Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLP------LQSPLSDDL-SSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWLGSK-SSRSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKR-NFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNL-TPGSSQYSNYIGKGNTVLQTPKLDAKHYGSFSDGPPYVKDIVAPVRTGEY--SSVDDITKANSQSFCSTP-SLAQSIVCTS-SPESILRNSAMKYEHTPSIIRKSRPKEGGSC--------SPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVL 629
+RSSQAGWTEEEDN L++VVKK+NGRNWKKIA +PGRTDVQCLHRWQKVLNPEL KGPW KEEDDRII+LV+KYG +KWS IAK+LPGRIGKQCRERWHNHLDPAIKKEAW++ EE++L++YH+IYGNKWAEIAKFLPGRTDNAIKNHWNCS KKKLD LNLP +Q P S D SE SV+ + + +A ++ I + CST+L LGNAY+ + L SK S R + + V++ +S P +R F ++ +A L+S +G +P G + AS + D + FN+ I S P +DV P T K E+ +R R+ G + L NS L+L T G S+ S + K N V QTP D K+YG PP +KD + P E ++ +D+ SQ CS P +LA S+ +S SPES+LRNSA Y++TPSIIRK ++ GS + H IS T E+ + N RS S+ + +ERRL+ FD EWD AVRC TPVSA + L
Sbjct: 109 TRRSSQAGWTEEEDNLLSEVVKKYNGRNWKKIAACIPGRTDVQCLHRWQKVLNPELVKGPWTKEEDDRIIELVEKYGCKKWSFIAKFLPGRIGKQCRERWHNHLDPAIKKEAWTKEEESVLTHYHRIYGNKWAEIAKFLPGRTDNAIKNHWNCSVKKKLD---LNLPSTSMLDMQGPSSPDFYGSETKPPSVEAKVARYDLREA--SFDIRSSKVHAIETCSTELVLGNAYSRGTCLESKLSIRGTRRSSEAGVDKLIS-----------------PLRRIQFRGTDTMACGLTSEPY--------------RGNCYPDRHGHLKAS-----SSDDS-FNASGLVISSFSP--------LDVVLP-----------------ITSIKAFESPKRHRNCGLGGMKLESGSSSDNSFLSLSTGGFSEDSGQVCKKNRVHQTPNPDDKNYGFLCYKPPQLKDFLIPEENDEGCPNADNDLKHGKSQLCCSPPPNLALSMSISSISPESMLRNSARSYKNTPSIIRKRTSRDSGSSNNYSDRNKTSEHMISRTSEAESMNI---------------RSETSIVDRSVERRLQGEFDNEWDTTAVRCRTPVSAATSPSL 640
Query: 2 VKKENEGVI-VKKEIDFVSS-SSISDSSCDTGYKRN----------KVAKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLP------LQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSD--MCSTDLTLGNAYTCESWLGSKSSRSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRS--DGSNIINLRRECVQGNSLLNL-TPGSSQYSNYIGKGNTVLQTPKL-DAKHYGSFSDGPPYVKDIVAPVR-TGEYSSVDD-ITKANSQSFCST-PSLAQSI-VCTSSPESILRNSAMKYEHTPSIIRKSRPKEGGSCSPVHK----ISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVL 629
VK+E E ++ VKKE+ F ++ S+SDSSCDT R+ V +RSSQAGWTEEEDN L++VV+K+NGRNWKKIAE +PGRTDVQCLHRWQKVLNPEL KGPW KEED+ II LV+KYG +KWS IAK L GRIGKQCRERWHNHLDPAIKKEAW+ EEAIL+YYHQIYGNKWAE+A+FLPGRTDNAIKNHWNCS KKK DLNLP +Q S D S E+ + + +S + KV D CST+L LGNA + L + P SR+QF G + G +T E G + P G+ PL + + FNS + S++ + P FT K E+ +R RS G + NL + NS L+L T G ++ + + K N + P D K+YG S P +KD V P + G + SVD ++ NS CST P L S+ V +S P S+LRNSAM Y++TPSIIRK ++ S + + M +R + +++ D S HRS SV + LERRLE FD+EWD AVRC TPVS + L
Sbjct: 4 VKEEEECIVDVKKEVGFAAAWFSVSDSSCDTYTPRSPSARSHRRITGVTRRSSQAGWTEEEDNLLSEVVEKYNGRNWKKIAECIPGRTDVQCLHRWQKVLNPELVKGPWTKEEDEHIIYLVEKYGCKKWSVIAKCLHGRIGKQCRERWHNHLDPAIKKEAWTIEEEAILTYYHQIYGNKWAELARFLPGRTDNAIKNHWNCSVKKK---FDLNLPPSPVLDMQGTASPDFCSS---ETKSVGTDDILARHGLSDESLDCRRAKVHDHETCSTELVLGNAKLIVNPLELNPYSAGVEAGGNKQKSPSSRIQFSGTYTGARG-------------------------LTSEPCQGDS---PLGIHDPLKASS--------HDDFFHAFNSTKYAF-----------NSLNADLP-----------------FTSEKTFESSKRHRSCGRGLGVANLESGSLSENSCLSLSTFGINEPYDQVYKKNRIHAIPHHSDDKYYGYLSYKSPQLKDFVTPKKNVGGWLSVDSYLSHDNSHVCCSTPPDLELSMSVNSSCPGSMLRNSAMSYKNTPSIIRKRSSRKAVSSNYSDRNYTSARMISCTREVDFMNVKQDGAVCLS--HRSEASVVDRSLERRLECEFDKEWDSAAVRCCTPVSTTGASGL 591
BLAST of DCAR_004664 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A6A4L078 ((Uncharacterized protein {ECO:0000313|EMBL:KAE9450902.1}) (Fragment))
Query: 2 VKKENEGVI-VKKEIDFVSS-SSISDSSCDT---------GYKR-NKVAKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLPLQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGP---VRKV----SDMCSTDLTLGNAYTCESWLGSKSSRSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEA-SGGKTNLAPQGVLHPLTSGGMFASCKRPRNG--DVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQTP-KLDAKHYGSFSDGPPYVKDIVAPVR-TGEYSSVDD-ITKANSQSFCST-PSLAQSI-VCTSSPESILRNSAMKYEHTPSIIRKSRPKEGGSCSPVHK----ISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSA 623
VK+E E ++ VKKE+ F ++ SS+SDSSCDT G++R V +RSSQAGWTEEEDN L++VV+K+NGRNWKKIAE +PGRTDVQCLHRWQKVLNPEL KGPW KEED+ II LV+KYG +KWS IAK+L GRIGKQCRERWHNHLDPAIKKEAW+ EEAIL+YYHQIYGNKWAE+A+FLPGRTDNAIKNHWNCS KKK DLNLP L D+ A+ + +G ++ + ++ R+ S+ CST+L LGNA + L + P SR+QF G + G P + N + +SS + A K P P TS F S KR R+ + + N E S LS+ +S S I N PY V+ K N V P D K+YG S PP +KD V P G + SVD ++ NS CST P L S+ V +S P S+LRNSAM Y++TPSIIRK ++ S + + M +R + +++ D S HRS SV + LERRLE FD+EWD AVRC TPVS
Sbjct: 4 VKEEEECIVDVKKEVGFAAAWSSVSDSSCDTYTPRSLSARGHRRITGVTRRSSQAGWTEEEDNLLSEVVEKYNGRNWKKIAECIPGRTDVQCLHRWQKVLNPELVKGPWTKEEDEHIIYLVEKYGCKKWSVIAKFLHGRIGKQCRERWHNHLDPAIKKEAWTIEEEAILNYYHQIYGNKWAELARFLPGRTDNAIKNHWNCSVKKK---FDLNLPPSPVL--DMQGTASPDFCSSETKSVGTDDKLARHGLSEESLDCRRAKVHDSETCSTELVLGNAKLIVNPLELNPYSAGVEAGGNKQISPSSRIQFSGTHTGARGLTSEPCQGNRSPLGIHDPLKASSHDVFFHAFDSTKYTFNPLNADLPFTSEKTFESSKRHRSCGRGLGVANLESGS-LSENSCLSLSTFGI-------------NEPYDQVY-------------------------------------------------KKNRVHAIPYHSDDKYYGYLSYKPPQLKDFVTPKENVGGWLSVDSYLSHDNSHVCCSTPPDLELSMSVNSSCPGSMLRNSAMSYKNTPSIIRKRSSRKAVSSNYSDRNYTSARMISCAREVDFMNVKQDGAVCPS--HRSEASVLDRSLERRLECEFDKEWDSAAVRCCTPVST 586
Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLP------LQSPLSDDL-SSEANLESVKIPAVQLGVGKAMSTYQI-AGPVRKVSDMCSTDLTLGNAYTCESWLGSKSSRSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKR-NFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNL-TPGSSQYSNYIGKGNTVLQTPKLDAKHYGSFSDGPPYVKDIVAPVRTGEY--SSVDDITKANSQSFCSTP-SLAQSIVCTS-SPESILRNSAMKYEHTPSIIRKSRPKEGGSC--------SPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVL 629
+RSSQAGWTEEEDN L+ +VKK+NGRNWKKIA +PGRTDVQCLHRWQKVLNPEL KGPW KEEDDRII+LV+KYG +KWS IAK+LPGRIGKQCRERWHNHLDPAIKKEAW++ EE++L++YH+IYGNKWAEIAKFLPGRTDNAIKNHWNCS KKKLD LNLP +Q P S D SE SV+ + + +A ++ I +G V + + CST+L LGNA Y + LS R +S K+ P +R F ++ + L+S +G +P G + AS + D + FN+ I S P +DV P T K E+ +R R+ G + L NS L+L T G S+ S + K N V QTP D K+YG PP +KD + P E ++ +D+ SQ CS P +LA S+ S SPES+LRNSA Y++TPSIIRK ++ GS + H IS T E+ + N RS S+ + +ERRL+ FD EWD AVRC TPVSA + L
Sbjct: 111 TRRSSQAGWTEEEDNLLSKIVKKYNGRNWKKIAACIPGRTDVQCLHRWQKVLNPELVKGPWTKEEDDRIIELVEKYGCKKWSCIAKFLPGRIGKQCRERWHNHLDPAIKKEAWTKEEESVLTHYHRIYGNKWAEIAKFLPGRTDNAIKNHWNCSVKKKLD---LNLPSTSMLDMQGPSSPDFYGSETKPPSVEAKVARYDLREA--SFDIRSGKVHAI-ETCSTELVLGNA----------------YSHGTCLESKLSIRGTRRSSEAGVDKLISPLRRIQFRGTDAMTRGLTSEPY--------------RGNCYPDRHGHLKAS-----SSDDS-FNASGLVISSFNP--------LDVVLP-----------------ITSIKAFESPKRHRNCGLGGMKLESGSSSDNSFLSLSTGGFSEDSGQVCKKNRVHQTPNPDDKNYGFLCYKPPQLKDFLIPEENDEGCPNADNDLKHGKSQLCCSPPPNLALSMSINSISPESMLRNSARSYKNTPSIIRKRTSRDSGSSNNYPDRNKTSEHMISRTSEAESMNI---------------RSETSIVDRSVERRLQGEFDNEWDTTAVRCRTPVSAATSPSL 642
Query: 2 VKKENEGVI-VKKEIDFVSSSSISDSSCDTGYKRN----------------KVAKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLPLQSPLSDDLSSEANLESVKIPAV--QLGVGKAMST---YQIAGPVRKVSDMCSTDLTLGNAYTCESWLGSKSSRSQKYPEQEA----VNQPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNL-TPGSSQYSNYIGKGNTVLQTPKLDAKHYGSFSDGPPYVKDIVAPVRTG-EYSSVDD-ITKANSQ-SFCSTPSLAQSI-VCTSSPESILRNSAMKYEHTPSIIRKSRPKEGGS-------CSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVL 629
VKKE E V+ + KE+ + S +SDSS DT +R+ +RSSQAGWTEEEDN L++ VKK+NGRNWKKIAE L GRTDVQCLHRWQK EDD II+LV+KYG +KWS IAK+LPGRIGKQCRERWHNHLDPA+KKEAW++ EEAIL+ YH+IYGN+WAE+A+FLPGRTDNAIKNHWNCS KKK D LNLP S S L++ + S + +V ++ V + T ++ V D CST+L LGNA E+ L S S EA + PL R+QF G + +S +T E S G + +G +S + + SCS S P +V T K E+ +R R +++NL + NS L L T G +++ ++ K + V +T LD KH+G PP +KD V P G SV++ + +ANSQ F + P LA SI +SSPES+LRNSAM Y++TPSIIRK +E GS C+P IS T E+ D ++ K HS HR +SVA K ERRLE FD+EWD RC TPVS + + L
Sbjct: 4 VKKEEEYVMDLTKEVGLSTFSPVSDSSGDTFTQRSPSSQAADWTLFFSLRKTTIRRSSQAGWTEEEDNLLSEYVKKYNGRNWKKIAECLSGRTDVQCLHRWQK--------------EDDCIIELVEKYGCKKWSVIAKFLPGRIGKQCRERWHNHLDPAVKKEAWTKEEEAILTCYHKIYGNRWAELARFLPGRTDNAIKNHWNCSVKKKWD---LNLP-PSLYSQFLAASPDFCSTETKSVCAEVTVDRHNLTEVSVDLSRGKEHVGDACSTELVLGNANLGENRLESNPSMLGACRFSEAGLNKLISPLRRIQFNGTAI--------------------------ASRLTNEPSQGNDHHDIRGTFQASSSDDLVVA--------------------------SCSTKS-------------SFTPLDVVLPVTTEKTFESPKRQRGRDLDVVNLESRSLLDNSFLCLSTFGVREHNGHVSKKSRVDETHHLDDKHFGCLCYKPPQLKDFVIPDENGGRCPSVENHVRRANSQVHFSTPPDLALSISFNSSSPESMLRNSAMSYKNTPSIIRKRASREAGSGNYSVRNCTPARMISCTSEA------DFVNVKQDAHSPFHRPKISVADKSRERRLECEFDKEWDSAVARCCTPVSTSASSGL 580
Query: 3 KKENEGVIVKKEIDFVSSSSISDSSCDTGYKR----------NKVAKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNLP----LQSPLSDD-LSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWLGSK-----SSRSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRN-----GDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQTPKLDAKHYGSFSDGPPYVKDIVAPVRTGEYSSVDDITKANSQSFCSTPSLAQSIVCTSSPESILRNSAMKYEHTPSIIRKSRPKEGG-------SCSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVS--APSEAVLGARLQ 634
K+E+ V + KE+ F + S+ SDSSCDT R KRSSQAGWTEEEDN L +VV+KFNGRNWKKIAE +PGRTDVQCLHRWQKVLNPEL KGPW KEEDD I++LV+KYG +KWS IAK LPGRIGKQCRERWHNHLDPAIKK+AW++ EE+IL+YYH+IYGNKWAE+A+ LPGRTDNAIKNHWNCS +KKL++ L LQS S D S E +++ + VG+++S Q G + +D CSTDL LGNA+ E SK +SRS + + +N PL+ +Q +I +G + P + N + + + +SSS + ASG P V+ P+TS M S KR D+ I +S S LS + +C G E Q+ KK K+ H TP P + V+ L G S+ N++ N+ FS P I SS + + + ++ S+ +TPS+ IRK +E G +C+P H S T + + DLM+ K L S +HRS SV+ K L RRLEYAFD EWDP VR PVS A S+A A +
Sbjct: 6 KEEDYTVDLTKEVGFTTCSA-SDSSCDTVIPRYPSVHCCRRTTGPIKRSSQAGWTEEEDNLLTEVVRKFNGRNWKKIAEIIPGRTDVQCLHRWQKVLNPELVKGPWTKEEDDHILKLVEKYGCKKWSVIAKSLPGRIGKQCRERWHNHLDPAIKKDAWTKEEESILTYYHKIYGNKWAELARLLPGRTDNAIKNHWNCSVRKKLESNLLRTSARDILQSSTSPDFFSCEVKAGCMEVTLGRQSVGESVSLDQKRG--LEYADPCSTDLVLGNAHLGEKCSESKPFMLGTSRSLEVGVNDLIN-PLTGIQVERMETITSGVIVEPCRGNANLAGIHKPLKASSSVDSSYASGSTKTHIPLEVVVPVTSKRMLESPKRSTGSGLCVNDLRIGSSSDNSFLS---FSACEFG----EENGQVGKK-----NKI--HGTP--------HPDDQNYGCLCYEPPQVKDLDLPMENAGCSRVDNHLRHANSSF-----------CFSTPPNLALSISV-----NGSSPESMLRNSAMSYKNTPSI---------------------------IRKRTYREAGNDIYSDCTCTPAHMSSCTPDKEVVDSTDLMNAKQGLLSPVHRSKTSVSDKSLGRRLEYAFDMEWDPATVRYCIPVSTTASSDANFAANMM 602
Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLD-NL--DLNLPLQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWLGSKSS-----RSQKYPEQEAVNQPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQT-PKLDAKHYGSFSDGPPYVKDIVAPVRTGEYSSVDDITKANSQSFCSTPSLAQSIVCTSSPESILRNSAMKYEHTPSIIRK--SRPKEG-------GSCSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVLGA 631
KRSSQAGWT EEDN L +VVK+F GRNWK IAE L GRTDVQCLHRWQKVLNPEL KGPW KEEDD II+LV+K+G +KWS IAK LPGRIGKQCRERWHNHLDP IK++AW+E E+ +L YHQIYGNKWAEIA+FLPGRTDNA+KNHWN + KK+LD NL L +QS S D SS +K Q + +S + AG V +D CSTDL +G A + + L +S +S ++ + +PL +QF + NG+ D + N + + + L+S + + + + + +HP T G M+ S KR + G V+ +D +++ S E Q +KK N Y + + + + N + +SL+ PG ++ + K N T L K G + PP VKD+ P+ S + I + NS CSTP +SSPES+LRNSAM Y TPSIIRK SR E G+ +P+H + + +D + L ++ SV+GK L RRLEYAFD EWD + RC TPVSA S +GA
Sbjct: 49 TKRSSQAGWTAEEDNLLTEVVKRFKGRNWKGIAECLNGRTDVQCLHRWQKVLNPELVKGPWTKEEDDHIIELVEKFGCKKWSIIAKSLPGRIGKQCRERWHNHLDPTIKRDAWTELEDLVLCRYHQIYGNKWAEIARFLPGRTDNAVKNHWNSTVKKRLDLNLLSRLGPDIQSEGSPDFSSHKKKLEIKKHLAQAHNAETLSLSKQAG-VDNAADACSTDLRIGCACSPRNDLHKGTSLFGACKSSVKNARDLI-KPLGGIQFGKAEILPNGETD---QSNLNLTKISDPLLTSLDFPLDPSRKTRWSSSQVKAVHPTTFGRMYESPKRSKYGTVSD---------TDHDFLTLSLAGF-TEVHSQGNKK--NKAYDIQSSLALKQPGFTEVHSKGNKKN-----KAYDTQSSLVLKQPGFTKIHSRGYKKNKAYDTQSSLAHKQPGFLNYEPPKVKDLTIPLTDENLSRDNIIGRQNSHLLCSTPLSLTVSANSSSPESVLRNSAMSYTKTPSIIRKKTSRFSEASGHYSCTGTTTPMHFL---------RSVPDREDTSNLKDRISGCKRSVSGKSLGRRLEYAFDMEWD--SSRCCTPVSAASPCAIGA 627
BLAST of DCAR_004664 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A2G2XKE7 ((Transcriptional activator Myb {ECO:0000313|EMBL:PHT57968.1}) (Fragment))
Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLD----LNLPLQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWL--GSKSSRSQKYPEQEAVN--QPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHH-------FTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQT-PKLDAKHYGSFSDGPPYVKDIVAPVRTGEYSSVDDITKANSQSFCSTPSLAQSIVCTSSPESILRNSAMKYEHTPSIIRK--SRPKEG-------GSCSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVLGA 631
KRSSQAGWT EEDN L +VVK+F GRNWK IAE L GRTDVQCLHRWQKVLNPEL KGPW KEEDD+II+LV+K+G +KWS IAK LPGRIGKQCRERWHNHLDP IK++AW+E+E+++L YHQIYGNKWAEIA+FLPGRTDNA+KNHWN S+ KK NL+ L +QS S D SS +K Q + + + G V +D CSTDL +G A + + L G+ + K E+ A + +PLS +QF + NG+ D + N + + + L+S + + + + + +HP T G M+ S KR + V +D +++ S E Q +KK N Y + FT +N + D + + L+R P ++ + K N T L K G + PP VKD+ P+ S + I + NS CSTP +SSPES+LRNSAM Y TPSIIRK SR E G+ +P+H + + +D + L ++ SV+GK L RRLEYAFD EWD + RC TPVSA S +GA
Sbjct: 50 TKRSSQAGWTAEEDNLLTEVVKRFKGRNWKGIAEFLNGRTDVQCLHRWQKVLNPELVKGPWTKEEDDQIIELVEKFGCKKWSIIAKSLPGRIGKQCRERWHNHLDPTIKRDAWTEQEDSVLCLYHQIYGNKWAEIARFLPGRTDNAVKNHWN-STVKKRSNLNLLSRLGPDIQSEGSPDFSSHKKKLEIKKHLAQAHNAETLCLSKQTG-VDNAADACSTDLRIGCACSPRNDLHKGTSLFGACKSSEKNARDLIKPLSGIQFGKAEILPNGETD---QSNLNLTKISDPLLTSLDFPLDPSRKTRWSSSQVKAVHPTTFGRMYESPKRSKYSTVND---------TDHDFLTLSLAGF-TEVHSQGNKK--NKAYDIQSSLALKQPGFTEVHSKDNKKNKAYDTQSSLVLKR------------PDFTKIHSRGYKKNKAYYTQSSLAHKQPGFLNYEPPKVKDLTIPLTDENLSRDNIIGRQNSHLLCSTPLSLTVSANSSSPESVLRNSAMSYTKTPSIIRKKTSRFSEASGHYSCTGTTTPMHFLRSVLD---------REDTSNLKDRISGCKRSVSGKSLGRRLEYAFDMEWD--SSRCCTPVSAASPCAIGA 628
BLAST of DCAR_004664 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A1U8GEZ9 ((uncharacterized protein LOC107865582 isoform X1 {ECO:0000313|RefSeq:XP_016567303.1}))
Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNL------PLQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWL--GSKSSRSQKYPEQEAVN--QPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQT-PKLDAKHYGSFSDGPPYVKDIVAPVRTGEYSSVDDITKANSQSFCSTPSLAQSIVCTSSPESILRNSAMKYEHTPSIIRK--SRPKEG-------GSCSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVLGA 631
KRSSQAGWT EEDN L +VVK+F GRNWK IAE L GRTDVQCLHRWQKVLNPEL KGPW KEEDD+II+LV+K+G +KWS IAK LPGRIGKQCRERWHNHLDP IK++AW+E E+++L YHQIYGNKWAEIA+FLPGRTDNA+KNHWN + KK+ LDLNL +QS S D SS +K Q + +S + G V +D CST+L +G A + + L G+ + K E+ A + +P +QF + NG+ D + N + + + L+S + + + + + +HP T G M+ S K + G V+ +D +++ S E Q +KK N Y + ++ + + N + +SL+ PG ++ + K N T L K G + PP VKD+ P+ S + I + NS CSTP +SSPES+LRNSAM Y TPSIIRK SR E G+ +P+H + + +D + L ++ SV+GK L RRLEYAFD EWD + RC TPVSA S +GA
Sbjct: 51 TKRSSQAGWTAEEDNLLTEVVKRFKGRNWKGIAECLNGRTDVQCLHRWQKVLNPELVKGPWTKEEDDQIIELVEKFGCKKWSIIAKSLPGRIGKQCRERWHNHLDPTIKRDAWTELEDSVLCRYHQIYGNKWAEIARFLPGRTDNAVKNHWNSTVKKR---LDLNLLSRSGPDIQSEGSPDFSSHKKKLEIKKHLAQAHNAETLSLSKQTG-VDNAADACSTNLRIGCACSPRNDLHKGTSLFGACKSSEKNARDLIKPPGGIQFGKAEILPNGETD---QSNLNLTKISDPLLTSLDFPLDPSRKTRWSSSQVKAVHPTTFGRMYESPKSSKYGTVSD---------TDHDFLTLSLAGF-TEVHSQGNKK--NKAYDIQSSLALKQLGFTEVHSKGNKKN-----KAYDTQSSLVLKQPGFTKIHSRGYKKNKAYDTQSSLAHKQPGFLNYEPPKVKDLTIPLTDENLSRDNIIGRQNSHLLCSTPLSLTVSANSSSPESVLRNSAMSYTKTPSIIRKKTSRFSEASGHYSCTGTTTPMHFL---------RSVPDREDTSNLKDRISGCKRSVSGKSLGRRLEYAFDMEWD--SSRCCTPVSAASPCAIGA 629
Query: 38 AKRSSQAGWTEEEDNRLADVVKKFNGRNWKKIAESLPGRTDVQCLHRWQKVLNPELFKGPWKKEEDDRIIQLVQKYGSRKWSAIAKYLPGRIGKQCRERWHNHLDPAIKKEAWSEREEAILSYYHQIYGNKWAEIAKFLPGRTDNAIKNHWNCSSKKKLDNLDLNL------PLQSPLSDDLSSEANLESVKIPAVQLGVGKAMSTYQIAGPVRKVSDMCSTDLTLGNAYTCESWL--GSKSSRSQKYPEQEAVN--QPLSRVQFRGNSSINNGKMDLPWKRNFHTSNVLASFLSSSSSITEEASGGKTNLAPQGVLHPLTSGGMFASCKRPRNGDVAIFNSEHKSILSDKPWMSCSPGSIDVETPPQLDKKLCNAPYKLVHHFTPSKMVENCERPRSDGSNIINLRRECVQGNSLLNLTPGSSQYSNYIGKGNTVLQT-PKLDAKHYGSFSDGPPYVKDIVAPVRTGEYSSVDDITKANSQSFCSTPSLAQSIVCTSSPESILRNSAMKYEHTPSIIRK--SRPKEG-------GSCSPVHKISMTCESRAANPIDLMDDKTTLHSQLHRSALSVAGKPLERRLEYAFDREWDPLAVRCSTPVSAPSEAVLGA 631
KRSSQAGWT EEDN L +VVK+F GRNWK IAE L GRTDVQCLHRWQKVLNPEL KGPW KEEDD+II+LV+K+G +KWS IAK LPGRIGKQCRERWHNHLDP IK++AW+E E+++L YHQIYGNKWAEIA+FLPGRTDNA+KNHWN + KK+ LDLNL +QS S D SS +K Q + +S + G V +D CST+L +G A + + L G+ + K E+ A + +P +QF + NG+ D + N + + + L+S + + + + + +HP T G M+ S K + G V+ +D +++ S E Q +KK N Y + ++ + + N + +SL+ PG ++ + K N T L K G + PP VKD+ P+ S + I + NS CSTP +SSPES+LRNSAM Y TPSIIRK SR E G+ +P+H + + +D + L ++ SV+GK L RRLEYAFD EWD + RC TPVSA S +GA
Sbjct: 49 TKRSSQAGWTAEEDNLLTEVVKRFKGRNWKGIAECLNGRTDVQCLHRWQKVLNPELVKGPWTKEEDDQIIELVEKFGCKKWSIIAKSLPGRIGKQCRERWHNHLDPTIKRDAWTELEDSVLCRYHQIYGNKWAEIARFLPGRTDNAVKNHWNSTVKKR---LDLNLLSRSGPDIQSEGSPDFSSHKKKLEIKKHLAQAHNAETLSLSKQTG-VDNAADACSTNLRIGCACSPRNDLHKGTSLFGACKSSEKNARDLIKPPGGIQFGKAEILPNGETD---QSNLNLTKISDPLLTSLDFPLDPSRKTRWSSSQVKAVHPTTFGRMYESPKSSKYGTVSD---------TDHDFLTLSLAGF-TEVHSQGNKK--NKAYDIQSSLALKQLGFTEVHSKGNKKN-----KAYDTQSSLVLKQPGFTKIHSRGYKKNKAYDTQSSLAHKQPGFLNYEPPKVKDLTIPLTDENLSRDNIIGRQNSHLLCSTPLSLTVSANSSSPESVLRNSAMSYTKTPSIIRKKTSRFSEASGHYSCTGTTTPMHFL---------RSVPDREDTSNLKDRISGCKRSVSGKSLGRRLEYAFDMEWD--SSRCCTPVSAASPCAIGA 627
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.
There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.
LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.
Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.
RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.
The NCBI Sequence report lists the correspondences between the various naming methods
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.
