BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A6P6TVC6 ((U-box domain-containing protein 35-like {ECO:0000313|RefSeq:XP_027082283.1}))
Query: 12 DKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSL----PTLRFNSSPDVSPSSEANVLNNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSFRSPFT-RKGPNGRPYELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTP-RLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMS--QASYP---QSGFSSTGN 798
+KLIAVAID+D+GSQ A+KW VD +L +GQT+LLVHVK K SS SS S+ P NSS + + +D+FLPFRVFCTRKDIHCQDV++EDTDVVR L +YV+ V++LVLGA +KG +L RFK +DIP V KGVPDFC+VYIISKGKI +TR+AS AP + PLR Q+ Q+ S S P S N++ ++ +SPFT RKGPNG+PYELS+P+ DISF SSGRPS + + +F DS + TP R+S FS + +GRRSVD T ++SV D + S++ DD++ EMRRLKLELKQTM+MYSTACKEALSAKQKAMEL RWK EE+QRLEEAR+AEETALALAE+EK +S EHAEAA+R+AE E QKRINAE K L+ EE NK + QS RYR YTIDEIE AT++F KS KIGEGGYGPV+KC LDHT VAVKVLRPDAA GRSQFQQEVE+LSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRL ++GN+ L WQHRFRIAAEI GLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP +V + VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD+YSLGI++LQ++T K PMG+TH VE AIEKGTF+E++DP++PDWPIEEAL+ AKI+L+C+ELRRKDRPDLG V++PELERLR F E+ M+ ++ Y +G SP SQ ++S + SYP Q F S+ N
Sbjct: 5 NKLIAVAIDKDRGSQNALKWTVDNLLVRGQTILLVHVKAKLHGSSFSSSSVALPRPNQVTNSSDEYGMYGRDYDVETKDIFLPFRVFCTRKDIHCQDVILEDTDVVRALLEYVSRIGVDVLVLGAAAKG-YLFRFKARDIPASVLKGVPDFCSVYIISKGKIVATRSASCSAPVNHPLRQQMLDQASSQLSSTRQASEPSSR--------------------------NIKTDSTTMKSPFTHRKGPNGKPYELSSPDTDISFVSSGRPSIDNTFPSFADSINSGPTPPRISGFSEMENRSFESLHFGRRSVD-------------------IITSPEFSSVFGDNDASMAQATDDMEVEMRRLKLELKQTMEMYSTACKEALSAKQKAMELHRWKMEEQQRLEEARLAEETALALAEKEKTKSGVVAEHAEAAQRIAELETQKRINAEMKTLKEMEENNKLFNRLVQSNIRYRVYTIDEIEIATQYFDKSLKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAHGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLFRRGNTPALPWQHRFRIAAEITIGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIIFLQILTGKPPMGLTHQVERAIEKGTFTEMVDPAIPDWPIEEALNLAKIALKCSELRRKDRPDLGKVVMPELERLRAFGEEKMSFSLTYSSGQSPMQSQVSLSTDELSYPPTEQCSFESSKN 759
Query: 1 MLGHAERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSLPTL-RFNSSPDVS---------PSSEANVLNNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSF-RSPFT-RKGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPA-DDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQ--ASYP---QSGFSST 796
M + ERN A ++++AVAIDRDKGSQ A+KWA+D IL++GQTV+LVHVK+K ASS+ S + + RF+ DV P S+ +++FLPFR FCTRKDI C+DV +EDTDV L ++VT SA+EILVLGA SK FL RFK DIPG VSKGVPDFCTVY+ISKGKIS TR+ASR APA SPLRN+I +Q+ S RSE P P PP+ S+ G L ++ P D F +SPFT RK NG+P+ ELS P+ DISF SSGR S +R++ +F DS +TN +PRLS++S+ L+ + S+ GR S+DT TP E ++ V D + S S A DD++AEM+RLKLELKQTM+MYSTACKEAL+AKQKA ELQ WK EEEQRL EAR+AEE ALA+ EREKA+S+AAIEHAEAA+R+AE E+QKRI+AE KA + EE+NK +AQS+ RYRKYTI+EIE AT FF++SRKIGEGGYGPV+KC LDHT VAVKVLRPDAAQGRSQFQQEVE+LSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRL ++GN+ PLSWQ+RFRIAAEI TGLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP +V D+VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD+YS+GIM+LQ++TAK PMG+TH VE AIEKGTF+E+LD +VPDWP+E+ALSFAKI+L+CAELRRKDRPDLG V++PELERLR AE+NM M+ G+ SP +SQ +M Q SYP QSG+ S+
Sbjct: 3 MPRNGERNEA-NRMVAVAIDRDKGSQVALKWAIDNILNRGQTVVLVHVKVKPSASSHYSSTSLSHPRFSQISDVGGEIGSVYGEPDSQ-----TKELFLPFRCFCTRKDIQCKDVALEDTDVTNALVEFVTQSAIEILVLGAPSKNGFLRRFKATDIPGSVSKGVPDFCTVYVISKGKISLTRSASRPAPAVSPLRNEIMNQA-SRRSEQSETPLP--------PPI-----SMKGVPRLHSEPTHNTPNDLDFIKSPFTHRKAANGKPHAELSPPDTDISFVSSGRTSIDRIFPSFYDSLDTNRSPRLSSYSD-------LDNHSFESLHF----------GRKSIDTATPPE--FSQVSHDSDKSSSSQAMDDVEAEMKRLKLELKQTMEMYSTACKEALTAKQKAKELQHWKMEEEQRLGEARLAEEAALAIVEREKAKSRAAIEHAEAAQRIAELESQKRIHAEMKAHKEAEEKNKMLNKLAQSDIRYRKYTIEEIEAATNFFSESRKIGEGGYGPVYKCYLDHTQVAVKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLCRRGNTPPLSWQNRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSVGIMFLQILTAKPPMGLTHHVERAIEKGTFAEMLDQAVPDWPVEKALSFAKIALKCAELRRKDRPDLGKVVMPELERLRALAEENM---MMDGSIHSPNHSQVSMCQDELSYPTTAQSGYDSS 778
Query: 1 MLGHAERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSLPTL-RFNSSPDVSPSSEANVL-----NNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSF-RSPFT-RKGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPA-DDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQ--ASYP---QSGFSST 796
M + E+N A ++++AVAIDRDKGSQ A+KWA+D IL++GQTV+LVHVK+K ASS S + RFN DVS +V +++FLPFR FCTRKDI C+DV +EDTDV L ++VT SA+EILVLGA SK FL RFK DIPG VSKGVPDFCTVY+ISKG+IS T++ASR APA SPLRN+I Q+ S RSE P P + + P M HS P + P D F +SPFT RK N +P+ ELS P+ DISF +SGR S +RM+ +F DSF+TN +PRLS++S+ L+ + S+ GR S+DT TP+E ++ V D + S S A DD +AEMRRLKLELKQTM+MYSTACKEAL+AKQK ELQ WK EEEQRLEEAR+AEE ALA+ EREKA+S+AAIEHAEAA+R+AE E+QKRINAE KA + EE+NK +A+S+ RYRKY I+EIE AT F++SRKIGEGGYGPV+KC LDHT VAVKVLRPDAAQGRSQFQQEVE+LSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRL ++GN+ PLSWQHRFRIAAEI TGLLFLHQTKPEPLVHRDLKPGNILLDRN+VSKISDVGLARLVP ++ D+VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD+YS+GIM+LQ++TAK PMG+TH +E AIEKGTF+E+LDP+VPDWP+EEALSFAKI+L+CAELRRKDRPDLG V++PELERLR AE+NM M G+ SP +SQ +M Q SYP QSG+ S+
Sbjct: 3 MPRNGEKNEA-NRMVAVAIDRDKGSQVALKWAIDNILNRGQTVVLVHVKVKPSASSYYSSPSLSHPRFNQISDVS-GKIGSVYGEPDPQTKELFLPFRCFCTRKDIQCKDVALEDTDVTNALVEFVTQSAIEILVLGAPSKNGFLRRFKATDIPGSVSKGVPDFCTVYVISKGRISLTQSASRPAPAVSPLRNEIMYQA-SRRSEQSETPPPPAISMKGVPRM----HSEPTHN---------MPNDLDFIKSPFTHRKAANSKPHAELSPPDTDISFVNSGRTSVDRMFPSFYDSFDTNRSPRLSSYSD-------LDNHSFESLHF----------GRKSIDTITPSE--FSQVSHDSDKSSSSQAMDDAEAEMRRLKLELKQTMEMYSTACKEALTAKQKTKELQHWKMEEEQRLEEARLAEEAALAIVEREKAKSRAAIEHAEAAQRIAELESQKRINAEMKAHKEAEEKNKVLNRLARSDIRYRKYKIEEIEAATNCFSESRKIGEGGYGPVYKCYLDHTQVAVKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLCRRGNTPPLSWQHRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSIGIMFLQILTAKPPMGLTHHIERAIEKGTFAEMLDPAVPDWPVEEALSFAKIALKCAELRRKDRPDLGKVVMPELERLRALAEENM---MADGSIHSPNHSQVSMCQDELSYPTTAQSGYDSS 778
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: F6H2V9 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 3 GHAERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSLPTLRFNSSPDVSPSSEANVLN-NRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPL--SSKLVRRPPME--KLSHSLPGESNLRPGDSNLRPGDSSFRSPFTR--KGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTFDSFETN-----STPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQASY 788
G+ E+ + L+AVAID+DKGSQ A+KWA+D IL++GQTV+L+HVKLK + S S P++ F+ S S + A + + +++FLPFR FCTRKDI C+DV++EDTDVV+ L +YV+ SA+E+LV+GA +KG FL RFK DIPG ++KG PDFCTVY+ISKGKISSTR+ASR APA SPLRNQI +Q S R +P +P P SS+ V +PP+E + SH +SPFTR +GPNGR Y E+S P++DISF SSGRPST+RM+ F +++N S PRLSN S D+D +T+ +S GR S++ +P E S E+D S A+D++AEMRRLKLELKQTMDMYSTACKEALSAKQKA ELQRWK EEEQRLEEAR+AEE ALA+AEREKA+S+AAIEHAEAA+R+AE EAQKRINAE KA++ EE+ K S+AQ++ RYRKYTI+EIE ATEFF++SRKIGEGGYGPV+KC LDHT VA+KVLRPDAAQGRSQFQQEVE+LSCIRHPNMVLLLGACPEYGCLVYEYM+NGSLEDRL ++G+S PLSWQ RFRIAAEI TGLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP +V D+VTQYRMTSTAGTFCYIDPEYQQTGMLG+KSDVYSLGIM LQ+ITAK PMG+TH+VE AIEK TF E+LDP VPDWP+EEALSFAKI+LQCAELRRKDRPDLG +LPEL RLRE +E+++ M+ G+ Y SQ S+
Sbjct: 6 GNTEKKEGGNPLVAVAIDKDKGSQSALKWAIDNILNRGQTVVLIHVKLKQ----SHSHSYPSISFSPSFINSSLTVAKLDHYTKELFLPFRCFCTRKDIQCKDVILEDTDVVKALIEYVSHSAIEVLVVGAPAKGGFL-RFKAIDIPGSITKGAPDFCTVYVISKGKISSTRSASRAAPAISPLRNQIMNQG-SIRPDPSDLPIPHAPSSRAVEKPPVEPPRSSHD----------------DFEHIKSPFTRGARGPNGRSYGEISLPDSDISFVSSGRPSTDRMFPPF--YDSNIDIIRSNPRLSNSS---DMDN--QTF------------ESSQMGRKSMEIGSPPEFLAISQENDRTSWSSQSAEDVEAEMRRLKLELKQTMDMYSTACKEALSAKQKARELQRWKLEEEQRLEEARLAEEAALAIAEREKAKSRAAIEHAEAAQRIAELEAQKRINAEMKAIKEAEEKKKVLDSLAQTDVRYRKYTIEEIESATEFFSESRKIGEGGYGPVYKCNLDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMANGSLEDRLFRRGDSPPLSWQLRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGIKSDVYSLGIMLLQIITAKPPMGLTHLVERAIEKDTFEEMLDPVVPDWPLEEALSFAKIALQCAELRRKDRPDLGKAVLPELNRLRELSEEHLDPTMMGGS----QYHSTNHSQVSF 761
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A1U8ESR1 ((U-box domain-containing protein 52-like isoform X2 {ECO:0000313|RefSeq:XP_016549421.1}))
Query: 4 HAERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSLPTLRFNSSPDVSPSSEANV-LNNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISK-GKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPLSSKLVRRPPMEKLSHSLPGESN--LRPGDSNLRPGDSSFRSPFT-RKGPNGRPYELSNPNNDISFTSSGRPSTERMYSTF-DSFETN--STPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQ--ASYPQSGFSSTGNS 799
ER +KL+AVAID+DKGSQ A+KW VD +L++GQTVLL+HVKLK A+++ S + N D S + RD+FLPFRVFCTRKDI C DVV++DTDV + + +YV + VEILV+GA +KG L RFK KDIPGG+ KG PDFCTV+IISK GKISSTRAASR AP PLR+Q+ + + S PS +S+ +S PG + P S ++ S +SPFT RKGPNG+PYELS P+ DIS+ SSGRPS + +Y++ DS+E+ + PRLS FS+F +S D T+ GR SVDT TP E S AS+E D P DDL+AEMRRLK EL +TM+MYSTACKEAL+AKQKAMELQRWK EE++RLEEAR+AEE ALA+AEREKARS+AAIEHAEA++RLAE EAQKRI+AE KA++ EE+NK ++ S+ RYRKYTI+EIE AT++FA+SRKIGEGGYGPV+KC LDHT VAVKVLRPDA GR QFQQE+E+LSCIRHPNMVLLLGACPEYGCLVYE+MSNGSL+DRL +G + PLSWQ RFRIAAEIATG+LFLHQ+KPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVP +V D VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGI++LQ++TA+SPMG+TH VE AIEKGTF E+LDP++ DWP++EA+ A +SLQC+ELRRKDRPDLG V+LPELERLR AE+N +++Y SP +SQ ++S+ SYP + SS +S
Sbjct: 9 QGERMPLPNKLVAVAIDKDKGSQIALKWTVDHLLARGQTVLLIHVKLKQSANASGQSS----KSNQLSDEGGSGNIEMDQQTRDLFLPFRVFCTRKDIQCYDVVLDDTDVAKAVVEYVNRTGVEILVVGATTKGGLL-RFKAKDIPGGMLKGAPDFCTVHIISKSGKISSTRAASRPAPFVHPLRHQLMQTASTKFSFESSTPSSATSR-----------NSFPGAAKPVFDPPPSTIQSDTMSIKSPFTHRKGPNGKPYELSLPDTDISYVSSGRPSVDNIYASLSDSYESGAPTPPRLSGFSDFDT----------QSFDSTQF-------GRRSVDTLTPPELSIASIEGDRTSFSQGPGDDLEAEMRRLKQELSKTMEMYSTACKEALTAKQKAMELQRWKMEEKRRLEEARLAEEAALAIAEREKARSRAAIEHAEASQRLAELEAQKRISAEMKAIKEAEEKNKILNKLSNSDSRYRKYTIEEIESATDYFAQSRKIGEGGYGPVYKCYLDHTPVAVKVLRPDATHGRQQFQQEIEVLSCIRHPNMVLLLGACPEYGCLVYEFMSNGSLDDRLFHRGKTPPLSWQQRFRIAAEIATGVLFLHQSKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPSVADTVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIIFLQILTARSPMGLTHYVERAIEKGTFHEMLDPAIRDWPLDEAMCLANLSLQCSELRRKDRPDLGKVVLPELERLRTLAEENTCPSLMYNLNISPNHSQVSLSRDNLSYPNTMPSSYESS 783
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A1U8F193 ((U-box domain-containing protein 52-like isoform X1 {ECO:0000313|RefSeq:XP_016549415.1}))
Query: 4 HAERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLK--SPASSNSSPSL----PTLRFNSSPDVSPSSEANVLNN-------------RDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISK-GKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPLSSKLVRRPPMEKLSHSLPGESN--LRPGDSNLRPGDSSFRSPFT-RKGPNGRPYELSNPNNDISFTSSGRPSTERMYSTF-DSFETN--STPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQ--ASYPQSGFSSTGNS 799
ER +KL+AVAID+DKGSQ A+KW VD +L++GQTVLL+HVKLK + AS SS L P SS D +E+N L++ RD+FLPFRVFCTRKDI C DVV++DTDV + + +YV + VEILV+GA +KG L RFK KDIPGG+ KG PDFCTV+IISK GKISSTRAASR AP PLR+Q+ + + S PS +S+ +S PG + P S ++ S +SPFT RKGPNG+PYELS P+ DIS+ SSGRPS + +Y++ DS+E+ + PRLS FS+F +S D T+ GR SVDT TP E S AS+E D P DDL+AEMRRLK EL +TM+MYSTACKEAL+AKQKAMELQRWK EE++RLEEAR+AEE ALA+AEREKARS+AAIEHAEA++RLAE EAQKRI+AE KA++ EE+NK ++ S+ RYRKYTI+EIE AT++FA+SRKIGEGGYGPV+KC LDHT VAVKVLRPDA GR QFQQE+E+LSCIRHPNMVLLLGACPEYGCLVYE+MSNGSL+DRL +G + PLSWQ RFRIAAEIATG+LFLHQ+KPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVP +V D VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGI++LQ++TA+SPMG+TH VE AIEKGTF E+LDP++ DWP++EA+ A +SLQC+ELRRKDRPDLG V+LPELERLR AE+N +++Y SP +SQ ++S+ SYP + SS +S
Sbjct: 9 QGERMPLPNKLVAVAIDKDKGSQIALKWTVDHLLARGQTVLLIHVKLKQSANASGQSSTCLDLDYPMEYLLSSTD----TESNQLSDEGGSGNIEMDQQTRDLFLPFRVFCTRKDIQCYDVVLDDTDVAKAVVEYVNRTGVEILVVGATTKGGLL-RFKAKDIPGGMLKGAPDFCTVHIISKSGKISSTRAASRPAPFVHPLRHQLMQTASTKFSFESSTPSSATSR-----------NSFPGAAKPVFDPPPSTIQSDTMSIKSPFTHRKGPNGKPYELSLPDTDISYVSSGRPSVDNIYASLSDSYESGAPTPPRLSGFSDFDT----------QSFDSTQF-------GRRSVDTLTPPELSIASIEGDRTSFSQGPGDDLEAEMRRLKQELSKTMEMYSTACKEALTAKQKAMELQRWKMEEKRRLEEARLAEEAALAIAEREKARSRAAIEHAEASQRLAELEAQKRISAEMKAIKEAEEKNKILNKLSNSDSRYRKYTIEEIESATDYFAQSRKIGEGGYGPVYKCYLDHTPVAVKVLRPDATHGRQQFQQEIEVLSCIRHPNMVLLLGACPEYGCLVYEFMSNGSLDDRLFHRGKTPPLSWQQRFRIAAEIATGVLFLHQSKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPSVADTVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIIFLQILTARSPMGLTHYVERAIEKGTFHEMLDPAIRDWPLDEAMCLANLSLQCSELRRKDRPDLGKVVLPELERLRTLAEENTCPSLMYNLNISPNHSQVSLSRDNLSYPNTMPSSYESS 801
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A068VBY8 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
Query: 12 DKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSPSLPTLRFNSSPDVSPSSEANVLNNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQIKSQSPSSRSEPELVPSPL--SSKLVR----RPPMEKLSHSLPGESNLRPGDSNLRPGDSSFRSPFT-RKGPNGRPYELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTP-RLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDINGSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGTGPSPTYSQATMSQAS 787
+KLIAVAID+D+GSQ A+KW VD +L +GQT+LLVHVK K SS SS S+ NSS + + +D+FLPFRVFCTRKDIHCQDV++EDTDVVR L +YV+ V++LVLGA +KG RFK +DIP V KGVPDFC+VYIISKGKI++TR+AS AP + PLR Q+ Q+ SSRS P VP P+ SS+ + R E S ++ +S NL G RSPFT RKGPNG+PYELS+P+ DISF SSGRPS + + +F DS ++ TP R+S FS + +GRRSVD T ++S D + S++ DD++ EMRRLKLELKQTM+MYSTACKEALSAKQKA + + LEEAR+AEETALALAE+EK +S EH EAA+R+AE E QKRINAE K L+ EE NK + QS RYR YTIDEIE AT++F KS KIGEGGYGPV+KC LDHT VAVKVLRPDAA GRSQFQQEVE+LSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRL ++GN+ L WQHRFRIAAEI GLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP +V + VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD+YSLGI++LQ++T K PMG+TH VE AIEKGTF+E++DP++PDWPIEEAL+ AKI+L+C+ELRRKDRPDLG V++PELERLR F E+ M+ ++ Y +G SP SQ ++S S
Sbjct: 5 NKLIAVAIDKDRGSQNALKWTVDNLLVRGQTILLVHVKAKLHGSSFSSSSVALPITNSSDEYGMYGRDYDVETKDIFLPFRVFCTRKDIHCQDVILEDTDVVRALLEYVSRIGVDVLVLGAAAKGYLFRRFKARDIPASVLKGVPDFCSVYIISKGKIAATRSASCSAPVNHPLRQQMLDQA-SSRSSPLDVPLPIIHSSRCSQLSSTRQASEPSSRNIKTDSTTMNQSGNLL-GLIENRSPFTHRKGPNGKPYELSSPDTDISFVSSGRPSIDNTFPSFADSIDSGPTPPRISGFSEMENRSFESLHFGRRSVD-------------------IITSPEFSSAFGDSDASMAQATDDMEVEMRRLKLELKQTMEMYSTACKEALSAKQKAWKTHGKILKPIILLEEARLAEETALALAEKEKTKSGVVAEHVEAAQRIAELETQKRINAEMKTLKEMEENNKLFNRLVQSNIRYRVYTIDEIEIATQYFDKSLKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAHGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLFRRGNTPALPWQHRFRIAAEITIGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIIFLQILTGKPPMGLTHQVERAIEKGTFTEMIDPAIPDWPIEEALNLAKIALKCSELRRKDRPDLGKVVMPELERLRAFGEEKMSFSLTYSSGQSPMQSQVSLSTVS 770
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A5B6Z458 ((Putative U-box domain-containing protein 52 {ECO:0000313|EMBL:MPA38256.1}) (1.8.1.8 {ECO:0000313|EMBL:MPA38256.1}))
Query: 5 AERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSP-SLPTLRFNSSPDVSPSSEANVL-----NNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQ------IKSQSPSSRSEPEL----VPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSFRSPFT-RKGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDIN-GSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGT-GPSPTYSQATMSQ--ASYPQSG 792
ER ++L+AVAIDRDKGSQ A+KWAVD +L KGQTV++VHVK+K S +S + P R N DV+ VL +++FLPFR FCTRKDI QDVV+EDTDVV+ LT+YV+ SA+EILVLGA SKG L RFK DIPG VS+GVPDFCTVY+ISKGKISSTR+A+R P SP RN IK + S P + VP P S + +P E L H++ + + +SPFT RKGPNGR Y E S P+ DISF SSGRPST+ M+ +F DSFE + RLS+ S + + + GR S+DT +P + ++SV D N S S +D++ EMRRLK+ELKQTMD+YSTACKEAL+AKQKA ELQ WK EE+QRLEEAR+AEE A+A+AE+EKA+SKAAIE AEAA+R+AE EAQKRINAE KAL+ E++NK ++A+S+ RYR+YTI+EIE ATEFF++SRKIGEGGYGPV+KC LDHT VAVKVLRPDAAQGRSQFQQE+EILSCIRHPNMVLL+GACPEYGCLVYEYMSNGSLEDRL ++G++ PLSWQHRFRIAAEI TGLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP ++ D VTQYRMTSTAGTFCYIDPEYQQTGMLG+KSD+YSLGIM+LQ+ITAK PMG+TH VE AIEKGTF+E+LDP+VPDWP+EEALSFAK++L+C ELRRKDRPD+ V++PEL+RLR+ AE+NM+ +M + GPSP +SQ +MSQ SYP G
Sbjct: 9 GERREIANRLVAVAIDRDKGSQAALKWAVDNLLMKGQTVVMVHVKVKPSPSHSSPSLATPLPRVNQVFDVN-DENGQVLAESDPQTKELFLPFRCFCTRKDIQSQDVVLEDTDVVKALTEYVSRSAIEILVLGAASKGGLLRRFKMTDIPGSVSRGVPDFCTVYVISKGKISSTRSATRPPPPISPFRNHLLHPDSIKPDISETPSPPAIHSKGVPRPYSESGIPKPQSELLQHNMYNDMDF-------------IKSPFTHRKGPNGRSYGEFSQPDTDISFVSSGRPSTDHMFPSFYDSFEMSQIQRLSSCS-----------------EADNHSFESLHFGRYSLDTISPPD--FSSVSHDSNRSSTSQTMEDVEVEMRRLKMELKQTMDLYSTACKEALTAKQKATELQHWKIEEQQRLEEARLAEEAAMAIAEKEKAKSKAAIEAAEAAQRIAELEAQKRINAEMKALKEAEDKNKVLKALAKSDVRYRRYTIEEIEAATEFFSESRKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEIEILSCIRHPNMVLLVGACPEYGCLVYEYMSNGSLEDRLFRRGSTTPLSWQHRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSIADTVTQYRMTSTAGTFCYIDPEYQQTGMLGIKSDIYSLGIMFLQIITAKPPMGLTHHVERAIEKGTFAEMLDPAVPDWPVEEALSFAKMALKCGELRRKDRPDVSKVVMPELDRLRKLAEENMSHSMFSSSAGPSPNHSQVSMSQDELSYPTFG 788
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A5B6Z2J0 ((Putative U-box domain-containing protein 52 {ECO:0000313|EMBL:MPA38255.1}) (1.8.1.8 {ECO:0000313|EMBL:MPA38255.1}))
Query: 5 AERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSP-SLPTLRFNSSPDVSPSSEANVL-----NNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQ------IKSQSPSSRSEPEL----VPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSFRSPFT-RKGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDIN-GSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGT-GPSPTYSQATMSQ--ASYPQSGFSSTGNSAA 801
ER ++L+AVAIDRDKGSQ A+KWAVD +L KGQTV++VHVK+K S +S + P R N DV+ VL +++FLPFR FCTRKDI QDVV+EDTDVV+ LT+YV+ SA+EILVLGA SKG L RFK DIPG VS+GVPDFCTVY+ISKGKISSTR+A+R P SP RN IK + S P + VP P S + +P E L H++ + + +SPFT RKGPNGR Y E S P+ DISF SSGRPST+ M+ +F DSFE + RLS+ S + + + GR S+DT +P + ++SV D N S S +D++ EMRRLK+ELKQTMD+YSTACKEAL+AKQKA ELQ WK EE+QRLEEAR+AEE A+A+AE+EKA+SKAAIE AEAA+R+AE EAQKRINAE KAL+ E++NK ++A+S+ RYR+YTI+EIE ATEFF++SRKIGEGGYGPV+KC LDHT VAVKVLRPDAAQGRSQFQQE+EILSCIRHPNMVLL+GACPEYGCLVYEYMSNGSLEDRL ++G++ PLSWQHRFRIAAEI TGLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP ++ D VTQYRMTSTAGTFCYIDPEYQQTGMLG+KSD+YSLGI++LQ+ITAK PMGITH +E AIEKGTF E+LDP+VPDWP+EEALSFAK++L+CAELRRKDRPDLG V++PELERLR AE++M+ M + GPSP SQ +MSQ SYP S N ++
Sbjct: 9 GERREIANRLVAVAIDRDKGSQAALKWAVDNLLMKGQTVVMVHVKVKPSPSHSSPSLATPLPRVNQVFDVN-DENGQVLAESDPQTKELFLPFRCFCTRKDIQSQDVVLEDTDVVKALTEYVSRSAIEILVLGAASKGGLLRRFKMTDIPGSVSRGVPDFCTVYVISKGKISSTRSATRPPPPISPFRNHLLHPDSIKPDISETPSPPAIHSKGVPRPYSESGIPKPQSELLQHNMYNDMDF-------------IKSPFTHRKGPNGRSYGEFSQPDTDISFVSSGRPSTDHMFPSFYDSFEMSQIQRLSSCS-----------------EADNHSFESLHFGRYSLDTISPPD--FSSVSHDSNRSSTSQTMEDVEVEMRRLKMELKQTMDLYSTACKEALTAKQKATELQHWKIEEQQRLEEARLAEEAAMAIAEKEKAKSKAAIEAAEAAQRIAELEAQKRINAEMKALKEAEDKNKVLKALAKSDVRYRRYTIEEIEAATEFFSESRKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEIEILSCIRHPNMVLLVGACPEYGCLVYEYMSNGSLEDRLFRRGSTTPLSWQHRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSIADTVTQYRMTSTAGTFCYIDPEYQQTGMLGIKSDIYSLGIIFLQIITAKPPMGITHHIERAIEKGTFVEMLDPAVPDWPVEEALSFAKLALKCAELRRKDRPDLGKVVMPELERLRTLAEESMSHTMFSNSAGPSPNQSQVSMSQDELSYPMLAQSDYDNRSS 797
BLAST of DCAR_000676 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A5B6Z2H1 ((Putative U-box domain-containing protein 52 {ECO:0000313|EMBL:MPA38254.1}) (1.8.1.8 {ECO:0000313|EMBL:MPA38254.1}))
Query: 5 AERNAAKDKLIAVAIDRDKGSQGAMKWAVDAILSKGQTVLLVHVKLKSPASSNSSP-SLPTLRFNSSPDVSPSSEANVL-----NNRDVFLPFRVFCTRKDIHCQDVVVEDTDVVRGLTQYVTDSAVEILVLGAGSKGSFLSRFKGKDIPGGVSKGVPDFCTVYIISKGKISSTRAASRVAPASSPLRNQ------IKSQSPSSRSEPEL----VPSPLSSKLVRRPPMEKLSHSLPGESNLRPGDSNLRPGDSSFRSPFT-RKGPNGRPY-ELSNPNNDISFTSSGRPSTERMYSTF-DSFETNSTPRLSNFSNFSDLDQGLETYGRRSVDGTRSNGRTSVNGRISVDTFTPTERSYASVESDIN-GSLSPPADDLDAEMRRLKLELKQTMDMYSTACKEALSAKQKAMELQRWKKEEEQRLEEARIAEETALALAEREKARSKAAIEHAEAARRLAESEAQKRINAEKKALQH-EERNKTPTSMAQSE-RYRKYTIDEIEQATEFFAKSRKIGEGGYGPVFKCRLDHTAVAVKVLRPDAAQGRSQFQQEVEILSCIRHPNMVLLLGACPEYGCLVYEYMSNGSLEDRLLQKGNSKPLSWQHRFRIAAEIATGLLFLHQTKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPSTVTDDVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDVYSLGIMYLQLITAKSPMGITHIVENAIEKGTFSEILDPSVPDWPIEEALSFAKISLQCAELRRKDRPDLGSVILPELERLREFAEDNMTDAMLYGT-GPSPTYSQATMSQAS 787
ER ++L+AVAIDRDKGSQ A+KWAVD +L KGQTV++VHVK+K S +S + P R N DV+ VL +++FLPFR FCTRKDI QDVV+EDTDVV+ LT+YV+ SA+EILVLGA SKG L RFK DIPG VS+GVPDFCTVY+ISKGKISSTR+A+R P SP RN IK + S P + VP P S + +P E L H++ + + +SPFT RKGPNGR Y E S P+ DISF SSGRPST+ M+ +F DSFE + RLS+ S + + + GR S+DT +P + ++SV D N S S +D++ EMRRLK+ELKQTMD+YSTACKEAL+AKQKA ELQ WK EE+QRLEEAR+AEE A+A+AE+EKA+SKAAIE AEAA+R+AE EAQKRINAE KAL+ E++NK ++A+S+ RYR+YTI+EIE ATEFF++SRKIGEGGYGPV+KC LDHT VAVKVLRPDAAQGRSQFQQE+EILSCIRHPNMVLL+GACPEYGCLVYEYMSNGSLEDRL ++G++ PLSWQHRFRIAAEI TGLLFLHQTKPEPLVHRDLKP NILLDRN+VSKISDVGLARLVP ++ D VTQYRMTSTAGTFCYIDPEYQQTGMLG+KSD+YSLGI++LQ+ITAK PMGITH +E AIEKGTF E+LDP+VPDWP+EEALSFAK++L+CAELRRKDRPDLG V++PELERLR AE++M+ M + GPSP SQ +MSQ S
Sbjct: 9 GERREIANRLVAVAIDRDKGSQAALKWAVDNLLMKGQTVVMVHVKVKPSPSHSSPSLATPLPRVNQVFDVN-DENGQVLAESDPQTKELFLPFRCFCTRKDIQSQDVVLEDTDVVKALTEYVSRSAIEILVLGAASKGGLLRRFKMTDIPGSVSRGVPDFCTVYVISKGKISSTRSATRPPPPISPFRNHLLHPDSIKPDISETPSPPAIHSKGVPRPYSESGIPKPQSELLQHNMYNDMDF-------------IKSPFTHRKGPNGRSYGEFSQPDTDISFVSSGRPSTDHMFPSFYDSFEMSQIQRLSSCS-----------------EADNHSFESLHFGRYSLDTISPPD--FSSVSHDSNRSSTSQTMEDVEVEMRRLKMELKQTMDLYSTACKEALTAKQKATELQHWKIEEQQRLEEARLAEEAAMAIAEKEKAKSKAAIEAAEAAQRIAELEAQKRINAEMKALKEAEDKNKVLKALAKSDVRYRRYTIEEIEAATEFFSESRKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEIEILSCIRHPNMVLLVGACPEYGCLVYEYMSNGSLEDRLFRRGSTTPLSWQHRFRIAAEIGTGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSIADTVTQYRMTSTAGTFCYIDPEYQQTGMLGIKSDIYSLGIIFLQIITAKPPMGITHHIERAIEKGTFVEMLDPAVPDWPVEEALSFAKLALKCAELRRKDRPDLGKVVMPELERLRTLAEESMSHTMFSNSAGPSPNQSQVSMSQVS 781
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.
There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.
LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.
Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.
RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.
The NCBI Sequence report lists the correspondences between the various naming methods
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.
