Resource Type:
Polypeptide
Name:
DCAR_025679
Identifier:
DCAR_025679.mRNA-protein
Sequence:
MKLASGSIHPAKAQRFFKIIVSEISPQSKLMIPKEFVKNHEEELRGRVHL
KAPGSAAWPVDVVRTQGEVWLQNGWPEFAKFYSLSFGSLLVFEYLEHFNI
RVRVFDTSATEIDYSLTCDPNLDDGESVPRVRKRDSSRRCELVRACKKTR
ASTEETFHNGFKLKQEKANGDRGSAKEEIERAVNLAASFKSEKPFFIIHM
RPTYVSGVSVEVRKSFLETFRKWKNKGKGQVIFQIGRRSWLVGYTFSAGD
RCTLTAGWKTFRTDNSLKVDDVCVFELINPSTKLFKVIIFQAKSRPDEYI
CDVCSRLVKFAYACEECTVDVCVFCGLEQRVLCHEGHQQHKLTLMNREAL
FKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYS
IPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVT
DTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQGEGESSTPIP
DNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITD
THPSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQFYG
YVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLVQRP
FSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVT
LREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKF
GGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGC
GFLVCCNCFGEITDSTNSTSGSGSVSC*
Sequence Length:
828
Sequence Checksum:
8912783ea1972ffce6222cc93cf3e439
View location in JBrowse:
Organism:
Relationship:
| Relationships |
|---|
| The polypeptide, DCAR_025679, derives from mRNA, DCAR_025679. |
Blast Results:
The following BLAST results are available for this feature:
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7ABQ0 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 273.092 bits (697), Expect = 1.609e-78
Identity = 192/554 (34.66%), Postives = 273/554 (49.28%), Query Frame = 0
HSP 2 Score: 78.1814 bits (191), Expect = 3.174e-12
Identity = 66/200 (33.00%), Postives = 91/200 (45.50%), Query Frame = 0
Match: A0A5B7ABQ0 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 273.092 bits (697), Expect = 1.609e-78
Identity = 192/554 (34.66%), Postives = 273/554 (49.28%), Query Frame = 0
Query: 301 CDVCSRLVK-FAYACEECTVDVCVFCGLE--QRVLCHEGHQQHKLTLMNREALFKCDACGEEDKD-SSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDT----EAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQ-GEGESSTPI---PDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQC-GFFLHSFCATKLPPELPRGAS-SFHPSHSLVLYM----SDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRH----------------------SLVQRPFSN-SICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYE---SFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNCF 809
C VC K F Y C +C +C+ C LE +R + H G QH L L+N A F CDAC + +D SSYVCT C FWIH+ CA P + T + HHHPL L YS+P + +FE C IC + V W+YYC +C YF H+ CA S +D+ EA D+D L+Q P P + DLI F L GE ES+T + D+ ++ H+SH++ + D+ +D +++C+GC+ PI+ + +Y+C QC FFLH CA +LP+ S FH H L L + Y C +C NGF + C C+ +D++CA L IKH +HKRH +V+ FS+ ++C+V + G+ CETC + C + P + HRYD H + L PPF + FYCEICEE+++ +YHC +CD SFH C+ E +FKFG + PH L +V R+ C C + +++ FEC C +VC C
Sbjct: 89 CTVCQESWKEFTYHCSDCKFHLCISCSLEVKERRIVH-GSHQHPLNLLNMPASFNCDACNIKSEDTSSYVCTACPFWIHERCASLPSTM-TSSAHHHPLTLAYSLPLKYSKFEYRCQICSKRVVPTYWVYYCPRCRYFAHVNCAKSETGSVRSSDSENEIEAKDLDPN--LIQLPPP-PQKYIDLI----SHFPEKLNSGENESATEVNHLSDDQDQLK-HFSHDHPLILSDVVQLQGSSKDEIQNDEIIILCDGCVLPISISAAPFYSCAQCPSFFLHKCCA-----QLPKMVSHPFHTKHQLSLSLHGNGDSDIADYFTCNLCKVICNGFRFNCVPCNFSLDVKCASLLGTIKHEAHKRHLIQFKSDDLPDFSSTGIFSFVGQVVKNFFSSYNLCNVCGENFS-GLSLGCETCDFSLCNHHCAMLPPTVRHRYDEHPLYLSYPPFQDHPDEFYCEICEEEIDPNLWMYHCRDCDQSFHTKCIYKVEETLNFKFGETFK--ARNHPHPLTVV-----RENKRQSWCDRCSKYFDYKPAFECAPCNTIVCHQCI 619
HSP 2 Score: 78.1814 bits (191), Expect = 3.174e-12
Identity = 66/200 (33.00%), Postives = 91/200 (45.50%), Query Frame = 0
Query: 553 PSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMS----DQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPK--RIKHRSHKRH-SLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLR-EPPFFYEGVFY-CEICEEQVNNQRLLYHCDECDHSFHHYCLR 743
P+Y QC F LH CA +LP ++ HP HSL+L++ D + Y +C VC F Y C DC + I C+ K RI H SH+ +L+ P S + C N+K + Y C C F IH C PS M H +TL P Y Y C+IC ++V +Y+C C + H C +
Sbjct: 38 PAYICSQQCSFHLHKMCA-ELPEKIEHPK---HPQHSLILHIEPYDIDCSWEYKECTVCQESWKEFTYHCSDCKFHLCISCSLEVKERRIVHGSHQHPLNLLNMPASFN-CDACNIKSEDTSSYVCTAC-PFWIHERCASLPSTMTSSAHHHPLTLAYSLPLKYSKFEYRCQICSKRVVPTYWVYYCPRCRYFAHVNCAK 231
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7ABP9 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081} (Fragment))
HSP 1 Score: 272.322 bits (695), Expect = 4.307e-78
Identity = 193/553 (34.90%), Postives = 276/553 (49.91%), Query Frame = 0
HSP 2 Score: 78.1814 bits (191), Expect = 3.389e-12
Identity = 66/200 (33.00%), Postives = 91/200 (45.50%), Query Frame = 0
Match: A0A5B7ABP9 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081} (Fragment))
HSP 1 Score: 272.322 bits (695), Expect = 4.307e-78
Identity = 193/553 (34.90%), Postives = 276/553 (49.91%), Query Frame = 0
Query: 301 CDVCSRLVK-FAYACEECTVDVCVFCGLE--QRVLCHEGHQQHKLTLMNREALFKCDACGEEDKD-SSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDT----EAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQ-GEGESSTPI---PDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQC-GFFLHSFCATKLPPELPRGAS-SFHPSHSLVLYM----SDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRH----------------------SLVQRPFSN-SICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYE---SFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNC 808
C VC K F Y C +C +C+ C LE +R + H G QH L L+N A F CDAC + +D SSYVCT C FWIH+ CA P + T + HHHPL L YS+P + +FE C IC + V W+YYC +C YF H+ CA S +D+ EA D+D L+Q P P + DLI F L GE ES+T + D+ ++ H+SH++ + D+ +D +++C+GC+ PI+ + +Y+C QC FFLH CA +LP+ S FH H L L + Y C +C NGF + C C+ +D++CA L IKH +HKRH +V+ FS+ ++C+V + G+ CETC + C + P + HRYD H + L PPF + FYCEICEE+++ +YHC +CD SFH C+ E +FKFG + PH L +V + A+ +S C C + +++ FEC C +VC C
Sbjct: 111 CTVCQESWKEFTYHCSDCKFHLCISCSLEVKERRIVH-GSHQHPLNLLNMPASFNCDACNIKSEDTSSYVCTACPFWIHERCASLPSTM-TSSAHHHPLTLAYSLPLKYSKFEYRCQICSKRVVPTYWVYYCPRCRYFAHVNCAKSETGSVRSSDSENEIEAKDLDPN--LIQLPPP-PQKYIDLI----SHFPEKLNSGENESATEVNHLSDDQDQLK-HFSHDHPLILSDVVQLQGSSKDEIQNDEIIILCDGCVLPISISAAPFYSCAQCPSFFLHKCCA-----QLPKMVSHPFHTKHQLSLSLHGNGDSDIADYFTCNLCKVICNGFRFNCVPCNFSLDVKCASLLGTIKHEAHKRHLIQFKSDDLPDFSSTGIFSFVGQVVKNFFSSYNLCNVCGENFS-GLSLGCETCDFSLCNHHCAMLPPTVRHRYDEHPLYLSYPPFQDHPDEFYCEICEEEIDPNLWMYHCRDCDQSFHTKCIYKVEETLNFKFGETFK--ARNHPHLLTVV--QEAKRQS---WCDRCNQYFDYKPAFECVPCNTMVCIRC 640
HSP 2 Score: 78.1814 bits (191), Expect = 3.389e-12
Identity = 66/200 (33.00%), Postives = 91/200 (45.50%), Query Frame = 0
Query: 553 PSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMS----DQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPK--RIKHRSHKRH-SLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLR-EPPFFYEGVFY-CEICEEQVNNQRLLYHCDECDHSFHHYCLR 743
P+Y QC F LH CA +LP ++ HP HSL+L++ D + Y +C VC F Y C DC + I C+ K RI H SH+ +L+ P S + C N+K + Y C C F IH C PS M H +TL P Y Y C+IC ++V +Y+C C + H C +
Sbjct: 60 PAYICSQQCSFHLHKMCA-ELPEKIEHPK---HPQHSLILHIEPYDIDCSWEYKECTVCQESWKEFTYHCSDCKFHLCISCSLEVKERRIVHGSHQHPLNLLNMPASFN-CDACNIKSEDTSSYVCTAC-PFWIHERCASLPSTMTSSAHHHPLTLAYSLPLKYSKFEYRCQICSKRVVPTYWVYYCPRCRYFAHVNCAK 253
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6WHB0 ((uncharacterized protein LOC113732809 {ECO:0000313|RefSeq:XP_027114595.1}))
HSP 1 Score: 266.544 bits (680), Expect = 2.915e-76
Identity = 181/543 (33.33%), Postives = 260/543 (47.88%), Query Frame = 0
HSP 2 Score: 45.8246 bits (107), Expect = 3.125e-2
Identity = 54/205 (26.34%), Postives = 81/205 (39.51%), Query Frame = 0
Match: A0A6P6WHB0 ((uncharacterized protein LOC113732809 {ECO:0000313|RefSeq:XP_027114595.1}))
HSP 1 Score: 266.544 bits (680), Expect = 2.915e-76
Identity = 181/543 (33.33%), Postives = 260/543 (47.88%), Query Frame = 0
Query: 292 AKSRPDEYICDVCSRLVK-FAYACEECTVDVCVFCG---LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADIDDETVLLQF-PLPHEESLFDLI---ITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLVQ-----RPFSNS-----ICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYC---LRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNCFGEI 812
AK + C C + ++ F + C C D+CV C L++R L H+ H H LT M R A F CD C E+ +D S+ C C +WIH++CA P + H HPL L Y +P R C++C + W Y C+ C +FVH+ C + ++ + L F PLP + DLI + + G ++ L + H+SHE+ QL + + D D N+++ M+C+ CI+PI+ YY C QC F LH CA +P ++ A HP H L L + YGY C C NGF+++CE C+ +ID+ CAFLP+ I H+ H +H L + P ++ S KI+ + Y+C+ C +H C I P + HR+D H +TL PPF + FYCEIC E++N + LYHC ECD SFH YC LR KFG + +Q H L R R+ S C C + FECG C F +C C ++
Sbjct: 89 AKPPYTKCFCSSCGKFMESFTFNCSFCKFDLCVECAHLVLQKRRLKHDSHH-HFLTSMQRPATFSCDGCDEKKEDWSFSCNICPYWIHRSCAFLP-TTKKRDDHEHPLSLAYHLPFDLRLNHFNCDVCRRPLNPSRWFYQCYSCKHFVHVGCVQDGTKPAGAKGSSSSAGNQPDHGLDFKPLPWTDMSADLIRPFLEKMGDVEIQLTNQI--------------NHFSHEH----QLILSVAEVDKDKNEEE---MLCDACIEPISTP---YYTCSQCNFLLHLNCAN-IPAKM--KAHHDHPEHQLSLRKLENKYGYFYCNFCFLNCNGFFFECELCNYRIDLHCAFLPRTITHQIHDQHVLNRNIAPMEPLCDACGNKFFGDASEKKISGLIRYSCDDCFKLSLHYQCAIMPHTVAHRWDKHCLTLMYPPFGDHPDEFYCEICGEEINPKHWLYHCKECDQSFHLYCIPRLRKDRFMKFG----KTLQVSGHQHPLTSVRECRNGSS---CNRCSTKLSGGKGFECGKCKFFLCYKCACDV 595
HSP 2 Score: 45.8246 bits (107), Expect = 3.125e-2
Identity = 54/205 (26.34%), Postives = 81/205 (39.51%), Query Frame = 0
Query: 629 CAFLPKRIKHRSHKRHS---LVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDC---VIYPSRMNHRYDLHAVTLREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYN-FLFFFECGGCGFLVCCNCFGEITDSTNSTSGSGSVS 826
CA LP+ IKH H H L + P++ CS S K + C C F + ++C V+ R+ H H +T + P F C+ C+E+ + + C+ C + H C + K R E P +LA L R + C +C N +F++C C V C + D T GS S
Sbjct: 68 CANLPREIKHPIHAEHPFTLLAKPPYTKCFCS-SCGKFMESFTFNCSFC-KFDLCVECAHLVLQKRRLKHDSHHHFLTSMQRP----ATFSCDGCDEKKEDWS--FSCNICPYWIHRSCAFLPTTKK------RDDHEHPLSLAYHLPFDLRLNH--FNCDVCRRPLNPSRWFYQCYSCKHFVHVGC---VQDGTKPAGAKGSSS 253
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6VYH3 ((uncharacterized protein LOC113727606 {ECO:0000313|RefSeq:XP_027107665.1}))
HSP 1 Score: 265.003 bits (676), Expect = 1.017e-75
Identity = 183/544 (33.64%), Postives = 260/544 (47.79%), Query Frame = 0
HSP 2 Score: 43.5134 bits (101), Expect = 1.732e-1
Identity = 51/192 (26.56%), Postives = 78/192 (40.62%), Query Frame = 0
Match: A0A6P6VYH3 ((uncharacterized protein LOC113727606 {ECO:0000313|RefSeq:XP_027107665.1}))
HSP 1 Score: 265.003 bits (676), Expect = 1.017e-75
Identity = 183/544 (33.64%), Postives = 260/544 (47.79%), Query Frame = 0
Query: 292 AKSRPDEYICDVCSRLVK-FAYACEECTVDVCVFCG---LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADIDDETVLLQF-PLPHEESLFDLI---ITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLVQRPFSNSIC-----------SVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYC---LRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNCFGEI 812
AK + C C + ++ F + C C D+CV C L++R L H+ H H LT M R A F CD C E+ +D S+ C C +WIH++CA P + H HPL L Y +P R C++C + W Y C+ C +FVH+ C + ++ + L F PLP + DLI + + G ++ L + H+SHE+ QL + + D D+N+++ M+C+ CI+PI+ YY C QC F LH CA +P ++ A HP H L L + YGY C C NGF+++CE C+ +ID+ CAFLP+ I H+ H +H L R + C S KI+ V Y+C+ C +H C I P + HR+D H +TL PPF + FYCEIC E++N + LYHC ECD SFH YC LR KFG + +Q H L R R+ S C C + FECG C F +C C ++
Sbjct: 89 AKPPYTKCFCSSCGKFMEGFTFNCSFCKFDLCVECAHLVLQKRRLKHDSHH-HFLTSMQRPATFSCDGCDEKKEDWSFSCNICPYWIHRSCAFLP-TTKKRDDHEHPLSLAYHLPFDLRLNHFNCDVCRRPLNPSRWFYQCYSCKHFVHVGCVQDGTKPAGAKGSSSSASNQPDHGLDFKPLPWTDMSADLIRPFLEKMGDVEIQLTNQI--------------NHFSHEH----QLILSVAEVDKDNNEEE---MLCDACIEPIST---PYYTCSQCNFLLHLNCAN-IPAKM--KAHHDHPEHQLSLRKLENKYGYFYCNFCFLNCNGFFFECEPCNYRIDLHCAFLPRTITHQIHDQHVL-NRNIAPMECLCDACGNKFFGDASEKKISGLVRYSCDDCFKLSLHYQCAIMPHTVAHRWDKHCLTLMYPPFGDHPDEFYCEICGEEINPKHWLYHCKECDQSFHLYCIPRLRKDRFMKFG----KTLQVSDHQHPLTSIRECRNGSS---CNRCSTKLSGGKGFECGKCKFFLCYKCACDV 595
HSP 2 Score: 43.5134 bits (101), Expect = 1.732e-1
Identity = 51/192 (26.56%), Postives = 78/192 (40.62%), Query Frame = 0
Query: 629 CAFLPKRIKHRSHKRHS---LVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDC---VIYPSRMNHRYDLHAVTLREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYN-FLFFFECGGCGFLVCCNCFGEIT 813
CA LP+ IKH H H L + P++ CS S K G + C C F + ++C V+ R+ H H +T + P F C+ C+E+ + + C+ C + H C + K R E P +LA L R + C +C N +F++C C V C + T
Sbjct: 68 CANLPREIKHPIHAEHPFTLLAKPPYTKCFCS-SCGKFMEGFTFNCSFC-KFDLCVECAHLVLQKRRLKHDSHHHFLTSMQRP----ATFSCDGCDEKKEDWS--FSCNICPYWIHRSCAFLPTTKK------RDDHEHPLSLAYHLPFDLRLNH--FNCDVCRRPLNPSRWFYQCYSCKHFVHVGCVQDGT 243
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A068V412 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 264.233 bits (674), Expect = 1.532e-75
Identity = 183/544 (33.64%), Postives = 259/544 (47.61%), Query Frame = 0
HSP 2 Score: 43.5134 bits (101), Expect = 1.688e-1
Identity = 51/192 (26.56%), Postives = 78/192 (40.62%), Query Frame = 0
Match: A0A068V412 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 264.233 bits (674), Expect = 1.532e-75
Identity = 183/544 (33.64%), Postives = 259/544 (47.61%), Query Frame = 0
Query: 292 AKSRPDEYICDVCSRLVK-FAYACEECTVDVCVFCG---LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADIDDETVLLQF-PLPHEESLFDLI---ITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLVQRPFSNSIC-----------SVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYC---LRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNCFGEI 812
AK + C C + ++ F + C C D+CV C L++R L H+ H H LT M R A F CD C E+ +D S+ C C +WIH++CA P + H HPL L Y +P R C++C + W Y C+ C +FVH+ C + ++ + L F PLP + DLI + + G ++ L + H+SHE+ QL + + D D N+++ M+C+ CI+PI+ YY C QC F LH CA +P ++ A HP H L L + YGY C C NGF+++CE C+ +ID+ CAFLP+ I H+ H +H L R + C S KI+ V Y+C+ C +H C I P + HR+D H +TL PPF + FYCEIC E++N + LYHC ECD SFH YC LR KFG + +Q H L R R+ S C C + FECG C F +C C ++
Sbjct: 89 AKPPYTKCFCSSCGKFMEGFTFNCSFCKFDLCVECAHLVLQKRRLKHDSHH-HFLTSMQRPATFSCDGCDEKKEDWSFSCNICPYWIHRSCAFLP-TTKKRDDHEHPLSLAYHLPFDLRLNHFNCDVCRRPLNPSRWFYQCYSCKHFVHVGCVQDGTKPAGAKGSSSSASNQPDHGLDFKPLPWTDMSADLIRPFLEKMGDVEIQLTNQI--------------NHFSHEH----QLILSVAEVDKDKNEEE---MLCDACIEPIST---PYYTCSQCNFLLHLNCAN-IPAKM--KAHHDHPEHQLSLRKLENKYGYFYCNFCFLNCNGFFFECEPCNYRIDLHCAFLPRTITHQIHDQHVL-NRNIAPMECLCDACGNKFFGDASEKKISGLVRYSCDDCFKLSLHYQCAIMPHTVAHRWDKHCLTLMYPPFGDHPDEFYCEICGEEINPKHWLYHCKECDQSFHLYCIPRLRKDRFMKFG----KTLQVSDHQHPLTSIRECRNGSS---CNRCSTKLSGGKGFECGKCKFFLCYKCACDV 595
HSP 2 Score: 43.5134 bits (101), Expect = 1.688e-1
Identity = 51/192 (26.56%), Postives = 78/192 (40.62%), Query Frame = 0
Query: 629 CAFLPKRIKHRSHKRHS---LVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDC---VIYPSRMNHRYDLHAVTLREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYN-FLFFFECGGCGFLVCCNCFGEIT 813
CA LP+ IKH H H L + P++ CS S K G + C C F + ++C V+ R+ H H +T + P F C+ C+E+ + + C+ C + H C + K R E P +LA L R + C +C N +F++C C V C + T
Sbjct: 68 CANLPREIKHPIHAEHPFTLLAKPPYTKCFCS-SCGKFMEGFTFNCSFC-KFDLCVECAHLVLQKRRLKHDSHHHFLTSMQRP----ATFSCDGCDEKKEDWS--FSCNICPYWIHRSCAFLPTTKK------RDDHEHPLSLAYHLPFDLRLNH--FNCDVCRRPLNPSRWFYQCYSCKHFVHVGCVQDGT 243
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2G9HES0 ((Uncharacterized protein {ECO:0000313|EMBL:PIN16006.1}))
HSP 1 Score: 257.299 bits (656), Expect = 8.448e-73
Identity = 179/540 (33.15%), Postives = 268/540 (49.63%), Query Frame = 0
Match: A0A2G9HES0 ((Uncharacterized protein {ECO:0000313|EMBL:PIN16006.1}))
HSP 1 Score: 257.299 bits (656), Expect = 8.448e-73
Identity = 179/540 (33.15%), Postives = 268/540 (49.63%), Query Frame = 0
Query: 299 YICDVCSRLVK-FAY---ACEECTVDVCVFCGL------EQRVLCHEGHQQHKLTLMNR-EALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQF----ATSNDDNDD-NDDDRRVMICNGCIQPITDTHPS--YYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLV-----QRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPF-FYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLR---WYESFKFG-GIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNCFG 810
Y C C RL K FAY + + + + CG EQR + H H+ H LT + + +LF CD CG E++D +YVC CEFWIHK+C L P + ++H H L L Y +P ++ +F C+ICH +Y + W+Y+C C +F H++CA + T I + + ++ L+Q P+ + DLI+ + +V + ST IP ++ H H L + F AT++ + + DD+ +M+C+ CI PI + P YY C +C FFLH C LP EL H L L M + DC+ C TNG +Y+CEDC ID++CA + I+H ++++ L+ QR F + CS I+ YA E F +H +C + P H+ D H ++L PP+ + G YCEICE++++ +R Y+C ECD FH YCL WY + KFG ++ + H+L L S + C +C FEC C F C C G
Sbjct: 86 YYCCNCWRLCKGFAYLGSSSDGLEFTISISCGCLDYLLEEQRSIKHPSHR-HPLTYVRKPSSLFHCDGCGTEERDFAYVCNFCEFWIHKSCTLLPTTFQSVSHHQHLLSLNYFLPHVYHRFRFNCDICHHRIYPMYWVYFCASCRFFAHVKCA-TQQTNSIAGPSNSNTEQEDGSLIQLPVNKVST--DLIVPFLIRQKVLPDIDDALSTTIPTTNQPQYFNYEHHLHPLTLVIFSNEVATADGEQINVTDDEGEMMLCDACITPIYKSCPPHMYYRCDKCKFFLHLSCY-NLPSELEHDHGE--GLHKLSLGMKGAL-SWSDCDFCSRPTNGLFYRCEDCSYTIDVKCASVLAVIRHEANRQLKLIGTRVKQRSFFD--CSACGYSISYWYAYASEN-YDFNLHPECAMLPRIARHKLDKHPLSLAYPPYSHHSGDIYCEICEKEIHPKRWTYYCRECDQFFHAYCLTRSGWYRNIKFGHQLVLEKCHQSDHSLTFTL------GSLRHRCSLCPSVVYDEIAFECASCYFSCCSTCAG 608
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6UVN5 ((uncharacterized protein LOC113714884 {ECO:0000313|RefSeq:XP_027094814.1}))
HSP 1 Score: 256.914 bits (655), Expect = 1.239e-72
Identity = 186/543 (34.25%), Postives = 271/543 (49.91%), Query Frame = 0
HSP 2 Score: 90.8929 bits (224), Expect = 3.647e-16
Identity = 75/246 (30.49%), Postives = 111/246 (45.12%), Query Frame = 0
HSP 3 Score: 38.5058 bits (88), Expect = 5.624e+0
Identity = 42/173 (24.28%), Postives = 70/173 (40.46%), Query Frame = 0
Match: A0A6P6UVN5 ((uncharacterized protein LOC113714884 {ECO:0000313|RefSeq:XP_027094814.1}))
HSP 1 Score: 256.914 bits (655), Expect = 1.239e-72
Identity = 186/543 (34.25%), Postives = 271/543 (49.91%), Query Frame = 0
Query: 300 ICDVCSR--LVKFAYACEECTVDVCVFCG-------LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSS--DTVPIVTDTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQFATSNDD-----NDDNDDDRRVMICNGCIQPI-TDTHPSYYACVQCGFFLHSFCATKLPPEL--PRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCA---FLPKRIKHRSHKRHSLVQRPFSNSI--CSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGIC-EEGYNFLFFFECGGCGFLVCCNCF--GEITD 814
ICD C + F Y C C D+ V C LEQR + + H H L + + A F CDACG+ D+DSSY+C TC+FWIHK CAL P + + H+H L L YS+P HR+F ++C++C + V +W+YYC C YFVH+ C + S D + D E I E V LP + +LI K + IP+ +I SH H L L+ + D+ +DD ++ + +++C+ CI+PI + P YYAC +CG+ +H C+ LPP+L P+ HP H L + G+ C+ C TN YY C+ C++ I I+CA + +KH HK+H L Q S+ I + G +ACE C F + +C + P R+D H + L PP+F + FYC +CE+++N +YHC ECD+S H C+ KFG +N + H L V + A+ KS CG C ++ FEC C F +C +C E+TD
Sbjct: 94 ICDACGQDDWKFFTYHCSLCEFDLDVSCAILDQPEILEQREIELDCHH-HPLIQLEKPASFLCDACGKVDEDSSYLCPTCQFWIHKKCALRPTKVKHKD-HNHSLQLAYSLPSDHRKFPQQCSVCWKKVRLRDWVYYCGPCRYFVHITCVVISQKDEEQLSEDIEYP-ISGEQVQNVMKLPSNNTAQELIARFLLKEDEISNSNDSVKSNIPEEIFLI----SHRKHPLVLLEKVQNLDEIESTTSDDQEEAKALLVCDVCIEPICSSDDPHYYACAECGYVVHLTCSN-LPPKLHIPQ-----HPQHPFSLMHNPSAVGHFTCQACYRWTNANYYNCKPCELSICIKCASASMITSSVKHDGHKKHLLAQLQSSDRIRCTACGGYTYGGGFGFACEDC-HFYVGYNCALLPPTTTQRWDKHPLLLIYPPYFEHPEEFYCVLCEKEINPNHWMYHCRECDYSLHPLCIPQIGRVKFGRSLN--VNNHSHPLTYVPE--AKYKS---FCGSCNNRRLDWEEAFECDSCRFYLCPDCAYERELTD 615
HSP 2 Score: 90.8929 bits (224), Expect = 3.647e-16
Identity = 75/246 (30.49%), Postives = 111/246 (45.12%), Query Frame = 0
Query: 508 GHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGAS-SFHPSHSLVLYMSDQFYGY-VDCEVCGYCTNGFY-YKCEDCDIKIDIRCAFL--PKRIKHRSHK----RHSLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTL--REPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCL 742
H+SHE H L + +NDD DR+ ++C GC I D P+ Y C C FFLH CA ELPR H H LVL +Y C+ CG F+ Y C C+ +D+ CA L P+ ++ R + H L+Q S + K+ Y C TC F IH C + P+++ H+ H++ L P + C +C ++V + +Y+C C + H C+
Sbjct: 7 NHFSHEEHPL---ILSELRKENDDGSIDRKFVVCYGCQGQILD--PAAYCCFACHFFLHKRCA-----ELPRQIRHPMHSQHDLVLLGKPPYYSRSCICDACGQDDWKFFTYHCSLCEFDLDVSCAILDQPEILEQREIELDCHHHPLIQLEKPASFLCDACGKVDEDSSYLCPTC-QFWIHKKCALRPTKVKHKDHNHSLQLAYSLPSDHRKFPQQCSVCWKKVRLRDWVYYCGPCRYFVHITCV 241
HSP 3 Score: 38.5058 bits (88), Expect = 5.624e+0
Identity = 42/173 (24.28%), Postives = 70/173 (40.46%), Query Frame = 0
Query: 327 LEQRVLCHEGHQQHKLTL--MNRE--------ALFKCDAC-GEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVD 488
+E++ L H H++H L L + +E C C G+ ++Y C C F++HK CA P I + H L L+ P R C+ C + + + Y+C C + + + CA+ P + + ++D PL E + CGK D
Sbjct: 1 MEEKQLNHFSHEEHPLILSELRKENDDGSIDRKFVVCYGCQGQILDPAAYCCFACHFFLHKRCAELPRQIRHPMHSQHDLVLLGKPPYYSRSC--ICDACGQDDWKF-FTYHCSLCEFDLDVSCAILDQ--PEILEQREIELD----CHHHPLIQLEKPASFLCDACGKVDED 164
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7ADU0 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 256.529 bits (654), Expect = 1.413e-72
Identity = 191/559 (34.17%), Postives = 266/559 (47.58%), Query Frame = 0
HSP 2 Score: 66.2402 bits (160), Expect = 1.735e-8
Identity = 57/183 (31.15%), Postives = 82/183 (44.81%), Query Frame = 0
HSP 3 Score: 39.2762 bits (90), Expect = 4.193e+0
Identity = 53/225 (23.56%), Postives = 87/225 (38.67%), Query Frame = 0
Match: A0A5B7ADU0 (Phorbol-ester/DAG-type domain-containing protein {ECO:0000259|PROSITE:PS50081})
HSP 1 Score: 256.529 bits (654), Expect = 1.413e-72
Identity = 191/559 (34.17%), Postives = 266/559 (47.58%), Query Frame = 0
Query: 301 CDVC-SRLVKFAYACEECTVDVCVFCGLEQ--RVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCT-YFVHMRCAMSSDTVPIVTDTE---AADIDDETVLLQFP-LPHEESLFDLIITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQC-GFFLHSFCATKLPPELPRGAS-SFHPSHSLVLY-MSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAFLPKRIKHRSHKRHSL----------------------VQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYE---SFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVCCNC--FGEITDSTNSTS 820
C++C KF Y C C ++C+ C LE+ R + H+ HQ H L + R F CDAC E KD+SY+CT C FWIHK CA P I HHHPL L YS+P + +F+ C IC V W+YYC C YF H+ CA S ++ +E A D + L+ P LP + DLI KF + GE ES+T + H+SH++ + T D+ ++C+GC+ PI+ + P +Y+C QC FFLH C ELP+ S FH H L L+ Y C +C NGF Y C C+I +D++CA L IKH +H+ H + V++ FS S + K +G CETC + C + P + HRYD HA+ L PPF + FYCEICEE+++ +YHC +CD SFH C+ E +FKFG + PH L +V R+ C C + +++ FEC C +VC C +G+ S S
Sbjct: 81 CNLCRGSWNKFTYQCNNCEFNLCISCDLEEAERRIEHKSHQ-HPLDFLQRPVSFHCDACNTESKDTSYLCTACPFWIHKGCASLPSTIKIIG-HHHPLTLAYSLPVEYSKFKHTCQICSCKVKPTYWVYYCVGCRYYFAHVNCAKSKMEPERLSGSENEIKAKEDLDPNLIHLPALP--DKYVDLISHFPKKFNL---GENESATEVNQFSDDQLNHFSHDHPLILSGIQGTEEMKKDE-------ILCDGCVLPISLSAP-FYSCAQCTSFFLHKCCV-----ELPKRVSHPFHTEHPLFLHGKGPDISDYFTCGLCNIPCNGFSYNCAPCNIYLDVKCASLLGTIKHEAHESHPIQFKSDALPDISTAGLLSYFGQYVKKIFSPSTLCKACGKYFSGFSIRCETCDFSLCNDHCAMLPRTVRHRYDPHALYLSYPPFQDHPDEFYCEICEEEIDPNLWMYHCRDCDQSFHTKCIYKVEETLNFKFGETFK--ARNHPHPLTVV-----RENKRQSWCDRCSKYFDYKPAFECAPCNTIVCHQCILYGDARVSVEDIS 612
HSP 2 Score: 66.2402 bits (160), Expect = 1.735e-8
Identity = 57/183 (31.15%), Postives = 82/183 (44.81%), Query Frame = 0
Query: 563 FFLHSFCATKLPPELP-RGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCAF--LPKRIKHRSHKRH-SLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLRE--PPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHH 739
F LH CA ELP + HP H L+L++ Y DC +C N F Y+C +C+ + I C +RI+H+SH+ +QRP S C N + + Y C C F IH C PS + H +TL P + + C+IC +V +Y+C C + F H
Sbjct: 47 FHLHKMCA-----ELPEKTEHPMHPQHPLILHIDT---SYADCNLCRGSWNKFTYQCNNCEFNLCISCDLEEAERRIEHKSHQHPLDFLQRPVSFH-CDACNTE-SKDTSYLCTAC-PFWIHKGCASLPSTIKIIGHHHPLTLAYSLPVEYSKFKHTCQICSCKVKPTYWVYYCVGCRYYFAH 218
HSP 3 Score: 39.2762 bits (90), Expect = 4.193e+0
Identity = 53/225 (23.56%), Postives = 87/225 (38.67%), Query Frame = 0
Query: 588 HSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCED---CDIKIDIRCAFLPKRIKHRSHKRHSLVQRPFSN----SICSVSNLKITNGVEYACETCSSFQIHIDCVIYPS--RMNHRYDLHAVTLREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFF-FECGGCGF 802
H L+LY + + + + CE CG G YKC++ + CA LP++ +H H +H L+ ++ ++C S K T Y C C F + I C + + R+ H+ H + + P F+C+ C + + L C C H C + K G H L L + C IC ++ + C GC +
Sbjct: 10 HPLILYEAHEKF-HDRCEACGKGIIGPAYKCDEKFCFFFHLHKMCAELPEKTEHPMHPQHPLILHIDTSYADCNLCRGSWNKFT----YQCNNC-EFNLCISCDLEEAERRIEHKSHQHPLDFLQRPV----SFHCDACNTESKDTSYL--CTACPFWIHKGCASLPSTIKIIG--------HHHPLTLAYSLPVEYSKFKHTCQICSCKVKPTYWVYYCVGCRY 214
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A068V2T8 ((Uncharacterized protein {ECO:0000313|EMBL:CDP14976.1}))
HSP 1 Score: 250.366 bits (638), Expect = 3.411e-70
Identity = 179/526 (34.03%), Postives = 258/526 (49.05%), Query Frame = 0
HSP 2 Score: 89.3521 bits (220), Expect = 9.390e-16
Identity = 72/240 (30.00%), Postives = 105/240 (43.75%), Query Frame = 0
HSP 3 Score: 43.1282 bits (100), Expect = 2.106e-1
Identity = 49/195 (25.13%), Postives = 75/195 (38.46%), Query Frame = 0
HSP 4 Score: 38.1206 bits (87), Expect = 8.693e+0
Identity = 26/90 (28.89%), Postives = 43/90 (47.78%), Query Frame = 0
Match: A0A068V2T8 ((Uncharacterized protein {ECO:0000313|EMBL:CDP14976.1}))
HSP 1 Score: 250.366 bits (638), Expect = 3.411e-70
Identity = 179/526 (34.03%), Postives = 258/526 (49.05%), Query Frame = 0
Query: 300 ICDVCSR--LVKFAYACEECTVDVCVFCG-LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSS--DTVPIVTDTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQLEQLQF--ATSNDDNDDNDDDRRVMICNGCIQPI---TDTHPSYYACVQCGFFLHSFCATKLPPE--LPRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCA---FLPKRIKHRSHKRHSLVQRPFSNSI-CSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCL---RWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNFLFFFECGGCGFLVC 805
ICD C + F Y C C D+ V C LE ++ CH+ H L + + A F DACG+ D+DSSY+CT C FWIHK CAL P + + H+H L L YS+P HR F + C +C + V W+YYC C YFVH+ C + S D + D E I E LP + +LI K + IP+N ++ H H E++Q + +DD ++ + +++C+ CI+PI D H YYACV+CG+F+H C + LPPE LP+ HP H L G CE C + TN +Y CE C++ I+CA + ++H HK+H L Q S+ I C+ + CE C F + +C + P R+D H + L PP++ + VFYC +CE ++N +YHC ECD+S H +C+ + KFG +N + H L V + A+ KS CG C ++ FEC C F +C
Sbjct: 99 ICDACGQGGWKFFTYHCSRCEFDLDVSCAILEIKLHCHD----HPLRQLKKPAFFLGDACGKLDEDSSYLCTVCPFWIHKKCALRPTSVKHKD-HNHLLRLAYSLPSEHRSFRQYCPVCRDKVQLSRWVYYCGPCRYFVHVACVVISQEDEGQLSEDIEYP-ISGEQDQNVVKLPSNNAAQELIARFLLKEDEISSSNDSDKSNIPENI-FMDSHRKHPLVLSEKVQNLDEIKSTTSDDQEEAKALLVCDVCIEPICSSDDLH--YYACVKCGYFVHLTC-SNLPPELHLPK-----HPQHPFSLTCKSSAVGGFICEACPWWTNASFYNCEPCELSFCIKCASASMITSSVRHDGHKKHLLTQLQSSDLIRCTACGCVRKGSFAFVCEDC-HFYVCFNCALLPPATTQRWDKHPLLLIYPPYYEHPEVFYCVLCEIEINPNHWMYHCRECDYSLHPWCIPQVNLFRRVKFGRSLN--VNNHSHPLTHVPE--AKYKS---FCGSCNWRLDWEEAFECDSCRFDLC 601
HSP 2 Score: 89.3521 bits (220), Expect = 9.390e-16
Identity = 72/240 (30.00%), Postives = 105/240 (43.75%), Query Frame = 0
Query: 509 HWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGASSFHPSHSLVLYMSDQF-YGYVDCEVCGYCTNGFY-YKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLVQRP--FSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTL--REPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCL 742
H+SHE H L + +NDD D++ +C GC + I D P+ Y C C F LH CA KLP ++ H H LVL + G C+ CG F+ Y C C+ +D+ CA L ++ H L ++P F C K+ Y C C F IH C + P+ + H+ H + L P YC +C ++V R +Y+C C + H C+
Sbjct: 13 HFSHEEHPLILCEL---QKENDDGSIDQKSAVCYGCQEQILD--PAAYCCFACNFLLHKRCA-KLPRQI---THPMHSQHPLVLLGKPPYSSGSCICDACGQGGWKFFTYHCSRCEFDLDVSCAILEIKLHCHDHPLRQL-KKPAFFLGDACG----KLDEDSSYLCTVC-PFWIHKKCALRPTSVKHKDHNHLLRLAYSLPSEHRSFRQYCPVCRDKVQLSRWVYYCGPCRYFVHVACV 237
HSP 3 Score: 43.1282 bits (100), Expect = 2.106e-1
Identity = 49/195 (25.13%), Postives = 75/195 (38.46%), Query Frame = 0
Query: 616 YKCEDCDIKIDIRCAFLPKRIKHRSHKRHSLV---QRPFSNS--ICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGICEEGYNF-LFFFECGGCGFLV 804
Y C C+ + RCA LP++I H H +H LV + P+S+ IC Y C C F + + C I ++ H +D L++P FF C +E + Y C C H C S K ++ H L L + +S C +C + + + CG C + V
Sbjct: 55 YCCFACNFLLHKRCAKLPRQITHPMHSQHPLVLLGKPPYSSGSCICDACGQGGWKFFTYHCSRC-EFDLDVSCAILEIKL-HCHDHPLRQLKKPAFFLGDA--CGKLDEDSS-----YLCTVCPFWIHKKCALRPTSVKH--------KDHNHLLRLAYSLPSEHRSFRQYCPVCRDKVQLSRWVYYCGPCRYFV 232
HSP 4 Score: 38.1206 bits (87), Expect = 8.693e+0
Identity = 26/90 (28.89%), Postives = 43/90 (47.78%), Query Frame = 0
Query: 353 CDACGEEDKD-SSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAM 441
C C E+ D ++Y C C F +HK CA P I + HPL L+ P + C+ C + + + Y+C +C + + + CA+
Sbjct: 42 CYGCQEQILDPAAYCCFACNFLLHKRCAKLPRQITHPMHSQHPLVLLGKPP--YSSGSCICDACGQGGWKF-FTYHCSRCEFDLDVSCAI 128
BLAST of DCAR_025679 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6TS23 ((uncharacterized protein LOC113703925 isoform X1 {ECO:0000313|RefSeq:XP_027081249.1}))
HSP 1 Score: 249.595 bits (636), Expect = 5.605e-70
Identity = 182/542 (33.58%), Postives = 264/542 (48.71%), Query Frame = 0
HSP 2 Score: 90.5077 bits (223), Expect = 4.521e-16
Identity = 75/247 (30.36%), Postives = 109/247 (44.13%), Query Frame = 0
HSP 3 Score: 38.891 bits (89), Expect = 5.030e+0
Identity = 43/174 (24.71%), Postives = 72/174 (41.38%), Query Frame = 0
Match: A0A6P6TS23 ((uncharacterized protein LOC113703925 isoform X1 {ECO:0000313|RefSeq:XP_027081249.1}))
HSP 1 Score: 249.595 bits (636), Expect = 5.605e-70
Identity = 182/542 (33.58%), Postives = 264/542 (48.71%), Query Frame = 0
Query: 300 ICDVCSR--LVKFAYACEECTVDVCVFCG-------LEQRVLCHEGHQQHKLTLMNREALFKCDACGEEDKDSSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSS--DTVPIVTDTEAADIDDETVLLQFPLPHEESLFDLIITQCGKFQVDLQGEGESSTPIPDNPHIIEGHWSHENHQL------EQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHP-SYYACVQCGFFLHSFCATKLPPEL--PRGASSFHPSHSLVLYMSDQFYGYVDCEVCGYCTNGFYYKCEDCDIKIDIRCA---FLPKRIKHRSHKRHSLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLREPPFF-YEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCLRWYESFKFGGIINRIIQEQPHALALVLKRTARDKSPLYICGIC-EEGYNFLFFFECGGCGFLVCCNCF--GEITD 814
ICD C + F Y C C D+ V C LE R + + H H+L + + A F CDACG+ D+DSSY+CT C FWIHK CAL P + + H+HPL L YS+P +R+F ++C++C + V +W+YYC C YFVH+ C + S D + D E I E LP + +LI + + IP+ +I SH H L + L S +D + +++C+ CI+PI + YYAC +CG+F+H C+ LPPEL P+ HP H L G C C + TN Y C+ C++ I I+CA + +KH HK+H L Q S+ I + ++ G +ACE C F + DC + P R+D H + L PP+F + FYC +CE+++N +YHC ECD+S H C+ K+G +N + H L V + A+ KS CG C ++ FEC C F +C +C E+TD
Sbjct: 94 ICDACGQDDWKFFTYHCSLCEFDLDVSCAILDQPEILEPREIELDCHH-HRLIQLEKPASFLCDACGKVDEDSSYLCTICPFWIHKKCALRPTTVKHKD-HNHPLHLAYSLPSDYRKFSQQCSVCWKKVRLRDWVYYCGPCRYFVHITCVVISQKDEEQLSEDNEYP-ISGEQDQNVVKLPSSNAAQELIARFLLREDEMSSSNDSDKSNIPEKIFLI----SHRKHPLVLSEKVQNLDEIKSTTSDDQEEAKALLLVCDVCIEPICSSDDLQYYACAECGYFVHLTCSN-LPPELHIPK-----HPQHPFSLTCKSSEVGRFICGACHWWTNAACYNCKPCELSICIKCASASMITSSVKHDGHKKHLLTQFHSSDPIHCTACDFLSGGFRFACEDC-HFYVCYDCALLPPTTTQRWDKHPLLLIYPPYFEHPEEFYCVLCEKEINPNHWMYHCRECDYSLHPLCIPQIGRVKYGRFLN--VNNHSHPLTHVPE--AKYKS---FCGSCNNRRLDWEEAFECDSCRFYLCPDCAYERELTD 614
HSP 2 Score: 90.5077 bits (223), Expect = 4.521e-16
Identity = 75/247 (30.36%), Postives = 109/247 (44.13%), Query Frame = 0
Query: 508 GHWSHENHQLEQLQFATSNDDNDDNDDDRRVMICNGCIQPITDTHPSYYACVQCGFFLHSFCATKLPPELPRGAS-SFHPSHSLVLYMSDQFYGY-VDCEVCGYCTNGFY-YKCEDCDIKIDIRCAFL-------PKRIKHRSHKRHSLVQRPFSNSICSVSNLKITNGVEYACETCSSFQIHIDCVIYPSRMNHRYDLHAVTLRE--PPFFYEGVFYCEICEEQVNNQRLLYHCDECDHSFHHYCL 742
H+SHE H L + +NDD DR+ ++C GC + I D P+ Y C C FFLH CA ELPR H H LVL +Y C+ CG F+ Y C C+ +D+ CA L P+ I+ H H L+Q S + K+ Y C C F IH C + P+ + H+ H + L P + + C +C ++V + +Y+C C + H C+
Sbjct: 7 NHFSHEEHPL---ILSELRKENDDGSIDRKFVVCYGCQEQILD--PAAYCCFACHFFLHKRCA-----ELPRQIRHPMHSQHDLVLLGKPPYYSRSCICDACGQDDWKFFTYHCSLCEFDLDVSCAILDQPEILEPREIELDCH-HHRLIQLEKPASFLCDACGKVDEDSSYLCTIC-PFWIHKKCALRPTTVKHKDHNHPLHLAYSLPSDYRKFSQQCSVCWKKVRLRDWVYYCGPCRYFVHITCV 241
HSP 3 Score: 38.891 bits (89), Expect = 5.030e+0
Identity = 43/174 (24.71%), Postives = 72/174 (41.38%), Query Frame = 0
Query: 327 LEQRVLCHEGHQQHKLTL--MNRE--------ALFKCDACGEEDKD-SSYVCTTCEFWIHKTCALSPYIIPTQAYHHHPLFLIYSIPQMHRQFERECNICHEVVYNINWMYYCHKCTYFVHMRCAMSSDTVPIVTDTEAADID-DETVLLQFPLPHEESLFDLIITQCGKFQVD 488
+E++ L H H++H L L + +E C C E+ D ++Y C C F++HK CA P I + H L L+ P R C+ C + + + Y+C C + + + CA+ P + + ++D L+Q P + CGK D
Sbjct: 1 MEEKQLNHFSHEEHPLILSELRKENDDGSIDRKFVVCYGCQEQILDPAAYCCFACHFFLHKRCAELPRQIRHPMHSQHDLVLLGKPPYYSRSC--ICDACGQDDWKF-FTYHCSLCEFDLDVSCAILDQ--PEILEPREIELDCHHHRLIQLEKP-----ASFLCDACGKVDED 164
| Match Name | Stats | Description |
|---|---|---|
| A0A5B7ABQ0 | E-Value: 1.609e-78, PID: 34.66 | Phorbol-ester/DAG-type domain-containing protein {... [more] |
| A0A5B7ABP9 | E-Value: 4.307e-78, PID: 34.90 | Phorbol-ester/DAG-type domain-containing protein {... [more] |
| A0A6P6WHB0 | E-Value: 2.915e-76, PID: 33.33 | (uncharacterized protein LOC113732809 {ECO:0000313... [more] |
| A0A6P6VYH3 | E-Value: 1.017e-75, PID: 33.64 | (uncharacterized protein LOC113727606 {ECO:0000313... [more] |
| A0A068V412 | E-Value: 1.532e-75, PID: 33.64 | Phorbol-ester/DAG-type domain-containing protein {... [more] |
| A0A2G9HES0 | E-Value: 8.448e-73, PID: 33.15 | (Uncharacterized protein {ECO:0000313|EMBL:PIN1600... [more] |
| A0A6P6UVN5 | E-Value: 1.239e-72, PID: 34.25 | (uncharacterized protein LOC113714884 {ECO:0000313... [more] |
| A0A5B7ADU0 | E-Value: 1.413e-72, PID: 34.17 | Phorbol-ester/DAG-type domain-containing protein {... [more] |
| A0A068V2T8 | E-Value: 3.411e-70, PID: 34.03 | (Uncharacterized protein {ECO:0000313|EMBL:CDP1497... [more] |
| A0A6P6TS23 | E-Value: 5.605e-70, PID: 33.58 | (uncharacterized protein LOC113703925 isoform X1 {... [more] |
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back to topAnalysis:
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |