Query: 18 LPTKTPLNPSLKPFNFTFTCSTTRRRSYVVRAAA--------------ESNDKQNDDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVSNSDDGLFGFLSKRKREEEKVSSRNESGD--------VFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW---FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLP-FRQSQEEESEVEYSML 918
LP P+ + N FT + +R + R A S + DDF+TRVLK+NPSQVEPK+L+G KLYT+KEKE L SD G+F L K + +E D V+LKD+LRE+RGKLYVPEQ+FG N+S+EEEF +NL+ LP+++ EDF KA++SD++K+L+ ED G S+ FRDF+V+LKEIPGDKNLQRTKW MKL ++ A++VL+EY G RYEIE+ Q S VGKLPEYPHPVASSISSR++VELGM+TAVMAAAA V GGFLASAV AVTSF+F +YV WP+AKP LKL +GL+ G++E V +K D+ DGGI SKLYE+Y G V +L +L PI+MVF++M LL+RFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTF+DVAGIEEAVEELQELVRYLKNPELFDK+GIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQG F+DS D LYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKI+I PNAKGRL+ILKVHARKVKLS+S+DLSTYA+NLPGWTGA+LAQLLQEAALVAVRK HNAI+Q D+D+AVDRLTVGPKRV+IELGHQGQCRRATTEVGTA+TSHLLR+ ENA VE CDRISINPRGQTLSQ+VFNRLDDE Y+FERRPQLLHRLQVLLGGRAAEE+IFGRDTS+ASVNYLADASWLARKIITIWNLENP+VIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIE PINFNLDD+VA+RTE+LMRDMY +TVALLR H+AALLKTVKVLLNQKEI+GEEI+FI+ NYP HTPTSLILEE+DPG LP FRQ QE+ E+EY +L
Sbjct: 29 LPISVPIRTHSR--NLNFTQNIGKRSHFFYRPLAILKKTNSKSSSQSDNSTNSTEDDFITRVLKENPSQVEPKYLVGGKLYTVKEKENLKRASDIGVFDILKKLNLKARGKKKNDEGLDENVVKPEAVYLKDILREHRGKLYVPEQIFGANLSEEEEFDKNLEALPKMSLEDFQKAIKSDKIKLLTLKEDTGVSYGYGFRDFIVDLKEIPGDKNLQRTKWAMKLDENLARTVLDEYAGPRYEIER-QTTSWVGKLPEYPHPVASSISSRVMVELGMVTAVMAAAAVVAGGFLASAVFAVTSFLFAVVVYVAWPMAKPFLKLFFGLIFGILERVWDKFVDIFGDGGIFSKLYEVYTFGGVSASLEMLKPIMMVFLTMVLLVRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFNDVAGIEEAVEELQELVRYLKNPELFDKLGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGIFSDSTDHLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIRIRPPNAKGRLDILKVHARKVKLSESVDLSTYARNLPGWTGAKLAQLLQEAALVAVRKGHNAILQSDVDDAVDRLTVGPKRVSIELGHQGQCRRATTEVGTAMTSHLLRRYENAKVEPCDRISINPRGQTLSQLVFNRLDDELYMFERRPQLLHRLQVLLGGRAAEEVIFGRDTSRASVNYLADASWLARKIITIWNLENPMVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIERPINFNLDDDVARRTEELMRDMYGRTVALLRRHHAALLKTVKVLLNQKEISGEEIDFILNNYPRHTPTSLILEEKDPGSLPFFRQKQEQGHELEYRLL 952
Query: 59 DDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVSNSDDGLFGFLSKRKREEEKVSSRNESGD--------VFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW---FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLP-FRQSQEEESEVEYSML 918
DDF+TRVLK+NPSQ+EPK++IG KLYT+KEK L +SD G+F L K + NE D V+LKD+LREYRGKLYVPEQVFG N+S+EEEF RNL+ LP+++ EDF KAM++D++K+L+ ED G S+ FRDF+V+LKEIPGDK+LQRTKW MKL ++ A++VLEEYTG RY IE+ Q S VGKLPEYPHPVASSISSR++VELGM+TAVMAAAA V GGFLASAV AVTSF+F +YV+WP+AKP LKL +GL+ G++E V + D+ SDGGI SKL E+Y G V +L +L PI+MVF++M LL+RFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTF+DVAGIEEAVEELQELVRYLKNP+LFDKMGIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQG F++S D LYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNR DLLDPALLRPGRFDRKI+I PNAKGRLE+LKVHARKVKLS+S+DLSTYAQNLPGWTGA+LAQLLQEAALVAVRK HN+I+Q D+++AVDRLTVGPKRV IELGHQGQC RATTEVGTA+TSHLLRQ ENA VE CDRISINPRGQTLSQVVFNRLDDESY+FERRPQLLHRLQVLLGGRAAEE+IFGRDTS+ASVNYLADASWLARKIITIWNLENP+VIHGEPPPWRKKVKFVGPRLDFEGSLYDDY LIE PINFNLDD+VA+RTE+LMRDMY +TVALLR H+AALLKTVKVLLN+KEI+GEEI+F++ NYP HTPTSLILEE+DPG LP FRQ+QE+ E+EYS+L
Sbjct: 79 DDFITRVLKENPSQIEPKYMIGGKLYTVKEKGNLKRSSDTGVFEILKKLNLKGRAKKKSNEGLDENVVKPEAVYLKDILREYRGKLYVPEQVFGANLSEEEEFDRNLEALPKMSLEDFQKAMKNDKIKLLTLKEDTGVSYGYGFRDFIVDLKEIPGDKSLQRTKWAMKLDENLARTVLEEYTGPRYGIER-QTTSWVGKLPEYPHPVASSISSRMMVELGMVTAVMAAAAVVAGGFLASAVFAVTSFLFAVVVYVVWPMAKPFLKLFFGLIFGILERVWDNFVDIFSDGGIFSKLSEVYTFGGVSASLEMLKPIMMVFLTMVLLVRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFNDVAGIEEAVEELQELVRYLKNPDLFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGIFSESTDHLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRMDLLDPALLRPGRFDRKIRIRPPNAKGRLEVLKVHARKVKLSESVDLSTYAQNLPGWTGAKLAQLLQEAALVAVRKGHNSILQSDVNDAVDRLTVGPKRVGIELGHQGQCHRATTEVGTAMTSHLLRQYENAKVERCDRISINPRGQTLSQVVFNRLDDESYMFERRPQLLHRLQVLLGGRAAEEVIFGRDTSRASVNYLADASWLARKIITIWNLENPMVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYGLIEHPINFNLDDDVARRTEELMRDMYGRTVALLRRHHAALLKTVKVLLNKKEISGEEIDFVLNNYPQHTPTSLILEEKDPGSLPFFRQNQEQGHELEYSLL 949
Query: 26 PSLKPFNFTFTCSTTRRRSYVVRAAA------ESNDK-------QNDDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVSNSDDGLFGFLSKRKREEEKVSSRN--------ESGDVFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW---FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLPF-RQSQEEESEVEYSML 918
P K F + RRRS+ +SN K N+DFVTRVLK+NPSQVEPK+ IG+KLYTLKEKE L +G G L KR K +N S +V+LKD+LREY+GKLYVPEQ+FG N+S+EEEF++N++ LP++ EDF K M+ D++K+L+F ED GAS FRDFVVELK+IPG+K+LQRTKW MKL ++QA+ +LEEYTG RYE+EK Q++S VGKLPEYP+P AS ISSR++VELGMLTAVM AAA +VG FLASAV AVTSFVF A+YV+WP+AKP LKL +GL+ G++E V +KV D +DGGI SKLYE+Y G V ++ +L PI++VF++M LL+RFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTF DVAGIEEAVEELQELVRYLKNPELFDKMGIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVN+PSVIFIDEIDALATRRQG F++S D LYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNR+DLLDPALLRPGRFDRKI+I PNAKGRL+ILKVHARKVKLS ++DL+TYAQNLPGW+GA+LAQLLQEAALVAVR+ HN+I+ D+D+AVDRLTVGPKRV +ELGHQGQCRRA TEVGTALTSHLLR ENANVE CDRISINPRGQTLSQVVFNRLDDESY+FER PQLLHRLQV LGGRAAEE+I+GRDTS+ASVNYLADASWLARKIITIWN+ENP+ IHGEPPPWRKKV+FVGPRLDFEGSLYDDYDLIEPP NF+LDD+VA++TE+L+RDMY KTVALLR+H+AALLKTVKVLLN KEI+G+EI+ I+ +YP +TPTSL+LEERDPG LPF + QE+ + VEYS+L
Sbjct: 39 PHQKSFKNSTFHRNFRRRSHFYHCPCAILGKWKSNSKPSEEKGSNNEDFVTRVLKENPSQVEPKYKIGNKLYTLKEKEDLGKKGLNG--GILEILKRLNFKGLVKNGSDEGSLVNSENVYLKDILREYKGKLYVPEQIFGANLSEEEEFEKNVEDLPKMKLEDFQKYMKFDKIKLLTFKEDTGASLGFGFRDFVVELKDIPGEKSLQRTKWAMKLDQNQAQRLLEEYTGPRYEVEK-QMMSWVGKLPEYPNPAASKISSRVMVELGMLTAVMIAAAVIVGTFLASAVFAVTSFVFAVAVYVMWPVAKPFLKLFFGLIFGILERVWDKVVDAFTDGGIFSKLYEVYTFGGVSASIEMLKPIMLVFLTMVLLVRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFGDVAGIEEAVEELQELVRYLKNPELFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNRPSVIFIDEIDALATRRQGIFSESTDHLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRRDLLDPALLRPGRFDRKIRIRPPNAKGRLDILKVHARKVKLSDTVDLTTYAQNLPGWSGAKLAQLLQEAALVAVRRGHNSILHSDMDDAVDRLTVGPKRVGVELGHQGQCRRAVTEVGTALTSHLLRHYENANVERCDRISINPRGQTLSQVVFNRLDDESYMFERLPQLLHRLQVFLGGRAAEEVIYGRDTSRASVNYLADASWLARKIITIWNMENPMTIHGEPPPWRKKVRFVGPRLDFEGSLYDDYDLIEPPTNFDLDDDVAKKTEELIRDMYGKTVALLRQHDAALLKTVKVLLNHKEISGDEIDLILSHYPQNTPTSLLLEERDPGSLPFLNEKQEQHNNVEYSLL 953
BLAST of XM_017370949.1 vs. ExPASy Swiss-Prot and TrEMBL without DCAR Match: A0A1U7W2W7 ((ATP-dependent zinc metalloprotease FTSH 8, chloroplastic {ECO:0000313|RefSeq:XP_009774397.1}))
Query: 17 HLPTKTPLNPSLKPFNFTFTCSTTRRRSY-----VVRAAAESNDKQ-------NDDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVSNSDDGLFGFLSKR--------KREEEKVSSRN---ESGDVFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW----FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLPFRQSQEEES-EVEYSML 918
+ PT P NP + N+ F T+R + V+ A SN K ++DFV+RVL++NPSQVEPK+LIGDKLYTLKEKEGL + KR K +EE ++ N G+V+LKDLLREYRGKLYVPEQVFG N+S+EEEF +N+ LPR+++EDF K M D++K+L+F E++G S+ FRDFVV+LKEIPGDK L +TKW M+L +Q K +LE YTG R EIEK Q++S VGKLPEYPHPVAS ISSR++ ELG+LTA MAAAA VGGFLASAV AVTSFVF A YVIWP+ KP LK++ GL+ ++E + E + D L D G+ SKLYE+Y G V ++ +L PI++VF++M LL+RFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGV FSDVAGIEEAVEELQELV+YLKNPELFDKMGIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQG F +S D YNAATQERETTLNQLLIELDGFDTGKGVIFLGATNR DLLDPALLRPGRFDRKI+I PNAKGRL+ILKVHARKVKLS ++DLS+YA NLPGWTGA+LAQLLQEAALVAVRK H AI+Q D+D+AVDRLTVGPKRV I+LGHQGQ RRATTEVGTALTSHLLR++ENA VE CDR+SI+PRGQTLSQVVF+RLDDESY+FERRPQLLHRLQVLLGGRAAEE+IFGRDTSKASV+YLADASWLARKIIT+WN+E+P+V+HGEPPPWRK++KFVGPR+DFEGSLYDDYDLI+PPINF LDD+VA+RTE LMRDMY KTVALLR+HNAALLKTVKVLL++KEI G EI+FI+ NYPP TPTSL+LEER+PG LPF + ++ +S E+EY++L
Sbjct: 14 NFPTVFP-NPRILK-NYNFPVEPTKRLQFNSYPRVLLVKASSNAKPSVSSGGGDEDFVSRVLRENPSQVEPKYLIGDKLYTLKEKEGLSKKGFRERVSEILKRLNLKALVSKSDEETGNASNFVKPEGEVYLKDLLREYRGKLYVPEQVFGANLSEEEEFDKNVKELPRMSYEDFQKYMTCDKIKLLTFKEESGISYSNYGFRDFVVDLKEIPGDKRLHQTKWAMRLDVEQVKDLLEAYTGPRNEIEK-QMMSWVGKLPEYPHPVASKISSRMIAELGVLTASMAAAAVFVGGFLASAVFAVTSFVFAVAAYVIWPVVKPFLKIILGLIFSVLERIWENLADFLGDEGLRSKLYEVYAFGGVSASIEMLKPILLVFLTMVLLVRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVMFSDVAGIEEAVEELQELVKYLKNPELFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGMFRESTDDHYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRMDLLDPALLRPGRFDRKIRIRPPNAKGRLDILKVHARKVKLSDTVDLSSYANNLPGWTGAKLAQLLQEAALVAVRKGHAAILQSDMDDAVDRLTVGPKRVGIDLGHQGQSRRATTEVGTALTSHLLRRIENAKVERCDRVSIHPRGQTLSQVVFHRLDDESYMFERRPQLLHRLQVLLGGRAAEEVIFGRDTSKASVSYLADASWLARKIITVWNMEDPMVVHGEPPPWRKRIKFVGPRIDFEGSLYDDYDLIDPPINFKLDDDVARRTEDLMRDMYGKTVALLRQHNAALLKTVKVLLDRKEINGYEIDFILDNYPPETPTSLVLEERNPGSLPFFEDEQSQSKELEYTLL 940
Query: 53 SNDKQNDDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVS-NSDDGLFGFL---------SKRKREEEKVSSRNESGDVFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW---FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLP-FRQSQEEESEVEYSML 918
SND++ DDFVTR+LK+NPSQVEP++LIG+KLYTLKEKE L + N D G+ G L SK + E + S E V+LKD+LREY+GKL+VPEQ+FG N S EEEF++N++VLP+++ EDF K M+SD++K+L+F E+ + + FRDFVVELKEIPG+++LQRTKW M+L + QA+ +LE+YTG R EIEK Q++S VGKLPEYPHP+AS ISSR++VELG+LTAVM AAA VVGGFLASAV AVTSF+F A+YV+WP+ KP LK +G++ G++E V EK D +DGG SKLYE+Y G V ++ +L PI++VF +M +LLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGV FSDVAGI+EAV+ELQELVRYLKNPELFDKMGIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQG F++S D LYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNR+DLLDPALLRPGRFDRKI+I PNAKGRL+ILKVHAR+VK+S+++DL++YA+NLPGWTGA+LAQLLQEAALVAVRK H++I+Q DLD+AVDRLTVGP+RV ELGHQGQC RATTEVGTALTSHLLR+LENA VE CDR+SI PRGQTLSQVVF+RLDDESY+FERRPQL+HRLQVLLGGRAAEE+IFGRDTS+ASVNYLADA+WLARKIITIWNLE P+VIHGEPPPWRK KFVGPRLDFEGSLYDDY LIE P+NFNLDDE+A+RTE+LMR+MY T+ALL+ H AAL KTVKVLLNQKEI+GEEI+FI+ +YPPHTP +LILEE DPG LP F Q Q++++E+EYS+L
Sbjct: 82 SNDEK-DDFVTRILKENPSQVEPRYLIGNKLYTLKEKENLSNKNLDYGVVGLLKTLNLKSLLSKTRYEGQLTKSEEE---VYLKDILREYKGKLFVPEQIFGANFSDEEEFEKNVEVLPKMSIEDFRKYMKSDKIKLLTFKENPASPYGVGFRDFVVELKEIPGERSLQRTKWAMRLDESQAQVMLEQYTGPRNEIEK-QMMSFVGKLPEYPHPIASKISSRVMVELGVLTAVMTAAAIVVGGFLASAVFAVTSFIFAVAVYVVWPVVKPFLKFFFGIIFGVLERVWEKFLDFFTDGGFFSKLYEVYTFGGVSASIEMLKPILLVFGTMVILLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVLFSDVAGIDEAVDELQELVRYLKNPELFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGIFSESTDHLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRRDLLDPALLRPGRFDRKIRIRPPNAKGRLDILKVHARRVKISETVDLASYAKNLPGWTGAKLAQLLQEAALVAVRKGHSSIIQSDLDDAVDRLTVGPRRVGFELGHQGQCCRATTEVGTALTSHLLRRLENAQVERCDRVSIIPRGQTLSQVVFHRLDDESYMFERRPQLVHRLQVLLGGRAAEELIFGRDTSRASVNYLADATWLARKIITIWNLETPMVIHGEPPPWRKSSKFVGPRLDFEGSLYDDYGLIERPVNFNLDDEIARRTEELMREMYAMTLALLKRHQAALFKTVKVLLNQKEISGEEIDFILDSYPPHTPINLILEEGDPGSLPFFSQKQKQDTELEYSLL 956
Query: 20 TKTPLNPSLKPFNFTFTCSTTRRRSYVVRAA-------------AESNDKQNDDFVTRVLKQNPSQVEPKFLIGDKLYTLKEKEGLVSNSDDGL-FGFLSKRKREEEKVSSRNES-------GDVFLKDLLREYRGKLYVPEQVFGQNVSQEEEFKRNLDVLPRITFEDFLKAMRSDQVKVLSFVEDNGASW---FRDFVVELKEIPGDKNLQRTKWTMKLYKDQAKSVLEEYTGTRYEIEKNQLVSSVGKLPEYPHPVASSISSRIVVELGMLTAVMAAAAAVVGGFLASAVLAVTSFVFVTALYVIWPIAKPLLKLLYGLVSGMIEIVGEKVGDLLSDGGINSKLYEIYISGNVVTTLRLLVPIIMVFVSMALLLRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFSDVAGIEEAVEELQELVRYLKNPELFDKMGIKAPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGTFTDSKDQLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRKDLLDPALLRPGRFDRKIKISAPNAKGRLEILKVHARKVKLSKSLDLSTYAQNLPGWTGARLAQLLQEAALVAVRKNHNAIMQPDLDEAVDRLTVGPKRVAIELGHQGQCRRATTEVGTALTSHLLRQLENANVESCDRISINPRGQTLSQVVFNRLDDESYIFERRPQLLHRLQVLLGGRAAEEIIFGRDTSKASVNYLADASWLARKIITIWNLENPLVIHGEPPPWRKKVKFVGPRLDFEGSLYDDYDLIEPPINFNLDDEVAQRTEQLMRDMYDKTVALLREHNAALLKTVKVLLNQKEITGEEINFIIKNYPPHTPTSLILEERDPGGLPF-RQSQEEESEVEYSM 917
TK P S K N TF S R+RS+ + E + N+DF+TRVLK+NPSQVEPK+LIG+KLYTLKEK+ L GL G L K+ K +N S GDVFLKD+LREY+GKLYVPEQ+FG ++S+EEEF++N++ LP++T EDF K ++ D++K+L F ED+GA FRDFVVELKE+PG+K+LQRTKW MKL ++QA+++LEEYTG RYE+EK Q++S VGKLPE P+P AS ISSR+VVEL MLTAVM AAA +VG FLASAV AVTSFVF +YV+WP+AKP LKL +G++ G++E V +KV D +DGG+ KLYE+Y G V ++ +L PI++VFV+M LL+RFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTF+DVAGIEEAVEELQELVRYLKNPELFDKMGIK PHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQG ++S D LYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNR+DLLDPALLRPGRFDRKI+I PNAKGRL+ILKVHARKVKLS ++DL++YAQNLPGW+GA+LAQLLQEAALVAVR+ HN+I+Q D+D+AVDRLTVGP+RV IELGHQGQCRRA TEVGTA+TSHLLRQ ENA VE CDRISINPRG+TLSQVVF+RLDDESY+FER P+LLHRLQV LGGRAAEE+I+GR++S ASV+YLADASWLARKIITIWN++ P+ IHGEP PW KKV+FVGPRLDF GSLYDDYDLIEPPINFNLDD+VA++TE+L+R+MY+KT+ALLR+H+ ALLKTVKVLLN+ EI+G+EI+ I+ +YPP+TPTSL+LEE+DPG LPF + QE+ +EYS+
Sbjct: 29 TKYPYQKSFK--NSTFH-SNFRKRSHFYQCPYAILGKWKSILKPTEEKESNNEDFITRVLKENPSQVEPKYLIGNKLYTLKEKQDL---GKKGLGSGVLEILKKLNIKGMVKNGSDEGSLVKGDVFLKDILREYKGKLYVPEQIFGASLSEEEEFEKNVEDLPKMTLEDFKKYLKYDKIKLLIFKEDSGAYLGFGFRDFVVELKEMPGEKSLQRTKWAMKLDQNQAQTLLEEYTGPRYEVEK-QMMSWVGKLPECPNPAASKISSRVVVELAMLTAVMTAAAVIVGAFLASAVFAVTSFVFAVTVYVVWPVAKPFLKLFFGIIFGILERVWDKVADAFADGGL--KLYELYTFGGVSASIEMLKPIMLVFVTMVLLVRFTLSRRPKNFRKWDIWQGIEFSQSKPQARVDGSTGVTFNDVAGIEEAVEELQELVRYLKNPELFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEFVEVLVGVGSARIRDLFKRAKVNKPSVIFIDEIDALATRRQGILSESTDHLYNAATQERETTLNQLLIELDGFDTGKGVIFLGATNRRDLLDPALLRPGRFDRKIRIRPPNAKGRLDILKVHARKVKLSDTVDLASYAQNLPGWSGAKLAQLLQEAALVAVRRGHNSILQSDMDDAVDRLTVGPRRVGIELGHQGQCRRAITEVGTAMTSHLLRQYENAEVERCDRISINPRGKTLSQVVFHRLDDESYMFERLPRLLHRLQVFLGGRAAEEVIYGRNSSTASVSYLADASWLARKIITIWNMKTPMAIHGEPSPWVKKVRFVGPRLDFGGSLYDDYDLIEPPINFNLDDDVAKKTEELIREMYNKTIALLRQHDTALLKTVKVLLNRTEISGDEIDSILSHYPPNTPTSLLLEEKDPGSLPFVDERQEQHDNIEYSL 942
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.
There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.
LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.
Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.
RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.
The NCBI Sequence report lists the correspondences between the various naming methods
The RefSeq genome records for Daucus carota subsp. sativus were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results.
This analysis is a blastp search of all of the NCBI Daucus carota subsp. sativus Annotation Release 100 polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove DCAR gene predictions from DCAR V1.0 Gene Prediction.
