MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADS
RIGEQRVRILLAQVRDLVYDAEDVVESFLLKALASERRRSQGKTTLARKI
YNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDK
LVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNV
DVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDYLKEDPMPILEKIP
TLRNLDLTNAYVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPIL
LLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDGEAGPD
FYKVAHIPDLEISGISGELTDAMILKIILGSSDSE*
| Relationships |
|---|
| The polypeptide, DCAR_009477, derives from mRNA, DCAR_009477. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A6I9UNE1 ((putative disease resistance protein At1g50180 {ECO:0000313|RefSeq:XP_011102149.1}))
HSP 1 Score: 219.55 bits (558), Expect = 2.061e-64
Identity = 122/281 (43.42%), Postives = 178/281 (63.35%), Query Frame = 0
Query: 93 KTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDY----------LKEDPMPILEKIPTLRNLDLT-NAYVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDGEAGPDFYKVAHIPDLEI 362
KTTLA KIYN +++ F AWV +SQ++QPK QR+L L+P + E + + LV L ++Q + CL+VLDDIW ++ W+ L+ FP +K++LT RN ++AS R +V+ + LD ++ WELL+ A P +Y ++EDPM ILEK+P LR L L N YVG+EMVC A GF L L L +L NLV+W +EKG MP L L I +C KL+ +P+GL+F+++L+ + I +P +F +RV VDGE G D++K+ HIP ++I
Sbjct: 197 KTTLATKIYNGEAVERCFKYRAWVCVSQQFQPKTTFQRLLKQLLPNESDE---QDENTLVRKLYQVQRDRKCLLVLDDIWEVEHWNCLRRTFPIAEADSKVLLTIRNQNIAS----RGYVHNLKCLDEDEGWELLQKIAFPN-NYSQELQLVDTRIEEDPMKILEKLPMLRVLGLRRNPYVGREMVCEATGFPQLRELSLNDLRNLVEWRVEKGLMPNLSHLVIAKCSKLKMIPDGLEFINTLKTL-IIIIPEEFVKRVGGVDGE-GEDYHKIKHIPFIDI 467
HSP 2 Score: 51.2174 bits (121), Expect = 2.198e-4
Identity = 29/93 (31.18%), Postives = 55/93 (59.14%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDAD---SRIGEQRVRILLAQVRDLVYDAEDVVESFLLKALASERRRS 90
M ++V +I + L D+L +EA L V E+++V +L + FLKDAD R Q V+ +A++R L A++V+E ++++ ++ + +S
Sbjct: 1 MVDSVATIALETLRDILIEEAKFLSSVGGEVEEVRKQLNIMHCFLKDADRRQDRYNSQTVQNWIAELRHLSIQAKNVLERYVIEVVSKRKGKS 93
Match: A0A438D6W3 ((Putative disease resistance protein {ECO:0000313|EMBL:RVW31176.1}))
HSP 1 Score: 197.593 bits (501), Expect = 6.880e-56
Identity = 113/295 (38.31%), Postives = 171/295 (57.97%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFP-NEICR-TKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDY-----------------------------LKEDPMPILEKIPTLRNLDL-TNAYVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDGEAGPDFYKV 354
GKTT+A+K+++H +++HF AW+ ISQ++ ++V+Q IL+ L E +K CL+ LDD+W ID W+SL+ AFP +E R +K++LT+RN VA HV+P F+Y P+ L E SWELL+ KA P+ D L++DPMPILEK+ L L L + Y+GKEM+ S N F L+RLVL + N + ++KG+MP L +LRI C L+ + +GL ++++L+ +EI M + ER++V++G+ G DFYKV
Sbjct: 223 GKTTMAKKVHHHGDVRRHFDCFAWICISQQFNTRSVVQGILMKLATE----------------------EKRCLVTLDDLWEIDDWESLRPAFPLHERGRGSKLVLTTRNQAVALHVDPHGFIYHPKYLTEEDSWELLQSKAYPRHDNNNGRGEISNLPEQHHFPLNLSKLTLISSGLEQDPMPILEKLLNLTILHLFYDVYLGKEMIFSTNEFPRLKRLVLSRISNFKR--LDKGAMPTLKILRIEGCNSLKVVLKGLMYVTTLQVLEIRYMSEELVERLQVLNGKDGEDFYKV 493
Match: A0A397Z256 ((Uncharacterized protein {ECO:0000313|EMBL:RID59757.1}))
HSP 1 Score: 180.259 bits (456), Expect = 5.041e-49
Identity = 115/333 (34.53%), Postives = 180/333 (54.05%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQL-------KKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPK-------------------------------------PDYLKEDP----------------MPILEKIPTLRNLDLTN-AYVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDGEAGPDFYKVAHIPDLEIS 363
GKTTLAR++++H +++HF G AWV +S+++Q K + Q+IL L+ + +V ++ EN L+ +L LIVLDD+W + WD +K AFP E K++LTSR+ V H +P CF + P +L E+SWEL AL + P++ + P +PILEK+ L+++ L++ A+ G++MVCS GF L ++ L L+ L +WEIE+GSMP L L I C KL+E+PEGLK++ SL+++EI GM ++NE++ G +YKV HIP ++++
Sbjct: 205 GKTTLARQVFHHDIVRRHFDGFAWVCVSKEFQRKDIWQKILKDLI------TIDEDVKQMDENELQAKLFLLLGTRTPRHLIVLDDVWKKEDWDRVKDAFPQERG-WKMILTSRDEGVGLHADPTCFAFTPTILTPEESWELCEQIALSRRDKTEFIVDKELEAMGKKMVKYCGGLPLAVKVLGGLLPEHSRFPPKLARISLTKCRMEEDPLPILEKLLHLQSVKLSSGAFAGRKMVCSKGGFPQLHKISLMFLNELEEWEIEEGSMPCLHTLYIGVCGKLKEIPEGLKYIISLKELEILGMNKEWNEKLE----SGGESYYKVQHIPSVQLN 526
HSP 2 Score: 68.1662 bits (165), Expect = 7.662e-10
Identity = 35/82 (42.68%), Postives = 58/82 (70.73%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRIGEQR-VRILLAQVRDLVYDAEDVVESFLLK 81
+AE VV+ V +L DLL++E+ L+GV + + + ++ ++++ LKDAD++ E VR L V+D+VYDAED++ESF+LK
Sbjct: 9 LAEGVVAFGVEKLWDLLSRESERLQGVHEHVADLERQMRKLQSLLKDADAKKHENEVVRNFLEDVKDIVYDAEDIIESFVLK 90
Match: A0A830D1A1 ((Putative disease resistance protein at1g50180 {ECO:0000313|EMBL:GFQ04433.1}))
HSP 1 Score: 179.489 bits (454), Expect = 3.984e-47
Identity = 105/245 (42.86%), Postives = 146/245 (59.59%), Query Frame = 0
Query: 54 EQRVRILLAQVRDLVYDAEDVVESFLLKALASERRRSQGKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNE--ICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDYLKEDPMPILEKIPTLRNLDLTNAYVGKEMVCSANGFQNLERLVLWNL----HNLVKWEI 292
E +++L+A++ D + VV + + L GKTT+ARK+Y H +++HF G AW +SQ+W K VLQ IL+ LVPEKR EIL ++LV+ L ++QLKK CL+VLDDIWS +AW+SLK AFPN +KIMLT+RN +V V+P F+Y+P+VL E+SWELLR KA P+ + +D + I D +GKEMV G L +VL L H L W++
Sbjct: 168 EVDIKLLVARLIDGSKYSNRVVSIYGMGGL--------GKTTIARKLYGHMEVRRHFDGFAWTCVSQQWDKKDVLQGILIKLVPEKRGEILGMRDEELVKQLHDVQLKKKCLVVLDDIWSGEAWESLKPAFPNTRAGAGSKIMLTTRNKEVVDFVSPGGFLYEPRVLSNEESWELLRKKAFPRREDEDDDELKI----------DRDREILGKEMVGRCCGLP-LAIIVLSGLLITKHTLRDWQM 393
HSP 2 Score: 123.635 bits (309), Expect = 5.297e-28
Identity = 63/131 (48.09%), Postives = 85/131 (64.89%), Query Frame = 0
Query: 234 KPDYLKEDPMPILEKIPTLRNLDLT-NAYVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVV-DGEAGPDFYKVAHIPDLEI 362
K L EDPM LEK+P L +L L + Y+G++MVC A GF L L LW L NL W I++G+MP L L I RC KLE LP+GL+F+S+L+K+ + MP +F +R+R G G FYKV H+ D++I
Sbjct: 801 KGSALDEDPMATLEKLPKLSSLTLYESTYMGEDMVCLAQGFPGLLYLKLWGLPNLKTWRIDEGAMPKLTRLVIARCEKLEMLPDGLRFISTLQKLNVRVMPEEFKDRLRTTAAGVEGEHFYKVQHVADIKI 931
HSP 3 Score: 63.5438 bits (153), Expect = 3.700e-8
Identity = 36/88 (40.91%), Postives = 53/88 (60.23%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRI-GEQRVRILLAQVRDLVYDAEDVVESFLLKALASER 87
MAEAVVSI + + +LL E GV E++ + EL R+++FLKDADS+ + V +AQVR+ YD ED++ F +A+ R
Sbjct: 1 MAEAVVSITLETVGNLLVDEGKFYLGVRSEVEAIQLELQRMRSFLKDADSKQDADDTVSNWVAQVREAAYDIEDILVVFAAAKVATRR 88
Match: A0A2G9G4I5 ((Apoptotic ATPase {ECO:0000313|EMBL:PIN00218.1}))
HSP 1 Score: 176.792 bits (447), Expect = 3.141e-46
Identity = 100/209 (47.85%), Postives = 133/209 (63.64%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRT----KIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALP-KPDYLKEDPMPILEKIPTLRNLDLTNAYVGKEMVCSANGFQNLERLVLWNL----HNLVKWE 291
GKTT+ARK+Y+H +++HF G AW ISQ+W K ++Q IL+ L+PE+R +IL D+LV+ L ++Q+KK CL+VLDD+WS +AW+SLK AFPN RT KI+LT+RN +VAS VNP F+Y+P+VL E+SWELLR KA P +P+ E + P + NL GKEMV G L LVL L H L W
Sbjct: 195 GKTTIARKLYSHRDVRRHFDGFAWTCISQQWDKKDLMQGILIKLIPEERDKILGMRDDELVKELHDVQVKKKCLVVLDDVWSGEAWESLKHAFPN--TRTGEGSKILLTTRNREVASFVNPSGFLYEPRVLSTEESWELLRKKAFPRRPNDASEFKID-----PEMENL-------GKEMVGKCRGLP-LAILVLSGLLITKHTLRDWH 388
HSP 2 Score: 127.872 bits (320), Expect = 1.841e-29
Identity = 65/130 (50.00%), Postives = 85/130 (65.38%), Query Frame = 0
Query: 234 KPDYLKEDPMPILEKIPTLRNLDLTNA-YVGKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDGEAGPDFYKVAHIPDLEI 362
K L EDPM LEK+P L +L L ++ YVG++MVC GF L L LW L N W IE+G+MP L L I C KLE LP+GLKF+++L+K+ + MP DF R+R D E G DFYKV H+PD+++
Sbjct: 788 KASALGEDPMATLEKLPKLSSLILYDSTYVGEDMVCLTKGFPRLLYLKLWGLSNWKTWRIEEGAMPKLTRLVIAHCDKLEMLPDGLKFITTLQKLNVRSMPGDFKYRLRKADRE-GEDFYKVRHVPDIKL 916
HSP 3 Score: 74.7146 bits (182), Expect = 8.722e-12
Identity = 42/91 (46.15%), Postives = 58/91 (63.74%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRI-GEQRVRILLAQVRDLVYDAEDVVESFLLKALASERRRS 90
MAEAVVSI + L +L +EA GV E++ + EL R+++FLKDADSR E+RV +A+VR+ YD ED V F+ +AS R R+
Sbjct: 1 MAEAVVSITLETLRSILVEEAKFYYGVRSEVESIQQELQRMRSFLKDADSRQDAEERVSNWVAEVREAAYDIEDNVLVFIAAKIASRRSRN 91
Match: A0A5B7BM36 (NB-ARC domain-containing protein {ECO:0000259|Pfam:PF00931} (Fragment))
HSP 1 Score: 165.622 bits (418), Expect = 4.671e-46
Identity = 77/145 (53.10%), Postives = 106/145 (73.10%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFP-NEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKP 235
GKTT+A+K+YNH IK HF G AWV ISQ+WQ + VLQRIL+ L+P +R +I ++LV L ++Q+ K CL+VLDDIWS+DAWD LK AF N TK++LT+RN +VA HV+P F+++P+ L+ ++SWELL+ KA K
Sbjct: 62 GKTTIAQKVYNHMKIKHHFDGFAWVCISQQWQTRHVLQRILIKLIPHEREKIEKMGEEELVGLLYQVQMNKKCLVVLDDIWSMDAWDCLKHAFSINGKMGTKLLLTTRNQEVALHVDPNGFLHEPRFLNEDESWELLQKKAFAKT 206
Match: A0A7J7N4Z3 ((Uncharacterized protein {ECO:0000313|EMBL:KAF6161978.1}))
HSP 1 Score: 164.081 bits (414), Expect = 8.403e-45
Identity = 118/337 (35.01%), Postives = 172/337 (51.04%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSR-IGEQRVRILLAQVRDLVYDAEDVVESFLLKALASERRRS--------------------------------QGKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDYLKEDPMPILEKIPTLRNLDLTNAYVGKEMVCSANGFQ---NLERLVLWNLHNLVKWE-IEKGSMPIL 300
MA+ VVS V+ RL L +E LL GVT+++QQ++TE+ R++ FL +AD+R I ++ VR +A+ R L D EDV++ ++ K +AS RR GKTTLA+K+YNH +K+HF AW +SQK++ +AVL +I + I E + +VE L + K +VLDDIW+ DAW LK A PN +K++ T+R ++VA +PRC ++P++L +SWELLR K L + T RN +GK+MV G + R +L L WE I K MP L
Sbjct: 1 MAQPVVSFVIKRL---LIEEVVLLSGVTEQVQQMLTEMKRMECFLNEADARQIEDELVRNWVAEFRKLANDEEDVIDYYIYK-VASRRRGGIPAFLKSTNTLVEELKKKERQLCVVSIVGIGGLGKTTLAKKVYNHRRVKRHFHCHAWACMSQKFKRRAVLHQISKQFGYKVEQSI---EENDIVEGLRKFMENKRYFLVLDDIWNEDAWAILKPALPNGKSGSKVLFTTRFMNVAVAADPRCLHFEPRLLSEVESWELLRKKVLLQ---------------DTDRNCSPYLVKLGKKMVEKCGGLPLAVGVLRGLLARKMTLYDWEIINKSLMPQL 315
Match: A0A5B6ZGX6 ((Uncharacterized protein {ECO:0000313|EMBL:MPA43235.1}) (Fragment))
HSP 1 Score: 164.466 bits (415), Expect = 9.068e-45
Identity = 74/142 (52.11%), Postives = 103/142 (72.54%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALP 233
GKTTLA K+Y+HS +++HF AW ISQ+WQ K VL+ +L L+P +R +I D+LV+ L ++Q +K CL+VLDDIWS DAWDSL AFPN +KI+LTSRN +VA H +P ++++P+ L+ E+SWELL+ KA P
Sbjct: 196 GKTTLAEKVYHHSDVRRHFDSFAWFCISQQWQKKDVLKGLLFKLIPNEREQIKEMIDDELVKRLYDVQRQKKCLVVLDDIWSTDAWDSLSPAFPNRETGSKILLTSRNKEVALHRDPNVYLHEPKCLNQEESWELLQKKAFP 337
HSP 2 Score: 64.3142 bits (155), Expect = 9.247e-9
Identity = 34/74 (45.95%), Postives = 50/74 (67.57%), Query Frame = 0
Query: 9 VVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRIGE-QRVRILLAQVRDLVYDAEDVVESFLLK 81
VV R+ DLL EA L GV ++++V TEL R++ FLKDAD+R E + ++ + ++R++ YD EDVVE F K
Sbjct: 1 VVERVGDLLINEAKFLCGVRGKVEEVQTELRRMQCFLKDADARQDESEMIKNWVEEIREIAYDVEDVVEIFTFK 74
Match: A0A6P6SSZ8 ((probable disease resistance protein RF9 {ECO:0000313|RefSeq:XP_027069038.1}))
HSP 1 Score: 171.785 bits (434), Expect = 2.287e-44
Identity = 92/177 (51.98%), Postives = 117/177 (66.10%), Query Frame = 0
Query: 92 GKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKAL-PKPDYLKEDPMPILEKIPTLRNLDLTNAYVGKEMV 267
GKTTLARK+YNH +++ F GLAWV +SQKWQ + +LQRIL+SL+PEKR EIL ++LV L +I KNCL+VLDD+WS DAWD +K AFP KI++TSRN DVA H++P F ++P++L +SWELL+ KAL K D ED L NL GKEMV
Sbjct: 201 GKTTLARKVYNHPKVRRCFDGLAWVCVSQKWQKEDILQRILLSLIPEKRKEILEWRDEELVRQLFQILQNKNCLVVLDDMWSADAWDCIKQAFPIRKNGYKILVTSRNKDVALHIDPNGFHHQPRLLSDSESWELLQRKALRHKFDTEHED-------FKKLENL-------GKEMV 363
HSP 2 Score: 109.768 bits (273), Expect = 2.328e-23
Identity = 67/179 (37.43%), Postives = 104/179 (58.10%), Query Frame = 0
Query: 195 LTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDYLKEDPMPILEKIPTLRNLDLTNAYV-GKEMVCSANGFQNLERLVLWNLHNLVKWEIEKGSMPILLLLRIRRCRKLEELPEGLKFLSSLEKIEINGMPSDFNERVRVVDG--EAGPDFYKVAHIPDLEISGISGELT 370
+SRN+ V P P+ + LL+LK L + + ++E M LE++P LR+L L + Y+ GKEM C A GF L L L NL KW +++G+MP L +L I C KLE +P GL+F+ +L+++ I MP +F +R++ +G + G DF KV+HIP + + +S L+
Sbjct: 816 FSSRNLQVLKVSGP--LANFPKYEAQYRYASLLKLK-LTRME-IEEYSMETLERLPNLRSLHLQDLYILGKEMRCKATGFGQLRFLRFECLWNLEKWNVDEGAMPNLSVLTIVYCTKLEMVPNGLRFVKTLKELSIAAMPKEFTDRIQAANGHEDQGQDFDKVSHIPTIIVRNVSNILS 990
HSP 3 Score: 72.7886 bits (177), Expect = 4.174e-11
Identity = 39/91 (42.86%), Postives = 62/91 (68.13%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRIGE-QRVRILLAQVRDLVYDAEDVVESFLLKALASERRRS 90
MAEA VS V + +LL +EA L GV+D+++Q+ L R+++FLKDAD E + ++ ++Q RDL Y+A+D++ES+ K +AS RR+
Sbjct: 1 MAEAAVSFAVETIGNLLIEEARFLDGVSDQVEQLQLTLKRMQSFLKDADEMQHEGEMLKEWISQARDLAYEADDLIESYAFK-VASRRRKG 90
Match: A0A5J5C5G7 (AAA domain-containing protein {ECO:0000259|SMART:SM00382})
HSP 1 Score: 164.081 bits (414), Expect = 4.251e-44
Identity = 81/154 (52.60%), Postives = 113/154 (73.38%), Query Frame = 0
Query: 90 SQGKTTLARKIYNHSTIKKHFSGLAWVSISQKWQPKAVLQRILVSLVPEKRAEILHSEVDKLVENLLEIQLKKNCLIVLDDIWSIDAWDSLKAAFPNEICRTKIMLTSRNVDVASHVNPRCFVYKPQVLDAEQSWELLRLKALPKPDYLKEDPM 243
GKTTLARK+YN+ ++++F G AWV ISQ+W+ + V++RIL+ LVPEK+ EI ++L+ LLE+Q KK CL+VLDDIWS+DAWD LK AFP TK++LT+RN DVASHV+P F+++ ++LD + WELL+ KA K L++D M
Sbjct: 197 GSGKTTLARKVYNNIDVQRYFEGFAWVCISQQWEKEDVMRRILIKLVPEKKEEIYKMTDEELIRLLLEVQ-KKKCLVVLDDIWSMDAWDCLKPAFPIGKMGTKLLLTTRNKDVASHVDPEGFLHE-RLLDENEGWELLQKKAFTKT-ALRDDKM 347
HSP 2 Score: 77.0258 bits (188), Expect = 7.488e-13
Identity = 49/124 (39.52%), Postives = 74/124 (59.68%), Query Frame = 0
Query: 1 MAEAVVSIVVGRLTDLLTQEATLLRGVTDEIQQVVTELMRIKAFLKDADSRIGE-QRVRILLAQVRDLVYDAEDVVESFLLKALASERRRSQGKTTLARKIYN--HSTIKKHFSGLAWVSISQK 121
+AE VVS VV RL DLL EA L GV+ ++QQ+ T+L R++ FL+DAD++ E +RV + ++R++ Y+ EDV+E+F+L+ RR K L R + I +H G SI +K
Sbjct: 4 LAEPVVSFVVERLGDLLITEAKLSHGVSGQVQQIQTQLSRMQCFLRDADAKQDEGERVCNWIVEIREIAYETEDVIETFVLR--VGSRRGGGVKIILHRYMGCCLKECIARHKIGSEIQSIKRK 125
| Match Name | Stats | Description |
|---|---|---|
| A0A6I9UNE1 | E-Value: 2.061e-64, PID: 43.42 | (putative disease resistance protein At1g50180 {EC... [more] |
| A0A438D6W3 | E-Value: 6.880e-56, PID: 38.31 | (Putative disease resistance protein {ECO:0000313|... [more] |
| A0A397Z256 | E-Value: 5.041e-49, PID: 34.53 | (Uncharacterized protein {ECO:0000313|EMBL:RID5975... [more] |
| A0A830D1A1 | E-Value: 3.984e-47, PID: 42.86 | (Putative disease resistance protein at1g50180 {EC... [more] |
| A0A2G9G4I5 | E-Value: 3.141e-46, PID: 47.85 | (Apoptotic ATPase {ECO:0000313|EMBL:PIN00218.1})[more] |
| A0A5B7BM36 | E-Value: 4.671e-46, PID: 53.10 | NB-ARC domain-containing protein {ECO:0000259|Pfam... [more] |
| A0A7J7N4Z3 | E-Value: 8.403e-45, PID: 35.01 | (Uncharacterized protein {ECO:0000313|EMBL:KAF6161... [more] |
| A0A5B6ZGX6 | E-Value: 9.068e-45, PID: 52.11 | (Uncharacterized protein {ECO:0000313|EMBL:MPA4323... [more] |
| A0A6P6SSZ8 | E-Value: 2.287e-44, PID: 51.98 | (probable disease resistance protein RF9 {ECO:0000... [more] |
| A0A5J5C5G7 | E-Value: 4.251e-44, PID: 52.60 | AAA domain-containing protein {ECO:0000259|SMART:S... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |