKTLFG Y+V I YPGLPS P G +GPSGSAG +Q+PITADF++K KMN++TI II+ SA+V+LVVC GA+ ++L CRK GRPSNAV +TP+ N+R G SILS S SS S SL SAMPTS+LSVKTF+LAEL+KAT+KF++ RVLGEGGFGRVYHG++ED TE+AVKL+TR+ QNGDREFIAEVEMLSRLHHRNLVKLIGIC E HTRCL+YE+V NGSVESHLH DW RYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGR+PVDMSQP GQENLVTWARPLLTSREGLEQLVDP+L +Y FDDMAKVAAIA+MCVHPEVTHRPFMGEVVQALKLIYND D DC SQR SSA D DFK D SDSSWWNAGG TPRLTYG+A+S+ITM+YSSGPL++ NR FSGSS +G RV LPIRHGNRSGPLRTVRSKP+FYR KGS+SEH GLL KR W++G EASF
Sbjct: 101 MPMSASFAPSVSPRVPRLNPILEPSVVPAPSPLYQGLLPDVETPLLGPVAGPTTSNLPRHHRHHHHMKPISISPAPST-PGCGQICAEPFTSNPLDSPCGCVFPMKVRLLLDVSLYAIFPVVRELEIEIAEGTYLKQSQVIIIGASADSENQGRTVVDINLVPLGEKFDNTSAMLTYERFWQKKVPLNKTLFGNYEVMYINYPGLPSSPPFEGSMGNGPSGSAGSQQFPITADFVDKEPKMNSKTIFIIALSALVILVVCCGAISIILKCRKVGRPSNAVGPVFTPAANKRPGTGSILSCSNASSTSVSLISAMPTSILSVKTFSLAELEKATEKFSSKRVLGEGGFGRVYHGILEDRTEVAVKLLTRDIQNGDREFIAEVEMLSRLHHRNLVKLIGICVEDHTRCLVYELVRNGSVESHLHGADGRNGHLDWDVRLKIALGAARGLAYLHEDSNPRKMISPPRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMSQPPGQENLVTWARPLLTSREGLEQLVDPSLCRNYNFDDMAKVAAIASMCVHPEVTHRPFMGEVVQALKLIYNDADETCADCYSQRESSAAWDSDFKADIGPSDSSWWNAGGATPRLTYGHASSYITMDYSSGPLEDTENRPFSGSSLIGGRVFLPIRHGNRSGPLRTVRSKPAFYRLKGSVSEHGGLLGKRAWNDGTGFEASF 804
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A7J0HAT1 ((Protein kinase superfamily protein {ECO:0000313|EMBL:GFZ20179.1}))
Query: 17 RVKPLKNSIVPS--PSPSPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAG-KQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGI-VSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTSDWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDDSV-SDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEGAS-HEASF 653
R + S PS P+PSP S GP A T TL RHHHHHH+ KP++ +P+PSTVPGC QIC+EPLTS P GS C CVFPMKVRLL DVSL AIFPV+NELEIEVA GTYLKQSQV+I GASAD QNQ++T VD+NLVPLGEKFDNT+A LTYERFWQ+KVP+NKTLFG Y+V I+YPGLPS P G+ GPSGSAG +Q+PITADF+NK QKM+ R I +I+ SA+V+LVV GA+ + L R+ GRPSNAV +TPS N+R G + SS SS S SL S MPTS+LSVKTFTL EL+KATDKF+ RVLGEGGFGRVY G++ED TE+AVKL+TR +QNGDREFIAE+EMLSRLHHRNLVKLIGIC E HTRCL+YE+VPNGSVESHLH GHLLVKSDVYSYGVVLLELLSGR+PVDMSQP GQENLVTWAR LLTSREGL LVDP+LA Y FDDMAKVAAIA+MCVHPEVTHRPFMGEVVQALKLIYNDTD GDC SQ+ SSA D DFK D V SDSSWWNAGG++P LTYG+A+SFITMEYSSGPL+E+ NR FS S G LPIRHGNRSGPLRTVR KP+FYR KGSMSEH GLLSK W+ S EASF
Sbjct: 31 RSTSMSASFAPSVAPAPSPLSPGPAAGPATSTLPRHHHHHHRMKPISVAPAPSTVPGCGQICMEPLTSTPIGSPCGCVFPMKVRLLLDVSLYAIFPVVNELEIEVATGTYLKQSQVIIIGASADSQNQERTVVDMNLVPLGEKFDNTSAMLTYERFWQQKVPINKTLFGNYEVVYISYPGLPSSPPFEGYMGHGPSGSAGTRQFPITADFVNKRQKMSARLIFVIAFSAIVLLVVLCGAISIFLRWRRVGRPSNAVGPVFTPSTNKRPGTGSILSSSVASSTSVSLMSTMPTSILSVKTFTLVELEKATDKFSLKRVLGEGGFGRVYLGILEDRTEVAVKLLTRGNQNGDREFIAEIEMLSRLHHRNLVKLIGICVEDHTRCLVYELVPNGSVESHLH-----------------GHLLVKSDVYSYGVVLLELLSGRKPVDMSQPPGQENLVTWARSLLTSREGLALLVDPSLARKYDFDDMAKVAAIASMCVHPEVTHRPFMGEVVQALKLIYNDTDETCGDCCSQKESSARDSDFKGDLVPSDSSWWNAGGISPHLTYGHASSFITMEYSSGPLEEMENRPFSASGLTGGGGFLPIRHGNRSGPLRTVRIKPAFYRLKGSMSEHGGLLSKPVWNNSNSGFEASF 660
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A6A4M295 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011} (Fragment))
Query: 1 MLLHASVATSRVPYRTRVKPLKNSIVPS-PSPSPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAG-KQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGI-VSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLH-------------------------------VTSDWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDDSVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEGAS-HEASF 653
M + S + S P+ +KP +S PS P PSP +GP+A T T RHH HH +P++F+P+PST PGC QIC+EPLTS P GS C CVFPMK+RL+ DVSL IFP++N+LEI++A GTYL+QSQVVI GASAD QNQ +T VDINLVPLGEKFDNT+A LTYERFW+KKVPLNKTLFG Y+V SI+YPGLPS P G+ +GP+GSAG +QYPITADF+NKSQKMN R I IIS SA+VVLVVC GAV +LL RK GRPSNAV +TPS N+R G + SS SS S SL S MP S+LSVKTF+LAEL KAT+KF+ RVLGEGGFGRV+HG++ED TE+AVKL+TR++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E HTRCL+YE+V NGSVESHLH +++ + YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGR+PVDMSQ GQENLVTWARPLLT REGL QLVDP+LAG++ FDDMAKVAAIA+MCVHPEVTHRPFMGEVVQALKLIYND D GDC SQ+ SSA D DFK +SSWWNAGG++P ITMEYSSGPL+E+ NR FS S+ G LPI HGNRSGPLRTVRSKPSFYR KGS+SEH GLLSK W++G S EASF
Sbjct: 61 MSMSGSFSPSEAPHMPWLKP--SSEPPSIPGPSPLYRGPVAGPSTSTFPRHHRHHRHLEPISFTPAPSTTPGCGQICVEPLTSTPIGSPCGCVFPMKIRLVLDVSLYGIFPLVNQLEIDIAAGTYLRQSQVVIIGASADSQNQGRTVVDINLVPLGEKFDNTSALLTYERFWEKKVPLNKTLFGNYEVMSISYPGLPSSPPFDGYAGNGPTGSAGNQQYPITADFVNKSQKMNARLIFIISFSALVVLVVCCGAVSILLRWRKVGRPSNAVGPVFTPSTNKRPGTGSVLSSSMASSTSISLVSTMPFSILSVKTFSLAELDKATNKFSLKRVLGEGGFGRVFHGILEDRTEVAVKLLTRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICVEDHTRCLVYELVSNGSVESHLHELIFGNIAFCVQMTEIVKFLSSFTLYLVTPSLSALLVTEYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMSQSPGQENLVTWARPLLTCREGLAQLVDPSLAGNFDFDDMAKVAAIASMCVHPEVTHRPFMGEVVQALKLIYNDNDETCGDCCSQKESSAHDSDFK----GESSWWNAGGVSPH---------ITMEYSSGPLEEIENRPFSASTFTGGGGFLPINHGNRSGPLRTVRSKPSFYRLKGSVSEHGGLLSKGVWNDGTSGFEASF 737
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A6A6KX98 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 21 LKNSIVPSPSP--SPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGIVSILS-SPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLH-------VTSDWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDD-SVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEG 646
+K S+ P+ SP S+ GP + R H HHHH+ KP+ + SPS C Q+C EPLT+APFGS C+CVFPMKVRLL ++ AIFPV+N+LEIEVA GTYLKQSQV I GASAD QNQ KT VDINLVPLG+KFDNTTA LTY+RFW KKVPLN TLFG Y+V SI+YPG+PS P + SGPSG+AG PITA F++KSQKMN RTI I++ SA VVL+V IGAV + L RK GRPS+AV S+N+RSGI S++S S SS S SL S M T +LSVKTF AEL++AT+KF++ R+LGEGGFGRVYHG++EDGTE+AVKL+TR++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E TRCL+YE+VPNGSVESHLH I YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGR+PVDM QP G+ENLVTWARPLLTSREGLEQLVDP+LAG+Y FDDMAKVAAIA+MCVHPEVT+RPFMGEVVQALKLIYND D GD SQ+ SSA D DFK D + S SWWNAGG++PR+T+G A+SFITMEYSSGPL+E NR FS SS +GDR+SL I+ GNRSGPLRTVRSK +FYR +GSMSEH GLLSKR W+ G
Sbjct: 362 VKPSLAPALSPVYQASAFGPSNAPLPRHHRLHRHHHHRVKPVVVASSPSEDQSCGQVCSEPLTAAPFGSPCACVFPMKVRLLLGMAPYAIFPVMNQLEIEVAAGTYLKQSQVKIMGASADSQNQGKTVVDINLVPLGDKFDNTTAILTYDRFWHKKVPLNNTLFGDYEVISISYPGIPSSPPYSDYMGSGPSGTAG-DLPITAHFVSKSQKMNARTIAIVALSAFVVLLVFIGAVFIFLRWRKFGRPSSAVGPTIASSINKRSGIGSLMSTSIASSTSMSLVSTMATCMLSVKTFPYAELEQATEKFSSKRILGEGGFGRVYHGIMEDGTEVAVKLLTRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRTRCLVYELVPNGSVESHLHGMFHRNFEIYGVILWYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMFQPPGEENLVTWARPLLTSREGLEQLVDPSLAGTYGFDDMAKVAAIASMCVHPEVTNRPFMGEVVQALKLIYNDMDETCGDNCSQKESSAPDSDFKVDLAPSYGSWWNAGGISPRITHGQASSFITMEYSSGPLEETENRPFSASSLVGDRLSLAIKQGNRSGPLRTVRSKTAFYRLRGSMSEHGGLLSKRNWNGG 1001
Query: 1 MLLHASVATSRVPYRTRVKPLKNSIVPS--PSPSPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGIVSILSSPTSSKSAS-LFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTSDWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDD-SVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEHG-LLSKRGWDEG 646
+L+ S +S+ ++ L++S+ PS P+PSP QGPI S + R H HH + + +A +PS EPLT+ PFGS C CVFPMKVRLL DV+ A+FPV+NELEIEVA GTYL+QSQV I GASAD QNQ KT VDINLVPLGEKFDNTTA LTY+RF KKVPLN TLFG Y+V I+YPG+PS P G SGP+ SAG PITA+F NK+QKMN RTI II+ SA V+L+V +GAV + + RK G+PS V A+T S+++RSGI SILSS +S ++ L S M S+LSV+TF+L+EL+KATDKF++ R+LGEGGFGRVY GL+EDGTE AVKL+TR++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E RCL+YE+VPNGSVESHLH T YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGR+PVDMSQP GQENLVTWARPLLT+REGLEQLVDP LAG FDDMAKVAAIA+MCVHPEVTHRPFMGEVVQALKLIYNDTD GDC SQ+ SSA D DFK D +VSD SWWNAGG++PRLTYG A+SFITMEYSSG L+E+ NR FS SS +GD +SLP+RHGNRSGPLRTVRSKP+FYR GS SEHG L +R W++G
Sbjct: 143 ILMSLSFGSSKF---SKTTVLESSLAPSTAPAPSPIYQGPIGSLPPRHIHRRHRHHARPRAVAPAPSKDQ---------EPLTATPFGSPCGCVFPMKVRLLLDVAPFAVFPVMNELEIEVAAGTYLEQSQVKIMGASADSQNQGKTIVDINLVPLGEKFDNTTAILTYQRFRHKKVPLNMTLFGNYEVIYISYPGIPSSPPYGSFMGSGPTASAG-NLPITANFGNKNQKMNVRTIAIIALSAFVLLMVLLGAVSIFIKWRKVGKPSGVVGPAFTSSIHKRSGIGSILSSSIASSTSVSLISTMAPSILSVRTFSLSELEKATDKFSSQRILGEGGFGRVYSGLMEDGTEAAVKLLTRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRKRCLVYELVPNGSVESHLHGT------YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMSQPQGQENLVTWARPLLTNREGLEQLVDPALAGCCDFDDMAKVAAIASMCVHPEVTHRPFMGEVVQALKLIYNDTDETCGDCCSQKESSAPDSDFKGDLAVSDGSWWNAGGISPRLTYGQASSFITMEYSSGQLEEMDNRPFSTSSLIGDEISLPVRHGNRSGPLRTVRSKPAFYRLTGSRSEHGAFLPRRAWNDG 777
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A5N5K8I4 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 3 LHASVATSRVPYRTRVKPLKNSIVPSPSPSPS-------SQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGI-VSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTSDWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDDSV-SDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEG 646
+ S A SR PY++ V + S+ P+ SP+PS P A+ H HHH KP+ +PSPS C+QIC EPLT+ PFGS C CVFPMKVRLL DV+ A+FPV+ ELE EVA GTYL++SQV I GASA +QNQ KT VDINLVPLGEKFDNTTA LTY+RFW+KKVPLN TLFG Y+V I+YPG+PS + SGPSGS G PITA F+NK+Q+MN RTI II+ SA VVLVV GA+ +++ RK GRPS AV A+T S+N+RSGI + SS SS S SL S M T +LSVKTFT EL+KATDKF++ R+LGEGGFGRV+ G +EDG E+AVKL+ R++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E RCL+ YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGR+PVDMSQP GQENLVTWARPLLTSREGLE LVDP+LAGSY FDDMAKVAAIA+MCVH EVT+RPFMGEVVQALKLIYNDTD GD SSQ+ SS ++ DFK D V SDSSWWNAGG +PRLTYG A+SFITM+YSSGPL+E+ NR FS S GD +SLPIRHGNRSGPLRTVRSKP+FYR++GSMSEH GLLS+R W++G
Sbjct: 90 MSVSFARSR-PYQSPVA--QPSLGPTISPAPSLVHQDFLPVPPSATLPRRHGGHHSHHHRHVKPVVTAPSPSDEQSCDQICTEPLTTVPFGSPCGCVFPMKVRLLLDVAPYAVFPVMRELESEVAAGTYLEESQVKIMGASAANQNQGKTVVDINLVPLGEKFDNTTAILTYDRFWKKKVPLNITLFGNYEVIYISYPGIPSSSPYPNYTWSGPSGSTG-DLPITAHFVNKNQRMNLRTIAIITLSAFVVLVVFTGAIAIVIRWRKSGRPSIAVGPAFTSSINKRSGIGSFLSSSVASSTSISLMSNMATCMLSVKTFTFTELEKATDKFSSKRILGEGGFGRVFSGSMEDGAEVAVKLLMRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRARCLV----------------------YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMSQPPGQENLVTWARPLLTSREGLETLVDPSLAGSYDFDDMAKVAAIASMCVHSEVTNRPFMGEVVQALKLIYNDTDETCGDYSSQKGSSGLESDFKGDLVPSDSSWWNAGGTSPRLTYGQASSFITMQYSSGPLEEMENRPFSTLSLAGDGLSLPIRHGNRSGPLRTVRSKPAFYRSRGSMSEHGGLLSRRFWNDG 720
Query: 4 HASVATSRVPYRTRVKP-----LKNSIVPSPSPSPS--SQGPIASHRTGTL-----RRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGI-VSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTS------DWICRYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDDSV-SDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEG 646
H +A RV + R +P ++ S+ P+ SP+PS Q + R+ TL H HHH KP+ +PSPS C+QIC EPLT+ PFGS C CVFPMKVRLL DV+ A+FPV+ ELE EVA GTYL++SQV I GASA +QNQ KT VDINLVPLGEKFDNTTA LTY+RFW+KKVP+N TLFG Y+V I+YPG+PS + SGPSGS G PITA+F+NK+Q+MN RTI IIS S VVLVV IGA+ +++ RK GRPS+AV A+T S+N+RSGI + SS SS S SL S M T +LSVKTFT EL+KATDKF++ R+LGEGGFGRV+ G +EDG E+AVKL+ R++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E TRCL+YE+V NGSVESHLH DW R A G + D + P + R+ A SY FDDMAKVAAIA+MCVH EVT+RPFMGEVVQALKLIYNDTD GD SSQ+ SS ++ DFK D V SDSSWWNAGG +PRLTYG A+SFITM+YSSGPL+E+ NR FS S +GDR+SLPIRHGNRSGPL+TVRSKP+FYR++GSMSEH GLLS+R W++G
Sbjct: 56 HGMLAAMRVSF-ARSQPSQSPAVQPSLGPTLSPAPSLVHQDFLPVPRSATLPRRHGGHHSHHHRHVKPVVTAPSPSDEQSCDQICTEPLTTVPFGSPCGCVFPMKVRLLLDVAPYAVFPVMRELESEVAAGTYLEESQVKIMGASAANQNQGKTVVDINLVPLGEKFDNTTAILTYDRFWKKKVPINITLFGDYEVIYISYPGIPSSSPYPNYTGSGPSGSTG-DLPITANFVNKNQRMNLRTIAIISLSGFVVLVVFIGAIAIVIRWRKSGRPSSAVGPAFTSSINKRSGIGSFLSSSVASSTSISLMSNMATCMLSVKTFTFTELEKATDKFSSKRILGEGGFGRVFSGSMEDGAEVAVKLLMRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRTRCLVYELVRNGSVESHLHGVDKNKGPLDWDARLKIALGAARGLAYLHED--------------------------------SNPRVIHRD--------FKARSYDFDDMAKVAAIASMCVHSEVTNRPFMGEVVQALKLIYNDTDETCGDYSSQKGSSGLESDFKGDLVPSDSSWWNAGGTSPRLTYGQASSFITMQYSSGPLEEMENRPFSSLSLVGDRLSLPIRHGNRSGPLKTVRSKPAFYRSRGSMSEHGGLLSRRFWNDG 680
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A5N6QF23 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 63 SPSPSTV-PGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRR-SGIVSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLITRESQNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTSDWICRY---VAPEYAMTGHLLVKS-----DVYSYGVVLLELLSGRQPVD--------------------------MSQPSGQENLVTWARPLLTSR-EGLEQLV-----------------DPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDD-SVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEH-GLLSKRGWDEG 646
+P+PS V C QIC+EPLT+ PFGS C CVFPMKVRLL DV+ A+FPV++ELEIEVA GTYL+QSQV I GASAD QNQ++T VDINLVPLGEKFDNTTA LTYERFW KKVPLN+TLFG Y++ I YPG+PS P G SGPSGSAG PI+A+F K+Q+MN +TI II+ SA V+L+V IGAV + L RK GRPS+AV A T N+R + SS SS S SL S M T +LS KTF+LAEL+KATDKF++ R+LGEGGFGRVY G++ED TE+AVKL+TR++QNGDREFIAEVEMLSRLHHRNLVKLIGIC E TRCL+YE++ NGSVESHLH TS + + + P G L V+ D + VL+E G + S L+T +P+ S+ +G E LV DP+LAGSY FDDMAKVAAIA+MCVHPEVT RPFMGEVVQALKLIYNDTD GD SQ+ SSA D DFK D + SDSSWWNAGGLTPRLTYG+A+SFITM+YSSG L+++ NR FS S+ +G +SLPIRHGNRSGPLRTVRSK +FYR GS SEH GLL KR W++G
Sbjct: 34 APAPSPVYQACGQICVEPLTATPFGSPCGCVFPMKVRLLLDVAPYAVFPVMSELEIEVAAGTYLEQSQVKIMGASADSQNQERTVVDINLVPLGEKFDNTTAVLTYERFWHKKVPLNQTLFGNYELVYINYPGIPSSPPHGSDIGSGPSGSAG-DLPISANFDKKNQRMNLKTIAIIALSAFVLLLVFIGAVSIFLKWRKAGRPSSAVGPALTSPTNKRSGIGSILSSSIASSTSVSLMSTMATRVLSAKTFSLAELEKATDKFSSKRILGEGGFGRVYFGIMEDRTEVAVKLLTRDNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRTRCLVYELIHNGSVESHLHATSKSLEQKSVDIDP-ILFVGILFVEMNQLPIDYFHITSVLVEATEGSHHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDMSQPQGQENLVTWARPLLTSREGLEQLVDPSLAGSYDFDDMAKVAAIASMCVHPEVTQRPFMGEVVQALKLIYNDTDETCGDYCSQKESSARDSDFKGDFAPSDSSWWNAGGLTPRLTYGHASSFITMDYSSGQLEDMENRPFSASTLVGGEMSLPIRHGNRSGPLRTVRSKAAFYRFSGSKSEHGGLLPKRAWNDG 674
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A830BCN6 ((Receptor-like serine/threonine-protein kinase ale2 {ECO:0000313|EMBL:GFP82999.1}))
Query: 12 VPYRTRVKPLKNSIVPSPSPSPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAG-KQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGIVSILSSPTSSKSASLFSAMPTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVEDGTEIAVKLI-TRES-QNGDREFIAEVEMLSRLHHRNLVKLIGICFERHTRCLIYEVVPNGSVESHLHVTS------DWICRY------------------------------------VAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTDGDCS---SQRVSSAIDVDFKDDSV-SDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEHG-LLSKRGWDEGASHEASF 653
V R R K L S P+P+P+P SQGP+ T R+H HH H+ + SPSPST P +L AIFP + ELEIEVA GTYLKQSQV I GASAD NQ++T VDINLVPLGEKFD+TTA LT++RF KKVPLN+TLFG Y V +I+YPGLPS P SG GP+GSAG +QYPITADF+ K+QK+N R I++I+ SA V+LVV V +L+ CR RPSN V EL+ AT+KF++ +VLGEGGFGRVY G++ DGTE+AVKL+ TR++ QNGDREFIAE+EMLSRLHHRNLVKLIGIC E+ TRCL+YE+VPNGSVESHLH DW R P+ + GHLLVKSDVYSYGVVLLELLSGR+PVDMSQ GQENLVTWARPLLT+REGLE LVDP+LAG+Y FDDMAKVAAIA+MCVHPEVTHRPFMGEVVQALKLIYNDTD C+ S + SSA D DFK D SD SWWNAG +TPRLTYG A+SFITMEYSSGPL+E+ NR FS S V+LPIRHGNRSGPLRTVRSK +FYR +GS+SEHG + ++R W++G +EASF
Sbjct: 86 VARRHRNKYL--STAPAPAPTPVSQGPL----TSRTRKHRHHRHKARAHVVSPSPSTNP---------------------------------ALYAIFPEVTELEIEVAAGTYLKQSQVAIIGASADALNQERTVVDINLVPLGEKFDDTTAILTFQRFLHKKVPLNRTLFGDYGVMNISYPGLPSSPPSGILFGEGPTGSAGNQQYPITADFVGKNQKINPRIIIVIALSAFVLLVVACAVVAILVKCRTASRPSNVV----------------------------------------------ELENATEKFSSKKVLGEGGFGRVYLGVLHDGTEVAVKLLLTRDNNQNGDREFIAEIEMLSRLHHRNLVKLIGICIEQRTRCLVYELVPNGSVESHLHGDDKSEGPLDWDARLKIALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGHLLVKSDVYSYGVVLLELLSGRKPVDMSQSPGQENLVTWARPLLTNREGLEHLVDPSLAGTYNFDDMAKVAAIASMCVHPEVTHRPFMGEVVQALKLIYNDTDETCADAFSLKESSARDSDFKGDFAHSDGSWWNAGCVTPRLTYGQASSFITMEYSSGPLEEMENRPFSASCLGEGGVALPIRHGNRSGPLRTVRSKRAFYRLQGSVSEHGDIFTRRAWNDGTDYEASF 692
BLAST of DCAR_000551 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: G7KZH8 ((Receptor-like Serine/Threonine-kinase ALE2 {ECO:0000313|EMBL:AES81663.2}))
Query: 18 VKPLKNSIVPSPSPSPSSQGPIASHRTGTLRRHHHHHHQTKPLAFSPSPSTVPGCNQICLEPLTSAPFGSLCSCVFPMKVRLLFDVSLLAIFPVINELEIEVAEGTYLKQSQVVITGASADDQNQDKTAVDINLVPLGEKFDNTTATLTYERFWQKKVPLNKTLFGTYDVASITYPGLPSLPLSGGHHSSGPSGSAGKQYPITADFINKSQKMNTRTILIISSSAVVVLVVCIGAVIVLLNCRKDGRPSNAVRHAYTPSVNRRSGIVSILSSPTSSKSASLFSAM-PTSLLSVKTFTLAELQKATDKFNANRVLGEGGFGRVYHGLVE----DGTEIA-VKLITR--------------------------------------ESQNGDREFIAEVEM-------LSRLHHRNLVKLIGICFERHTRCLIYEVVPNGS--------VESHLHVTSDWICR--YVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRQPVDMSQPSGQENLVTWARPLLTSREGLEQLVDPTLAGSYQFDDMAKVAAIANMCVHPEVTHRPFMGEVVQALKLIYNDTD---GDCSSQRVSSAIDVDFKDD-SVSDSSWWNAGGLTPRLTYGNATSFITMEYSSGPLDELGNRAFSGSSPMGDRVSLPIRHGNRSGPLRTVRSKPSFYRTKGSMSEHGLL-SKRGW 643
VKP ++ +P PSPS QGP + R H HH P A +P PST P C+QIC +PLTS PFGS C CVFPMKVRL DV+ A+FPV+NELE EVA GTYL+QSQV I GA+AD QNQ +T+VDINLVPLGEKFDNTTA LTYERFW KKVPLN++LFG Y V ITYPGLPS SG SGPS + P +A+F +KSQK N RTI+II+ S+ V+L+V IGA V L RK RPS+AV A+T S+N+RSG+ S+LSS +S ++ + PTS+LSVKTF+L+E++KATDKFN RVLGEGGFGRVY G +E D IA V++++R + G ++ A +++ L+ LH + ++I F+ L + P S E H+++ + YVAPEYAMTGHLLVKSDVYSYGVVLLELL+GR+PVDMSQP GQENLVTWAR LLTSREGLEQLVDP+LAG Y FDDMAKVAAIA+MCVH EVT RPFMGEVVQALKLIYNDTD GD SQ+ SSA + DF+ + + SDSSWWN GGLTPRLTYG A+SFITMEYSSGPL+++ NR FS SS GD +SLPIRHGNRSGPLRT RSK S YR GS SEHG + SKR W
Sbjct: 40 VKP-SSAPTSAPVPSPSYQGPSVTPRHKHHHHHRRHHSTRPPYAGAPPPSTDPACDQICTDPLTSTPFGSPCGCVFPMKVRLTLDVAPFAVFPVLNELEYEVALGTYLEQSQVKIMGATADSQNQGRTSVDINLVPLGEKFDNTTAALTYERFWHKKVPLNRSLFGDYAVMYITYPGLPSSLPSGTPIGSGPSQNDDGILPFSANFDSKSQKTNLRTIVIIALSSFVLLLVLIGAFFVTLKWRKTRRPSSAVGPAFTSSLNKRSGLGSMLSSSIASSTSMSLMSTMPTSILSVKTFSLSEIEKATDKFNTKRVLGEGGFGRVYSGTLEDGNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRRRCLVYELVPNGSVESHLHGDDKNRGPLDWEARMKIALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREATEGSNHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDMSQPQGQENLVTWARALLTSREGLEQLVDPSLAGGYNFDDMAKVAAIASMCVHSEVTQRPFMGEVVQALKLIYNDTDETGGDYCSQKDSSAQESDFRGELAPSDSSWWNGGGLTPRLTYGQASSFITMEYSSGPLEDMENRPFSTSSFNGDELSLPIRHGNRSGPLRTTRSKLSLYRFSGSRSEHGEVSSKRNW 730
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.
There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.
LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.
Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.
RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.
The NCBI Sequence report lists the correspondences between the various naming methods
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.
