DCAR_027770 | CarrotOmics

Resource Type:

mRNA

Name:

DCAR_027770

Identifier:

DCAR_027770.mRNA

Organism:

Daucus carota subspecies sativus

Analysis:


Name	Description
Carrot Genome Assembly DCARv2	An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below. These sequences can be viewed in JBrowse here. Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search
Carrot Genome Assembly V.2 RNAseq population 70796 dOr vs. pOr	This analysis was part of the carrot genome assembly publication In population 97837, root tissue was collected from plants with yellow (yyY₂Y₂) and white (YYY₂Y₂) genotypes, with two biological replications per genotype, at 80 days after planting (DAP). In population 70796, root tissue was collected from plants with dark orange (yyy₂y₂) and pale orange (YYy₂y₂) genotypes, with three biological replications per genotype, at 100 DAP. Total RNA was extracted from whole root tissue using the TRIzol® Plus RNA Purification Kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol. RNA was treated for DNA contamination with the TurboDNA-free kit (Life Technologies, Carlsbad, CA). RNA quantity and integrity was confirmed with an Experion RNA StdSens Analysis kit (Bio-Rad, Hercules, CA). All samples had RQI values above 8.0. For each biological replicate, a 133 nt insert size paired-end library was prepared at the Biotechnology Center, UW-Madison (WI, USA). Libraries were sequenced on Illumina HiSeq2000 lanes using 2 × 100 nt reads. Reads were filtered using Trimmomatic version 0.32 with adapter trimming and using a sliding window of length ≥50 and quality ≥28, i.e. “ILLUMINACLIP:adapterfna:2:40:15 LEADING:28 TRAILING:28 SLIDINGWINDOW:10:28 MINLEN:50”. Filtered reads were aligned to the Daucus carota v2.0 genome assembly using the program TopHat v2.0.12 (ref. 30). Non-default parameters used were “--mate-inner-dist -67 --mate-std-dev 50 --min-intron-length 20 --max-intron-length 10000 --library-type fr-unstranded --num-threads 14”. The aligned read files were processed by Cufflinks v2.2.1 (ref. 61). Reads were assembled into transcripts with “cufflinks” using the carrot annotation v1.0 gene predictions as the reference gtf guide. Samples were combined with “cuffmerge”, and then differential expression analyzed with “cuffdiff”, using non-default parameters of “--multi-read-correct --min-alignment-count=5”. Using the abundance estimations, this performs tests for differential expression and regulation between the samples. Normalized counts of the mapped RNA sequences were used to calculate the relative abundances of transcripts expressed as Fragments Per Kilobase of exon per Million fragments mapped (FPKM). When testing for differential expression, biological replicates were included as a term in the mixed model analysis to account for experimental error. Testing for differential expression was done at the level of genes, isoforms, and promoters. Some of the data from this analysis were published in Sup. Table 44: Annotation of carrot genes upregulated in both yellow and dark orange (yy) storage roots of plants from mapping populations and in Sup. Table 45: Annotation of carrot genes downregulated in yellow or dark orange (yy) storage roots of plants from mapping populations of the carrot genome assembly publication
Carrot Genome Assembly V.2 RNAseq population 97837 Y vs. W	This analysis was part of the carrot genome assembly publication In population 97837, root tissue was collected from plants with yellow (yyY₂Y₂) and white (YYY₂Y₂) genotypes, with two biological replications per genotype, at 80 days after planting (DAP). In population 70796, root tissue was collected from plants with dark orange (yyy₂y₂) and pale orange (YYy₂y₂) genotypes, with three biological replications per genotype, at 100 DAP. Total RNA was extracted from whole root tissue using the TRIzol® Plus RNA Purification Kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol. RNA was treated for DNA contamination with the TurboDNA-free kit (Life Technologies, Carlsbad, CA). RNA quantity and integrity was confirmed with an Experion RNA StdSens Analysis kit (Bio-Rad, Hercules, CA). All samples had RQI values above 8.0. For each biological replicate, a 133 nt insert size paired-end library was prepared at the Biotechnology Center, UW-Madison (WI, USA). Libraries were sequenced on Illumina HiSeq2000 lanes using 2 × 100 nt reads. Reads were filtered using Trimmomatic version 0.32 with adapter trimming and using a sliding window of length ≥50 and quality ≥28, i.e. “ILLUMINACLIP:adapterfna:2:40:15 LEADING:28 TRAILING:28 SLIDINGWINDOW:10:28 MINLEN:50”. Filtered reads were aligned to the Daucus carota v2.0 genome assembly using the program TopHat v2.0.12 (ref. 30). Non-default parameters used were “--mate-inner-dist -67 --mate-std-dev 50 --min-intron-length 20 --max-intron-length 10000 --library-type fr-unstranded --num-threads 14”. The aligned read files were processed by Cufflinks v2.2.1 (ref. 61). Reads were assembled into transcripts with “cufflinks” using the carrot annotation v1.0 gene predictions as the reference gtf guide. Samples were combined with “cuffmerge”, and then differential expression analyzed with “cuffdiff”, using non-default parameters of “--multi-read-correct --min-alignment-count=5”. Using the abundance estimations, this performs tests for differential expression and regulation between the samples. Normalized counts of the mapped RNA sequences were used to calculate the relative abundances of transcripts expressed as Fragments Per Kilobase of exon per Million fragments mapped (FPKM). When testing for differential expression, biological replicates were included as a term in the mixed model analysis to account for experimental error. Testing for differential expression was done at the level of genes, isoforms, and promoters. Some of the data from this analysis were published in Sup. Table 44: Annotation of carrot genes upregulated in both yellow and dark orange (yy) storage roots of plants from mapping populations and in Sup. Table 45: Annotation of carrot genes downregulated in yellow or dark orange (yy) storage roots of plants from mapping populations of the carrot genome assembly publication
DCAR V1.0 Gene Prediction	For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here.

Transcript Id:

gnl|USDA|mrna.DCAR_027770

Protein Id:

gnl|USDA|DCAR_027770

Product:

hypothetical protein

Sequence:

ATGGATTATGATGACAAAATTTCTGAAGGCCATAAGTCAGCTAATGCAGA
GAATTCCAAGTCATCACCCGTGGTGCCTCCATTTGCTCTTCCCAAGTATG
ATTTTGATGATAGCCTACAGGGTGACCTAAGATTTGAAGGATTGGTCGAA
AATGAAGTTTTTCTTGGCATTTCGAGTCAAGAAGACAGCCAGTGGATTGA
GGACTTTTCTAGAGGAAATAATGGTATAGAATTTAGTTCATCTGCGACAG
AGCCTTGTTCCATTTCAAGGCATAACAATGTTTGGTCAGAGGCAACATCT
TCAGAGTCTGTTGCGATGCTTCTGAAGTCCGTTGGACAGGAGGAAAGGAT
TCAGGGAGAAACTTTGATTCAGGAGTTGGACACTGGCAATGAATTAGGCA
ATTTAGCTACAGAAATGGAGCCTAACTTGAAGAAGGACGATAAGGTGGAG
TCTATAACTACCTCACAACCTACTGCGACTCCTGATTCTTTTCTGGATCG
GAGGAATGTGAATGCTGTAGAATCAAAAAAGGATAATGTTCCTGTTGATG
GAAGTTCTGGCGTTGTTTCAATTGTTTCCAGTGTAATTCTAAGCGAGGCA
GATGAGGGACCAGATGATAAATGTGATGATACTACACATTTGAAAGTTAA
TATTATAGACCAGCCCGTTGACAATTTAGAAGGCTGCAACATTGACCAAG
GTTCTGCAGTGACTTCTCAGCTGGGTAATGTTGAAGTCGGGGCCTGCAAC
AAAGGTGAAAAGACTAGTGAGCCAATTCAAAATCCTCTGGGTGACACCTC
ATCTGTGGCTTGTGTAGATGCTCATACCTTTGAGGTTGTGAATAAAGATG
CCGAGTATTCTGCCTGTACTGGGGTCGAAGTCAGTATTGTTGAATTAAAG
GAGAACTCTTTCGGCAACCAACCAAACAGTCTGGAAAGACAGAATGTTGA
GGCCGTTGACATTAAGACAGTAAATTCTTCCTGTCCAGAGCATGATATGG
ACTCGGTGGTTGAGAATGAGAATTCTGAAAACTGTAGTGGGGGAGTGGCT
GTTGCTGAAGCTTCTTCGTTGCTTGCTGCAGACCATAAATTTACTGAACA
GGTGGAAGGAAGTGGCATTGAATATGCTGAGAATCCGGATACATCTGTAT
CTTGCATTGTCTCTGGATCACAATGTGGAATACAGTTGTCTTCTAAGGAT
TCAAGTGATCTCAGAACATCTGGAGTCCTTGAGGATAATAATTCTCCTGA
AGCACAAACATCCTCTTTCGTAGTTGGTGGACATGTGCAGATGAGCAAAA
GTCATATTTCCTCTGGGCAGGAAGATGTCCACCTTTTTGACAAAGATGTA
TCTTCTCTTGGGACAGAAGATGCAAAGTCACTCACAACATCCCTGCCTCC
TGATGAATCTAATAAAGCAAACATAGTTCAAGGAGCAGAACTCCTTAATT
CTGCGAGTAATGAGCGAGGATCAGAAGTTACAGACGAAATGAACTTATCA
TCGTGTGGCATGCCGATTGATCCTCCATTGCTTCACGGGAGCAAGACTAC
TGCTGATGGAGCTACTTTTGGTGAGCAAGAGCATAAGTCACAGGTTGCTA
ATTTATTAGGCACAGAGAGTTCACCACAAGTGTCAGCTGATCTAGAGCCT
TTTAGTTTTCCTGAATCTGGACAAGCAAGTCGGATAAATCAAGAAGCAGG
TCCGGAGGGTTCCAAAAAGGTGGAAGCATGTCCATCTCTAATTAGTTCAG
AAATAAAGCTGGTTGACGATGCTGCACTAGCAGCACATAAAAGCAATGAA
GAAATATATTTGGAAAGAAGTGCTGAATTGGCATCGTCTGAAGTTGCAGA
TGCGGTGTTACCATCAAGCCAAACTCTTGTTCCTGTAGAATCTGTTCCAG
TCTCCATGCTAGAATCCTCTACTATTTCCCAAAAGAATGACGATGTTACA
GCAACTTCCGGAACAGAATTAACAGAAAATCCTAGCACTAAAGTTGATTC
ACCTGAGAAGAATGAAGATTCTGTTTCTCTTGCTTGCCCGTCAAAATGCA
TTTCAGATATTGGGAACATGGATGGAGATGCCTCTGCTAATTCATTTGAA
GAAAATGTGTATACTCCTGCTATCATCAGTTGTGCAAAGGCTCCGCTAAG
TGAAAAGGAACAGAAAACAAGAACTATAGAGGTCTCAATTGGTGATGTCC
AGCATCCTGAGGCAACAGATGGTGCTGCTGAAATGGTTCAATCTGTTCCA
TTGGATGCTACTTTCAACAATGGGGAAATCAGTACTTCTCAGGTTAACAT
GTCAACTGGTTTAACAGAGGGAAAAATCATGAAGGATATGCAATTAAATC
CTGTTCAGGTTCACGAAAGATCAACAGATGTCAAGGAGTATCCATCTGAT
GGTAATATGGGACCTCCCTCTGTTGTCAGTTGTGTAAAGATTCCGATAAG
TGAAACTGGACAGACGTCGAGAGGCAAAGATGCCTCAGTCTCCACTGTCC
ATCATTCTGAAGGAACAGCTGGTGCTGGTGAAAATGTTCAATCTGCTGCA
TTGCATATGGAACATGATAATTCCAATGAAGGGACAAACATCAGTATGGA
GGTCAGTTCATCTACTGGTTTATCTGAAAGCGAAATCATCAAGGGTTCAC
CATCATATCCGAGCATCCCAGTCAACAAAATATCAACTGATGTTGAGGGA
TATCCTTTAGCAGCTGATTGCTCTCGGATAGATCTTACAATGCCTGAACA
TTCTCTTGAAAATTCTGAAGGACCTGGACCTATGTCAGGTAAGAAACGTG
CAGGTGCCAGAGGGACCTCTGAGCGGAAAACAAGACTAGGTTCTGTTAAG
ACAACAGCTAGAACAAATAGTAGGAAAAGAGGTATGCAGGAAACACCTTC
GAAACAGTTTGACGAAGATAAATCAATGATGATCTCAAGTTTACCTGGGA
ATAGCAATGCTGAACTAAGTGGTATAAAAGTTTGTGGCGCCCTTCCTACT
CCCATGTCTACTATTCCAGATTTAAACACTTCAGTTACTCCCGCATATTA
TCAACAACCTTTCACTGATACCCAACAAGTGCAATTGCGAGCACAGATAC
TTGTATATGGATCACTAATGCAAAATTCCGTACCTGACGAGGCTTGTATG
ATTGCAGCATTTGGGCCATCTGATGGTAGTGGTGGATGTCTTTGGGAGTG
TGCATGGCGTGCTTGTAGTGAAAGGATACAAACTCAGAAATCAGATGCCA
GCAACCTGGAAACCCCTGTGCGATCATTCTCTGGCAATTGTGATATAATG
ATAGCAGGTAGCAAAGGTTCGGATCAACCAGTTAAAAAAAGTAAAAGTTC
ACGTCAGAGTAAATCTACTTTACCTGCAGATAGAGCTAGCAGTAAGGATA
TCCCATCGCCAGTTGTTAATCCTATTATACCCCTATCATCACCTCTTTGG
AACGTATCTACCCCCTCTGGTGTTGGTTTGCAGTCCAGTGGCACATATGT
TGATTATCATCAGCCATTTGGTCCCTTGCAGCCTTGTCAGCCCCAAGGTA
CAAAAGGTTTTGGTGAACAGAACCCCTCTTGGCTTTCTCAGATTAACTTT
CCTGGTCAGTGGGTTGCTACTCCACAAACTTCATCCCTTGATGTCAATGC
ATCTGTATCTTCAAAGTTTTGTACTGAACCAGTAAAACTAACCCCATCCA
AAGTTTCATCTGTGCCAAGTGACCCTGCCAGAAAGCAAACCCAATTTTCT
GTTTCCACTGGTGGGAGTGCTGGGCTGTCTTCAGGGATTCATTTTCAGAC
TGATGTAACAAAGACTTCTGTTCTGCCTGGTCAGATTTGTGATAATACTA
AACATAAGAAGAGGAAGAAGGTTTCTGTCAGCAAGAGCCCAGGGCAAATC
CCGTCACTAACTTTAGATCAGGCAGCATCTTTAAGTGATACAAGTAATCA
TATCTCTAAAAAAGGAACTGCTCCCCAGGCTATTTTCCAGAATTCTTTGC
TCGCTCCAAGTCTGCCAGAAATGAGATCCCCTGCTGTCAATAATGCATCA
TCTATGCCAAATGCAGCCACAGCTACTTTTTTGGGATCAACAGGCCGTTC
CAGCAACAATCTACCAGCTGTCTATCCATTAGTTTCGAGTGATCAACCTA
CGAGAGTGGATCAGATTGTGGATAAGAATAGTATAATGTCAGAGGAATCA
CTGAATGCAGTCGAGGCAGCTAGGTTACAAGCAGAAGGTGCTGCTGCACA
TGCTGCTGCTGCAGTCGGAAACTGCAATGGTGTTTGGAGCCAGCTGAGTA
AGCTGAAGAATTCTGGTTTAAGATTTGATGATGAGACAAAACTTTCTTCT
GCAGCTATGGCAATAGCTGCAGCTGCTTCTGTTGCAAAGGCTGCAGCTGC
AGCAGCCAAACTTGCATCGAATATTGCAATTGGAGCAAAACTAATGGCGG
ATGAGGTATCAACCGCAAGTGTAACCAGAAAAGATCCCCATAGCACTGGA
ATGTCTATGGCCTGTTCTTCAAATAGTTCTCTGTTGGCTGGAGAAGGAAA
TGTCTCCACTAGTTCAATTATTACTGCTGCGAAAGAGACAGTTAAGAAGA
GAGTCGAAGCTGCCACAGCTGCCTCAAAGCATGCTGAAAACTTGGATGCT
ATGGTTAAAGCTGCTGAACTGGCAGCAGAGGCCGTTTCACAAGCCGGAAA
AATTGTTTCTATTGGCGATCCCTTGCCTTTGAGTGAGTTGTTAGGCTCTG
GTCCTATGGACTACTGGAGAACTCCCCAGGTATTTCCTGAACAAGGGGTG
ATAGCAGGCATTGACCAACGGAAAACATTTGTTCCTGAAAAGCTTGTTCC
TGCTCAACCACCAAAAGATGGAGCAATTGTTACGGGAGAAGCAGTGGATC
GTGGTACTATTGCAAGTGTTGGAAAGAACTCATTGTCTACTCAAAGAGGC
CACCGTGCATCTGACCCTGCTCTTGATTCATATGGGGGAACAAACTCTGA
AATTGTTAAATCTCGTGATGCCCAAAGAATTTCACCACTTACATCTCTTG
AGAATGTAATGGTGGAAGGTTGCTTTGTCGAGGTCTTCAAAGAAGGGGGT
AAATATAAGGCAGCATGGTATCCAGCCAAGATTTTGAATTCGGATGACGA
GAAAGCTTTTGTCTGTTACACTGAACTCCAAGCAGATGATGGGTCAGGGA
AACTAAAGGAATGGGTTCCACTTAAAGGGGATAACTCCAAGGCACCTATA
ATACGTATTCCACATCCTGCAACTAGCATGTGCTTTGAAAGAAGTAGGAA
GAGACGGAGAGCGGCAGTGATGGACTATACTTGGTGTGTTGGAGACAGAG
TTGACGCGTGGATGCAAGATTGCTGGCTTGAGGGAGTTGTGTCTGAGAAG
AAAAATGATGAAACAACATTTAAAATATCTATTCCAGCTCTTGGAGGCAC
TTCAGCTGTTAAAACATGGAATCTTCGCCCAACCGTAACTTGGCAGGATG
GAAAGTGGATAGAGTGCCCCTCTGCAGTTCAGCAGTCCCCTACTCAGGGT
GATACACCACAGGAGAAGCGTGTGAAGTTGGGACATCCCGCTGTAGAGGC
TGCTAAGGTGGACAAGAGTTTAAGAGGCAATGATCTCATGGAATCAAAGG
AGCATGATGAATCAAGACAACTTCCTTTACTAGCTAGTGAAAAAGAATTT
AACATTGGGAATAATACTGGAGATATTAAACTTGATGCTCCAAGAATTGC
AAGGACTGGATTGCAGAAAGAAGGCTCAAGGGTGGTTTTTGGTGTTCCTA
AGCCAGGAAAGAAGCGTAAATTCATGGATGTAAGCAAACATCTTGACTCT
GACCAATGCAATAAAAATGCAAAAACAAGCGATTTAGTCAAAACTGCAAG
ATACTTGCCTCAAGGAAGAGGGGGTGGTCGTGGTTGGAATAACAATACTA
AAGATGATATCAAAGAAAAGCAAGCTAAAGAAGACAAGGTCAAAGTTATC
AGGTCTGGAGTGGCACAGAAGACTAATGCTTTGCTATCTGCCAAATCTAT
GGGGAAAACTGTCAGCAAGGAACAGAACAATCCAGCTCTGCGGGACCTTA
TGGAATTTGGCTCCGTCTCTAATTCCCCTTCTGAAGGTCCAACTTCTTCA
AAAGGTCCTAGAGTTCCAAATAAAAGAGTACCCTCTTCAAGTACCAAAAC
CGTCCTTCTGAATAAAGGAAAGCATGCAGCTGGTGGAGAGGTTGATAAGA
AAGTTGATCCGCATGTTACTGAGATTCGAAGATCAAACCGGAAGATTCAA
CCAACATCACGGCTATTGGAAGGATTGCAGAGCTCTCTAATCGGTTCAAA
GATGCCCTCTGTCCCGCATGAAAAAGTTCAAAAAAACCTGAACAGAGGTA
ATAATATTAAAATTTGA

Sequence Length:

6417

Sequence Checksum:

3f5bc2544274c022512b83ca3cf34ff1

View location in JBrowse:

DCARv2_Chr8:19538509..19548761-

Protein Sequence:

MDYDDKISEGHKSANAENSKSSPVVPPFALPKYDFDDSLQGDLRFEGLVE
NEVFLGISSQEDSQWIEDFSRGNNGIEFSSSATEPCSISRHNNVWSEATS
SESVAMLLKSVGQEERIQGETLIQELDTGNELGNLATEMEPNLKKDDKVE
SITTSQPTATPDSFLDRRNVNAVESKKDNVPVDGSSGVVSIVSSVILSEA
DEGPDDKCDDTTHLKVNIIDQPVDNLEGCNIDQGSAVTSQLGNVEVGACN
KGEKTSEPIQNPLGDTSSVACVDAHTFEVVNKDAEYSACTGVEVSIVELK
ENSFGNQPNSLERQNVEAVDIKTVNSSCPEHDMDSVVENENSENCSGGVA
VAEASSLLAADHKFTEQVEGSGIEYAENPDTSVSCIVSGSQCGIQLSSKD
SSDLRTSGVLEDNNSPEAQTSSFVVGGHVQMSKSHISSGQEDVHLFDKDV
SSLGTEDAKSLTTSLPPDESNKANIVQGAELLNSASNERGSEVTDEMNLS
SCGMPIDPPLLHGSKTTADGATFGEQEHKSQVANLLGTESSPQVSADLEP
FSFPESGQASRINQEAGPEGSKKVEACPSLISSEIKLVDDAALAAHKSNE
EIYLERSAELASSEVADAVLPSSQTLVPVESVPVSMLESSTISQKNDDVT
ATSGTELTENPSTKVDSPEKNEDSVSLACPSKCISDIGNMDGDASANSFE
ENVYTPAIISCAKAPLSEKEQKTRTIEVSIGDVQHPEATDGAAEMVQSVP
LDATFNNGEISTSQVNMSTGLTEGKIMKDMQLNPVQVHERSTDVKEYPSD
GNMGPPSVVSCVKIPISETGQTSRGKDASVSTVHHSEGTAGAGENVQSAA
LHMEHDNSNEGTNISMEVSSSTGLSESEIIKGSPSYPSIPVNKISTDVEG
YPLAADCSRIDLTMPEHSLENSEGPGPMSGKKRAGARGTSERKTRLGSVK
TTARTNSRKRGMQETPSKQFDEDKSMMISSLPGNSNAELSGIKVCGALPT
PMSTIPDLNTSVTPAYYQQPFTDTQQVQLRAQILVYGSLMQNSVPDEACM
IAAFGPSDGSGGCLWECAWRACSERIQTQKSDASNLETPVRSFSGNCDIM
IAGSKGSDQPVKKSKSSRQSKSTLPADRASSKDIPSPVVNPIIPLSSPLW
NVSTPSGVGLQSSGTYVDYHQPFGPLQPCQPQGTKGFGEQNPSWLSQINF
PGQWVATPQTSSLDVNASVSSKFCTEPVKLTPSKVSSVPSDPARKQTQFS
VSTGGSAGLSSGIHFQTDVTKTSVLPGQICDNTKHKKRKKVSVSKSPGQI
PSLTLDQAASLSDTSNHISKKGTAPQAIFQNSLLAPSLPEMRSPAVNNAS
SMPNAATATFLGSTGRSSNNLPAVYPLVSSDQPTRVDQIVDKNSIMSEES
LNAVEAARLQAEGAAAHAAAAVGNCNGVWSQLSKLKNSGLRFDDETKLSS
AAMAIAAAASVAKAAAAAAKLASNIAIGAKLMADEVSTASVTRKDPHSTG
MSMACSSNSSLLAGEGNVSTSSIITAAKETVKKRVEAATAASKHAENLDA
MVKAAELAAEAVSQAGKIVSIGDPLPLSELLGSGPMDYWRTPQVFPEQGV
IAGIDQRKTFVPEKLVPAQPPKDGAIVTGEAVDRGTIASVGKNSLSTQRG
HRASDPALDSYGGTNSEIVKSRDAQRISPLTSLENVMVEGCFVEVFKEGG
KYKAAWYPAKILNSDDEKAFVCYTELQADDGSGKLKEWVPLKGDNSKAPI
IRIPHPATSMCFERSRKRRRAAVMDYTWCVGDRVDAWMQDCWLEGVVSEK
KNDETTFKISIPALGGTSAVKTWNLRPTVTWQDGKWIECPSAVQQSPTQG
DTPQEKRVKLGHPAVEAAKVDKSLRGNDLMESKEHDESRQLPLLASEKEF
NIGNNTGDIKLDAPRIARTGLQKEGSRVVFGVPKPGKKRKFMDVSKHLDS
DQCNKNAKTSDLVKTARYLPQGRGGGRGWNNNTKDDIKEKQAKEDKVKVI
RSGVAQKTNALLSAKSMGKTVSKEQNNPALRDLMEFGSVSNSPSEGPTSS
KGPRVPNKRVPSSSTKTVLLNKGKHAAGGEVDKKVDPHVTEIRRSNRKIQ
PTSRLLEGLQSSLIGSKMPSVPHEKVQKNLNRGNNIKI*

Publication:

Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.

Relationship:

There are 2 relationships.
Relationships
The mRNA, DCAR_027770, is a part of gene, DCAR_027770.
The polypeptide, DCAR_027770, derives from mRNA, DCAR_027770.