XM_017378500.1

Resource Type: 
Polypeptide
Name: 
XM_017378500.1
Identifier: 
XM_017378500.1-protein
Sequence: 
MVKASHIREHQVLPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGS
VTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEA
ALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAKNQESSS
GLNSSKQAAESAGEETEDINELTNYRHLLKL
Sequence Length: 
181
Sequence Checksum: 
280b8768eecda7173dc1ddf1b7846d5e
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, XM_017378500.1, derives from mRNA, XM_017378500.1.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2022-01-09
Analysis Name: NCBI peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 10
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6A1W9W8 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 185.267 bits (469), Expect = 2.612e-57
Identity = 94/157 (59.87%), Postives = 112/157 (71.34%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAK-NQESSSGLNSSKQAAESAGEETED 168
            LP NENDS DMV+ QVL+EA  +      Q+   N  S  +P R I KK HYRGVRRRPWGKYAAEIRDS+RQGAR+WLGTF TAEEAALAYDRAAF+MRGAKALLNFP +V A SA ++F PN +   L K + E S+G +S++    S   E+E 
Sbjct:   36 LPFNENDSHDMVIYQVLNEAVTLNSSCAPQRQQSNTSSSLEPVRNIGKK-HYRGVRRRPWGKYAAEIRDSSRQGARIWLGTFTTAEEAALAYDRAAFRMRGAKALLNFPAEVVAASAFQRFHPNSSLVSLNKQDSEPSTGSSSTQSIGTSKTLESES 191    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2K3KXT4 ((Pathogenesis-related genes transcriptional activator pti5-like protein {ECO:0000313|EMBL:PNX71092.1}))

HSP 1 Score: 182.185 bits (461), Expect = 1.185e-56
Identity = 90/143 (62.94%), Postives = 108/143 (75.52%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAKNQESSSGLNSS 155
            LPLNENDSQDMV+ QVL+EA  +T     Q++H    S  +PTR I+KK HYRGVRRRPWGKYAAEIRDSAR GARVWLGTF+TAEEAA+AYDRAAFKMRG+KALLNFP ++ A ++++    N   KL   N +S S  +S 
Sbjct:   11 LPLNENDSQDMVIYQVLNEANNLTNTFMQQRNHHQALSGLEPTRAITKK-HYRGVRRRPWGKYAAEIRDSARHGARVWLGTFQTAEEAAMAYDRAAFKMRGSKALLNFPAEIVAAASMQMEFSNSKLKLNNSNHDSESSGSSC 152    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6QA93 ((Pathogenesis-related transcriptional activator like {ECO:0000313|EMBL:PSS04821.1}))

HSP 1 Score: 182.185 bits (461), Expect = 3.347e-56
Identity = 86/132 (65.15%), Postives = 102/132 (77.27%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAK 144
            LPLNENDSQDMVL  +L+E+     P++  ++  N   V +PT  I +KKHYRGVRRRPWGKYAAEIRDS R GAR WLGTFETAEEAALAYDRAAF MRGAKALLNFP +VGA S+ ++F PN + + L +
Sbjct:    9 LPLNENDSQDMVLYHLLNESNGQNTPIFPSKTQSNARKVLEPTGTI-EKKHYRGVRRRPWGKYAAEIRDSTRNGARKWLGTFETAEEAALAYDRAAFSMRGAKALLNFPAEVGAESSKQRFTPNLSSRRLDR 139    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7CC35 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 180.644 bits (457), Expect = 4.664e-56
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAIT---------LPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAKNQESSSGLNSSKQAAESAGEET 166
            LPLNENDSQDMV+ ++L EA  +           P  +Q   RN    T PT   + KKHYRGVRRRPWGK+AAEIRDSAR GARVWLGTFETAEEAALAYDRAAF+MRGAKALLNFP Q+ A S++++F PN     L+ NQ S+S   S+K +  +AG E+
Sbjct:    9 LPLNENDSQDMVIFEILKEANVLNEANAPNSSFCPPRNQSIARNVTGRT-PT---AAKKHYRGVRRRPWGKFAAEIRDSARNGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFPAQIVAASSVQRFNPN-----LSDNQRSNSSSGSNKLSTGTAGSES 162    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J0D9C2 ((Related to AP2 6l {ECO:0000313|EMBL:GFS30265.1}))

HSP 1 Score: 181.03 bits (458), Expect = 8.837e-56
Identity = 85/132 (64.39%), Postives = 102/132 (77.27%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAK 144
            LPLNENDSQDMVL  +L+E+     P++  ++  N   V +PT  I +KKHYRGVRRRPWGKYAAEIRDS R GAR WLGTFETAEEAALAYDRAAF MRG+KALLNFP +VGA S+ ++F PN + + L +
Sbjct:    9 LPLNENDSQDMVLYHLLNESNGQNTPIFPSKTQSNARKVLEPTGTI-EKKHYRGVRRRPWGKYAAEIRDSTRHGARKWLGTFETAEEAALAYDRAAFSMRGSKALLNFPAEVGAESSKQRFSPNLSSRRLDR 139    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J4ZRL4 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 179.104 bits (453), Expect = 2.342e-55
Identity = 91/141 (64.54%), Postives = 105/141 (74.47%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAIT---------LPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAK 144
            LPLNENDSQDMV+ +VL+EA             LP  ++   RN   VT PT  + KK HYRGVRRRPWGK+AAEIRDSAR GARVWLGTFETAEEAALAYDRAAF+MRGAKALLNFP +V A S++++F PN + K L K
Sbjct:    9 LPLNENDSQDMVIYEVLNEANVFNEANALGSSFLPPRNRSMARN---VTGPTPTVGKK-HYRGVRRRPWGKFAAEIRDSARNGARVWLGTFETAEEAALAYDRAAFEMRGAKALLNFPAEVVAASSVQRFNPNLSSKRLDK 145    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A834LW26 ((Uncharacterized protein {ECO:0000313|EMBL:KAF7150314.1}))

HSP 1 Score: 177.178 bits (448), Expect = 3.262e-54
Identity = 91/149 (61.07%), Postives = 108/149 (72.48%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPG--SVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFTFKLLAKNQESSSGLNSSKQAA 159
            LPLNENDSQDMVL Q+LDEA A+    Y  ++  N    +V +PT   + KKHYRGVRRRPWGK+AAEIRDSAR GAR+WLGTFETAEEAALAYDRAAF+MRGAKALLNFP +VGA      F  N + + L     SSS  N ++ ++
Sbjct:   11 LPLNENDSQDMVLYQLLDEANAVNTQFYPSRNQTNTTIQNVLEPTGT-NGKKHYRGVRRRPWGKFAAEIRDSARHGARIWLGTFETAEEAALAYDRAAFRMRGAKALLNFPAEVGAEFPNRWFNRNLSSRKLDNKHGSSSSRNGNETSS 158    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6J1B683 ((pathogenesis-related genes transcriptional activator PTI5 {ECO:0000313|RefSeq:XP_021294703.1}))

HSP 1 Score: 177.563 bits (449), Expect = 4.612e-54
Identity = 94/145 (64.83%), Postives = 111/145 (76.55%), Query Frame = 0
 
Query:    1 MVKASHIREHQVLPLNENDSQDMVLQQVLDEA--QAITLPLYHQ--QSHRNPGSVTD---PTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPNFT 138
            MV+ SH  E+Q LP NENDSQDM + QVL+EA   A+ +  +HQ     RN  S T+   P+R I+KK HYRGVRRRPWGKYAAEIRDS R GARVWLGTFETAEEAALAYDRAAF+MRGAKALLNFP +V A S++++  PN +
Sbjct:   48 MVQVSH-SENQ-LPFNENDSQDMFIYQVLNEANAHAVAINNFHQFHVPPRNQSSATNMLEPSRTIAKK-HYRGVRRRPWGKYAAEIRDSTRNGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFPAEVVAASSVQRLRPNLS 189    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2N9GEN2 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 175.252 bits (443), Expect = 1.180e-53
Identity = 84/124 (67.74%), Postives = 96/124 (77.42%), Query Frame = 0
 
Query:   13 LPLNENDSQDMVLQQVLDEAQAITLPLYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQVGAHSAIEKFEPN 136
            LP NENDSQDM L QVL+EA +I      Q+   N  S  +P   I KK HYRGVRRRPWGKYAAEIRDSAR GARVWLGTF TAEEAA+AYD+AAF+MRGAKALLNFP +V A S++++F PN
Sbjct:    9 LPFNENDSQDMALYQVLNEAMSINSSFVPQRHQTNVSSGLEPAGNIGKK-HYRGVRRRPWGKYAAEIRDSARHGARVWLGTFTTAEEAAMAYDKAAFRMRGAKALLNFPAEVVAASSVQRFNPN 131    
BLAST of XM_017378500.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J7D7U2 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 174.866 bits (442), Expect = 1.180e-53
Identity = 94/166 (56.63%), Postives = 117/166 (70.48%), Query Frame = 0
 
Query:   11 QVLPLNENDSQDMVLQQVLDEAQAITLP-----LYHQQSHRNPGSVTDPTRVISKKKHYRGVRRRPWGKYAAEIRDSARQGARVWLGTFETAEEAALAYDRAAFKMRGAKALLNFPGQ-VGAHSAIEKFEPNFTFKLLAKNQESSSGLNSSKQAAESAGEETEDIN 170
            ++LPLNENDSQDMV+ QVL+EA     P     +   ++H N  S+  P R++ KK HYRGVRRRPWGKYAAEIRDS RQGAR+WLGTFETAEEAALAYD AAF+MRGAKALLNFP + V A S+++KF+P    K   K+   S+   +S+  +ES   E   +N
Sbjct:    8 ELLPLNENDSQDMVIYQVLNEADQSMPPTTSSSVVPPRNHSNTSSILQPARMLGKK-HYRGVRRRPWGKYAAEIRDSTRQGARLWLGTFETAEEAALAYDIAAFRMRGAKALLNFPAEVVVASSSMQKFKPTSRSK---KSDLDSTITETSQSESESGRTEEFSLN 169    
Match NameStatsDescription
A0A6A1W9W8E-Value: 2.612e-57, PID: 59.87AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A2K3KXT4E-Value: 1.185e-56, PID: 62.94(Pathogenesis-related genes transcriptional activa... [more]
A0A2R6QA93E-Value: 3.347e-56, PID: 65.15(Pathogenesis-related transcriptional activator li... [more]
A0A5B7CC35E-Value: 4.664e-56, PID: 58.90AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A7J0D9C2E-Value: 8.837e-56, PID: 64.39(Related to AP2 6l {ECO:0000313|EMBL:GFS30265.1})[more]
A0A5J4ZRL4E-Value: 2.342e-55, PID: 64.54AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A834LW26E-Value: 3.262e-54, PID: 61.07(Uncharacterized protein {ECO:0000313|EMBL:KAF7150... [more]
A0A6J1B683E-Value: 4.612e-54, PID: 64.83(pathogenesis-related genes transcriptional activa... [more]
A0A2N9GEN2E-Value: 1.180e-53, PID: 67.74AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A7J7D7U2E-Value: 1.180e-53, PID: 56.63AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

The RefSeq genome records for Daucus carota subsp. sativus were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results.

View the full report at https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Daucus_carota_subsp._sativus/100/

Data from this analysis can be viewed in JBrowse here.

This analysis is a blastp search of all of the NCBI Daucus carota subsp. sativus Annotation Release 100 polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove DCAR gene predictions from DCAR V1.0 Gene Prediction.
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