MEEARRRQMCLSYVLLLAISSLACVLASDADTIFYEPFDEEFSGRWIVSE
KDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQ
FETRLQNGLECGDATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDP
EASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWH
APLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILF
DNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKK
VFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVL
LKLLFGGKKPTKVTAEVKKTDAAEPSSIQESDEKKDDSDDTAAPRRRTVR
RDD*
| Relationships |
|---|
| The polypeptide, DCAR_016330, derives from mRNA, DCAR_016330. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A446W452 ((Uncharacterized protein {ECO:0000313|EMBL:VAI54890.1}))
HSP 1 Score: 504.597 bits (1298), Expect = 4.987e-176
Identity = 285/432 (65.97%), Postives = 323/432 (74.77%), Query Frame = 0
Query: 14 VLLLAISSL-ACVLASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA-----------------------------------------TKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKK---PTKVTAEVKKTDAAEPSSIQESDEKKDDSDDTAAPRRRTVR 400
VLLL +S+L A + ASD +F+E FDE F G WIVS K+EY G WKH KS+GHEDYGLLVSEPARKYAIVKEL+ PV LKDGTVVLQFE RLQNGLECG A KPDDWDEDAP EI DEEATKPEGWLDDEPEE++DPEA+KPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWK+PMK NPAYKGKWHAP+IDNP+YKGIWKPQ+IPNP YFELD+PD++PIAAIGIEIWTMQDGILFDNILIA DEKVA + + WKPKFDVEKEKQKAEE A++ S+ L QKK+F +LYKIADIPFL+ +K KI+D IEK EKQPN+TI +L S V+ +VL + LFGGKK P K AEVKK A E + S +K++ DDT APRRR+ R
Sbjct: 7 VLLLVVSALFAQIYASDP--LFHESFDEGFQGSWIVSGKEEYSGVWKHEKSDGHEDYGLLVSEPARKYAIVKELDSPVTLKDGTVVLQFEVRLQNGLECGGAYLKYIRPQEAGWDAKEFDNDTPYTIMFGPDKCGSTNKVHFILKPDDWDEDAPTEILDEEATKPEGWLDDEPEEVDDPEAAKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKRPMKQNPAYKGKWHAPMIDNPAYKGIWKPQEIPNPEYFELDKPDFDPIAAIGIEIWTMQDGILFDNILIADDEKVATAILEKTWKPKFDVEKEKQKAEEAAAADSEDLSEFQKKIFSVLYKIADIPFLEPYKIKIIDVIEKGEKQPNITISILASAAVILVTVLFRTLFGGKKPVAPVKPIAEVKKPSATEADAAGSSGDKEEKEDDTTAPRRRSRR 436
Match: A0A446V5X5 ((Uncharacterized protein {ECO:0000313|EMBL:VAI43288.1}))
HSP 1 Score: 481.485 bits (1238), Expect = 6.171e-167
Identity = 275/415 (66.27%), Postives = 314/415 (75.66%), Query Frame = 0
Query: 30 ADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA-----------------------------------------TKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKK---PTKVTAEVKKTDAAEPSSIQESDEKKDDSDDTAAPRRRTVR 400
+D +F+E FDE F G W+VS K+EY G WKH KS+GHEDYGLLVSEPARKYAIVKEL+ PV LKDGTVVLQFE RLQNGLECG A KPDDWDEDAP EI DEEATKPEGWLDDEPEE++DPEA+KPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWK+PMK NPAYKGKWHAPLIDNP+YKGIWKPQ+IPNP YFELD+PD++PIAAIGIEIWTMQDGILFDNILIA DEKVA + + WKPKFDVEKEKQKAEE A++ S+ L QKK+F +LYKIADIPFL+ +K KI+D IEK EKQPN+TI +L S+ V+ +VL + +FGGKK P K AEVKK +A E + S +K++ DDT APRRR+ R
Sbjct: 26 SDPLFHESFDEGFEGSWVVSGKEEYSGVWKHEKSDGHEDYGLLVSEPARKYAIVKELDSPVTLKDGTVVLQFEVRLQNGLECGGAYLKYIRPQEAGWDAKEFDNDTPYTIMFGPDKCGSTNKVHFILKPDDWDEDAPTEILDEEATKPEGWLDDEPEEVDDPEAAKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKRPMKQNPAYKGKWHAPLIDNPAYKGIWKPQEIPNPEYFELDKPDFDPIAAIGIEIWTMQDGILFDNILIADDEKVATAILEKTWKPKFDVEKEKQKAEEAAAADSEDLSEFQKKIFSVLYKIADIPFLEPYKIKIIDIIEKGEKQPNITISILASVAVILVTVLFRTIFGGKKPVAPVKPVAEVKKPNATEADAAGSSGDKEEKEDDTTAPRRRSRR 440
Match: A0A2N9FN82 ((Uncharacterized protein {ECO:0000313|EMBL:SPC88540.1}))
HSP 1 Score: 461.07 bits (1185), Expect = 5.431e-158
Identity = 265/494 (53.64%), Postives = 312/494 (63.16%), Query Frame = 0
Query: 11 LSYVLLLAISSLACVLASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDAT-----------KPDDWDEDAPMEIE--------------------------------DEEATKPEGWL---DDEP-----EEIEDPEASKPEDWDD---------------EEDGEWE----------------------APKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTKVTAEVKKTDAAEPSSIQESD-------------EKKDDSDDTAAPRRRTVRRDD 403
L V LL + + ASD TIFYE FDE F GRWIVS+K+EYQG WKHSKSEGH+DYGLLVSE ARKYAIVKE + PV+LKDGT+VLQFETRLQ GLECG A KP ++D D+P I D E K +L D EP + I DP+ KPEDWD+ +ED E APK DNPKC+ APGCGEWKKPMKSNPAYKGKW+APLIDNP YKGIWKPQQIPNP YFEL+RPD+EPIAAIGIEIWTMQDGILFDN+LIA DEKVAESYR+T WKPKF+VEKEKQKAE+ A + SD + QKKVF++LY+IAD+PFL +K +ILD +EKAEKQPNLTIG+L SI VV F+V+ +++FGGKK +V E T AE S+ Q S EK+ + ++T PRRR RR++
Sbjct: 12 LLLVTLLFFIAFQELSASDDQTIFYESFDETFEGRWIVSDKEEYQGVWKHSKSEGHDDYGLLVSEKARKYAIVKETDSPVSLKDGTIVLQFETRLQEGLECGGAYLKYLRPQEAGWKPKEFDNDSPYTIMFGPDKCGATNKDKLTHVYTAVLKPDNELRILVDGEEKKKANFLSTEDFEPALIPAKTIPDPDDKKPEDWDEREKIPEPNAVKPEDWDEDAPMEIEDEEARTKPEDWDDEEDGEWEAPKTDNPKCDTAPGCGEWKKPMKSNPAYKGKWNAPLIDNPDYKGIWKPQQIPNPDYFELERPDFEPIAAIGIEIWTMQDGILFDNVLIAKDEKVAESYRETTWKPKFEVEKEKQKAEDVA-TGSDSVTSFQKKVFDILYQIADVPFLSTYKPQILDLLEKAEKQPNLTIGILASIAVVIFTVIFRIIFGGKKNVRVEKE--NTRVAETSNNQGSSGDKEEEKEKEKEKEKEKEEEETVPPRRRNARREN 502
Match: A0A7J7NNB4 ((Uncharacterized protein {ECO:0000313|EMBL:KAF6168717.1}))
HSP 1 Score: 441.039 bits (1133), Expect = 1.478e-149
Identity = 215/296 (72.64%), Postives = 250/296 (84.46%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPT-KVTAEVKKTDAAEPSSIQE---SDEKKDDSDD-TAAPRRRTVRRDD 403
+A+KPDDWDEDAPME+ED EA KPEGWLDDEPEEI+DPEASKPEDWDDEEDG WE P +DNPKCE APGCGEWKKPMK NPAYKGKWHA LIDNPSYKGIWKPQQI NP YFEL++PD+EP+AAIGIEIWTMQDGILFDNILIA+DEKVAESYR+ WKPKF+VEKEKQK+E+EA+ SDGL G QKKVF+LLYK+AD+PFL +K +ILD IEK EKQPN+TIGVLVS+ VV S++ K+LFGGKKP KV E + ++PS + S K++++DD AAPRRR RR++
Sbjct: 242 EASKPDDWDEDAPMEVEDAEAEKPEGWLDDEPEEIDDPEASKPEDWDDEEDGAWEPPMVDNPKCEAAPGCGEWKKPMKQNPAYKGKWHASLIDNPSYKGIWKPQQIDNPDYFELEKPDFEPVAAIGIEIWTMQDGILFDNILIAADEKVAESYREKTWKPKFEVEKEKQKSEDEAAGISDGLAGFQKKVFDLLYKVADVPFLAAYKLQILDIIEKGEKQPNITIGVLVSVAVVILSIIYKILFGGKKPVAKVVTEEESKSTSKPSDTTDQGTSGAKEEETDDAAAAPRRRNTRREN 537
HSP 2 Score: 166.392 bits (420), Expect = 1.099e-43
Identity = 81/112 (72.32%), Postives = 91/112 (81.25%), Query Frame = 0
Query: 3 EARRRQMCLSYVLLLAISSLACVLASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA 114
E +R M L +L+ + C+ +D+ FYE FDE F GRWIVSEKDEYQG+WKHSKSEGH+DYGLLVSEPARKYAIVKELEEPV+LKDGTVVLQFETRLQNGLECG A
Sbjct: 2 EEKRISMQLILLLIACFTFQLCL----SDSTFYESFDEPFEGRWIVSEKDEYQGSWKHSKSEGHDDYGLLVSEPARKYAIVKELEEPVSLKDGTVVLQFETRLQNGLECGGA 109
Match: A0A5B7ALH4 ((Uncharacterized protein {ECO:0000313|EMBL:MPA57560.1}))
HSP 1 Score: 436.032 bits (1120), Expect = 1.573e-149
Identity = 224/296 (75.68%), Postives = 258/296 (87.16%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTK-VTAEVKKTDAAEP----SSIQESDEKKDDSDDTAAPRRRTVRRDD 403
DA KPDDWDEDAPMEIEDEEA KPEGWLDDEPEEI+DPEA+KPEDWDDEEDG WEAPKIDNPKCE APGCGEWKKP+K NPAYKGKWH+PLIDNP+YKGIWKPQQIPNP YFELD+PD+EPIAAIGIEIWTMQDG+LFDNILIASDEKVAE+YR+T WKPKF+VEKEKQKAE+EA+ SDGLKG QK VF++LYKIAD+PFL +K KILD IEK EKQPNLTIG++VS++VV +VLLK++FGGKKP K VTAE ++ + + S +E +E+ D D TA PRRR +RR++
Sbjct: 108 DAVKPDDWDEDAPMEIEDEEAVKPEGWLDDEPEEIDDPEATKPEDWDDEEDGLWEAPKIDNPKCEAAPGCGEWKKPVKRNPAYKGKWHSPLIDNPNYKGIWKPQQIPNPNYFELDKPDFEPIAAIGIEIWTMQDGVLFDNILIASDEKVAETYRETAWKPKFEVEKEKQKAEDEAAGVSDGLKGFQKAVFDILYKIADLPFLSAYKIKILDVIEKGEKQPNLTIGIIVSVIVVILTVLLKIIFGGKKPEKVVTAETEEAETSNNQGSGSGGEEKEEQSDKEDATAPPRRRNIRREN 403
Match: A0A6J0LBV7 ((calnexin homolog 1 {ECO:0000313|RefSeq:XP_018457124.1}))
HSP 1 Score: 439.499 bits (1129), Expect = 4.535e-149
Identity = 218/293 (74.40%), Postives = 249/293 (84.98%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKK--PTKVTAEVKKTDAAEPSSIQESDEKKDDSDDTAAPRRRTVRRDD 403
+A KP+DWDE+APMEIEDEEA KPEGWLDDEPEE++DPEA+KPEDWDDEEDG WEAPKIDNPKCE APGCGEWK+PMK NPAYKGKW AP+IDNP+YKGIWKP+ IPNP YFELDRPDYEPIAAIGIEIWTMQDGILFDNILIA DEKVAESYRQT WKPKFDVEKEKQ+AEE+A+ ++DGLK QK VF+LL K+AD+ FL +K KI + IEKAE+QPNLTIGVLVSIVVVFFS+ +KL+FGGKK P + KK + E S + EKK ++TAAPR+R RRD+
Sbjct: 246 NAVKPEDWDEEAPMEIEDEEAEKPEGWLDDEPEEVDDPEATKPEDWDDEEDGMWEAPKIDNPKCESAPGCGEWKRPMKKNPAYKGKWSAPMIDNPAYKGIWKPRDIPNPDYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIAKDEKVAESYRQTTWKPKFDVEKEKQQAEEKAAESADGLKSYQKVVFDLLNKVADLSFLSAYKSKITELIEKAEEQPNLTIGVLVSIVVVFFSLFIKLIFGGKKVAPAATVEKKKKPEVGESSKSGDESEKK---EETAAPRKRQPRRDN 535
HSP 2 Score: 142.124 bits (357), Expect = 8.364e-35
Identity = 67/111 (60.36%), Postives = 82/111 (73.87%), Query Frame = 0
Query: 5 RRRQMCLSYVLLLAISSLACVLASDAD-TIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA 114
R RQ+ + +L+L SL + D D T+ YE FDE F GRWIVS+ +Y+G WKH KSEGH+DYGLLVSE ARKY IVK L+EP+NLK+GT+VLQ+E R Q GLECG A
Sbjct: 3 RDRQLFSAVLLILVFVSLQKLCYCDDDQTVLYESFDEAFDGRWIVSKNGDYEGVWKHEKSEGHDDYGLLVSEKARKYGIVKVLDEPLNLKEGTIVLQYEVRFQEGLECGGA 113
Match: A0A200R3N5 ((Calreticulin/calnexin {ECO:0000313|EMBL:OVA17329.1}))
HSP 1 Score: 442.58 bits (1137), Expect = 5.893e-149
Identity = 213/269 (79.18%), Postives = 238/269 (88.48%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTKVTAEVKKTDAAEPSSIQES 381
DA KP+DWDEDAPMEIEDEEA KPEGWLDDEPEEI+DPEASKPEDWD++EDGEWEAPKIDNPKCE APGCGEWKKPMK NPAYKGKWHAPLIDNP+YKGIWKPQ+IPNP YF+LD+PD+EP+AAIGIEIWTMQDGILFDNILIASDEKVAESYR+T WKPKF+VEKEKQKAEE A+ SDGL G QKKVF+LLYK+ DIPFL +K KI D IEK EKQPNLTIG+LVSI+VV +V ++LFGGKKPT T E K+T +E ++ Q S
Sbjct: 340 DAVKPEDWDEDAPMEIEDEEAEKPEGWLDDEPEEIDDPEASKPEDWDNDEDGEWEAPKIDNPKCEAAPGCGEWKKPMKRNPAYKGKWHAPLIDNPNYKGIWKPQEIPNPNYFDLDKPDFEPVAAIGIEIWTMQDGILFDNILIASDEKVAESYRETTWKPKFEVEKEKQKAEEAAAGLSDGLSGFQKKVFDLLYKVGDIPFLDAYKLKIFDLIEKGEKQPNLTIGILVSIIVVILTVFYRILFGGKKPT-ATKERKETGTSETANNQGS 607
HSP 2 Score: 154.836 bits (390), Expect = 5.645e-39
Identity = 78/119 (65.55%), Postives = 90/119 (75.63%), Query Frame = 0
Query: 23 ACV--LASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDAT-----------KPDDWDEDAPMEI 128
ACV L+ +DTIFY+ FD+ F G WIVSEKDEY G WKHSKSEGH+DYGLLVSE ARKYAIVKEL EPV+LKDGT+VLQFETRLQNGLECG A KP +++ ++P I
Sbjct: 114 ACVTFLSCASDTIFYDAFDKSFEGSWIVSEKDEYNGVWKHSKSEGHDDYGLLVSEKARKYAIVKELNEPVSLKDGTIVLQFETRLQNGLECGGAYLKYLRPQEAGWKPKEFNNESPYSI 232
Match: A0A5B7C3F8 ((Putative Calnexin-1-like protein {ECO:0000313|EMBL:MPA75699.1}))
HSP 1 Score: 434.106 bits (1115), Expect = 7.488e-149
Identity = 227/296 (76.69%), Postives = 259/296 (87.50%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTKVTAEVKKT--DAAEPSSIQESDEKKDDSDDTAA---PRRRTVRRDD 403
DA KPDDWDEDAPMEIEDEEA KPEGWLDDEPEEI+DPEA+KPEDWDDEEDG WEAPKIDNPKCE APGCGEWKKP+K NPAYKGKWHAPLIDNP+YKGIWKPQ+IPNP YFEL++PD+EP+AAIGIEIWTMQDGILFDN LIAS+EKVAE+YR+T WKPKF+VEKEKQKAE+EA+ SDGLKG QK VF+LLYKIADIPFL +K ILD IEK EKQPNLTIG++VSIVVV F+VLLK++FGGKKP +V E + T AAE S+ Q S E+K+D +D++A P RRT RR++
Sbjct: 108 DAVKPDDWDEDAPMEIEDEEAVKPEGWLDDEPEEIDDPEATKPEDWDDEEDGLWEAPKIDNPKCEAAPGCGEWKKPVKRNPAYKGKWHAPLIDNPNYKGIWKPQEIPNPNYFELEKPDFEPLAAIGIEIWTMQDGILFDNFLIASNEKVAETYRETTWKPKFEVEKEKQKAEDEAAGISDGLKGYQKVVFDLLYKIADIPFLSAYKHNILDLIEKGEKQPNLTIGIIVSIVVVIFTVLLKIIFGGKKPARVAVETQNTAEAAAETSNNQGSGEEKEDKEDSSASAVPHRRTARREN 403
Match: A0A5B7C4N9 ((Putative Calnexin-1-like protein {ECO:0000313|EMBL:MPA75855.1}))
HSP 1 Score: 432.95 bits (1112), Expect = 2.483e-146
Identity = 225/296 (76.01%), Postives = 258/296 (87.16%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTKVTAEVKKT--DAAEPSSIQESDEKKDDSDDTAA---PRRRTVRRDD 403
DA KPDDWDEDAPMEIEDEEA KPEGWLDDEPEEI+DPEA+KPEDWDDEEDG WEAPKIDNPKCE +PGCGEWK+P K NPAYKGKWHAPLIDNP+YKGIWKPQ+IPNP YFEL++PD+EP+AAIGIEIWTMQDGILFDN LIAS+EKVAE+YR+T WKPKF+VEKEKQKAE+EA+ SDGLKG QK VF+LLYKIADIPFL +K ILD IEK EKQPNLTIG++VSIVVV F+VLLK++FGGKKP +V E + T AAE S+ Q S E+K+D +D++A P RRT RR++
Sbjct: 247 DAVKPDDWDEDAPMEIEDEEAVKPEGWLDDEPEEIDDPEATKPEDWDDEEDGLWEAPKIDNPKCETSPGCGEWKRPTKRNPAYKGKWHAPLIDNPNYKGIWKPQEIPNPNYFELEKPDFEPLAAIGIEIWTMQDGILFDNFLIASNEKVAETYRETTWKPKFEVEKEKQKAEDEAAGISDGLKGYQKVVFDLLYKIADIPFLSAYKHNILDLIEKGEKQPNLTIGIIVSIVVVIFTVLLKIIFGGKKPARVAVETQNTAEAAAETSNNQGSGEEKEDKEDSSASAVPHRRTARREN 542
HSP 2 Score: 175.637 bits (444), Expect = 4.464e-47
Identity = 81/114 (71.05%), Postives = 94/114 (82.46%), Query Frame = 0
Query: 1 MEEARRRQMCLSYVLLLAISSLACVLASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA 114
MEE +RR++ L +VLLL I A L + +D IFY+ FDE F GRWIVSEKD+Y+G WKH+KSEGH+DYGLLVSE ARKYAIVKEL+EPVNLKDGT+VLQFE R QNGLECG A
Sbjct: 1 MEEGKRRKIYLQFVLLLLIGCFASQLCASSDPIFYDSFDESFEGRWIVSEKDDYKGVWKHAKSEGHDDYGLLVSEAARKYAIVKELDEPVNLKDGTIVLQFEDRFQNGLECGGA 114
Match: A0A565CV96 ((Uncharacterized protein {ECO:0000313|EMBL:VVB17625.1}))
HSP 1 Score: 431.024 bits (1107), Expect = 7.826e-146
Identity = 215/291 (73.88%), Postives = 237/291 (81.44%), Query Frame = 0
Query: 113 DATKPDDWDEDAPMEIEDEEATKPEGWLDDEPEEIEDPEASKPEDWDDEEDGEWEAPKIDNPKCEEAPGCGEWKKPMKSNPAYKGKWHAPLIDNPSYKGIWKPQQIPNPIYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIASDEKVAESYRQTKWKPKFDVEKEKQKAEEEASSASDGLKGIQKKVFELLYKIADIPFLQNHKFKILDAIEKAEKQPNLTIGVLVSIVVVFFSVLLKLLFGGKKPTKVTAEVKKTDAAEPSSIQESDEKKDDSDDTAAPRRRTVRRDD 403
+A+KPDDWDEDAPMEIEDEEA KPEGWLDDEPEE++DPEA+KPEDWDDEEDG WEAPKIDNPKCE APGCGEWK+PMK NPAYKGKW APLIDNP+YKGIWKP+ IPNP YFELDRPD+EPIAAIGIEIWTMQDGILFDNILIA DEKVAE+YRQT WKPKFDVEKEKQKAEE A+ +SDGLK QK VF+LL K+ADI FL +K KI + IEKAE+QPNLTIGVLVSIVVVFFS+ LKL+FGGK K A V AAPR+R RR++
Sbjct: 244 NASKPDDWDEDAPMEIEDEEAEKPEGWLDDEPEEVDDPEATKPEDWDDEEDGMWEAPKIDNPKCETAPGCGEWKRPMKRNPAYKGKWSAPLIDNPAYKGIWKPRDIPNPDYFELDRPDFEPIAAIGIEIWTMQDGILFDNILIAKDEKVAETYRQTTWKPKFDVEKEKQKAEEAAAGSSDGLKSYQKIVFDLLNKVADISFLSAYKSKITELIEKAEQQPNLTIGVLVSIVVVFFSLFLKLIFGGK---KAAATVXXXXXXXXXXXXXXXXXXXXXXTAAAPRKRQPRREN 531
HSP 2 Score: 142.124 bits (357), Expect = 7.237e-35
Identity = 70/110 (63.64%), Postives = 82/110 (74.55%), Query Frame = 0
Query: 5 RRRQMCLSYVLLLAISSLACVLASDADTIFYEPFDEEFSGRWIVSEKDEYQGAWKHSKSEGHEDYGLLVSEPARKYAIVKELEEPVNLKDGTVVLQFETRLQNGLECGDA 114
R RQ+ +LLLA S + D T+ YE FDE F GRWIVS+ +Y+G WKHSKSEGH+DYGLLVSE ARKY IVKEL+EP+NLK+GTVVLQ+E R Q GLECG A
Sbjct: 2 RERQLFSGLLLLLAFVSFQKLCYCDDQTVLYESFDEPFDGRWIVSKNSDYEGVWKHSKSEGHDDYGLLVSEKARKYGIVKELDEPLNLKEGTVVLQYEVRFQEGLECGGA 111
| Match Name | Stats | Description |
|---|---|---|
| A0A446W452 | E-Value: 4.987e-176, PID: 65.97 | (Uncharacterized protein {ECO:0000313|EMBL:VAI5489... [more] |
| A0A446V5X5 | E-Value: 6.171e-167, PID: 66.27 | (Uncharacterized protein {ECO:0000313|EMBL:VAI4328... [more] |
| A0A2N9FN82 | E-Value: 5.431e-158, PID: 53.64 | (Uncharacterized protein {ECO:0000313|EMBL:SPC8854... [more] |
| A0A7J7NNB4 | E-Value: 1.478e-149, PID: 72.64 | (Uncharacterized protein {ECO:0000313|EMBL:KAF6168... [more] |
| A0A5B7ALH4 | E-Value: 1.573e-149, PID: 75.68 | (Uncharacterized protein {ECO:0000313|EMBL:MPA5756... [more] |
| A0A6J0LBV7 | E-Value: 4.535e-149, PID: 74.40 | (calnexin homolog 1 {ECO:0000313|RefSeq:XP_0184571... [more] |
| A0A200R3N5 | E-Value: 5.893e-149, PID: 79.18 | (Calreticulin/calnexin {ECO:0000313|EMBL:OVA17329.... [more] |
| A0A5B7C3F8 | E-Value: 7.488e-149, PID: 76.69 | (Putative Calnexin-1-like protein {ECO:0000313|EMB... [more] |
| A0A5B7C4N9 | E-Value: 2.483e-146, PID: 76.01 | (Putative Calnexin-1-like protein {ECO:0000313|EMB... [more] |
| A0A565CV96 | E-Value: 7.826e-146, PID: 73.88 | (Uncharacterized protein {ECO:0000313|EMBL:VVB1762... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |