MMFGPKEFCMIPPVIMFQACCIYYFYCNRGMYYYHGSNVIKKIKDLIEKI
WSEFWRNRIKCLEVVFQLSMVALTSFLLKDTHTWTAIWIASSAIFAILYS
SYKACVKMKRAQLMPPVNRRYYRVLGYQVIPIHIIYNVASHWNPRGVVID
TLLIVVGNEDVVVPNVAEVLQDLGIQSVPQANAPEVTVQRDGRNQSVADT
SVPEVAIQRDLEDQFVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQ
SAAGEPEVPQKDDEENQSVDNEYATEIVIQKYVEDQSESDAGPSEVAHKD
EEYHDLHMQM
| Relationships |
|---|
| The polypeptide, XM_017371499.1, derives from mRNA, XM_017371499.1. |
Analysis Date: 2022-01-09
Analysis Name: NCBI peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 10
Match: A0A250WPN8 ((Uncharacterized protein {ECO:0000313|EMBL:GAX72814.1}) (Fragment))
HSP 1 Score: 44.2838 bits (103), Expect = 2.595e-2
Identity = 35/108 (32.41%), Postives = 62/108 (57.41%), Query Frame = 0
Query: 183 APEVTVQRDGRNQSVADTSVPEVAIQRDLEDQFVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQKDDEENQSVDNEYATEIVIQKYVEDQSESD 290
A EV +++D V D EVA+++D+EDQF + EVA D + + E+A+++DV+D+ A+ EV + D V++ +A+E+ ++K VED+ S+
Sbjct: 777 ASEVALRKD-----VEDRFASEVALRKDVEDQFAS---EVALRKD-----VEDRFASEVALRKDVEDRFAS---EVALRKD-----VEDRFASEVALRKDVEDRFASE 863
HSP 2 Score: 42.743 bits (99), Expect = 8.519e-2
Identity = 34/108 (31.48%), Postives = 62/108 (57.41%), Query Frame = 0
Query: 183 APEVTVQRDGRNQSVADTSVPEVAIQRDLEDQFVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQKDDEENQSVDNEYATEIVIQKYVEDQSESD 290
A EV +++D V D EVA+++D+ED+F + EVA D + + E+A+++DV+D+ A+ EV + D V++ +A+E+V++K ED+ S+
Sbjct: 805 ASEVALRKD-----VEDRFASEVALRKDVEDRFAS---EVALRKD-----VEDRFASEVALRKDVEDRFAS---EVALRKD-----VEDRFASEVVLRKDAEDRFASE 891
HSP 3 Score: 41.9726 bits (97), Expect = 1.299e-1
Identity = 36/125 (28.80%), Postives = 67/125 (53.60%), Query Frame = 0
Query: 175 IQSVPQANAPEVTVQRDGRNQSVADTSVPEVAIQRDLEDQFVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQKDDEEN---------QSVDNEYATEIVIQKYVEDQSESD 290
+ + Q A EV +++D V D EVA+++D+ED+F + EVA D + + E+A+++D +D+ A+ EV + D E+ + V++ +A+E+ ++K VEDQ S+
Sbjct: 699 LHDLEQRIASEVALRKD-----VEDRFASEVALRKDVEDRFAS---EVALRKD-----VEDRFASEVALRKDAEDRFAS---EVALRKDVEDRFASEVALRKDVEDRFASEVALRKDVEDQFASE 807
Match: A0A150GGZ2 (Rieske domain-containing protein {ECO:0000259|PROSITE:PS51296})
HSP 1 Score: 39.6614 bits (91), Expect = 8.197e-1
Identity = 34/125 (27.20%), Postives = 57/125 (45.60%), Query Frame = 0
Query: 184 PEVTVQR--DGRNQSVADTSVPEVAIQRDLEDQFVAHPPE------VAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQ----KDDEENQSVDN--EYATEIVIQKYVEDQSESDAGPS 294
PE + R DG + S A VPE + R E P +++ +DGG+ A VPE + R+ DD AAG ++P +++E++ D ++ + + +S+SD G S
Sbjct: 62 PEGLLSREDDGGSTSAAVREVPEGLLSRGDEGGSTGAAPRAVPEGLLSREDDGGSTGAAPRAVPEGLLSRE-DDGQAAGVADLPDCVLAREEEDDGPFDGIVPSGLRVLPEGLLSRESDSDDGGS 185
Match: A0A540MTA0 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 38.5058 bits (88), Expect = 1.707e+0
Identity = 21/72 (29.17%), Postives = 36/72 (50.00%), Query Frame = 0
Query: 68 LSMVALTSFLLKDTHTWTAIWIASSAIFAILYSSY-KACVKMKRAQLMPPVNRRYYRVLGYQVIPIHIIYNV 138
+S+V +T L D+ W+ + +L+ S+ + V M+ AQL P NR YR L + + H+ N+
Sbjct: 708 ISLVCVTGALYLDSRVTIRNWVVFEVNYVVLFPSFVLSPVTMQDAQLFAPPNRDSYRALNSKSVGFHVRANL 779
Match: A0A5D2QID7 ((Uncharacterized protein {ECO:0000313|EMBL:TYI27070.1}))
HSP 1 Score: 37.7354 bits (86), Expect = 2.114e+0
Identity = 25/113 (22.12%), Postives = 45/113 (39.82%), Query Frame = 0
Query: 102 YKACVKMKRAQLMPPVNRRYYRVLGYQVIPIHIIYNVASHWNPRGVVIDTLLIVVGNEDVVVPNVAEVLQDLGIQSVPQANAPEVTVQRDGRNQSVADTSVPEVAIQRDLEDQ 214
Y A ++ PP + +Y L +Q +++ T+ +GN D+ P +A L IQ++PQ E+ + G + S E+ ++R DQ
Sbjct: 18 YSALFDEDEHEIFPPSDDKYAEELQFQ----------------EALMLSTVFSEMGNNDICNPCIAHASSPLLIQALPQPELLEMETEESGE----SSLSFCEICVERKENDQ 110
Match: A0A200QIP3 ((Pre-mRNA polyadenylation factor Fip1 {ECO:0000313|EMBL:OVA10314.1}))
HSP 1 Score: 37.7354 bits (86), Expect = 3.187e+0
Identity = 33/129 (25.58%), Postives = 58/129 (44.96%), Query Frame = 0
Query: 185 EVTVQRDGRNQSVADTSVP-EVAIQRDLEDQFVAHPPEVAKNNDGGTESI------ANANVPEIAVQRDVDDQSAAGEPEVPQKDDEENQSVDNEYATEIVIQKYVEDQSESDAGPSEVAHKDEEYHDL 306
++ V GR+ D +P E+A ++D F A P + + DGG + A + P I R + ++ G VP + + + D++ EIV+Q V+D S ++ G E + + DL
Sbjct: 470 KIRVYESGRSVQDYDPDLPPELAAAAGVQD-FAAENPNLGRT-DGGQGDLTVQGRGAAHSRPLIPTGRAIQVEAGYGAERVPSTESRQPRMRDSDAIIEIVLQDSVDDDSFTENGTHEQLDNELQGEDL 596
Match: K3ZYE8 ((Uncharacterized protein {ECO:0000313|EnsemblPlants:KQL26861}))
HSP 1 Score: 34.6538 bits (78), Expect = 4.027e+0
Identity = 18/62 (29.03%), Postives = 31/62 (50.00%), Query Frame = 0
Query: 46 LIEKIW-SEF--WRNRIKCLEVVFQLSMV---ALTSFLLKDTHTWTAIWIASSAIFAILYSS 101
+I+ IW S F W+ +KC + F+ M+ L +L H+W +W S + A ++S
Sbjct: 14 MIDPIWISTFAAWQRILKCKQTAFKTVMLFAARLVGYLFGVCHSWGKVWSKSKRLLASFFAS 75
Match: A0A6P4M5U3 ((probable E3 ubiquitin-protein ligase RNF217 {ECO:0000313|RefSeq:XP_017605467.1}))
HSP 1 Score: 36.1946 bits (82), Expect = 6.634e+0
Identity = 25/113 (22.12%), Postives = 44/113 (38.94%), Query Frame = 0
Query: 102 YKACVKMKRAQLMPPVNRRYYRVLGYQVIPIHIIYNVASHWNPRGVVIDTLLIVVGNEDVVVPNVAEVLQDLGIQSVPQANAPEVTVQRDGRNQSVADTSVPEVAIQRDLEDQ 214
Y A ++ PP + +Y L +Q ++ T+ +GN D+ P +A L IQ++PQ E+ + G + S E+ ++R DQ
Sbjct: 18 YSALFDEDEHEIFPPSDDKYAEELQFQ----------------EALMSSTVFSEMGNNDICNPCIAHASSPLLIQALPQPELLEMETEESGE----SSLSFCEICVERKENDQ 110
Match: A0A5D2Z7H9 ((Uncharacterized protein {ECO:0000313|EMBL:TYJ34184.1}))
HSP 1 Score: 36.1946 bits (82), Expect = 6.753e+0
Identity = 25/113 (22.12%), Postives = 44/113 (38.94%), Query Frame = 0
Query: 102 YKACVKMKRAQLMPPVNRRYYRVLGYQVIPIHIIYNVASHWNPRGVVIDTLLIVVGNEDVVVPNVAEVLQDLGIQSVPQANAPEVTVQRDGRNQSVADTSVPEVAIQRDLEDQ 214
Y A ++ PP + +Y L +Q ++ T+ +GN D+ P +A L IQ++PQ E+ + G + S E+ ++R DQ
Sbjct: 18 YSALFDEDEHEIFPPSDDKYAEELQFQ----------------EALMSSTVFSEMGNNDICNPCIAHASSPLLIQALPQPELLEMETEESGE----SSLSFCEICVERKENDQ 110
Match: A0A3Q0EV35 ((ABC transporter G family member 22 isoform X2 {ECO:0000313|RefSeq:XP_022635021.1}))
HSP 1 Score: 36.5798 bits (83), Expect = 6.894e+0
Identity = 20/63 (31.75%), Postives = 31/63 (49.21%), Query Frame = 0
Query: 214 QFVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQKDDEENQSVDNEYATE 276
QF+ P + N +AN NV +I+V ++ D+ G EV +D+ + SV EY E
Sbjct: 200 QFIGCAPLITMNPAEFLLDLANGNVNDISVPSELKDRVQVGNAEVETCNDKPSASVVQEYLEE 262
Match: A0A2U1PIW9 ((DNA mismatch repair protein {ECO:0000313|EMBL:PWA85706.1}))
HSP 1 Score: 36.5798 bits (83), Expect = 7.975e+0
Identity = 40/150 (26.67%), Postives = 64/150 (42.67%), Query Frame = 0
Query: 128 QVIPIHIIYNVASH---WNPRGVVIDTLLIVVGNEDVVVPNVAEVLQDLGIQS-VPQANAPEVTVQRD--GRNQSVAD-TSVPEVAIQRDLEDQ-----FVAHPPEVAKNNDGGTESIANANVPEIAVQRDVDDQSAAGEPEVPQKDDEE 265
QV I I+ V S + R V+D + +G D + + L++LG S + Q V D GR S D ++ +Q LE + FV H PE+ G + S+ + +V + Q+D D + G E +D E+
Sbjct: 823 QVALIAIMAQVGSFVPASSARLHVVDGIYTRMGASDSIQQGRSTFLEELGEASHILQTCTDRSLVILDELGRGTSTHDGVAIAYATLQYLLEQKRCMVLFVTHYPEIVNMTKGFSGSVGSYHVSYLTSQKDEDTMKSDGNREGENRDFED 972
| Match Name | Stats | Description |
|---|---|---|
| A0A250WPN8 | E-Value: 2.595e-2, PID: 32.41 | (Uncharacterized protein {ECO:0000313|EMBL:GAX7281... [more] |
| A0A150GGZ2 | E-Value: 8.197e-1, PID: 27.20 | Rieske domain-containing protein {ECO:0000259|PROS... [more] |
| A0A540MTA0 | E-Value: 1.707e+0, PID: 29.17 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A5D2QID7 | E-Value: 2.114e+0, PID: 22.12 | (Uncharacterized protein {ECO:0000313|EMBL:TYI2707... [more] |
| A0A200QIP3 | E-Value: 3.187e+0, PID: 25.58 | (Pre-mRNA polyadenylation factor Fip1 {ECO:0000313... [more] |
| K3ZYE8 | E-Value: 4.027e+0, PID: 29.03 | (Uncharacterized protein {ECO:0000313|EnsemblPlant... [more] |
| A0A6P4M5U3 | E-Value: 6.634e+0, PID: 22.12 | (probable E3 ubiquitin-protein ligase RNF217 {ECO:... [more] |
| A0A5D2Z7H9 | E-Value: 6.753e+0, PID: 22.12 | (Uncharacterized protein {ECO:0000313|EMBL:TYJ3418... [more] |
| A0A3Q0EV35 | E-Value: 6.894e+0, PID: 31.75 | (ABC transporter G family member 22 isoform X2 {EC... [more] |
| A0A2U1PIW9 | E-Value: 7.975e+0, PID: 26.67 | (DNA mismatch repair protein {ECO:0000313|EMBL:PWA... [more] |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
The RefSeq genome records for Daucus carota subsp. sativus were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results. View the full report at https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Daucus_carota_subsp._sativus/100/ Data from this analysis can be viewed in JBrowse here. | |
This analysis is a blastp search of all of the NCBI Daucus carota subsp. sativus Annotation Release 100 polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove DCAR gene predictions from DCAR V1.0 Gene Prediction. |