Resource Type:
Polypeptide
Name:
DCAR_023423
Identifier:
DCAR_023423.mRNA-protein
Sequence:
MASKNKRKKAKNKNQNNLQGESKPHCLPPKQDHIIKTETEKLFERVKAKV
EALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSE
RAEVTADTDPLLVSDKVQNLANIKVPQPVSNSGSDPKILKRENDDNTGLK
PKKKMKKKASNIKIQTLLKQSDDSSPIVGLGPASIDPVKESSAQELPECE
VPQPVSNSGSNPRIFSSKNEGSTVLKPKKVTMKKKKKKTTNIKIETLLEN
SSNISPIVGCDPSSFNPVKESLDQDLPDCEVPQPVSNSGSIPKILNCENN
TTNIKMQTLLEQSANTSTVVGCGPASIDPVKESLVQDLPECEVPHPMSNS
GSNPKILISENDNSTALKPKKKTTDVNIQTRLEQSANTSPIVGFGPASFD
PVTESLDQELPECKVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDIKI
QKNIEQSATASPVVAFGRGIFDPVEESLVQELPECEVPQPVSTSGCDPRI
SKCENDDSTVLKPNQKTTDNKIQTNIEQSATTSPVVAFGRGIFDPVEESV
VQELPECETYPEHSANTSSIVGFGPDRFDPVKESLVQELPECENIKLESL
RKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSAST
PDKAYPLEKIIEKEEWDCLGDLLNIPRGAKLSPEAYPSFVRNRVHKLEKI
QDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQA
MFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETA
IKYYKQLGCIPRPTTAGLLVELPVPLKFPVPETSSTAN*
Sequence Length:
839
Sequence Checksum:
906d757d2d314a26670b43f3e27b479d
View location in JBrowse:
Organism:
Relationship:
| Relationships |
|---|
| The polypeptide, DCAR_023423, derives from mRNA, DCAR_023423. |
Blast Results:
The following BLAST results are available for this feature:
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5BIT8 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8541672.1}))
HSP 1 Score: 215.312 bits (547), Expect = 4.346e-59
Identity = 151/397 (38.04%), Postives = 213/397 (53.65%), Query Frame = 0
HSP 2 Score: 50.0618 bits (118), Expect = 1.406e-3
Identity = 37/103 (35.92%), Postives = 54/103 (52.43%), Query Frame = 0
Match: A0A5J5BIT8 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8541672.1}))
HSP 1 Score: 215.312 bits (547), Expect = 4.346e-59
Identity = 151/397 (38.04%), Postives = 213/397 (53.65%), Query Frame = 0
Query: 461 SPVVAFGRGIFDPVEESLVQELPECEVPQPVSTSGCDPRISKCE---NDDSTVLKPNQKTTDNKIQTNIEQSATTSPVVAFGRGIFDPVEESVVQELPECETYPEHSANTSSI---VGFGPDRFDPVKESLVQELPECE---------------------NIKLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
SP+V + FDP++ L + E E QP R+ + E N V+ PN D + TN A + V + G+ D +++ + P S + VG G D D +L++E E + KL++LR+ DP ++DL++ I E +K+N A + + N NIP +D+SA+TP YPL+KII K EWD L D+ + + GA+++P+AYPSFV NRV+ LE I+DE EK+T A +L YITHLIK+KDKH G K K PSI+ +KF AMFS+S S+ L KR+LL++YVLVL+LF DG++S+ DI KDLK+ T +Y+ LGC LL LPVPLKFP
Sbjct: 57 SPLVGYFPSGFDPLKVDLTIPVSEEETQQPQPPPQARVRVFRHEKRNNRLQLVVSPNGSQVDF-VGTNYSGEAAAAQVCTYALGVLDKQAQTL-------QIIPITSNKIFRLEPRVG-GSDLSDNEPSNLLKEELSTEKKADKVRDLTLKYSTKKLINKSKKLDALRQKEDPRYQEDLDRKI----ERIKINNEALGSVGSYNTRNIPPHDISATTPQMVYPLDKIIFKGEWDYLLDIFELSQVGAEVAPDAYPSFVCNRVYLLEAIEDEEEKKTFACILSYITHLIKFKDKHSMGGAAS-AKYPKTPSILFQKFSAMFSDSDSKRLLDEKRDLLLSYVLVLTLFADGFRSDPADIAKDLKMTAITLRPHYEYLGCKFVREDQLLLATLPVPLKFP 439
HSP 2 Score: 50.0618 bits (118), Expect = 1.406e-3
Identity = 37/103 (35.92%), Postives = 54/103 (52.43%), Query Frame = 0
Query: 23 KPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSE-RAEVTADTDPLLVSDKVQNLANIK 124
K L K+D + + ++ ER+K EAL + S N +IP HD SA+TP+ L+K I G D L D+ +S+ AEV D P V ++V L I+
Sbjct: 210 KLDALRQKEDPRYQEDLDRKIERIKINNEALGSVGSYNTRNIPPHDISATTPQMVYPLDKIIFKGEWDYLLDIFELSQVGAEVAPDAYPSFVCNRVYLLEAIE 312
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A059BUL2 ((Uncharacterized protein {ECO:0000313|EMBL:KCW69807.1}))
HSP 1 Score: 207.994 bits (528), Expect = 8.603e-57
Identity = 109/235 (46.38%), Postives = 158/235 (67.23%), Query Frame = 0
HSP 2 Score: 37.7354 bits (86), Expect = 9.976e+0
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = 0
Match: A0A059BUL2 ((Uncharacterized protein {ECO:0000313|EMBL:KCW69807.1}))
HSP 1 Score: 207.994 bits (528), Expect = 8.603e-57
Identity = 109/235 (46.38%), Postives = 158/235 (67.23%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLN-IPRGAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
K++SLR+ DP S+KDL++ I +EV +N A ++ + A NIP Y+ SA+ P AYPLEKII K EWD + D+ + G K+SP+AYP+FV NR+HKL ++QDE EK T+ +L YI HLIK+KD+H + + K+HK PSI+ +KF MFS + S+ LP K +LL++YVLVL+LF D ++++ DI KDL++ + T ++++ LGC L +LPVPLKFP
Sbjct: 178 KMQSLRQEDDPNSQKDLDRKI----KEVAINKEALESTDAHIARNIPPYNSSATVPKDAYPLEKIILKGEWDYIKDIFQFLEEGQKVSPDAYPTFVCNRIHKLGEMQDEMEKETLGCILSYINHLIKFKDQHSADGASS-SKNHKIPSILRQKFSTMFSNAESKWLPSEKIDLLISYVLVLTLFADDFRTDPTDIAKDLRMSSVTIRQHFEHLGCKFSREKNVLYAKLPVPLKFP 407
HSP 2 Score: 37.7354 bits (86), Expect = 9.976e+0
Identity = 30/120 (25.00%), Postives = 58/120 (48.33%), Query Frame = 0
Query: 6 KRKKAKNKNQNNLQGESKPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSERAE-VTADTDPLLVSDKVQNLANIK 124
KR ++K +L+ E P+ + + ++ + V EAL++ + A +IP +++SA+ P+ A LEK I G D + D+ E + V+ D P V +++ L ++
Sbjct: 170 KRSVTQDKKMQSLRQEDDPNS---------QKDLDRKIKEVAINKEALESTDAHIARNIPPYNSSATVPKDAYPLEKIILKGEWDYIKDIFQFLEEGQKVSPDAYPTFVCNRIHKLGEMQ 280
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6QFD7 ((DNA-directed RNA polymerase I subunit rpa49 like {ECO:0000313|EMBL:PSS07332.1}))
HSP 1 Score: 207.994 bits (528), Expect = 2.622e-56
Identity = 150/418 (35.89%), Postives = 221/418 (52.87%), Query Frame = 0
HSP 2 Score: 48.1358 bits (113), Expect = 5.808e-3
Identity = 36/103 (34.95%), Postives = 59/103 (57.28%), Query Frame = 0
HSP 3 Score: 45.8246 bits (107), Expect = 3.075e-2
Identity = 32/110 (29.09%), Postives = 54/110 (49.09%), Query Frame = 0
HSP 4 Score: 40.817 bits (94), Expect = 1.200e+0
Identity = 19/47 (40.43%), Postives = 34/47 (72.34%), Query Frame = 0
Match: A0A2R6QFD7 ((DNA-directed RNA polymerase I subunit rpa49 like {ECO:0000313|EMBL:PSS07332.1}))
HSP 1 Score: 207.994 bits (528), Expect = 2.622e-56
Identity = 150/418 (35.89%), Postives = 221/418 (52.87%), Query Frame = 0
Query: 444 KTTDIKIQKNIEQSATASPVVAFGRGIFDPVEESLV---QELPECEVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDNKIQTNIEQSATTSPVVAFGRGIFDPVEESVVQELPECETYPEHSANTSSI---VGFGPDRF--DPVKES-LVQELPECENIKL----------------------ESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
++ D+KI+ E + SP+V + FDP+ V +E P+ EV + RIS+ + V+ PN D + TN AT + + G+ D +++ + P S + VG GPD +P KE V+E P E K ESL++ DP ++ DL+K I E VK+N A ++A + +A NIP +D SA TP AYPL+KII EWD L D++ + + GA+L+ YPSFV NR+H+LE I+DE EK+T+A V YI HL+K+KD++ G + HK PSI+ +KF MF++S ++ L K +LL++YVLVL+L DG++S+ DI +DLK+ T +Y+ LGC LL LPVPL+FP
Sbjct: 55 ESIDVKIETISEHANKISPLVGYFPSSFDPLRHHEVAADEEHPQTEV----RVFRHEKRISRLQ----LVVSPNGSQVDF-VGTNYSGEATAPQLCTYALGVLDKQSQTL-------KIVPIASNKIFRLEPRVG-GPDLSNKEPSKEEPTVEEEPAAEKRKADKIRDLTLMYSTKKTINQAKKRESLQQKEDPETQADLDKKI----EGVKINKEALESASSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLSDIIELLQFGAELASNDYPSFVCNRLHRLEHIEDEVEKKTLACVFSYINHLVKFKDRNSFGGSSS-ATFHKMPSILSQKFSTMFADSDAKWLADDKTDLLISYVLVLTLIADGFRSDPSDIARDLKMTAITIRPHYEHLGCKLVRDGRVLLATLPVPLQFP 450
HSP 2 Score: 48.1358 bits (113), Expect = 5.808e-3
Identity = 36/103 (34.95%), Postives = 59/103 (57.28%), Query Frame = 0
Query: 23 KPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSER-AEVTADTDPLLVSDKVQNLANIK 124
K L K+D + + +K E VK EAL++A S +A +IP HD+SA TP+ A L+K I +G D L D+ + + AE+ ++ P V +++ L +I+
Sbjct: 221 KRESLQQKEDPETQADLDKKIEGVKINKEALESASSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLSDIIELLQFGAELASNDYPSFVCNRLHRLEHIE 323
HSP 3 Score: 45.8246 bits (107), Expect = 3.075e-2
Identity = 32/110 (29.09%), Postives = 54/110 (49.09%), Query Frame = 0
Query: 372 KTTDVNIQTRLEQSANTSPIVGFGPASFDPVTE---SLDQELPECKVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDIKIQKNIEQSATASPVVAFGRGIFDPVEESL 478
++ DV I+T E + SP+VG+ P+SFDP+ + D+E P+ + V + RIS+ + V+ PN D + N ATA + + G+ D ++L
Sbjct: 55 ESIDVKIETISEHANKISPLVGYFPSSFDPLRHHEVAADEEHPQTE----VRVFRHEKRISRLQ----LVVSPNGSQVDF-VGTNYSGEATAPQLCTYALGVLDKQSQTL 155
HSP 4 Score: 40.817 bits (94), Expect = 1.200e+0
Identity = 19/47 (40.43%), Postives = 34/47 (72.34%), Query Frame = 0
Query: 238 KTTNIKIETLLENSSNISPIVGCDPSSFNPVKE---SLDQDLPDCEV 281
++ ++KIET+ E+++ ISP+VG PSSF+P++ + D++ P EV
Sbjct: 55 ESIDVKIETISEHANKISPLVGYFPSSFDPLRHHEVAADEEHPQTEV 101
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6R0C1 ((DNA-directed RNA polymerase I subunit rpa49 like {ECO:0000313|EMBL:PSS18082.1}))
HSP 1 Score: 207.608 bits (527), Expect = 6.744e-56
Identity = 165/495 (33.33%), Postives = 243/495 (49.09%), Query Frame = 0
HSP 2 Score: 49.6766 bits (117), Expect = 2.169e-3
Identity = 37/103 (35.92%), Postives = 59/103 (57.28%), Query Frame = 0
HSP 3 Score: 38.5058 bits (88), Expect = 6.836e+0
Identity = 46/182 (25.27%), Postives = 71/182 (39.01%), Query Frame = 0
Match: A0A2R6R0C1 ((DNA-directed RNA polymerase I subunit rpa49 like {ECO:0000313|EMBL:PSS18082.1}))
HSP 1 Score: 207.608 bits (527), Expect = 6.744e-56
Identity = 165/495 (33.33%), Postives = 243/495 (49.09%), Query Frame = 0
Query: 375 DVNIQTRLEQSANTSPIVGFGPASFDPV-TESLDQELPECKVPQPVSTSGCDPRISKCENDDSTVLKP------NQKTTDIKIQKNIEQSATASPVVAFGRGIFDPVEESLV-QELPECEVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDNKIQTNIEQSATTSPVVAFGRGIFD---------PVEESVVQELPECETYPEHSANTSSIVGFGPDRFDPVKESLVQELPECENIKL----------------------ESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
DV I+T E N SP+ GF P V TE LPE V T+ + + +D+ K N++ D+KIQ E SP V + FDP+ V E V + R S+ + V+ PN D + TN AT + + G+ D P+ + + L E S + + +P+ E E P E K ESL++ DP +++DL+K I E VK+N A ++A + +A NIP +D SA TP AYPL+KII EWD L D+L + + GA+L+ + +P+FV NR+H+LE I+DE EK+T+A V YI HLIK+KD++ G + VK HK P+I+ +KF +F++S ++ L K++LL++YVLVL+L DG++S+ DI +DLK+ T +Y+ LGC LL LPVPL+FP
Sbjct: 14 DVTIETLSEHPNNVSPVAGFDPHRNHHVATEEEAPPLPE------VGTAETEEKPETLVEEDTKPKKKKKKKKKNEENIDVKIQTLGEHPNKISPFVGYFPSGFDPLRHHQVAAEEEGPPPLPEVRVFRHEKRTSRLQ----LVVSPNGSQVDF-VGTNYSGEATAPQLCTYALGVLDKQSQTLKIVPIASNKIFRL-------EPRVGGSDLSNKETSKEEPIAE----EEPTAEKRKADKIRDLTFMYSTKKTINQAKKRESLQQKEDPETQEDLDKKI----EGVKINKEALESASSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLLDILELLQFGAELASDDFPTFVCNRLHRLEHIEDEVEKKTLACVFSYINHLIKFKDRNSIGASSS-VKFHKIPTILSQKFSTLFADSDAKWLADDKKDLLISYVLVLTLIADGFRSDPSDIARDLKMTAITIRPHYEHLGCKLVREGRVLLATLPVPLQFP 481
HSP 2 Score: 49.6766 bits (117), Expect = 2.169e-3
Identity = 37/103 (35.92%), Postives = 59/103 (57.28%), Query Frame = 0
Query: 23 KPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSER-AEVTADTDPLLVSDKVQNLANIK 124
K L K+D + + +K E VK EAL++A S +A +IP HD+SA TP+ A L+K I +G D L D+ + + AE+ +D P V +++ L +I+
Sbjct: 252 KRESLQQKEDPETQEDLDKKIEGVKINKEALESASSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLLDILELLQFGAELASDDFPTFVCNRLHRLEHIE 354
HSP 3 Score: 38.5058 bits (88), Expect = 6.836e+0
Identity = 46/182 (25.27%), Postives = 71/182 (39.01%), Query Frame = 0
Query: 303 NIKMQTLLEQSANTSTVVGCGPASIDPVK-ESLVQDLPECEVPHPMSNSGSNPKILISEN----DNSTALKPKKKTTDVNIQTRLEQSANTSPIVGFGPASFDPVT-ESLDQELPECKVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDIKIQKNIEQSATASPVVAFGRGIFDPVEESL 478
++ ++TL E N S V G P V E LPE + + P+ L+ E+ K ++ DV IQT E SP VG+ P+ FDP+ + E V + R S+ + V+ PN D + N ATA + + G+ D ++L
Sbjct: 14 DVTIETLSEHPNNVSPVAGFDPHRNHHVATEEEAPPLPEVGT----AETEEKPETLVEEDTKPKKKKKKKKKNEENIDVKIQTLGEHPNKISPFVGYFPSGFDPLRHHQVAAEEEGPPPLPEVRVFRHEKRTSRLQ----LVVSPNGSQVDF-VGTNYSGEATAPQLCTYALGVLDKQSQTL 186
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1U8BC37 ((DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|RefSeq:XP_010273794.1}))
HSP 1 Score: 202.601 bits (514), Expect = 6.168e-55
Identity = 111/240 (46.25%), Postives = 159/240 (66.25%), Query Frame = 0
HSP 2 Score: 43.5134 bits (101), Expect = 1.651e-1
Identity = 38/126 (30.16%), Postives = 59/126 (46.83%), Query Frame = 0
Match: A0A1U8BC37 ((DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|RefSeq:XP_010273794.1}))
HSP 1 Score: 202.601 bits (514), Expect = 6.168e-55
Identity = 111/240 (46.25%), Postives = 159/240 (66.25%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKH-LSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFPVPET 833
K+ESL++ DP+S+ ++K I E +N A + A A N+P +D +A+TPDKAYPL KII K EWD L D+L + + GA +S +AYPSFV NR++KLE+IQDE EK+ +A +L YITHLIK+KD+ + G + K HKFP II +KF +F+ S + L K++LL++YVLVL+LF+D + ++ DI KDL++ + +Y+ LGC L+V LPVPL+FP P T
Sbjct: 168 KMESLKQKEDPISQDAVDKKI----EGFVINKDALETAAVYTARNVPPHDPTATTPDKAYPLNKIIFKGEWDYLLDVLELLQSGATISSDAYPSFVCNRIYKLEEIQDEMEKKRLACILSYITHLIKFKDQRSVDGASS--AKYHKFPPIILQKFLTLFANSETYRLADDKQDLLISYVLVLTLFIDKFSTDMSDIAKDLRMTPVSLRLHYQNLGCKLSRVDKLLMVTLPVPLQFPKPRT 401
HSP 2 Score: 43.5134 bits (101), Expect = 1.651e-1
Identity = 38/126 (30.16%), Postives = 59/126 (46.83%), Query Frame = 0
Query: 7 RKKAKNKNQNNLQGESKP-------HCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHD-LPNVSERAEVTADTDPLLVSDKVQNLANIK 124
+K K ++ NL G K L K+D I + +K E +AL+ A A ++P HD +A+TP+KA L K I G D L D L + A +++D P V +++ L I+
Sbjct: 145 QKADKIRDLTNLYGTKKSISRAKKMESLKQKEDPISQDAVDKKIEGFVINKDALETAAVYTARNVPPHDPTATTPDKAYPLNKIIFKGEWDYLLDVLELLQSGATISSDAYPSFVCNRIYKLEEIQ 270
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YWH5 ((Putative DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|EMBL:MPA36045.1}))
HSP 1 Score: 203.371 bits (516), Expect = 8.647e-55
Identity = 111/236 (47.03%), Postives = 158/236 (66.95%), Query Frame = 0
HSP 2 Score: 59.3066 bits (142), Expect = 1.898e-6
Identity = 43/129 (33.33%), Postives = 70/129 (54.26%), Query Frame = 0
Match: A0A5B6YWH5 ((Putative DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|EMBL:MPA36045.1}))
HSP 1 Score: 203.371 bits (516), Expect = 8.647e-55
Identity = 111/236 (47.03%), Postives = 158/236 (66.95%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKH-LSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
KLESL + D K++L++ I + +K+N A + NA NIP +D SA+TP AYPL+KII EWD L D+L + + GA+++ + +PSFV NRV+KLE I+DE EK+ +A +L YITHLIK+KDKH + G + K HK PSI+ +KF AMF++S S+ L K +LL++Y+LVL+LFVDG++S+ DI +DL++ T +Y+ LGC LL LPVPL+FP
Sbjct: 213 KLESLHQKEDAKYKEELDRKI----KRIKINNEALGSGGTYNARNIPPHDTSATTPQMAYPLDKIIFSGEWDYLLDILELSQVGAEVALDVFPSFVCNRVNKLEDIEDEMEKQQLACILSYITHLIKFKDKHSMDGASS--AKYHKIPSILFQKFSAMFADSDSKRLLDEKNDLLLSYILVLTLFVDGFRSDPSDIARDLRMTAITLRPHYEYLGCKLVRKGQLLLATLPVPLQFP 442
HSP 2 Score: 59.3066 bits (142), Expect = 1.898e-6
Identity = 43/129 (33.33%), Postives = 70/129 (54.26%), Query Frame = 0
Query: 1 MASKNKRKKAKN-----KNQNNLQGESKPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSE-RAEVTADTDPLLVSDKVQNLANI 123
++++NK K ++ + ++ K L K+D K E ++ +R+K EAL + + NA +IP HDTSA+TP+ A L+K I +G D L D+ +S+ AEV D P V ++V L +I
Sbjct: 186 LSTENKADKVRDLTLMYSTKKSINKAKKLESLHQKEDAKYKEELDRKIKRIKINNEALGSGGTYNARNIPPHDTSATTPQMAYPLDKIIFSGEWDYLLDILELSQVGAEVALDVFPSFVCNRVNKLEDI 314
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YW05 ((Putative DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|EMBL:MPA36044.1}))
HSP 1 Score: 202.601 bits (514), Expect = 1.335e-54
Identity = 111/236 (47.03%), Postives = 158/236 (66.95%), Query Frame = 0
HSP 2 Score: 59.3066 bits (142), Expect = 1.745e-6
Identity = 43/129 (33.33%), Postives = 70/129 (54.26%), Query Frame = 0
Match: A0A5B6YW05 ((Putative DNA-directed RNA polymerase I subunit rpa49 {ECO:0000313|EMBL:MPA36044.1}))
HSP 1 Score: 202.601 bits (514), Expect = 1.335e-54
Identity = 111/236 (47.03%), Postives = 158/236 (66.95%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKH-LSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
KLESL + D K++L++ I + +K+N A + NA NIP +D SA+TP AYPL+KII EWD L D+L + + GA+++ + +PSFV NRV+KLE I+DE EK+ +A +L YITHLIK+KDKH + G + K HK PSI+ +KF AMF++S S+ L K +LL++Y+LVL+LFVDG++S+ DI +DL++ T +Y+ LGC LL LPVPL+FP
Sbjct: 207 KLESLHQKEDAKYKEELDRKI----KRIKINNEALGSGGTYNARNIPPHDTSATTPQMAYPLDKIIFSGEWDYLLDILELSQVGAEVALDVFPSFVCNRVNKLEDIEDEMEKQQLACILSYITHLIKFKDKHSMDGASS--AKYHKIPSILFQKFSAMFADSDSKRLLDEKNDLLLSYILVLTLFVDGFRSDPSDIARDLRMTAITLRPHYEYLGCKLVRKGQLLLATLPVPLQFP 436
HSP 2 Score: 59.3066 bits (142), Expect = 1.745e-6
Identity = 43/129 (33.33%), Postives = 70/129 (54.26%), Query Frame = 0
Query: 1 MASKNKRKKAKN-----KNQNNLQGESKPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSE-RAEVTADTDPLLVSDKVQNLANI 123
++++NK K ++ + ++ K L K+D K E ++ +R+K EAL + + NA +IP HDTSA+TP+ A L+K I +G D L D+ +S+ AEV D P V ++V L +I
Sbjct: 180 LSTENKADKVRDLTLMYSTKKSINKAKKLESLHQKEDAKYKEELDRKIKRIKINNEALGSGGTYNARNIPPHDTSATTPQMAYPLDKIIFSGEWDYLLDILELSQVGAEVALDVFPSFVCNRVNKLEDI 308
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J0FGY8 ((Uncharacterized protein {ECO:0000313|EMBL:GFY97177.1}))
HSP 1 Score: 202.601 bits (514), Expect = 2.527e-54
Identity = 108/235 (45.96%), Postives = 159/235 (67.66%), Query Frame = 0
HSP 2 Score: 48.521 bits (114), Expect = 5.063e-3
Identity = 36/103 (34.95%), Postives = 59/103 (57.28%), Query Frame = 0
Match: A0A7J0FGY8 ((Uncharacterized protein {ECO:0000313|EMBL:GFY97177.1}))
HSP 1 Score: 202.601 bits (514), Expect = 2.527e-54
Identity = 108/235 (45.96%), Postives = 159/235 (67.66%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKHLSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
K ESL++ DP +++DL+K I E VK+N A +++ + +A NIP +D SA TP AYPL+KII EWD L D+L + + GA+L+ + +P+FV NR+H+LE I+DE EK+T+A V YI HLIK+KD++ G + VK HK P+I+ +KF MF++S ++ L K++LL++YVLVL+L DG++S+ DI +DLK+ T +Y+ LGC LL LPVPL+FP
Sbjct: 234 KRESLQQKEDPETQEDLDKKI----EGVKINKEALESSSSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLLDILELLQFGAELASDDFPTFVCNRLHRLEHIEDEVEKKTLACVFSYINHLIKFKDRNSIGASSS-VKFHKIPTILSQKFSTMFADSDAKWLADDKKDLLISYVLVLTLIADGFRSDPSDIARDLKMTAITIRPHYEHLGCKLVREGRLLLATLPVPLQFP 463
HSP 2 Score: 48.521 bits (114), Expect = 5.063e-3
Identity = 36/103 (34.95%), Postives = 59/103 (57.28%), Query Frame = 0
Query: 23 KPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSE-RAEVTADTDPLLVSDKVQNLANIK 124
K L K+D + + +K E VK EAL+++ S +A +IP HD+SA TP+ A L+K I +G D L D+ + + AE+ +D P V +++ L +I+
Sbjct: 234 KRESLQQKEDPETQEDLDKKIEGVKINKEALESSSSHSARNIPPHDSSAITPQTAYPLDKIIFSGEWDYLLDILELLQFGAELASDDFPTFVCNRLHRLEHIE 336
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A3Q7GSJ7 ((Uncharacterized protein {ECO:0000313|EnsemblPlants:Solyc06g053330.3.1}))
HSP 1 Score: 199.134 bits (505), Expect = 1.227e-53
Identity = 108/237 (45.57%), Postives = 153/237 (64.56%), Query Frame = 0
HSP 2 Score: 40.0466 bits (92), Expect = 1.905e+0
Identity = 27/76 (35.53%), Postives = 44/76 (57.89%), Query Frame = 0
Match: A0A3Q7GSJ7 ((Uncharacterized protein {ECO:0000313|EnsemblPlants:Solyc06g053330.3.1}))
HSP 1 Score: 199.134 bits (505), Expect = 1.227e-53
Identity = 108/237 (45.57%), Postives = 153/237 (64.56%), Query Frame = 0
Query: 596 KLESLRKNRDPVSKKDLEKSI-DSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKH-LSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
KL+SLR+ D ++++ +++I +I +E VA +A N IP +D+ A+ P AYPL++II K EWD L D+ + GA+++P+ YPSFVRNR +KLE IQDE EK+ +A +L +ITHL+K+KDKH + G + K HKFP I+ +KF +MFS S S+ LP K LL+NYVLVL+LF D ++S+ DI KDL+I +Y+ LGC +L LPVPL FP
Sbjct: 173 KLDSLRQQEDTGNQEEFDRNIAGAINKEALEVTVATDSARN-----IPPHDLDATIPQLAYPLDRIISKGEWDFLLDIFELTEAGAEMTPDLYPSFVRNRSYKLEHIQDEDEKKRLAGILSFITHLVKFKDKHSMDGVSS--AKHHKFPGILSQKFTSMFSISDSKRLPEEKVTLLINYVLVLTLFADDFRSDPSDIAKDLRINAVALRPFYEYLGCKLVREKNIVLATLPVPLVFP 402
HSP 2 Score: 40.0466 bits (92), Expect = 1.905e+0
Identity = 27/76 (35.53%), Postives = 44/76 (57.89%), Query Frame = 0
Query: 51 EALKAAISTN-AGSIPFHDTSASTPEKAESLEKKIENGGPDDLHDLPNVSER-AEVTADTDPLLVSDKVQNLANIK 124
EAL+ ++T+ A +IP HD A+ P+ A L++ I G D L D+ ++E AE+T D P V ++ L +I+
Sbjct: 200 EALEVTVATDSARNIPPHDLDATIPQLAYPLDRIISKGEWDFLLDIFELTEAGAEMTPDLYPSFVRNRSYKLEHIQ 275
BLAST of DCAR_023423 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6A4MLF4 ((Uncharacterized protein {ECO:0000313|EMBL:KAE9467941.1}) (Fragment))
HSP 1 Score: 199.519 bits (506), Expect = 1.557e-53
Identity = 139/405 (34.32%), Postives = 208/405 (51.36%), Query Frame = 0
HSP 2 Score: 46.595 bits (109), Expect = 1.523e-2
Identity = 39/117 (33.33%), Postives = 60/117 (51.28%), Query Frame = 0
Match: A0A6A4MLF4 ((Uncharacterized protein {ECO:0000313|EMBL:KAE9467941.1}) (Fragment))
HSP 1 Score: 199.519 bits (506), Expect = 1.557e-53
Identity = 139/405 (34.32%), Postives = 208/405 (51.36%), Query Frame = 0
Query: 448 IKIQKNIEQSATASPVVAFGRGIFDPVEESLVQELPECEVPQPVSTSGCDPRISKCENDDSTVLKPNQKTTDNKIQTNIEQSATTSPVVAFGRGIFDPVEESVVQELPECETYPEHSANTSS----IVGFGPDRFDPVKESLVQELPECE-----------------NIKLESLRKNRDPVSKKDLEKSIDSIPEEVKVNIVAHKAAENMNAPNIPFYDMSASTPDKAYPLEKIIEKEEWDCLGDLLNIPR-GAKLSPEAYPSFVRNRVHKLEKIQDEHEKRTVASVLQYITHLIKYKDKH-LSGCYNKFVKDHKFPSIIEEKFQAMFSESSSEALPPAKRELLVNYVLVLSLFVDGYKSNAKDITKDLKIKTETAIKYYKQLGCIPRPTTAGLLVELPVPLKFP 829
+KI+ E SP V + FDP++ Q E PQ R K N V+ PN + + TN AT + + G+ D ++L + P S + G +P KE + L + E N K +SL + DP ++++L++ E VK+N A ++A ++ N P +D SA+TP AYPL+KII K EWD L D+L + + GA+++P+ YPSF+ NR+H+LE I+DE EK+ +A V YI HL+K+KDK+ + GC + K HK PSI+ +KF MF S + L KR+LL++YVLVL+L DG+++ DITKDL++ + +++ LGC + LL LPVPL FP
Sbjct: 37 VKIETLGENPNKVSPFVGYFPSGFDPLKN---QATAAGEPPQATVRIF---RHEKRTNRLQLVVSPNNGFQVDFVGTNYSGEATAPQLCTYALGVLD-------KQLQTLKIVPIASNKIFRLEPRVGGLDLSVKEPSKEEELTTLKKFEKREALDDIYGTKKAINRNKKRKSLSRKDDPEAQENLDRKF----EGVKINKEALESAGMHSSRNTPPHDSSATTPQMAYPLDKIILKGEWDYLVDILELFQDGAEIAPDGYPSFICNRLHRLESIEDEVEKKRLACVFSYINHLVKFKDKYSMEGCSS--AKHHKIPSILSQKFSTMFGYSDKKRLEDEKRDLLISYVLVLTLIADGFRTAVPDITKDLRLDSVAVKSHFENLGCKLMIKSKVLLATLPVPLVFP 422
HSP 2 Score: 46.595 bits (109), Expect = 1.523e-2
Identity = 39/117 (33.33%), Postives = 60/117 (51.28%), Query Frame = 0
Query: 8 KKAKNKNQNNLQGESKPHCLPPKQDHIIKTETEKLFERVKAKVEALKAAISTNAGSIPFHDTSASTPEKAESLEKKIENGGPDDLHD-LPNVSERAEVTADTDPLLVSDKVQNLANI 123
KKA N+N+ K L K D + ++ FE VK EAL++A ++ + P HD+SA+TP+ A L+K I G D L D L + AE+ D P + +++ L +I
Sbjct: 185 KKAINRNK-------KRKSLSRKDDPEAQENLDRKFEGVKINKEALESAGMHSSRNTPPHDSSATTPQMAYPLDKIILKGEWDYLVDILELFQDGAEIAPDGYPSFICNRLHRLESI 294
| Match Name | Stats | Description |
|---|---|---|
| A0A5J5BIT8 | E-Value: 4.346e-59, PID: 38.04 | (Uncharacterized protein {ECO:0000313|EMBL:KAA8541... [more] |
| A0A059BUL2 | E-Value: 8.603e-57, PID: 46.38 | (Uncharacterized protein {ECO:0000313|EMBL:KCW6980... [more] |
| A0A2R6QFD7 | E-Value: 2.622e-56, PID: 35.89 | (DNA-directed RNA polymerase I subunit rpa49 like ... [more] |
| A0A2R6R0C1 | E-Value: 6.744e-56, PID: 33.33 | (DNA-directed RNA polymerase I subunit rpa49 like ... [more] |
| A0A1U8BC37 | E-Value: 6.168e-55, PID: 46.25 | (DNA-directed RNA polymerase I subunit rpa49 {ECO:... [more] |
| A0A5B6YWH5 | E-Value: 8.647e-55, PID: 47.03 | (Putative DNA-directed RNA polymerase I subunit rp... [more] |
| A0A5B6YW05 | E-Value: 1.335e-54, PID: 47.03 | (Putative DNA-directed RNA polymerase I subunit rp... [more] |
| A0A7J0FGY8 | E-Value: 2.527e-54, PID: 45.96 | (Uncharacterized protein {ECO:0000313|EMBL:GFY9717... [more] |
| A0A3Q7GSJ7 | E-Value: 1.227e-53, PID: 45.57 | (Uncharacterized protein {ECO:0000313|EnsemblPlant... [more] |
| A0A6A4MLF4 | E-Value: 1.557e-53, PID: 34.32 | (Uncharacterized protein {ECO:0000313|EMBL:KAE9467... [more] |
Pages
back to topAnalysis:
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |